BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0352.0014.p (432 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A4J2V1.1 A4J2V1_DESRM Transposase, IS204/IS1001/IS1096/IS1165 fa... 499 e-139 Q65S07.1 Q65S07_MANSM Putative uncharacterized protein 479 e-133 Q6V4T5.1 Q6V4T5_ENTFC TnpA 450 e-124 B2SSB8.1 B2SSB8_XANOP Transposase TnpA, ISL3 family 449 e-124 Q3D1N8.1 Q3D1N8_STRAG Transposase, ISL3 family 446 e-123 >A4J2V1.1 A4J2V1_DESRM Transposase, IS204/IS1001/IS1096/IS1165 family protein Length = 434 Score = 499 bits (1286), Expect = e-139, Method: Compositional matrix adjust. Identities = 238/436 (54%), Positives = 323/436 (74%), Gaps = 6/436 (1%) Query: 1 MDILNLSKFNILSISENEFDFLIQVVTSSPPLACPHCGCIANLYKHDSREQICMDLPIHG 60 MDILNL F +L + + E D+ I+ +SPP+ CP+C + + +EQ+ MDLPIHG Sbjct: 1 MDILNLPHFKVLHVEQTEHDYSIRAEMASPPIICPNCYN-RDFVGYGKKEQLFMDLPIHG 59 Query: 61 KRVGLLIKRQRYRCRDCNRTFWEHLDHTIDEKRNCTKRLLSYIEKQSLQRTFVSISEDVG 120 KRVG+L+ R+RYRC++CN+TF E L +DEKR T RL+ Y++KQSL+RTF SISEDVG Sbjct: 60 KRVGILVNRRRYRCKECNKTFLEILP-DMDEKRLATTRLIEYVQKQSLKRTFASISEDVG 118 Query: 121 LHEKTIRNIFRDYINRLKETLRFETPNWLGIDEIHIIKPRCVLTNIEERTLLDVLPNRNK 180 L EKT+RNIFRDY+ L++T+RF TP +LGIDEIHII PRCV+ N+++RT++++L NRNK Sbjct: 119 LDEKTVRNIFRDYVTHLEKTVRFATPEYLGIDEIHIIHPRCVVCNVKDRTIINILQNRNK 178 Query: 181 ETVVGYLSGLPNRGEIQYVTMNMWQPYKDAVRAILPKALIIVDKFHVVRMANQALETIRK 240 ETV+ YL LP+R ++YV+M+MWQPY+DAV+ +LP+A IIVDKFHV+RMANQ +ET+RK Sbjct: 179 ETVIKYLMELPDRDRVKYVSMDMWQPYRDAVKLVLPQAKIIVDKFHVLRMANQGVETVRK 238 Query: 241 QLREGLSPKERRGLMHDRFILLKRHTALTEMDKITLDLWTKNHPSLGTAYDLKESFFDIW 300 +R ++PK+RR LMHDRF+LLKR L E + + L+ WTKN P LG AY+LKE FF ++ Sbjct: 239 DIRANMTPKQRRTLMHDRFVLLKRRHELKEAEFLKLESWTKNLPDLGKAYELKEEFFQVY 298 Query: 301 ECDSRQTAFLKYNEWKAKIPTELQSAFEPLTKAMKNWEEEIFAYFDHRITNAYMESLNSL 360 E ++ A +YN W +K+ E+ AFEPL KA+ NW E+F YF+H ITNAY E+LN L Sbjct: 299 ESKNQAEAIERYNAWLSKVTDEICFAFEPLIKALTNWHHEVFNYFNHPITNAYTEALNGL 358 Query: 361 IRVINRLGRGYSFEALRAKILFTEGLKKQRKP----EENFPMFNLPPIGIVREPEEIILP 416 IRV+NRLGRGYSFEALRAKILFTEGL K ++P + P+ + I + Sbjct: 359 IRVMNRLGRGYSFEALRAKILFTEGLSKSKRPLYRRQNREPIMSRMVIAETTIDYQNWDT 418 Query: 417 GIDFSTLIEKLEKGQL 432 G+ +TL++ +EKG++ Sbjct: 419 GVPITTLVDFIEKGKI 434 >Q65S07.1 Q65S07_MANSM Putative uncharacterized protein Length = 436 Score = 479 bits (1232), Expect = e-133, Method: Compositional matrix adjust. Identities = 227/437 (51%), Positives = 310/437 (70%), Gaps = 9/437 (2%) Query: 2 DILNLSKFNILSISENEFDFLIQVVTSSPPLACPHCGCIANLYKHDSREQICMDLPIHGK 61 +ILN + +L +SE E D+ + S PP CPHC + R+++ MD P+HG+ Sbjct: 3 NILNWPDYKVLQVSELEHDYQVHAEVSEPPTQCPHCNH-PEIVGFGRRDEVIMDTPVHGR 61 Query: 62 RVGLLIKRQRYRCRDCNRTFWEHLDHTIDEKRNCTKRLLSYIEKQSLQRTFVSISEDVGL 121 R G+++ R+RYRC+ C +TF E + H DEKR T RL+ YIE++SL+RTF S++EDVG+ Sbjct: 62 RTGIMLNRRRYRCQSCRKTFLEPVPHK-DEKRQMTNRLIQYIERESLRRTFSSVAEDVGV 120 Query: 122 HEKTIRNIFRDYINRLKETLRFETPNWLGIDEIHIIKPRCVLTNIEERTLLDVLPNRNKE 181 EKT+RNIF DY RL++TL FE P WLGIDEIHIIKPRCV+TNI+++T++D+L NRNK Sbjct: 121 DEKTVRNIFNDYCERLEKTLNFEMPQWLGIDEIHIIKPRCVITNIQQQTIVDMLDNRNKT 180 Query: 182 TVVGYLSGLPNRGEIQYVTMNMWQPYKDAVRAILPKALIIVDKFHVVRMANQALETIRKQ 241 TV YLS +R ++YV M+MW+PY+ AV ++P A +I+DKFHVVRMAN++LE RK Sbjct: 181 TVTRYLSKRTDRDLVRYVAMDMWRPYRQAVETMIPDATVIIDKFHVVRMANESLERARKA 240 Query: 242 LREGLSPKERRGLMHDRFILLKRHTALTEMDKITLDLWTKNHPSLGTAYDLKESFFDIWE 301 +R L+P++RRGLM DRF+LLKR LT+ + + WT N+P +G AY+LKE+FF+IW+ Sbjct: 241 IRSALTPQQRRGLMRDRFVLLKRRHELTDAEYMRFSGWTLNYPEIGQAYELKEAFFEIWD 300 Query: 302 CDSRQTAFLKYNEWKAKIPTELQSAFEPLTKAMKNWEEEIFAYFDHRITNAYMESLNSLI 361 C +R A Y W +I E+++ ++PL KAM NW ++IFAYFDH ITNAY ESLN+LI Sbjct: 301 CQTRHQAQEAYYSWLRQITPEMKAHYDPLIKAMGNWHDDIFAYFDHPITNAYTESLNNLI 360 Query: 362 RVINRLGRGYSFEALRAKILFTEGLKKQRKP---EENFP---MFNLPPIGIVREPEEIIL 415 RV+NR+GRGYSFEALRAKILFTEG +K +KP + P M +P G+ E Sbjct: 361 RVVNRVGRGYSFEALRAKILFTEGFQKIKKPRYQRQRIPEGAMGRMPFYGVA-EAGPSTN 419 Query: 416 PGIDFSTLIEKLEKGQL 432 G D STL+ ++E G+L Sbjct: 420 YGADISTLVREIEAGRL 436 >Q6V4T5.1 Q6V4T5_ENTFC TnpA Length = 440 Score = 450 bits (1157), Expect = e-124, Method: Compositional matrix adjust. Identities = 232/440 (52%), Positives = 302/440 (68%), Gaps = 11/440 (2%) Query: 2 DILNLSKF-NILSISENEFDFLIQVVTSSPPLACPHCGCIANLYKHDSREQICMDLPIHG 60 D+L+L I S ENE D + +V PP CP CG LYKH SR Q+ MDLPI Sbjct: 3 DLLSLPDIKTIESPQENETDMMFKVEAVGPPERCPECG-FDKLYKHSSRNQLIMDLPIRL 61 Query: 61 KRVGLLIKRQRYRCRDCNRTFWEHLDHTIDEKRNCTKRLLSYIEKQSLQRTFVSISEDVG 120 KRVGL + R+RY+CR+C TFWE L ++DEKR+ TKRLL I++QS+ +TFV ++E VG Sbjct: 62 KRVGLQLNRRRYKCRECGSTFWERL-VSVDEKRSMTKRLLKSIQEQSMSKTFVEVAESVG 120 Query: 121 LHEKTIRNIFRDYINRLKETLRFETPNWLGIDEIHIIK-PRCVLTNIEERTLLDVLPNRN 179 + EKTIRN+F+DY+ + +FETP WLGIDEIHII+ PR VLTNIE RT+ D+ PNRN Sbjct: 121 VDEKTIRNVFKDYVALKEREYQFETPKWLGIDEIHIIRRPRLVLTNIERRTIYDIKPNRN 180 Query: 180 KETVVGYLSGLPNRGEIQYVTMNMWQPYKDAVRAILPKALIIVDKFHVVRMANQALETIR 239 KETV+ LS + +R I+YVTM+MW+PYKDAV ILP A ++VDKFHVVRMANQAL+ +R Sbjct: 181 KETVIQRLSEISDRTYIEYVTMDMWKPYKDAVNTILPHAKVVVDKFHVVRMANQALDNVR 240 Query: 240 KQLREGLSPKERRGLMHDRFILLKRHTALTEMDKITLDLWTKNHPSLGTAYDLKESFFDI 299 K L+ +S KERR LM +RFILLKR L E + L+ W N P+L AY+LKE F+ I Sbjct: 241 KSLKAHMSQKERRTLMRERFILLKRKHDLNERESFLLETWLGNLPALKEAYELKEEFYWI 300 Query: 300 WECDSRQTAFLKYNEWKAK-IPTELQSAFEPLTKAMKNWEEEIFAYFDHRITNAYMESLN 358 W+ + L+Y++W+ + + + + A++ L +A+ NW EIF YFD R+TNAY ES+N Sbjct: 301 WDTPNSDEGRLRYSQWRHRCMSSNSKDAYKDLVRAVDNWHVEIFNYFDKRLTNAYTESIN 360 Query: 359 SLIRVINRLGRGYSFEALRAKILFTEGLKKQRKPEENFPMFNLPPIGI------VREPEE 412 S+IR + R+GRGYSF+ALRAKILF E L K+RKP N FN + V + Sbjct: 361 SIIRQVERMGRGYSFDALRAKILFNEKLHKKRKPRFNSSAFNKAMLHDNFNWYEVNDHNI 420 Query: 413 IILPGIDFSTLIEKLEKGQL 432 G+DFSTLI+ LEKG L Sbjct: 421 TDNFGVDFSTLIKNLEKGDL 440 >B2SSB8.1 B2SSB8_XANOP Transposase TnpA, ISL3 family Length = 472 Score = 449 bits (1156), Expect = e-124, Method: Compositional matrix adjust. Identities = 215/416 (51%), Positives = 283/416 (68%), Gaps = 3/416 (0%) Query: 2 DILNLSKFNILSISENEFDFLIQVVTSSPPLACPHCGCIANLYKHDSREQICMDLPIHGK 61 +ILNL F + + E + D+ + S+PP C CG L H EQ+ DLP HGK Sbjct: 3 NILNLPDFKVQRVEEADHDYHVYAEVSNPPGVCTACGS-DRLIGHGRNEQVVRDLPTHGK 61 Query: 62 RVGLLIKRQRYRCRDCNRTFWEHLDHTIDEKRNCTKRLLSYIEKQSLQRTFVSISEDVGL 121 R+ + + +R+RC+ C +TF E L ++ KR T RL+ +I +QSL+RTF SI++D GL Sbjct: 62 RLAIYVDTRRWRCQSCGKTFMEPLP-AVNAKREMTDRLVKWIGQQSLKRTFASIADDTGL 120 Query: 122 HEKTIRNIFRDYINRLKETLRFETPNWLGIDEIHIIKPRCVLTNIEERTLLDVLPNRNKE 181 EKTIRNIFRDYIN L+ RFETP W+GIDEIH+I+PRCV++NI T++D+LPNR+K+ Sbjct: 121 DEKTIRNIFRDYINELEAEFRFETPKWMGIDEIHLIRPRCVISNIRNNTIIDMLPNRDKK 180 Query: 182 TVVGYLSGLPNRGEIQYVTMNMWQPYKDAVRAILPKALIIVDKFHVVRMANQALETIRKQ 241 TVV YL L + E+QYV M+MW PY+DAV+A++P A I++DKFHVVRMAN A+E +RK Sbjct: 181 TVVNYLYNLQGKSEVQYVAMDMWTPYRDAVQAVIPDARIVIDKFHVVRMANDAVEKVRKS 240 Query: 242 LREGLSPKERRGLMHDRFILLKRHTALTEMDKITLDLWTKNHPSLGTAYDLKESFFDIWE 301 LRE L+PK+RRGLMHDRF+LLKR L + + + LD WTKN+P LG AY LKE F+ ++E Sbjct: 241 LREQLTPKQRRGLMHDRFVLLKRERDLNDKEALLLDGWTKNYPELGAAYRLKEDFYAVYE 300 Query: 302 CD-SRQTAFLKYNEWKAKIPTELQSAFEPLTKAMKNWEEEIFAYFDHRITNAYMESLNSL 360 S + A Y W + E++ AF L +A NW+ I YF+H +TNAY ESLNSL Sbjct: 301 GSASPEAALAAYEAWNKAVVPEVRDAFGDLIRAFTNWQPWIINYFEHPVTNAYTESLNSL 360 Query: 361 IRVINRLGRGYSFEALRAKILFTEGLKKQRKPEENFPMFNLPPIGIVREPEEIILP 416 IRV+NRLGRGYSFEALRAKILFTEG K + F P + + E +P Sbjct: 361 IRVMNRLGRGYSFEALRAKILFTEGAHKHKLSRPKFERRREPDLAMAEEVAGYGVP 416 >Q3D1N8.1 Q3D1N8_STRAG Transposase, ISL3 family Length = 440 Score = 446 bits (1146), Expect = e-123, Method: Compositional matrix adjust. Identities = 226/425 (53%), Positives = 295/425 (69%), Gaps = 10/425 (2%) Query: 16 ENEFDFLIQVVTSSPPLACPHCGCIANLYKHDSREQICMDLPIHGKRVGLLIKRQRYRCR 75 ENE D + +V PP CP CG LYKH SR Q+ MDLPI KRVGL + R+RY+CR Sbjct: 18 ENETDMMFKVEAVGPPERCPECG-FDKLYKHSSRNQLIMDLPIRLKRVGLHLNRRRYKCR 76 Query: 76 DCNRTFWEHLDHTIDEKRNCTKRLLSYIEKQSLQRTFVSISEDVGLHEKTIRNIFRDYIN 135 +C TFWE L ++DEKR+ TKRLL I++QS+ +TFV ++E VG+ EKTIRN+F+DY+ Sbjct: 77 ECGSTFWERL-ISVDEKRSMTKRLLKSIQEQSMSKTFVEVAESVGVDEKTIRNVFKDYVA 135 Query: 136 RLKETLRFETPNWLGIDEIHIIK-PRCVLTNIEERTLLDVLPNRNKETVVGYLSGLPNRG 194 + +FETP WLGIDEIHII+ PR VLTNIE RT+ D+ PNRNKETV+ LS + +R Sbjct: 136 LKEREYQFETPKWLGIDEIHIIRRPRLVLTNIERRTIYDIKPNRNKETVIQRLSEISDRT 195 Query: 195 EIQYVTMNMWQPYKDAVRAILPKALIIVDKFHVVRMANQALETIRKQLREGLSPKERRGL 254 I+YVTM+MW+PYKDAV ILP+A ++VDKFHVVRMA+QAL+ +RK L+ +S KERR L Sbjct: 196 YIEYVTMDMWKPYKDAVNTILPQAKVVVDKFHVVRMAHQALDNVRKSLKAHMSQKERRTL 255 Query: 255 MHDRFILLKRHTALTEMDKITLDLWTKNHPSLGTAYDLKESFFDIWECDSRQTAFLKYNE 314 M +RFILLKR L E + LD W N P+L AY+LKE F+ IW+ L+Y++ Sbjct: 256 MRERFILLKRKHDLNERESFLLDTWLGNLPALKEAYELKEEFYWIWDTPDPDEGHLRYSQ 315 Query: 315 WKAK-IPTELQSAFEPLTKAMKNWEEEIFAYFDHRITNAYMESLNSLIRVINRLGRGYSF 373 W+ + + + + A++ +A+ NW EIF YFD R+TNAY ES+NS+IR + R+GRGYSF Sbjct: 316 WRHRCMSSNSKDAYKDXVRAVDNWHVEIFNYFDKRLTNAYTESINSIIRQVERMGRGYSF 375 Query: 374 EALRAKILFTEGLKKQRKPEENFPMFNLPPIGI------VREPEEIILPGIDFSTLIEKL 427 +ALRAKILF E L K+RKP N FN + V + + G+DFSTLI+ L Sbjct: 376 DALRAKILFNEKLHKKRKPRFNSSAFNKAMLYDTFNWYEVNDHDITDNLGVDFSTLIKNL 435 Query: 428 EKGQL 432 EKG L Sbjct: 436 EKGDL 440 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.323 0.139 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,548,068,296 Number of extensions: 105048283 Number of successful extensions: 282332 Number of sequences better than 1.0e-05: 978 Number of HSP's gapped: 280892 Number of HSP's successfully gapped: 978 Length of query: 432 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 293 Effective length of database: 1,751,175,118 Effective search space: 513094309574 Effective search space used: 513094309574 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)