BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0352.0006.p (303 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K5E1.1 B3K5E1_9BACI Pectinesterase EC=3.1.1.11 353 8e-96 Q97DU8.1 Q97DU8_CLOAB Pectin methylesterase 268 4e-70 B1ZP19.1 B1ZP19_OPITP Pectinesterase EC=3.1.1.11 266 1e-69 A8FE12.1 A8FE12_BACP2 Pectinesterase EC=3.1.1.11 263 2e-68 B4AL30.1 B4AL30_BACPU Pectinesterase 259 3e-67 >B3K5E1.1 B3K5E1_9BACI Pectinesterase EC=3.1.1.11 Length = 308 Score = 353 bits (907), Expect = 8e-96, Method: Compositional matrix adjust. Identities = 169/294 (57%), Positives = 212/294 (72%), Gaps = 3/294 (1%) Query: 8 MLITVSKSVGEGFSSLQAALDSIPQEYKEAVTIRIKPGIYEEKVTVKQGTPPVLLLGEDT 67 M ITV S F ++Q+A+DSIP++ V + IK G+Y EK+T+ P + ++GE Sbjct: 1 MKITVDPSGQGDFVTVQSAVDSIPEQADSLVILEIKKGVYCEKITIPSSKPTIRMIGEGA 60 Query: 68 NSTIITWSDNAHTLGPDGEPLGTFKSGTLNVFAEHFTAENLTIRNASGPGTGQAVAAFVD 127 TI+T+SDNAHTLG DG+PLGTF+SG+L V+A+ F+AE LT+RN SGPGTGQAVAAF+D Sbjct: 61 EETILTYSDNAHTLGEDGQPLGTFRSGSLYVYADDFSAEQLTVRNDSGPGTGQAVAAFID 120 Query: 128 AGHAVFRRVRLLGDQDTLYTGQGKQYYNECYIEGDVDYIFGAATALFERCRLHNKRSRGY 187 A F+ VRL DQDTLY G+ Y+ EC+IEGDVD+IFG A A+F+RC + KRS GY Sbjct: 121 ADRVSFQHVRLEDDQDTLYVSGGRHYFAECFIEGDVDFIFGPAAAVFDRCMIRCKRSGGY 180 Query: 188 ITAASTPEGAPFGYVFLDCEITSGEGVSEVYLGRPWRPYAHVAFIRTVMDGSIIGEGWHN 247 +TAA+TP+ A FGYVFLDC I+ GV VYLGRPWR YA+V FIR MDGS+ +GWHN Sbjct: 181 LTAANTPKEAEFGYVFLDCTISGAPGVENVYLGRPWRDYANVVFIRCEMDGSVHPQGWHN 240 Query: 248 WGQPDREETSRYEEYGSSGPGANPEARAAWSRQLIPQEAAQY---RVLSVLDGW 298 W QPDRE+TSRY E+ S GPGA P R +WSR L EA + +VLS DGW Sbjct: 241 WNQPDREQTSRYAEFDSRGPGAAPSLRVSWSRDLTEAEAKPFTIEQVLSGQDGW 294 >Q97DU8.1 Q97DU8_CLOAB Pectin methylesterase Length = 321 Score = 268 bits (685), Expect = 4e-70, Method: Compositional matrix adjust. Identities = 134/316 (42%), Positives = 191/316 (60%), Gaps = 30/316 (9%) Query: 10 ITVSKSVGEGFSSLQAALDSIPQEYKEAVTIRIKPGIYEEKVTVKQGTPPVLLLGEDTNS 69 + VSK+ F ++Q A+DS+ + K+ V I++K G+Y+EK+++++ P + L+GED +S Sbjct: 1 MIVSKNDDGDFDTIQKAVDSVSKNNKKRVIIKVKAGVYKEKLSIRK--PFISLIGEDVSS 58 Query: 70 TIITWSDNAHTLGPDGEPLGTFKSGTLNVFAEHFTAENLTIRNASGPG--TGQAVAAFVD 127 T+IT++D+A+TL + E + TF S T+ V + F EN+T+ N +G G GQAVA + D Sbjct: 59 TVITFNDSANTLMANKERMRTFNSYTMFVDGDDFICENITVENNAGDGDLVGQAVAVYAD 118 Query: 128 AGHAVFRRVRLLGDQDTLYTG----------------------QGKQYYNECYIEGDVDY 165 +FR RLL +QDTL+TG +QYY CYI GD+D+ Sbjct: 119 GDRMIFRNCRLLANQDTLFTGPLPPKPIEGNNFGGPKDGMKRRDVRQYYENCYIRGDIDF 178 Query: 166 IFGAATALFERCRL----HNKRSRGYITAASTPEGAPFGYVFLDCEITSGEGVSEVYLGR 221 IFG+ATA+F +C + NK G+I AASTPEG FGYVFLDC+ S VYLGR Sbjct: 179 IFGSATAVFNKCEIFSNDKNKEVNGFIAAASTPEGKEFGYVFLDCKFISDARKHTVYLGR 238 Query: 222 PWRPYAHVAFIRTVMDGSIIGEGWHNWGQPDREETSRYEEYGSSGPGANPEARAAWSRQL 281 PWR YA FIR M II EG+HNW + + E+ S Y EY S GPGA + R W++ L Sbjct: 239 PWRDYAKTVFIRCFMGEHIIPEGFHNWNKANAEKESYYAEYKSYGPGAANDKRVKWAKLL 298 Query: 282 IPQEAAQYRVLSVLDG 297 +E +Y + ++L G Sbjct: 299 NDKEVEKYSITNILKG 314 >B1ZP19.1 B1ZP19_OPITP Pectinesterase EC=3.1.1.11 Length = 571 Score = 266 bits (681), Expect = 1e-69, Method: Compositional matrix adjust. Identities = 136/287 (47%), Positives = 184/287 (64%), Gaps = 9/287 (3%) Query: 20 FSSLQAALDSIPQEYKEAV---TIRIKPGIYEEKVTVKQGTPPVLLLGEDTNSTIITWSD 76 F+SLQ A+ + P A I +KPG Y E++ V++ + +LGED +TI+++ Sbjct: 38 FTSLQDAISAAPMRTDPAAPAWVILVKPGTYRERIYVQRERGNIHVLGEDATTTIVSYDL 97 Query: 77 NAHTLGPDGEPLGTFKSGTLNVFAEHFTAENLTIRNASGPGTGQAVAAFVDAGHAVFRRV 136 +A+ GPDG+P+GTF++ TL + + EN+TI N++GP GQA+A D VFR Sbjct: 98 HANLPGPDGKPIGTFRTPTLQIDGDGMIWENITIANSAGP-VGQALALRADGDRLVFRHC 156 Query: 137 RLLGDQDTLYTGQGKQYYNECYIEGDVDYIFGAATALFERCRLHNKRSRGYITAASTPEG 196 R LG QDTL +G+ Y+ +C IEG VD+IFGAATA F+ C + R GYITAASTP+G Sbjct: 157 RFLGWQDTLLLNRGRHYFVDCTIEGHVDFIFGAATAFFDHCAIRCLRD-GYITAASTPKG 215 Query: 197 APFGYVFLDCEITSGEGVSEVYLGRPWRPYAHVAFIRTVMDGSIIGEGWHNWGQPDREET 256 A G+VF DC IT EGV + YLGRPWR +A F+RT M ++ EGWHNW +P E+T Sbjct: 216 AAHGFVFADCTITGAEGV-KTYLGRPWRDFAQTVFLRTEMSAAVRPEGWHNWNKPHAEQT 274 Query: 257 SRYEEYGSSGPGANPEARAAWSRQLIPQEAAQYRVLSVL---DGWHP 300 + Y E+GS+GPGANP AR AW+ L ++AA VL DGW P Sbjct: 275 TFYAEFGSTGPGANPSARVAWAHTLTAEDAADLTPAHVLGGADGWDP 321 >A8FE12.1 A8FE12_BACP2 Pectinesterase EC=3.1.1.11 Length = 326 Score = 263 bits (671), Expect = 2e-68, Method: Compositional matrix adjust. Identities = 136/302 (45%), Positives = 190/302 (62%), Gaps = 12/302 (3%) Query: 5 EPQMLITVSKSVGEGFSSLQAALDSIPQEYKEAVTIRIKPGIYEEKVTVKQGTPPVLLLG 64 + ++ V + F ++Q+A+D+IP ++ VTI IK G+Y+EK+ + Q P V L+G Sbjct: 30 QTNRVLVVDQKGNGSFRTVQSAIDAIPANNQQRVTIYIKNGVYKEKILLPQNKPYVSLIG 89 Query: 65 EDTNSTIITWSDNAHTLGPDGEPLGTFKSGTLNVFAEHFTAENLTIRNASGPGTGQAVAA 124 ED ++TI+T++D + T S + + A F AEN+T +N +G GQAVA Sbjct: 90 EDQDNTILTYND------TNASTGSTTNSSSTMIRANDFYAENITFQNTAGRYAGQAVAL 143 Query: 125 FVDAGHAVFRRVRLLGDQDTLY-TGQGKQYYNECYIEGDVDYIFGAATALFERCRLHNKR 183 +V A F+++R+LG QDTLY TG G+QYY CYIEG VD+IFG+ATA+F+R + + Sbjct: 144 YVSGDRATFKQIRVLGYQDTLYATGTGRQYYENCYIEGTVDFIFGSATAVFKRAEIKS-L 202 Query: 184 SRGYITAASTPEGAPFGYVFLDCEITSGEGVSE-VYLGRPWRPYAHVAFIRTVMDGSIIG 242 GYITAAST E +GYV +D + G ++ VYLGRPWRP++ V F+ T MD I Sbjct: 203 GNGYITAASTTESQKYGYVLIDSTLQKGTAAAQSVYLGRPWRPHSAVTFLNTKMDHHIKA 262 Query: 243 EGWHNWGQPDREETSRYEEYGSSGPGANPEARAAWSRQLIPQEAAQYRVLSVL---DGWH 299 EGWHNW D E T+RY+EYGS+G G+N R WS L EA+Q V +VL DGW+ Sbjct: 263 EGWHNWDNRDNERTARYKEYGSTGAGSNLTNRVKWSSILTKNEASQITVQAVLGGADGWN 322 Query: 300 PE 301 PE Sbjct: 323 PE 324 >B4AL30.1 B4AL30_BACPU Pectinesterase Length = 326 Score = 259 bits (661), Expect = 3e-67, Method: Compositional matrix adjust. Identities = 135/302 (44%), Positives = 187/302 (61%), Gaps = 12/302 (3%) Query: 5 EPQMLITVSKSVGEGFSSLQAALDSIPQEYKEAVTIRIKPGIYEEKVTVKQGTPPVLLLG 64 + ++ V + F ++Q+A+D+IP ++ VTI IK G+Y+EK+ + Q P V +G Sbjct: 30 QTNRVLVVDQKGNGSFRTVQSAIDAIPVNNQQRVTIYIKNGVYKEKILLPQNKPYVSFIG 89 Query: 65 EDTNSTIITWSDNAHTLGPDGEPLGTFKSGTLNVFAEHFTAENLTIRNASGPGTGQAVAA 124 ED TI+T+ D + T S + + A F AEN+T +N +G GQAVA Sbjct: 90 EDQYKTILTYHD------TNASTGSTTNSSSTMIRANDFYAENITFQNTAGRHAGQAVAL 143 Query: 125 FVDAGHAVFRRVRLLGDQDTLY-TGQGKQYYNECYIEGDVDYIFGAATALFERCRLHNKR 183 +V A F+++R+LG QDTLY TG G+QYY CYIEG VD+IFG+ATA+F+R + + Sbjct: 144 YVSGDRAAFKQIRVLGYQDTLYATGTGRQYYENCYIEGTVDFIFGSATAVFKRAEIKS-L 202 Query: 184 SRGYITAASTPEGAPFGYVFLDCEITSGEGVSE-VYLGRPWRPYAHVAFIRTVMDGSIIG 242 GYITAAST E +GYVF+D + G ++ VYLGRPWRP++ V F+ T MD I Sbjct: 203 GNGYITAASTTEAQKYGYVFIDSTLKKGTAAAQSVYLGRPWRPHSAVTFLYTKMDDHIKV 262 Query: 243 EGWHNWGQPDREETSRYEEYGSSGPGANPEARAAWSRQLIPQEAAQYRVLSVL---DGWH 299 +GWHNW D E T+RY+EYGS+G G+N R WS L EA+Q V +VL DGW+ Sbjct: 263 DGWHNWDNRDNERTARYKEYGSTGAGSNAANRVKWSSILSKNEASQITVQAVLSGTDGWN 322 Query: 300 PE 301 PE Sbjct: 323 PE 324 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.135 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,002,226,228 Number of extensions: 90916059 Number of successful extensions: 209099 Number of sequences better than 1.0e-05: 845 Number of HSP's gapped: 207137 Number of HSP's successfully gapped: 860 Length of query: 303 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 168 Effective length of database: 1,788,859,178 Effective search space: 300528341904 Effective search space used: 300528341904 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)