BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0359.0006.p (313 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KJ68.1 B3KJ68_9BACI PAS sensor protein 322 3e-86 Q5WK44.1 Q5WK44_BACSK Nitrate/nitrite transporter 311 3e-83 C0ZKM3.1 C0ZKM3_BREBN Probable nitrite extrusion protein 307 8e-82 C2XT90.1 C2XT90_BACCE Nitrite extrusion protein 301 5e-80 C3C1M4.1 C3C1M4_BACTU Nitrite extrusion protein 300 1e-79 >B3KJ68.1 B3KJ68_9BACI PAS sensor protein Length = 506 Score = 322 bits (824), Expect = 3e-86, Method: Compositional matrix adjust. Identities = 161/253 (63%), Positives = 190/253 (75%) Query: 1 MIKKLQLPLQTLNLIVGFMVWVIISSLMPFISEDISIPSGKLAMVTAIPVVLGSILRIPI 60 MIKKLQLPLQT+NL++GFMVWVIIS+L+PFI EDISIP+ ++A++TAIPVVLGS+LRIP+ Sbjct: 3 MIKKLQLPLQTMNLVLGFMVWVIISALLPFIREDISIPADRIAILTAIPVVLGSVLRIPL 62 Query: 61 GYYANIFGARIIXXXXXXXXXXXXXYISEASTYTDXXXXXXXXXXXXAVFSVGVTSLPKY 120 GYYAN+ GARI+ +IS AS++T AVFSVGVTSLPKY Sbjct: 63 GYYANVVGARIVFISSFILLLFPVYFISSASSFTHLVIGGLFLGIGGAVFSVGVTSLPKY 122 Query: 121 FPKEKHGFVNGIYGVGNLGTALTTFTAPIIAARFGWATAVKXXXXXXXXXXXXXXXXGDR 180 + KEK G VNGIYG+GN+GTA+TTF AP+IA GW+ V+ GDR Sbjct: 123 YAKEKLGLVNGIYGMGNIGTAITTFAAPVIATEIGWSKTVQLYLILLLIFALFNVFLGDR 182 Query: 181 HEPKVKTPIIEQIKGVIKNEKLWLFSLFYFITFGSFVAFTIYLPNFLVSNFGLEKVDAGM 240 E KVKTP++EQIKGV KNEKLW FSLFYFITFGSFVAFT+YLPNFLVS+F L KVDAGM Sbjct: 183 KEIKVKTPVMEQIKGVYKNEKLWFFSLFYFITFGSFVAFTVYLPNFLVSHFELAKVDAGM 242 Query: 241 RTAGFIAVATFFR 253 RTAGFI +ATF R Sbjct: 243 RTAGFIMIATFLR 255 >Q5WK44.1 Q5WK44_BACSK Nitrate/nitrite transporter Length = 502 Score = 311 bits (798), Expect = 3e-83, Method: Compositional matrix adjust. Identities = 156/253 (61%), Positives = 189/253 (74%) Query: 1 MIKKLQLPLQTLNLIVGFMVWVIISSLMPFISEDISIPSGKLAMVTAIPVVLGSILRIPI 60 MI KLQLPLQT+NL++GFMVWVIIS+L+PFI EDI+IP+ ++A++TAIPVVLGS+LRIP+ Sbjct: 1 MINKLQLPLQTMNLVLGFMVWVIISALLPFIQEDIAIPADRVAILTAIPVVLGSVLRIPL 60 Query: 61 GYYANIFGARIIXXXXXXXXXXXXXYISEASTYTDXXXXXXXXXXXXAVFSVGVTSLPKY 120 GYYAN+ GAR++ +IS A+++T AVFSVGVTSLPKY Sbjct: 61 GYYANVVGARMVFITSFILLLFPVYFISIATSFTHLVVGGLFLGIGGAVFSVGVTSLPKY 120 Query: 121 FPKEKHGFVNGIYGVGNLGTALTTFTAPIIAARFGWATAVKXXXXXXXXXXXXXXXXGDR 180 KEK G VNGIYG+GNLGTA+TTF AP+IA + GW+ V+ GDR Sbjct: 121 HLKEKLGLVNGIYGMGNLGTAITTFAAPVIATQIGWSATVQLYLILLLVFVALNAVFGDR 180 Query: 181 HEPKVKTPIIEQIKGVIKNEKLWLFSLFYFITFGSFVAFTIYLPNFLVSNFGLEKVDAGM 240 E KVKTPI+EQ+KGV KN+KLW FSLFYFITFGSFVAFT+YLPNFLVS+F L KVDAG+ Sbjct: 181 KETKVKTPIMEQVKGVYKNDKLWYFSLFYFITFGSFVAFTVYLPNFLVSHFELPKVDAGL 240 Query: 241 RTAGFIAVATFFR 253 RTAGFI +ATF R Sbjct: 241 RTAGFILIATFLR 253 >C0ZKM3.1 C0ZKM3_BREBN Probable nitrite extrusion protein Length = 499 Score = 307 bits (787), Expect = 8e-82, Method: Compositional matrix adjust. Identities = 155/253 (61%), Positives = 188/253 (74%) Query: 1 MIKKLQLPLQTLNLIVGFMVWVIISSLMPFISEDISIPSGKLAMVTAIPVVLGSILRIPI 60 M++K QLPLQTL+L VGFMVWVI+SSLMPFI E + + ++A VTAIPV+LGS+LRIP+ Sbjct: 1 MVRKWQLPLQTLSLTVGFMVWVILSSLMPFIKETVPMSPSEVAWVTAIPVLLGSLLRIPV 60 Query: 61 GYYANIFGARIIXXXXXXXXXXXXXYISEASTYTDXXXXXXXXXXXXAVFSVGVTSLPKY 120 GY++N FGAR++ YIS+A+TYTD AVFSVGVTSLPKY Sbjct: 61 GYWSNRFGARLLFSISFLLLLAPVFYISQATTYTDLLIGGLFIGIGGAVFSVGVTSLPKY 120 Query: 121 FPKEKHGFVNGIYGVGNLGTALTTFTAPIIAARFGWATAVKXXXXXXXXXXXXXXXXGDR 180 +PKEKHG VNGIYG+GN+GTA+TTF+AP++A FGWAT V+ GD+ Sbjct: 121 YPKEKHGSVNGIYGMGNIGTAITTFSAPVLAKSFGWATTVQMFMLLLVGFAVLTFLMGDK 180 Query: 181 HEPKVKTPIIEQIKGVIKNEKLWLFSLFYFITFGSFVAFTIYLPNFLVSNFGLEKVDAGM 240 +EPKV TP+ +QIK V KNEKLWL SLFYFITFG FVAFT+YLPNFLVS+F L+KVDAGM Sbjct: 181 NEPKVVTPLGQQIKSVYKNEKLWLISLFYFITFGCFVAFTVYLPNFLVSHFQLDKVDAGM 240 Query: 241 RTAGFIAVATFFR 253 RTAGFI +AT R Sbjct: 241 RTAGFILLATLAR 253 >C2XT90.1 C2XT90_BACCE Nitrite extrusion protein Length = 389 Score = 301 bits (771), Expect = 5e-80, Method: Compositional matrix adjust. Identities = 152/250 (60%), Positives = 178/250 (71%) Query: 4 KLQLPLQTLNLIVGFMVWVIISSLMPFISEDISIPSGKLAMVTAIPVVLGSILRIPIGYY 63 QL LQT NL++GFMVWVI+SSLMP+I DI + +G+++MVTA+PV+LGSILRIPIGY+ Sbjct: 5 NFQLSLQTSNLVIGFMVWVILSSLMPYIKADIPLTAGQISMVTAVPVILGSILRIPIGYW 64 Query: 64 ANIFGARIIXXXXXXXXXXXXXYISEASTYTDXXXXXXXXXXXXAVFSVGVTSLPKYFPK 123 N +GAR + YIS A++ D AVFSVGVTSLPKYFPK Sbjct: 65 TNRYGARKLFFISFILLLFPVFYISVANSMMDLIIGGLFVGIGGAVFSVGVTSLPKYFPK 124 Query: 124 EKHGFVNGIYGVGNLGTALTTFTAPIIAARFGWATAVKXXXXXXXXXXXXXXXXGDRHEP 183 E HGFVNGIYGVGN GTA+T+F AP+IA FGW T V+ GDR E Sbjct: 125 ESHGFVNGIYGVGNAGTAITSFLAPVIATSFGWRTTVQCYLILLAAFALMNFLLGDRKEK 184 Query: 184 KVKTPIIEQIKGVIKNEKLWLFSLFYFITFGSFVAFTIYLPNFLVSNFGLEKVDAGMRTA 243 KV TP+IEQIKGV KNEKLW +FYF+TFGSFVAFT+YLPNFLVS+FGLEKVDAGMRTA Sbjct: 185 KVNTPLIEQIKGVYKNEKLWFLCIFYFLTFGSFVAFTVYLPNFLVSHFGLEKVDAGMRTA 244 Query: 244 GFIAVATFFR 253 GFI +AT R Sbjct: 245 GFIVLATMMR 254 >C3C1M4.1 C3C1M4_BACTU Nitrite extrusion protein Length = 389 Score = 300 bits (768), Expect = 1e-79, Method: Compositional matrix adjust. Identities = 152/250 (60%), Positives = 178/250 (71%) Query: 4 KLQLPLQTLNLIVGFMVWVIISSLMPFISEDISIPSGKLAMVTAIPVVLGSILRIPIGYY 63 K QL LQT NLI+GFMVWVI+SSLMP+I DI + +G+++MVTA+PV+LGS+LRIPIGY+ Sbjct: 5 KFQLSLQTSNLIIGFMVWVILSSLMPYIKVDIPLTAGQISMVTAVPVILGSVLRIPIGYW 64 Query: 64 ANIFGARIIXXXXXXXXXXXXXYISEASTYTDXXXXXXXXXXXXAVFSVGVTSLPKYFPK 123 N FGAR + YIS A++ D AVFSVGVTSLPKYFPK Sbjct: 65 TNRFGARKLFFISFILLLFPVFYISVANSMMDLIIGGLFVGIGGAVFSVGVTSLPKYFPK 124 Query: 124 EKHGFVNGIYGVGNLGTALTTFTAPIIAARFGWATAVKXXXXXXXXXXXXXXXXGDRHEP 183 E HGFVNGIYGVGN GTA+T+F AP+IA GW T V+ GDR E Sbjct: 125 ESHGFVNGIYGVGNAGTAITSFLAPVIATSVGWRTTVQCYLVLLVAFALMNFLLGDRKEK 184 Query: 184 KVKTPIIEQIKGVIKNEKLWLFSLFYFITFGSFVAFTIYLPNFLVSNFGLEKVDAGMRTA 243 KV TP++EQIKGV KNEKLW +FYF+TFGSFVAFT+YLPNFLVS+FGLEKVDAGMRTA Sbjct: 185 KVNTPLMEQIKGVYKNEKLWFLCIFYFLTFGSFVAFTVYLPNFLVSHFGLEKVDAGMRTA 244 Query: 244 GFIAVATFFR 253 GFI +AT R Sbjct: 245 GFIVLATIMR 254 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.327 0.143 0.453 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,576,630,452 Number of extensions: 55547042 Number of successful extensions: 159958 Number of sequences better than 1.0e-05: 444 Number of HSP's gapped: 159784 Number of HSP's successfully gapped: 445 Length of query: 313 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 178 Effective length of database: 1,788,859,178 Effective search space: 318416933684 Effective search space used: 318416933684 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 131 (55.1 bits)