BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0359.0001.p (287 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value P96762.1 P96762_ENTGA Gentamicin resistance protein 306 1e-81 C2TDW0.1 C2TDW0_BACCE Aminoglycoside phosphotransferase 114 1e-23 A0RBJ5.1 A0RBJ5_BACAH Possible aminoglycoside phophotransferase 112 4e-23 C3EYY8.1 C3EYY8_BACTU Aminoglycoside phosphotransferase 112 4e-23 Q81T90.1 Q81T90_BACAN Aminoglycoside phophotransferase family pr... 112 5e-23 >P96762.1 P96762_ENTGA Gentamicin resistance protein Length = 306 Score = 306 bits (785), Expect = 1e-81, Method: Compositional matrix adjust. Identities = 150/246 (60%), Positives = 188/246 (76%), Gaps = 2/246 (0%) Query: 1 LKEQERRYSALIADQFPELNIRTVESLGEGYRNFAVLVNGEWVFRFPKSQQGADELNKEM 60 +K+ + Y+ +I QFP+++I++VESLGEG+RN+A+LVNG+WVFRFPKSQQGADELNKE+ Sbjct: 1 MKQNKLHYTTMIMTQFPDISIQSVESLGEGFRNYAILVNGDWVFRFPKSQQGADELNKEI 60 Query: 61 HLLPLLSERVKVGIPKFVYRGTQEDGRLFAGYRKVEGEILGEDGIASVSGDARERLAIQL 120 LLPLL VKV IP++VY G + DG F GYRKV+G+ILGEDG+A DA++RLA+QL Sbjct: 61 QLLPLLVGCVKVNIPQYVYIGKRSDGNPFVGYRKVQGQILGEDGMAVFPDDAKDRLALQL 120 Query: 121 GDFMSDLNAFPVETAIQAGVPVQQLADEIQLLKDAAERQVFPHLEPSSRGYLHRRFQAYL 180 +FM++L+AFPVETAI AGVPV L ++I LL +A E QVFP L+ S R YL RFQ+Y+ Sbjct: 121 AEFMNELSAFPVETAISAGVPVTNLKNKILLLSEAVEDQVFPLLDESLRDYLTLRFQSYM 180 Query: 181 ENTEYPRYIPALIHADLSPDHFLT--DSHRSVLTGIIDFGDAAISXXXXXXXXXXEDCGE 238 + Y RY P LIH DLSPDHFLT +S ++ LTGIIDFGDAAIS EDCGE Sbjct: 181 THPVYTRYTPRLIHGDLSPDHFLTNLNSRQTPLTGIIDFGDAAISDPDYDYVYLLEDCGE 240 Query: 239 PFTRQV 244 FTRQV Sbjct: 241 LFTRQV 246 >C2TDW0.1 C2TDW0_BACCE Aminoglycoside phosphotransferase Length = 300 Score = 114 bits (284), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 67/240 (27%), Positives = 119/240 (49%), Gaps = 3/240 (1%) Query: 8 YSALIADQFPELNIRTVESLGEGYRNFAVLVNGEWVFRFPKSQQGADELNKEMHLLPLLS 67 Y I + P L+I + + EG+ N AV+VN E +FRFP+ Q+ A + E L +L+ Sbjct: 4 YKQYIKEALPNLSIHSYKQNEEGWDNVAVIVNDELLFRFPRKQEYAMRIPLEKELCTILT 63 Query: 68 ERVK-VGIPKF--VYRGTQEDGRLFAGYRKVEGEILGEDGIASVSGDARERLAIQLGDFM 124 + ++ + +P++ +Y+ ++ L + Y + GE L + +A++ R+ + QL F+ Sbjct: 64 QSLQEIEVPQYHLIYKNESDEVPLCSYYTLIHGEPLKTEIVANLDEKERKIIITQLATFL 123 Query: 125 SDLNAFPVETAIQAGVPVQQLADEIQLLKDAAERQVFPHLEPSSRGYLHRRFQAYLENTE 184 + L++ P+++ I G P ++ + L+ V L + L+R F+ + Sbjct: 124 AALHSIPLKSVIALGFPTEKTLTYWKELQTKLNEYVTNSLTSFQKSTLNRLFENFFACIA 183 Query: 185 YPRYIPALIHADLSPDHFLTDSHRSVLTGIIDFGDAAISXXXXXXXXXXEDCGEPFTRQV 244 + A+IHAD + H L D +++GIIDFGDA I D G FT V Sbjct: 184 TSAFPNAIIHADFTHHHILFDKQNKIISGIIDFGDAQIGDPAFDFAGLYYDFGHEFTTSV 243 >A0RBJ5.1 A0RBJ5_BACAH Possible aminoglycoside phophotransferase Length = 300 Score = 112 bits (280), Expect = 4e-23, Method: Compositional matrix adjust. Identities = 66/240 (27%), Positives = 118/240 (49%), Gaps = 3/240 (1%) Query: 8 YSALIADQFPELNIRTVESLGEGYRNFAVLVNGEWVFRFPKSQQGADELNKEMHLLPLLS 67 Y I + P L+I + + EG+ N AV+VN E +FRFP+ Q+ A + E L +L+ Sbjct: 4 YKQYIKEALPNLSIHSYKQNEEGWDNVAVIVNDELLFRFPRKQEYAMRIPLEKELCTILT 63 Query: 68 ERVK-VGIPKF--VYRGTQEDGRLFAGYRKVEGEILGEDGIASVSGDARERLAIQLGDFM 124 + ++ + +P++ +Y+ ++ L + Y + GE L + +A++ R+ + QL F+ Sbjct: 64 QSLQEIEVPQYHLIYKNESDEVPLCSYYTLIHGEPLKTEIVANLDEKERKIIITQLATFL 123 Query: 125 SDLNAFPVETAIQAGVPVQQLADEIQLLKDAAERQVFPHLEPSSRGYLHRRFQAYLENTE 184 + L++ P+++ G P ++ + L+ V L + L+R F+ + Sbjct: 124 AALHSIPLQSVTALGFPTEKTLTYWKELQTKLNEYVTNSLTSFQKSTLNRLFENFFACIA 183 Query: 185 YPRYIPALIHADLSPDHFLTDSHRSVLTGIIDFGDAAISXXXXXXXXXXEDCGEPFTRQV 244 + A+IHAD + H L D +++GIIDFGDA I D G FT V Sbjct: 184 TSAFPNAIIHADFTHHHILFDKQNKIISGIIDFGDAQIGDPAFDFAGLYYDFGHEFTTSV 243 >C3EYY8.1 C3EYY8_BACTU Aminoglycoside phosphotransferase Length = 300 Score = 112 bits (280), Expect = 4e-23, Method: Compositional matrix adjust. Identities = 66/240 (27%), Positives = 118/240 (49%), Gaps = 3/240 (1%) Query: 8 YSALIADQFPELNIRTVESLGEGYRNFAVLVNGEWVFRFPKSQQGADELNKEMHLLPLLS 67 Y I + P L+I + + EG+ N AV+VN E +FRFP+ Q+ A + E L +L+ Sbjct: 4 YKQYIKEALPNLSIHSYKQNEEGWDNVAVIVNDELLFRFPRKQEYAMRIPLEKELCTILT 63 Query: 68 ERVK-VGIPKF--VYRGTQEDGRLFAGYRKVEGEILGEDGIASVSGDARERLAIQLGDFM 124 + ++ + +P++ +Y+ ++ L + Y + GE L + +A++ R+ + QL F+ Sbjct: 64 QSLQEIEVPQYHLIYKNESDEVPLCSYYTLIHGEPLKTEIVANLDEKERKIIITQLATFL 123 Query: 125 SDLNAFPVETAIQAGVPVQQLADEIQLLKDAAERQVFPHLEPSSRGYLHRRFQAYLENTE 184 + L++ P+++ G P ++ + L+ V L + L+R F+ + Sbjct: 124 AALHSIPLKSVTALGFPTEKTLTYWKELQTKLNEYVTNSLTSFQKSTLNRLFENFFSCIA 183 Query: 185 YPRYIPALIHADLSPDHFLTDSHRSVLTGIIDFGDAAISXXXXXXXXXXEDCGEPFTRQV 244 + A+IHAD + H L D +++GIIDFGDA I D G FT V Sbjct: 184 TSAFPNAIIHADFTHHHILFDKQNKIISGIIDFGDAQIGDPAFDFAGLYYDFGHEFTTSV 243 >Q81T90.1 Q81T90_BACAN Aminoglycoside phophotransferase family protein Length = 300 Score = 112 bits (279), Expect = 5e-23, Method: Compositional matrix adjust. Identities = 66/240 (27%), Positives = 118/240 (49%), Gaps = 3/240 (1%) Query: 8 YSALIADQFPELNIRTVESLGEGYRNFAVLVNGEWVFRFPKSQQGADELNKEMHLLPLLS 67 Y I + P L+I + + EG+ N AV+VN E +FRFP+ Q+ A + E L +L+ Sbjct: 4 YKQYIKEALPNLSIHSYKQNEEGWDNVAVIVNDELLFRFPRKQEYAMRIPLEKELCTILT 63 Query: 68 ERVK-VGIPKF--VYRGTQEDGRLFAGYRKVEGEILGEDGIASVSGDARERLAIQLGDFM 124 + ++ + +P++ +Y+ ++ L + Y + GE L + +A++ R+ + QL F+ Sbjct: 64 QSLQEIEVPQYHLIYKNESDEVPLCSYYTLIHGEPLKTEIVANLDEKERKIIITQLATFL 123 Query: 125 SDLNAFPVETAIQAGVPVQQLADEIQLLKDAAERQVFPHLEPSSRGYLHRRFQAYLENTE 184 + L++ P+++ G P ++ + L+ V L + L+R F+ + Sbjct: 124 AALHSIPLKSVTALGFPTEKTLTYWKELQTKLNEYVTNSLTSFQKSTLNRLFENFFACIA 183 Query: 185 YPRYIPALIHADLSPDHFLTDSHRSVLTGIIDFGDAAISXXXXXXXXXXEDCGEPFTRQV 244 + A+IHAD + H L D +++GIIDFGDA I D G FT V Sbjct: 184 TSAFPNAIIHADFTHHHILFDKQNKIISGIIDFGDAQIGDPAFDFAGLYYDFGHEFTTSV 243 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.140 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,608,668,719 Number of extensions: 65100557 Number of successful extensions: 144532 Number of sequences better than 1.0e-05: 127 Number of HSP's gapped: 144405 Number of HSP's successfully gapped: 127 Length of query: 287 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 153 Effective length of database: 1,798,280,193 Effective search space: 275136869529 Effective search space used: 275136869529 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)