BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0351.0013.p (300 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KFP9.1 B3KFP9_9BACI 4-deoxy-L-threo-5-hexosulose-uronate ketol... 464 e-129 B1DJQ5.1 B1DJQ5_9BACL 4-deoxy-L-threo-5-hexosulose-uronate ketol... 378 e-103 C3WD24.1 C3WD24_FUSMR 5-keto-4-deoxyuronate isomerase 348 3e-94 A9KNI2.1 KDUI_CLOPH 4-deoxy-L-threo-5-hexosulose-uronate ketol-i... 348 4e-94 B1BAQ7.1 B1BAQ7_CLOBO 4-deoxy-L-threo-5-hexosulose-uronate ketol... 347 7e-94 >B3KFP9.1 B3KFP9_9BACI 4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase EC=5.3.1.17 Length = 277 Score = 464 bits (1195), Expect = e-129, Method: Compositional matrix adjust. Identities = 210/277 (75%), Positives = 245/277 (88%) Query: 24 MERRFASHPNEVKQFDTARLRKEFHIPVIFAPDELKLVLTHEDRMIVGGANPVNKDVALT 83 MERR+ASHPNEVKQFDT RLRKEFHIPV+F PD+L+LVLTHEDR+I+GGA PV + V L Sbjct: 1 MERRYASHPNEVKQFDTERLRKEFHIPVLFTPDKLELVLTHEDRLIIGGAAPVREQVKLD 60 Query: 84 TDLKELGVTYFLERRELGVINVGGKGSVVVDGTEYEIDFKECLYVGQGAKDVIFKSADSA 143 TDLKELGV+ FLERRELG+IN+GG G+V+VDGTE+E++ KECLYVG G ++VIF+S D A Sbjct: 61 TDLKELGVSSFLERRELGIINIGGHGTVLVDGTEHELNNKECLYVGMGTEEVIFQSTDPA 120 Query: 144 KPAKFYLNSAPAHQSYPTTKTTLAESESGAMGGLENSNERTIHRFIHAGGVQSAQLVMGM 203 PAKFYLNSAPAHQS+PT K TL E++S A+G ++NSN+R I+RFIH G+QSAQLVMGM Sbjct: 121 NPAKFYLNSAPAHQSFPTAKATLEEADSSALGDIKNSNDRVIYRFIHQKGIQSAQLVMGM 180 Query: 204 TQLKPGSMWNTMPSHTHPRRMEAYFYFDLPDDSIVFHLMGEPTETRHIVMHNEQAVISPS 263 T LK G+MWNTMP+HTHPRRMEAY YFDLP+D++ HLMGEP+ETRHIVM NEQAVISPS Sbjct: 181 TLLKEGNMWNTMPAHTHPRRMEAYLYFDLPEDAVAIHLMGEPSETRHIVMRNEQAVISPS 240 Query: 264 WSIHSGVGTHNYTFIWSMAGDNKRYDDMDPVGMKELQ 300 WSIHSGVGT NYTFIW MAGDNKRYDDMDPV MK+L+ Sbjct: 241 WSIHSGVGTSNYTFIWGMAGDNKRYDDMDPVSMKDLK 277 >B1DJQ5.1 B1DJQ5_9BACL 4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase EC=5.3.1.17 Length = 277 Score = 378 bits (971), Expect = e-103, Method: Compositional matrix adjust. Identities = 168/277 (60%), Positives = 213/277 (76%) Query: 24 MERRFASHPNEVKQFDTARLRKEFHIPVIFAPDELKLVLTHEDRMIVGGANPVNKDVALT 83 ME R+ASHP EVK FDT RLR+EF + +F D+L L +H DR I+GGA PVN+ V L Sbjct: 1 MEIRYASHPQEVKAFDTTRLRQEFLLETLFVQDQLTLTYSHVDRFIIGGAVPVNESVKLE 60 Query: 84 TDLKELGVTYFLERRELGVINVGGKGSVVVDGTEYEIDFKECLYVGQGAKDVIFKSADSA 143 D K +G FLERRE+G+INVGG+G+V VDGT Y+++ K+CLY+G GA +V+F S+DSA Sbjct: 61 ADTKTMGAATFLERREIGIINVGGRGTVTVDGTAYDMENKDCLYIGLGAAEVVFTSSDSA 120 Query: 144 KPAKFYLNSAPAHQSYPTTKTTLAESESGAMGGLENSNERTIHRFIHAGGVQSAQLVMGM 203 PAKFYLNS PAH++YPT K +++E+ +G L +NERTI+R+IH GG+QS QLVMG+ Sbjct: 121 NPAKFYLNSTPAHKNYPTVKASISEASPNHLGALNTNNERTIYRYIHTGGIQSCQLVMGL 180 Query: 204 TQLKPGSMWNTMPSHTHPRRMEAYFYFDLPDDSIVFHLMGEPTETRHIVMHNEQAVISPS 263 T LKPGS WNTMP HTH RR E Y YFD+ D VFH MGEP ETRH+V+ NEQA++SPS Sbjct: 181 TMLKPGSNWNTMPCHTHNRRSEVYLYFDMQADGNVFHFMGEPQETRHLVVRNEQAILSPS 240 Query: 264 WSIHSGVGTHNYTFIWSMAGDNKRYDDMDPVGMKELQ 300 WSIHSGVGT YTFIW MAG+N+ ++DMD V ++L+ Sbjct: 241 WSIHSGVGTSAYTFIWGMAGENQIFEDMDAVAPQDLK 277 >C3WD24.1 C3WD24_FUSMR 5-keto-4-deoxyuronate isomerase Length = 278 Score = 348 bits (893), Expect = 3e-94, Method: Compositional matrix adjust. Identities = 161/277 (58%), Positives = 203/277 (73%), Gaps = 1/277 (0%) Query: 24 MERRFASHPNEVKQFDTARLRKEFHIPVIFAPDELKLVLTHEDRMIVGGANPVNKDVALT 83 ++ R+A+HP + K + T LRK + I +F DE+ LV +H DR+I GG PV + + L Sbjct: 3 LDVRYANHPEDSKHYTTEELRKHYLIEKVFIEDEVNLVYSHVDRVIAGGITPVKESLKLE 62 Query: 84 TDLKELGVTYFLERRELGVINVGGKGSVVVDGTEYEIDFKECLYVGQGAKDVIFKSADSA 143 KELG YFLERRELGVIN+GG G + +DG YE+ +E LYVG G+K++IF S D Sbjct: 63 -GCKELGSEYFLERRELGVINIGGNGKITIDGKVYEVKSREGLYVGMGSKELIFTSDDEK 121 Query: 144 KPAKFYLNSAPAHQSYPTTKTTLAESESGAMGGLENSNERTIHRFIHAGGVQSAQLVMGM 203 PAKFY+NS+PAH++YPT L ++ +G LENSN+RTI++++H QS QLVMGM Sbjct: 122 NPAKFYINSSPAHKTYPTVMIDLEKANKVHLGDLENSNKRTIYQYVHPAVCQSCQLVMGM 181 Query: 204 TQLKPGSMWNTMPSHTHPRRMEAYFYFDLPDDSIVFHLMGEPTETRHIVMHNEQAVISPS 263 T L+P +MWNTMP HTH RRME Y YFD+ +DS VFHLMGEP ETRHIVM NEQAVISPS Sbjct: 182 TMLEPNNMWNTMPCHTHERRMEVYLYFDMTEDSKVFHLMGEPNETRHIVMGNEQAVISPS 241 Query: 264 WSIHSGVGTHNYTFIWSMAGDNKRYDDMDPVGMKELQ 300 WSIHSGVGT NYTFIW MAG+N+ + DMD V M +L+ Sbjct: 242 WSIHSGVGTRNYTFIWGMAGENQTFTDMDHVAMNDLR 278 >A9KNI2.1 KDUI_CLOPH 4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase EC=5.3.1.17 Length = 276 Score = 348 bits (893), Expect = 4e-94, Method: Compositional matrix adjust. Identities = 160/277 (57%), Positives = 205/277 (74%), Gaps = 1/277 (0%) Query: 24 MERRFASHPNEVKQFDTARLRKEFHIPVIFAPDELKLVLTHEDRMIVGGANPVNKDVALT 83 ME R A+ P +VK + T RLR+EF I +F +E+KLV +H DR+I G A PVN+++ LT Sbjct: 1 MELRTAASPKDVKHYTTDRLREEFLIDDLFKVNEIKLVYSHIDRIITGSAVPVNQELKLT 60 Query: 84 TDLKELGVTYFLERRELGVINVGGKGSVVVDGTEYEIDFKECLYVGQGAKDVIFKSADSA 143 EL YFL+RRE+G+IN+GGKG++ +DGT YE+++K+ +Y+G GAKD+ F S D+ Sbjct: 61 AG-DELRAEYFLQRREMGIINIGGKGTITIDGTVYELEYKDGMYIGMGAKDISFASVDAT 119 Query: 144 KPAKFYLNSAPAHQSYPTTKTTLAESESGAMGGLENSNERTIHRFIHAGGVQSAQLVMGM 203 PAKFY+NSAPAH SYPT + +G LE SN R I ++I G V+S QLVMGM Sbjct: 120 NPAKFYINSAPAHTSYPTVLIKPENCVNVELGSLEGSNHRVICKYILPGQVESCQLVMGM 179 Query: 204 TQLKPGSMWNTMPSHTHPRRMEAYFYFDLPDDSIVFHLMGEPTETRHIVMHNEQAVISPS 263 TQLKPGS+WNTMP HTH RRME Y YFD+ ++ +VFH MGEPTETRHI+M NEQAVISPS Sbjct: 180 TQLKPGSVWNTMPCHTHDRRMEVYLYFDMQENDMVFHYMGEPTETRHIIMRNEQAVISPS 239 Query: 264 WSIHSGVGTHNYTFIWSMAGDNKRYDDMDPVGMKELQ 300 WSIHSG GT YTFIW M G+N+ +DDMD +K+L+ Sbjct: 240 WSIHSGCGTKAYTFIWGMVGENQDFDDMDACDLKDLR 276 >B1BAQ7.1 B1BAQ7_CLOBO 4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase EC=5.3.1.17 Length = 276 Score = 347 bits (890), Expect = 7e-94, Method: Compositional matrix adjust. Identities = 153/277 (55%), Positives = 210/277 (75%), Gaps = 1/277 (0%) Query: 24 MERRFASHPNEVKQFDTARLRKEFHIPVIFAPDELKLVLTHEDRMIVGGANPVNKDVALT 83 M+ R+A+HP + K++ T LRK +H+ +F D++ L +H DR++ GGA PV++++ L Sbjct: 1 MDIRYANHPEDSKKYTTEELRKHYHVQSVFTKDQVSLTYSHVDRIVFGGAMPVDQELQLV 60 Query: 84 TDLKELGVTYFLERRELGVINVGGKGSVVVDGTEYEIDFKECLYVGQGAKDVIFKSADSA 143 KE+GV+YFLERRE+G+INVGG G + +DGTEYE+ ++ LY+G G K+V FKS + Sbjct: 61 AG-KEMGVSYFLERREMGIINVGGDGVITLDGTEYEMKKRDGLYIGMGIKEVKFKSNNKD 119 Query: 144 KPAKFYLNSAPAHQSYPTTKTTLAESESGAMGGLENSNERTIHRFIHAGGVQSAQLVMGM 203 PAKFY+NS PAH+SYP K +A++ G EN N+RTI++++H +S QL+MGM Sbjct: 120 NPAKFYINSVPAHKSYPIVKIDIAKANPVCCGEQENVNKRTIYQYVHPAVCESCQLLMGM 179 Query: 204 TQLKPGSMWNTMPSHTHPRRMEAYFYFDLPDDSIVFHLMGEPTETRHIVMHNEQAVISPS 263 T L+PG++WNTMP HTH RRME YFYFD+ +D+ VFHLMGEP+ETRHIVM NE+ VISPS Sbjct: 180 TVLEPGNVWNTMPCHTHERRMEVYFYFDMNEDTRVFHLMGEPSETRHIVMKNEECVISPS 239 Query: 264 WSIHSGVGTHNYTFIWSMAGDNKRYDDMDPVGMKELQ 300 WSIHSGVGT NYTFIW M G+N+ +DDMD + M++L+ Sbjct: 240 WSIHSGVGTSNYTFIWGMCGENQTFDDMDHIKMQDLK 276 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.134 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,934,540,107 Number of extensions: 82317096 Number of successful extensions: 163930 Number of sequences better than 1.0e-05: 289 Number of HSP's gapped: 163578 Number of HSP's successfully gapped: 289 Length of query: 300 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 165 Effective length of database: 1,788,859,178 Effective search space: 295161764370 Effective search space used: 295161764370 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)