BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0352.0003.p (521 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q8EPL4.1 Q8EPL4_OCEIH Xylosidase : arabinofuranosidase EC=3.2.1.... 694 0.0 Q5WIW9.1 Q5WIW9_BACSK Xylosidase/arabinosidase EC=3.2.1.37 EC=3.... 626 e-177 B8I1U1.1 B8I1U1_CLOCE Glycoside hydrolase family 43 588 e-166 A9AZL2.1 A9AZL2_HERA2 Glycoside hydrolase family 43 567 e-159 P45982.1 XYLB_BUTFI Xylosidase/arabinosidase Beta-xylosidase Alp... 454 e-126 >Q8EPL4.1 Q8EPL4_OCEIH Xylosidase : arabinofuranosidase EC=3.2.1.37 EC=3.2.1.55 Length = 524 Score = 694 bits (1792), Expect = 0.0, Method: Compositional matrix adjust. Identities = 320/521 (61%), Positives = 401/521 (76%), Gaps = 3/521 (0%) Query: 1 MRTFRNPVLPGFYPDPSAIRVGEDYYLVTSSFEFFPGVPIFHSKDLVNWRQLGHVLDRPS 60 ++TF+NP++PGFYPDPS RV EDYYLVTS+FE+FPGVPIFHS+DLVNW Q+GHVLDRPS Sbjct: 3 LKTFKNPIIPGFYPDPSICRVDEDYYLVTSTFEYFPGVPIFHSRDLVNWNQIGHVLDRPS 62 Query: 61 QLNLDHTIPSMGIWAPTLRYHEGVFYMITTYVDNDKN-QHNFYVTATDPAGDWSDPVWLE 119 QLNL+ S GI+APT+RYH G+FYMITT+V++ + +FYVTA DP G WSDP WL Sbjct: 63 QLNLNGIPCSRGIYAPTIRYHNGIFYMITTFVESRTGLRRDFYVTAEDPGGPWSDPNWLG 122 Query: 120 DAPGIDSSLFFDDDGKVYYTGNRVPPEGQDYPKHMDIWLQEIDLSQGRLIGPKHSIWQGA 179 PGID SLFFDD+GKVYYT NR PP+GQ+YPKHM+I+LQE+DL +LIG K+S+W GA Sbjct: 123 PTPGIDPSLFFDDNGKVYYTANRQPPDGQEYPKHMEIYLQELDLESKQLIGDKYSLWDGA 182 Query: 180 LKVAHAQEGPHIYKIGAWYYVLIAEGGTGHTHAITIARSKSVTGPYEGHKANPILTHRHL 239 LK AHAQE PH+YK+ WYY++IAEGGTG THA+TIARS+S+ GPYE K NPILTHRHL Sbjct: 183 LKNAHAQEAPHLYKVNGWYYLMIAEGGTGFTHAVTIARSRSLLGPYESAKTNPILTHRHL 242 Query: 240 GRAYPIVNVGHGELVETQHGDWWMFCLASRTCGGYYRNLGRETFLTPVIWENEWPVVNPG 299 GR YPI N+GH +++ET G+WWM CL SR GG+YRNLGRETFL P++WEN+WPV+NPG Sbjct: 243 GREYPISNIGHADIIETHKGEWWMVCLGSRPYGGHYRNLGRETFLVPMVWENDWPVINPG 302 Query: 300 KGVLELETPAPDLPETRWPELPARDEFTGSELGLIWNFLRTPRGEFWSLTEKPGFLRLHL 359 KG++ELE+P PDL E +WPE+ RD+F +EL L WNF+RTP FWSL E+PG+LRL L Sbjct: 303 KGIVELESPFPDLDEVKWPEVSDRDDFNSTELSLQWNFVRTPHETFWSLNERPGYLRLKL 362 Query: 360 KAERLSEIANPSFIGRRQQHLSFRAAADMTFRPAAEGEVAGLVLLQNADYHFRYEYGLEA 419 K E + + NPSFIGRRQQH +F A M F P GEVAG+VLLQN D+ FR E L+ Sbjct: 363 KPETIIDRVNPSFIGRRQQHKNFVATTLMEFVPKKSGEVAGIVLLQNNDFQFRMELLLDG 422 Query: 420 GERVLQLIQRKGGSEQLLASVPYPQDQVQLKVEARGQEYSFYYRSSEAAKWTAFFERADG 479 +++LI+R G +Q+++ ++++ LKVEA+ Q+Y FYY +S DG Sbjct: 423 ASTIVRLIRRYDGIDQVISQEQTSENKLYLKVEAKEQDYCFYYGNS--LNNFELLANQDG 480 Query: 480 RVLSTDLAGGFTGAYIGMYTSSQGAESESYADFDWFSYEGL 520 ++LSTD+AGGF G YIGMY SS G ES + ADFDWF Y+ + Sbjct: 481 KLLSTDIAGGFVGTYIGMYASSNGKESTNKADFDWFEYKEI 521 >Q5WIW9.1 Q5WIW9_BACSK Xylosidase/arabinosidase EC=3.2.1.37 EC=3.2.1.55 Length = 523 Score = 626 bits (1614), Expect = e-177, Method: Compositional matrix adjust. Identities = 297/522 (56%), Positives = 376/522 (72%), Gaps = 5/522 (0%) Query: 1 MRTFRNPVLPGFYPDPSAIRVGEDYYLVTSSFEFFPGVPIFHSKDLVNWRQLGHVLDRPS 60 M F NP++PGFYPDPS RV EDYYLVTS+FE+FPGVPIFHS DLVNWRQ+GHVLDRP Sbjct: 1 MEPFNNPIIPGFYPDPSICRVNEDYYLVTSTFEYFPGVPIFHSTDLVNWRQIGHVLDRPE 60 Query: 61 QLNLDHTIPSMGIWAPTLRYHEGVFYMITTYVDNDKN-QHNFYVTATDPAGDWSDPVWLE 119 QLNLD T S GI+APT+RY+ G+FYMITT+V++ + NF+VTA DPAG WSDPVW++ Sbjct: 61 QLNLDGTPNSRGIYAPTIRYNNGLFYMITTFVESQHGARRNFFVTAKDPAGPWSDPVWIK 120 Query: 120 DAPGIDSSLFFDDDGKVYYTGNRVPPEGQDYPKHMDIWLQEIDLSQGRLIGPKHSIWQGA 179 APGID SLFFDDDG+VYYTGNRVPP GQ + KHM+IWLQEID G L+G IW GA Sbjct: 121 GAPGIDPSLFFDDDGRVYYTGNRVPPTGQTHAKHMEIWLQEIDPITGCLLGEPTGIWDGA 180 Query: 180 LKVAHAQEGPHIYKIGAWYYVLIAEGGTGHTHAITIARSKSVTGPYEGHKANPILTHRHL 239 LK+AHAQE PH+YK +YY+ IAEGGTG THA+T+ARS+ + GPYE ++ NP+LTHRHL Sbjct: 181 LKIAHAQEAPHLYKKDGYYYLFIAEGGTGFTHAVTVARSREIAGPYEANRRNPVLTHRHL 240 Query: 240 GRAYPIVNVGHGELVETQHGDWWMFCLASRTCGGYYRNLGRETFLTPVIWENEWPVVNPG 299 G+ YPI NVGH ++V+TQ +WW+ CL SR GG YRN+GRETFL P+ WE++WPVV P Sbjct: 241 GQDYPITNVGHADIVQTQDDEWWLVCLGSRPYGGMYRNMGRETFLAPMRWEDDWPVVCPE 300 Query: 300 KGVLELETPAPDLPETRWPELPARDEFTGSELGLIWNFLRTPRGEFWSLTEKPGFLRLHL 359 G +E+E P P LP + LP RD+F +LG WN +RTPR F+SL + G+L+L++ Sbjct: 301 TGKIEVEMPGPSLPRQKVEPLPVRDDFNARKLGFQWNMIRTPRTPFYSLEARKGYLQLNV 360 Query: 360 KAERLSEIANPSFIGRRQQHLSFRAAADMTFRPAAEGEVAGLVLLQNADYHFRYEYGLEA 419 + E L++ ANPSFIGRRQQH SF A M F P GE AGL L + H+R+E + + Sbjct: 361 RPETLADQANPSFIGRRQQHRSFTVRAFMEFDPKQSGEAAGLALFYSQKNHYRFEL-IRS 419 Query: 420 GERVLQLIQRKGGSEQLL--ASVPYPQDQVQLKVEARGQEYSFYYRSSEAAKWTAFFERA 477 G+ ++L++ + G E + ++ P P ++ +V A QE SF YR KW + Sbjct: 420 GQLFIRLVKVEDGVETVAGKSTAPIPATAIEWQVAADLQELSFSYRVP-GGKWRMVANKQ 478 Query: 478 DGRVLSTDLAGGFTGAYIGMYTSSQGAESESYADFDWFSYEG 519 D R+LSTD AGGF G YIGM+ +S G S + A FDWF Y+G Sbjct: 479 DSRLLSTDRAGGFVGTYIGMFATSNGQNSTTTAAFDWFEYKG 520 >B8I1U1.1 B8I1U1_CLOCE Glycoside hydrolase family 43 Length = 519 Score = 588 bits (1515), Expect = e-166, Method: Compositional matrix adjust. Identities = 283/517 (54%), Positives = 365/517 (70%), Gaps = 6/517 (1%) Query: 4 FRNPVLPGFYPDPSAIRVGEDYYLVTSSFEFFPGVPIFHSKDLVNWRQLGHVLDRPSQLN 63 F NP+LPGFYPDPS RV +DYYLVTSSF +FPG+PIFHSKDLVNWRQ+GH LDRPSQL+ Sbjct: 9 FSNPILPGFYPDPSICRVEDDYYLVTSSFTYFPGLPIFHSKDLVNWRQIGHALDRPSQLD 68 Query: 64 LDHTIPSMGIWAPTLRYHEGVFYMITTYVDNDKNQHNFYVTATDPAGDWSDPVWLEDAPG 123 LD S G++APT+RY+ G+FY+ T N + NF +T+ P G WSDP W+ DAPG Sbjct: 69 LDGLEQSQGLYAPTIRYNNGIFYIACT---NVGKKGNFIITSEKPEGPWSDPYWIADAPG 125 Query: 124 IDSSLFFDDDGKVYYTGNRVPPEGQDYPKHMDIWLQEIDLSQGRLIGPKHSIWQGALKVA 183 ID SLFFDDDGKVY+TG P+G Y + +IW++E+D + +L GP++ +W+GALK A Sbjct: 126 IDPSLFFDDDGKVYFTGTNDSPDGTYYGDN-EIWMRELDTGKMQLTGPRYGLWRGALKNA 184 Query: 184 HAQEGPHIYKIGAWYYVLIAEGGTGHTHAITIARSKSVTGPYEGHKANPILTHRHLGRAY 243 E PHIYKI +YY++IAEGGT + H++TIARS+ +TGPYEG+ NPI+THRHLGR Y Sbjct: 185 IWSEAPHIYKINGYYYLMIAEGGTDYHHSVTIARSREITGPYEGYIGNPIITHRHLGRKY 244 Query: 244 PIVNVGHGELVETQHGDWWMFCLASRTCGGYYRNLGRETFLTPVIWENEWPVVNPGKGVL 303 PI NVGH +LVETQ+G+WWM LASR GG+YRNLGRETFL PV WE+ WPVV+P G + Sbjct: 245 PIANVGHADLVETQNGEWWMVALASRPYGGHYRNLGRETFLIPVEWEDGWPVVSPLSGKV 304 Query: 304 ELETPAPDLPETRWPELPARDEFTGSELGLIWNFLRTPRGEFWSLTEKPGFLRLHLKAER 363 E P L E+ A D F +L IWNF+RTPR F+SLT++PG LRL+LK+ + Sbjct: 305 EFSYQRPALSPDNPVEVTACDHFDNEKLSFIWNFIRTPRENFYSLTDRPGHLRLNLKSPK 364 Query: 364 LSEIANPSFIGRRQQHLSFRAAADMTFRPAAEGEVAGLVLLQNADYHFRYEYGLEAGERV 423 + E NPSFIGRRQQH++FRA M F P E E AG++L+Q+ +YH R+E + V Sbjct: 365 IKEQKNPSFIGRRQQHINFRAKTVMEFVPGNENEAAGILLIQSNNYHMRFECTKSGEKDV 424 Query: 424 LQLIQRKGGSEQLLASVPYPQDQVQLKVEARGQEYSFYYRSSEAAKWTAFFERADGRVLS 483 ++LI G E ++A ++ + V+A GQ+YSFY + + DGR+LS Sbjct: 425 VRLIVCNDGKESIVAQRENTYTRIHMVVQAYGQDYSFY--CGDENELVELAVNVDGRILS 482 Query: 484 TDLAGGFTGAYIGMYTSSQGAESESYADFDWFSYEGL 520 TD+AGGF G Y+GM+TSS G +S + ADFD F Y GL Sbjct: 483 TDVAGGFVGTYVGMFTSSNGFDSSNMADFDLFEYTGL 519 >A9AZL2.1 A9AZL2_HERA2 Glycoside hydrolase family 43 Length = 516 Score = 567 bits (1461), Expect = e-159, Method: Compositional matrix adjust. Identities = 277/516 (53%), Positives = 360/516 (69%), Gaps = 7/516 (1%) Query: 2 RTFRNPVLPGFYPDPSAIRVGEDYYLVTSSFEFFPGVPIFHSKDLVNWRQLGHVLDRPSQ 61 R F NP++ GFYPDP+ RVGEDYYL+ S+FE+FPGVPI HS+DLV+W+Q+GH+LDRPSQ Sbjct: 3 RRFHNPIMTGFYPDPAICRVGEDYYLIHSTFEYFPGVPIHHSRDLVHWQQIGHILDRPSQ 62 Query: 62 LNLDHTIPSMGIWAPTLRYHEGVFYMITTYVDNDKNQHNFYVTATDPAGDWSDPVWLEDA 121 LNLD PS GI+APT+ YH+G FYMITT + + NF VTA PAG WSDP WL DA Sbjct: 63 LNLDEIHPSAGIFAPTISYHDGTFYMITTLIAGKERHGNFIVTAQSPAGPWSDPYWL-DA 121 Query: 122 PGIDSSLFFDDDGKVYYTGNRVPPEGQDYPKHMDIWLQEIDLSQGRLIGPKHSIWQGALK 181 GID SLFF+D G+ +Y GNR +Y +IWLQE+DL+ +LIG + +W+GAL Sbjct: 122 DGIDPSLFFED-GRAWYVGNR-GKANPEYEGQCEIWLQELDLTTMQLIGEQAVLWRGALN 179 Query: 182 VAHAQEGPHIYKIGAWYYVLIAEGGTGHTHAITIARSKSVTGPYEGHKANPILTHRHLGR 241 EGPH+YKI WYY+LIAE GT + HA+TIARS +T Y G+ ANPILTHR LGR Sbjct: 180 GVIWTEGPHLYKIDGWYYLLIAEAGTEYNHAVTIARSSELTQGYIGYPANPILTHRQLGR 239 Query: 242 AYPIVNVGHGELVETQHGDWWMFCLASRTCGGYYRNLGRETFLTPVIWENEWPVVNPGKG 301 YP++ GH +LV+TQ+G+WW+ LA R GG + NLGRETFL PV WE WP+++PG G Sbjct: 240 DYPVMGTGHSDLVQTQNGEWWLVLLAMRPYGGGFYNLGRETFLAPVQWEQGWPLISPGTG 299 Query: 302 VLELETPAPDLPETRWPELPARDEFTGSELGLIWNFLRTPRGEFWSLTEKPGFLRLHLKA 361 +EL PAPDLP RWP A D F G L + W FLRTPR ++WSL+E+ G+LR+ L+ Sbjct: 300 KVELSYPAPDLPLQRWPVQAACDHFDGDNLAMHWMFLRTPRSQWWSLSERVGWLRMQLRP 359 Query: 362 ERLSEIANPSFIGRRQQHLSFRAAADMTFRPAAEGEVAGLVLLQNADYHFRYEYGLEAGE 421 E+++++ NPSF+GRRQQH++F A + F+P EVAG+VL+QN +Y ++ + GE Sbjct: 360 EQINQMVNPSFVGRRQQHMNFLAQTMLEFQPQQPQEVAGMVLIQNHNYQVQF---VITGE 416 Query: 422 RVLQLIQRKGGSEQLLASVPYPQDQVQLKVEARGQEYSFYYRSSEAAKWTAFFERADGRV 481 + LI + G ++ LA VP + L++ A GQEYSF+ + + W FE DGR Sbjct: 417 QQASLIVCRNGEQECLAQVPIASQRNYLRIVAYGQEYSFFV-AEQPDAWRPVFENLDGRF 475 Query: 482 LSTDLAGGFTGAYIGMYTSSQGAESESYADFDWFSY 517 LST +AGGF G IG+Y SSQG S++ ADFDWF Y Sbjct: 476 LSTPVAGGFVGTVIGLYASSQGQTSQTVADFDWFEY 511 >P45982.1 XYLB_BUTFI Xylosidase/arabinosidase Beta-xylosidase Alpha-N-arabinofuranosidase EC=3.2.1.37 EC=3.2.1.55 Length = 517 Score = 454 bits (1168), Expect = e-126, Method: Compositional matrix adjust. Identities = 245/523 (46%), Positives = 329/523 (62%), Gaps = 13/523 (2%) Query: 1 MRTFRNPVLPGFYPDPSAIRVGEDYYLVTSSFEFFPGVPIFHSKDLVNWRQLGHVLDRPS 60 M NP+L GFYPDPS R G+D+YLV SSF + PGVPIFH+KDL ++ Q+G++LDR S Sbjct: 1 MVIANNPILKGFYPDPSICRKGDDFYLVCSSFVYAPGVPIFHTKDLAHFEQIGNILDRES 60 Query: 61 QLNLDHTIPSMGIWAPTLRYHEGVFYMITTYVDNDKNQHNFYVTATDPAGDWSDPVWL-- 118 QL L I S GI+APT+R H G+FYMITT N + NF VTA DPAG WS+P +L Sbjct: 61 QLPLSGDI-SRGIFAPTIREHNGIFYMITT---NVSSGGNFIVTAKDPAGPWSEPYYLGE 116 Query: 119 EDAPGIDSSLFFDDDGKVYYTGNRVPPEGQDYPKHMDIWLQEIDLSQGRLIGPKHSIWQG 178 ++APGID SLFFDDDGK YY G R P+G Y +IW+QE+DL Q +L+GP +IW+G Sbjct: 117 DEAPGIDPSLFFDDDGKCYYVGTRPNPDGVRYNGDWEIWVQELDLEQMKLVGPSMAIWKG 176 Query: 179 ALKVAHAQEGPHIYKIGAWYYVLIAEGGTGHTHAITIARSKSVTGPYEGHKANPILTHRH 238 ALK EGPH+YK +YY+L AE GT HAI++ARSK + +EG NPI THR+ Sbjct: 177 ALKDVIWPEGPHLYKKDGYYYLLHAEAGTSFEHAISVARSKELFKWFEGCPRNPIFTHRN 236 Query: 239 LGRAYPIVNVGHGELVETQHGDWWMFCLASRTCGGYYRNLGRETFLTPVIWENEWPVVNP 298 LG+ YP+ NVGH +LV+ +G+W+M LASR C G +LGRETFL VIWE+ WPVVNP Sbjct: 237 LGKDYPVCNVGHADLVDDINGNWYMVMLASRPCKGKC-SLGRETFLAKVIWEDGWPVVNP 295 Query: 299 GKGVLELETPAPDLPETRW-PELPARDEFTGSELGLIWNFLRTPR--GEFWSLTEKPGFL 355 G G L E DLPE R+ E+ +D+ T E L F+ R +F+SLT+ PGFL Sbjct: 296 GVGRLTDEVEM-DLPEYRFSKEITTKDKMTFEETVLDDRFVGIERRSEDFYSLTDNPGFL 354 Query: 356 RLHLKAERLSEIANPSFIGRRQQHLSFRAAADMTFRPAAEGEVAGLVLLQNADYHFRYEY 415 RL L+ E + NPS++G RQ+ SFRA+ + F PA + E AG+VL QN + H Sbjct: 355 RLKLRPEAIENTGNPSYLGIRQKTHSFRASCGLKFTPAKDNECAGMVLFQNNENHLELLV 414 Query: 416 GLEAGERVLQLIQRKGGSEQLLASVPYPQDQVQLKVEARGQEYSFYYRSSEAAKWTAFFE 475 + + ++ G++ LA+ +++ +EA Q + Y + + A E Sbjct: 415 VKKKDKLQFKVGPVIKGTKIRLATFDISSGDLEIILEAANQLANIYIKKNNEKILVA--E 472 Query: 476 RADGRVLSTDLAGGFTGAYIGMYTSSQGAESESYADFDWFSYE 518 D +T+ +GGF G IG+Y SS G S++Y D+ +F+ E Sbjct: 473 CIDLSPYTTEESGGFVGCTIGLYASSNGKTSDNYCDYSYFTVE 515 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.138 0.445 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,681,069,285 Number of extensions: 172463272 Number of successful extensions: 301901 Number of sequences better than 1.0e-05: 634 Number of HSP's gapped: 298845 Number of HSP's successfully gapped: 662 Length of query: 521 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 381 Effective length of database: 1,741,754,103 Effective search space: 663608313243 Effective search space used: 663608313243 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)