BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0359.0009.p (350 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q5WK46.1 Q5WK46_BACSK Two-component sensor histidine kinase of n... 420 e-115 B3KJ66.1 B3KJ66_9BACI ATP-binding region ATPase domain protein 419 e-115 C2LWN8.1 C2LWN8_STAHO Two component sensor histidine kinase 327 8e-88 Q7WZY5.1 NREB_STACT Oxygen sensor histidine kinase nreB EC=2.7.13.3 322 3e-86 Q4L8Q7.1 NREB_STAHJ Oxygen sensor histidine kinase nreB EC=2.7.13.3 318 7e-85 >Q5WK46.1 Q5WK46_BACSK Two-component sensor histidine kinase of nitrate reduction Length = 359 Score = 420 bits (1079), Expect = e-115, Method: Compositional matrix adjust. Identities = 200/343 (58%), Positives = 266/343 (77%), Gaps = 2/343 (0%) Query: 2 DSLNTLMLKLFENSTEAIFFFDREGRTLAMNPAAAHIVDTDFLKQLEQGNSLALCSTCRG 61 + L+ LM KL+ NS EA+ F D EG + MNPAA I+D ++++ G + C TC+G Sbjct: 7 EHLSELMNKLYRNSMEAMIFIDEEGNVIDMNPAAEQIIDPQVIRRIRAGTDESFCQTCKG 66 Query: 62 YTSDMELRTCLNCFFHVPDSEDFTSYQVYLETKNKGVIPFAASFHTIDSENGIRVLMLRD 121 YT + EL +C NC+ P E F+S+Q++L+TK+ V+PFAAS+HTID E G+RVLML D Sbjct: 67 YTDEQELISCANCYLDYPQGE-FSSFQLFLKTKDGAVVPFAASYHTIDEEAGVRVLMLLD 125 Query: 122 LTRQFKTQEKFYQNKMMKHVIEAQENERKRISRELHDSVAQELMSAVVDLRVLKYMTGDE 181 LT+Q KTQE +N M+K+VI+AQE+ERKRISRELHDSVAQEL+S++VDLRV+KYM E Sbjct: 126 LTKQQKTQEMLQRNHMLKYVIKAQEDERKRISRELHDSVAQELLSSLVDLRVVKYMDVGE 185 Query: 182 QLLKKVKQTEVSMTRLLSDIRNLSVELRPAALDDFGLEAAFRSHFKRMEQIYGLMIEFES 241 +LKK++ TE S+TRLL DIR+L+VELRP++LDD GLEAAFRSHFK ME+ YGL+++F + Sbjct: 186 DVLKKLQHTEQSLTRLLDDIRHLAVELRPSSLDDLGLEAAFRSHFKWMEKNYGLLVKFSA 245 Query: 242 DLSEKRYGSEIETVVYRVCQEALLNALKYAQVDLVHVKLTERDRLLKLTIEDEGVGFNLG 301 +L+ RYGSEIETVVYRVCQEA+LNALKYA+ D V+V+L E L+L + DEGVGF++ Sbjct: 246 ELAAARYGSEIETVVYRVCQEAVLNALKYAETDEVYVRLCEEHGQLQLVVRDEGVGFDMV 305 Query: 302 -DEPDGTGLGLYGMQERAELVGGIFSVESGIGLGTRIIIQVPV 343 +E GTGLGLYGM+ER ELVGG F++ S +G GT + + +P+ Sbjct: 306 INEAKGTGLGLYGMRERTELVGGQFTIHSAVGEGTTVQLSIPL 348 >B3KJ66.1 B3KJ66_9BACI ATP-binding region ATPase domain protein Length = 359 Score = 419 bits (1078), Expect = e-115, Method: Compositional matrix adjust. Identities = 200/350 (57%), Positives = 270/350 (77%), Gaps = 2/350 (0%) Query: 2 DSLNTLMLKLFENSTEAIFFFDREGRTLAMNPAAAHIVDTDFLKQLEQGNSLALCSTCRG 61 + L+ L+ +++NS EAIFF D +G +AMNPAA +I+D + ++++ G + C TC G Sbjct: 7 EHLSELLRNMYQNSMEAIFFMDEDGSVVAMNPAAENILDIEVIERMHSGLDGSFCQTCEG 66 Query: 62 YTSDMELRTCLNCFFHVPDSEDFTSYQVYLETKNKGVIPFAASFHTIDSENGIRVLMLRD 121 YT + +L +C C+ PD E F+S+QVYL+T+++G++P+AAS+HTID GIRV+ML D Sbjct: 67 YTDEQDLISCSKCYLDYPDGE-FSSFQVYLKTRSEGIVPYAASYHTIDEARGIRVMMLLD 125 Query: 122 LTRQFKTQEKFYQNKMMKHVIEAQENERKRISRELHDSVAQELMSAVVDLRVLKYMTGDE 181 LT+Q KTQE + M+K+VI+AQE+ERKRISRELHDSVAQEL+S++VDLRV+KYM E Sbjct: 126 LTKQQKTQEMLQRTNMLKYVIKAQEDERKRISRELHDSVAQELLSSLVDLRVVKYMNVGE 185 Query: 182 QLLKKVKQTEVSMTRLLSDIRNLSVELRPAALDDFGLEAAFRSHFKRMEQIYGLMIEFES 241 +LKK+ QTE S+TRLL DIR+LSVELRPA+LDD GLEAAFRSHFK + + YG+++EF + Sbjct: 186 DVLKKLHQTEASLTRLLDDIRHLSVELRPASLDDLGLEAAFRSHFKWIGKNYGMLVEFSA 245 Query: 242 DLSEKRYGSEIETVVYRVCQEALLNALKYAQVDLVHVKLTERDRLLKLTIEDEGVGFNL- 300 +L+ RYGSEIETVVYRVCQEA+LNALKY+ D VHV+L E + L+LT+ D+GVGFN+ Sbjct: 246 ELAAARYGSEIETVVYRVCQEAVLNALKYSGTDEVHVRLCEENGCLQLTVRDDGVGFNMI 305 Query: 301 GDEPDGTGLGLYGMQERAELVGGIFSVESGIGLGTRIIIQVPVADGNGKE 350 DE GTGLGLYGM+ERAELVGG F++ S G G + +++P+ E Sbjct: 306 IDEAKGTGLGLYGMRERAELVGGQFTLVSAPGEGATVQLKIPLTSSGKAE 355 >C2LWN8.1 C2LWN8_STAHO Two component sensor histidine kinase Length = 344 Score = 327 bits (839), Expect = 8e-88, Method: Compositional matrix adjust. Identities = 162/337 (48%), Positives = 230/337 (68%), Gaps = 4/337 (1%) Query: 7 LMLKLFENSTEAIFFFDREGRTLAMNPAAAHIVDTDFLKQLEQGNSLALCSTCRGYTSDM 66 L+ K +E + E I F ++EG+ +AMN AA I+ D + A+C C GY+++ Sbjct: 12 LLKKYYETTNEKIVFVNKEGKIIAMNDAAEEIISKD---NNYSAMTNAICDRCEGYSNEF 68 Query: 67 ELRTCLNCFFHVPDSEDFTSYQVYLETKNKGVIPFAASFHTIDSENGIRVLMLRDLTRQF 126 EL++C NC+ D ++QV+++T + V PF AS+ ID +N I L+D++ Q Sbjct: 69 ELQSCKNCYLDTSKPNDI-NFQVFMKTVDHKVRPFTASYQAIDEDNEIYAFTLQDISPQI 127 Query: 127 KTQEKFYQNKMMKHVIEAQENERKRISRELHDSVAQELMSAVVDLRVLKYMTGDEQLLKK 186 + QEK YQ +MM+ I AQENERKRISRELHDSV QE+++ V+LR+LKY +L+++ Sbjct: 128 ERQEKMYQRQMMRKTISAQENERKRISRELHDSVVQEMLNIDVELRLLKYQKESSELVQQ 187 Query: 187 VKQTEVSMTRLLSDIRNLSVELRPAALDDFGLEAAFRSHFKRMEQIYGLMIEFESDLSEK 246 ++ E M++L+ DIR+LSVELRP++LDD GLEAAF+S+FK+ E YG+ I ++S++ +K Sbjct: 188 SQRIEGLMSKLIDDIRHLSVELRPSSLDDLGLEAAFKSYFKQFENNYGMQIVYQSNIKDK 247 Query: 247 RYGSEIETVVYRVCQEALLNALKYAQVDLVHVKLTERDRLLKLTIEDEGVGFNLGDEPDG 306 RY SE+ETVVYRV QEA+ NALKYA V V V + E RLL + D G GF+ +P G Sbjct: 248 RYDSEVETVVYRVVQEAVFNALKYAGVSSVDVFVQEVGRLLMAEVIDHGQGFDPNSQPQG 307 Query: 307 TGLGLYGMQERAELVGGIFSVESGIGLGTRIIIQVPV 343 TGLGLYGM ERAELV G +VE+ +G GT I ++VP+ Sbjct: 308 TGLGLYGMNERAELVNGKVNVETHLGKGTIITLEVPL 344 >Q7WZY5.1 NREB_STACT Oxygen sensor histidine kinase nreB EC=2.7.13.3 Length = 347 Score = 322 bits (826), Expect = 3e-86, Method: Compositional matrix adjust. Identities = 157/346 (45%), Positives = 228/346 (65%), Gaps = 8/346 (2%) Query: 2 DSLNTLMLKLFENSTEAIFFFDREGRTLAMNPAAAHIV--DTDFLKQLEQGNSLALCSTC 59 D L L+ + + N+ E + F + G +A+N AA + D D+ + A+C C Sbjct: 8 DKLQDLLTQYYLNTNEKMVFLNSTGEVIALNEAAEEVFADDNDYSQMTN-----AVCRRC 62 Query: 60 RGYTSDMELRTCLNCFFHVPDSEDFTSYQVYLETKNKGVIPFAASFHTIDSENGIRVLML 119 GY+++ ++ +C NCF + S+QV++ TK+ + P+ AS+ ID E GI L Sbjct: 63 EGYSNEYDIMSCENCFLEALEIGK-GSFQVFIRTKDNKIQPYTASYELIDHEKGIYAFTL 121 Query: 120 RDLTRQFKTQEKFYQNKMMKHVIEAQENERKRISRELHDSVAQELMSAVVDLRVLKYMTG 179 +++ Q + QE+ YQ KMM+ I AQENERKRISRELHD + QEL++ V+LR+LKY Sbjct: 122 HNVSPQIQRQERMYQRKMMQKTISAQENERKRISRELHDGIVQELINVDVELRLLKYQQD 181 Query: 180 DEQLLKKVKQTEVSMTRLLSDIRNLSVELRPAALDDFGLEAAFRSHFKRMEQIYGLMIEF 239 ++L+ K+ E M+RL+ D+RNLSVELRP++LDD GL+AAFRS+FK+ E+ YG+ + + Sbjct: 182 KDELIDNSKRIEGIMSRLIDDVRNLSVELRPSSLDDLGLDAAFRSYFKQFEKNYGIHVNY 241 Query: 240 ESDLSEKRYGSEIETVVYRVCQEALLNALKYAQVDLVHVKLTERDRLLKLTIEDEGVGFN 299 ++ S +R+ +EIETVVYRV QEAL NALKYAQVD+V V L + + + D GVGF Sbjct: 242 HTNFSAQRFDNEIETVVYRVVQEALFNALKYAQVDIVEVSLQLNENNIIAEVSDRGVGFK 301 Query: 300 LGDEPDGTGLGLYGMQERAELVGGIFSVESGIGLGTRIIIQVPVAD 345 GD+P GTGLGL+GM ERAELV G +++S I GT + ++VP+ D Sbjct: 302 RGDDPKGTGLGLFGMNERAELVNGTVNIDSQINRGTIVTLEVPITD 347 >Q4L8Q7.1 NREB_STAHJ Oxygen sensor histidine kinase nreB EC=2.7.13.3 Length = 344 Score = 318 bits (814), Expect = 7e-85, Method: Compositional matrix adjust. Identities = 159/340 (46%), Positives = 229/340 (67%), Gaps = 4/340 (1%) Query: 4 LNTLMLKLFENSTEAIFFFDREGRTLAMNPAAAHIVDTDFLKQLEQGNSLALCSTCRGYT 63 L L+ K +E + E I F +++G+ +AMN AA I+ D + A+C+ C GY+ Sbjct: 9 LENLLKKYYEKTNEKIVFVNKDGKVIAMNDAAEEIISKD---NNYSAMTNAICNRCEGYS 65 Query: 64 SDMELRTCLNCFFHVPDSEDFTSYQVYLETKNKGVIPFAASFHTIDSENGIRVLMLRDLT 123 ++ L++C+NC+ D ++QV+++T + + PF AS+ ID E I L+D++ Sbjct: 66 NEFALQSCINCYLDTTKPNDM-NFQVFMKTVDNKIQPFTASYQCIDDEAQIYAFTLQDIS 124 Query: 124 RQFKTQEKFYQNKMMKHVIEAQENERKRISRELHDSVAQELMSAVVDLRVLKYMTGDEQL 183 Q + QEK YQ +M++ I AQENERKRISRELHDSV QE+++ V+LR+LKY QL Sbjct: 125 PQIERQEKMYQRQMLRKTIAAQENERKRISRELHDSVVQEMLNVDVELRLLKYQQDMAQL 184 Query: 184 LKKVKQTEVSMTRLLSDIRNLSVELRPAALDDFGLEAAFRSHFKRMEQIYGLMIEFESDL 243 ++K + E+ M+ L++DIR+LSVELRP++LDD GLEAAF+S+FK++E YGL + ++S++ Sbjct: 185 IEKSEHIELLMSNLINDIRDLSVELRPSSLDDLGLEAAFKSYFKQLEYNYGLNVVYQSNI 244 Query: 244 SEKRYGSEIETVVYRVCQEALLNALKYAQVDLVHVKLTERDRLLKLTIEDEGVGFNLGDE 303 R+ SEIETVVYRV QEA+ NALKYA V V V + + + L I D G GF+ + Sbjct: 245 QTIRFDSEIETVVYRVVQEAIFNALKYAGVYEVEVTIQQTEEGLIAEIIDRGKGFDPNLK 304 Query: 304 PDGTGLGLYGMQERAELVGGIFSVESGIGLGTRIIIQVPV 343 P GTGLGLYGM ERAELV G ++E+ IG GT I ++VPV Sbjct: 305 PQGTGLGLYGMNERAELVKGTVNIETHIGKGTIITLEVPV 344 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.136 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,919,962,304 Number of extensions: 76688021 Number of successful extensions: 249878 Number of sequences better than 1.0e-05: 3381 Number of HSP's gapped: 245985 Number of HSP's successfully gapped: 3382 Length of query: 350 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 214 Effective length of database: 1,779,438,163 Effective search space: 380799766882 Effective search space used: 380799766882 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)