BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0351.0011.p (216 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KFP7.1 B3KFP7_9BACI 2-deoxy-D-gluconate 3-dehydrogenase 335 2e-90 B1DJQ7.1 B1DJQ7_9BACL 2-deoxy-D-gluconate 3-dehydrogenase 313 5e-84 C0VC04.1 C0VC04_9MICO 2-deoxy-D-gluconate 3-dehydrogenase EC=1.1... 260 6e-68 Q8PE31.1 Q8PE31_XANCP 2-deoxy-D-gluconate 3-dehydrogenase 258 2e-67 Q4V0C5.1 Q4V0C5_XANC8 2-deoxy-D-gluconate 3-dehydrogenase 258 2e-67 >B3KFP7.1 B3KFP7_9BACI 2-deoxy-D-gluconate 3-dehydrogenase Length = 250 Score = 335 bits (858), Expect = 2e-90, Method: Compositional matrix adjust. Identities = 157/216 (72%), Positives = 180/216 (83%) Query: 1 VISASLNASDETVAAAKAFGVKALSIAGDLSDHTKLQGMFDEAVAFTGKVDILVNCAGMI 60 +++ S+N S ET+ A K FG KA I DLSD +KLQ FD+A+ TG VDILVN AG+I Sbjct: 35 IVAVSMNTSAETIEAIKGFGRKASEIRVDLSDQSKLQQTFDQALELTGHVDILVNNAGII 94 Query: 61 RRTPAKDHSEKDWFDVINLNLNTVFLLSQIAGRHFLERGNGKIINICSMLSYQGGINVPG 120 RRTPAKDH +DW DVI+LNLNTVFLLSQ+AGRH +ERG+GKIINICSMLSYQGGINVPG Sbjct: 95 RRTPAKDHGVQDWLDVIDLNLNTVFLLSQLAGRHMIERGSGKIINICSMLSYQGGINVPG 154 Query: 121 YTASKHGVAGLTKAFANEWAGSGLNINAIAPGYMATENTAPIRADQNRADSILDRIPAGR 180 YTASKHGVAGLTKA ANEWA SG+ +N IAPGY+ T+NTA IR D++R SI DRIPAGR Sbjct: 155 YTASKHGVAGLTKALANEWAASGIQVNGIAPGYIETDNTAAIRQDEDRRTSITDRIPAGR 214 Query: 181 WGTAEDVKGPAVFLASAASDYLNGHILNVDGGWMAR 216 WG ED+ GPAVFLASAASDY+NGH+L VDGGWMAR Sbjct: 215 WGKPEDIGGPAVFLASAASDYMNGHVLCVDGGWMAR 250 >B1DJQ7.1 B1DJQ7_9BACL 2-deoxy-D-gluconate 3-dehydrogenase Length = 249 Score = 313 bits (803), Expect = 5e-84, Method: Compositional matrix adjust. Identities = 147/216 (68%), Positives = 180/216 (83%) Query: 1 VISASLNASDETVAAAKAFGVKALSIAGDLSDHTKLQGMFDEAVAFTGKVDILVNCAGMI 60 V++ S + S+ TVAA + G +A I+ DLS+ L+ +F++A+ GKVDILVN AG+I Sbjct: 34 VVAISSSNSNATVAAIRELGGQAEEISADLSNADILEDVFNQALGLFGKVDILVNNAGII 93 Query: 61 RRTPAKDHSEKDWFDVINLNLNTVFLLSQIAGRHFLERGNGKIINICSMLSYQGGINVPG 120 RRTPA DH+ KDW+DVI+LNLN+VF LSQ+AGRH LERG+GK+IN+ SMLSYQGGINVPG Sbjct: 94 RRTPAADHAAKDWYDVIDLNLNSVFFLSQLAGRHMLERGSGKMINVASMLSYQGGINVPG 153 Query: 121 YTASKHGVAGLTKAFANEWAGSGLNINAIAPGYMATENTAPIRADQNRADSILDRIPAGR 180 YTASKH VAG+TKA ANEWAG G+N+NAIAPGYM T NTAPI D+ R +SI +RIPAGR Sbjct: 154 YTASKHAVAGVTKALANEWAGRGINVNAIAPGYMTTNNTAPILKDEARTNSITERIPAGR 213 Query: 181 WGTAEDVKGPAVFLASAASDYLNGHILNVDGGWMAR 216 WGT +D+KGP VFLAS+ASDYL+GH+LNVDGGWMAR Sbjct: 214 WGTPDDLKGPVVFLASSASDYLSGHVLNVDGGWMAR 249 >C0VC04.1 C0VC04_9MICO 2-deoxy-D-gluconate 3-dehydrogenase EC=1.1.1.125 Length = 254 Score = 260 bits (665), Expect = 6e-68, Method: Compositional matrix adjust. Identities = 129/218 (59%), Positives = 158/218 (72%), Gaps = 2/218 (0%) Query: 1 VISASLNASDETVAAAKAFGVKALSIAGDLS--DHTKLQGMFDEAVAFTGKVDILVNCAG 58 V+ + +T A +A G +A+ + DL+ D + + D+AV G+VDILVN AG Sbjct: 37 VVCVQRTGTPDTADAVRALGRRAVVLTADLAAADEESVAALLDDAVDEFGRVDILVNNAG 96 Query: 59 MIRRTPAKDHSEKDWFDVINLNLNTVFLLSQIAGRHFLERGNGKIINICSMLSYQGGINV 118 IRR+PA +H W DVI NL+ V+LLSQ GRH LE+G+G IINI SMLS+QGGI V Sbjct: 97 TIRRSPAAEHPRAWWDDVIATNLDAVWLLSQTFGRHMLEQGSGTIINIASMLSFQGGITV 156 Query: 119 PGYTASKHGVAGLTKAFANEWAGSGLNINAIAPGYMATENTAPIRADQNRADSILDRIPA 178 PGY ASKH VAGLTKA ANEWA G+ +NAIAPGYMAT+NTAP+RAD NRADSI DRIPA Sbjct: 157 PGYAASKHAVAGLTKALANEWAAHGVKVNAIAPGYMATDNTAPLRADTNRADSITDRIPA 216 Query: 179 GRWGTAEDVKGPAVFLASAASDYLNGHILNVDGGWMAR 216 GRWG A+D+ G VFLAS AS Y++G +L VDGGW+ R Sbjct: 217 GRWGNADDLAGAVVFLASDASAYVHGTVLPVDGGWLVR 254 >Q8PE31.1 Q8PE31_XANCP 2-deoxy-D-gluconate 3-dehydrogenase Length = 251 Score = 258 bits (660), Expect = 2e-67, Method: Compositional matrix adjust. Identities = 125/210 (59%), Positives = 156/210 (74%) Query: 7 NASDETVAAAKAFGVKALSIAGDLSDHTKLQGMFDEAVAFTGKVDILVNCAGMIRRTPAK 66 A ET KA G + ++I +L +Q + DE +A G++DILVN AG+IRRT A Sbjct: 42 QAPTETEEKVKAMGRRFVAIEANLISIEPVQRILDETLAGLGRLDILVNNAGLIRRTDAV 101 Query: 67 DHSEKDWFDVINLNLNTVFLLSQIAGRHFLERGNGKIINICSMLSYQGGINVPGYTASKH 126 D SE+DW DV+N+NL + F +SQ AGRHF+ +G+GKIINI SMLS+QGGI VP YTASK Sbjct: 102 DFSEQDWDDVMNVNLKSAFFMSQAAGRHFITQGSGKIINIASMLSFQGGIRVPSYTASKS 161 Query: 127 GVAGLTKAFANEWAGSGLNINAIAPGYMATENTAPIRADQNRADSILDRIPAGRWGTAED 186 G+AG+T+ ANEW G+ NAIAPGYMAT+NTA +RADQ+R SILDRIPA RWG D Sbjct: 162 GIAGITRLLANEWGSKGVTANAIAPGYMATDNTAQLRADQDRNKSILDRIPAARWGVPAD 221 Query: 187 VKGPAVFLASAASDYLNGHILNVDGGWMAR 216 + G AVFLAS+ASDY+NG I+ VDGGW+AR Sbjct: 222 LGGAAVFLASSASDYVNGAIIPVDGGWLAR 251 >Q4V0C5.1 Q4V0C5_XANC8 2-deoxy-D-gluconate 3-dehydrogenase Length = 251 Score = 258 bits (660), Expect = 2e-67, Method: Compositional matrix adjust. Identities = 125/210 (59%), Positives = 156/210 (74%) Query: 7 NASDETVAAAKAFGVKALSIAGDLSDHTKLQGMFDEAVAFTGKVDILVNCAGMIRRTPAK 66 A ET KA G + ++I +L +Q + DE +A G++DILVN AG+IRRT A Sbjct: 42 QAPTETEEKVKAMGRRFVAIEANLISIEPVQRILDETLAGLGRLDILVNNAGLIRRTDAV 101 Query: 67 DHSEKDWFDVINLNLNTVFLLSQIAGRHFLERGNGKIINICSMLSYQGGINVPGYTASKH 126 D SE+DW DV+N+NL + F +SQ AGRHF+ +G+GKIINI SMLS+QGGI VP YTASK Sbjct: 102 DFSEQDWDDVMNVNLKSAFFMSQAAGRHFITQGSGKIINIASMLSFQGGIRVPSYTASKS 161 Query: 127 GVAGLTKAFANEWAGSGLNINAIAPGYMATENTAPIRADQNRADSILDRIPAGRWGTAED 186 G+AG+T+ ANEW G+ NAIAPGYMAT+NTA +RADQ+R SILDRIPA RWG D Sbjct: 162 GIAGITRLLANEWGSKGVTANAIAPGYMATDNTAQLRADQDRNKSILDRIPAARWGVPAD 221 Query: 187 VKGPAVFLASAASDYLNGHILNVDGGWMAR 216 + G AVFLAS+ASDY+NG I+ VDGGW+AR Sbjct: 222 LGGAAVFLASSASDYVNGAIIPVDGGWLAR 251 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.133 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,286,974,161 Number of extensions: 52592803 Number of successful extensions: 199431 Number of sequences better than 1.0e-05: 23249 Number of HSP's gapped: 174751 Number of HSP's successfully gapped: 23317 Length of query: 216 Length of database: 3,060,696,203 Length adjustment: 130 Effective length of query: 86 Effective length of database: 1,835,964,253 Effective search space: 157892925758 Effective search space used: 157892925758 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)