BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0350.0045.p (471 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B7GJK8.1 B7GJK8_ANOFW Signal transduction histidine kinase 429 e-118 B1DIN8.1 B1DIN8_9BACL Histidine kinase internal region 426 e-117 B4BMS8.1 B4BMS8_9BACI Integral membrane sensor signal transducti... 290 1e-76 Q9K691.1 Q9K691_BACHD Two-component sensor histidine kinase 284 1e-74 Q5KUZ2.1 Q5KUZ2_GEOKA Two-component sensor histidine kinase 283 2e-74 >B7GJK8.1 B7GJK8_ANOFW Signal transduction histidine kinase Length = 479 Score = 429 bits (1102), Expect = e-118, Method: Compositional matrix adjust. Identities = 216/468 (46%), Positives = 313/468 (66%), Gaps = 3/468 (0%) Query: 1 MTIRAKLLIGIPLLVVLANTIAFFVFQSGKVVQRSYDEMLGRILLIEQSTEWADTNLKLL 60 M IR KL + IPLLV+L N IAFF+FQSGK VQ SYD M+ RI L +Q + N++ L Sbjct: 1 MKIRTKLFVFIPLLVLLLNCIAFFIFQSGKKVQESYDVMMQRIFLYKQISLETQENVRFL 60 Query: 61 YAFLLD-PRIDQAEMKSAGGQLERLSGEIKQEGGQTDVSFTMEDYISMTASFLEQKQAAI 119 ++L+ + ++ QLE+L E+ + + +FT+E+Y +M FL+ +QA I Sbjct: 61 SSYLIHQDEYNYKQLIEHKQQLEKLHRELTERQLDGNDAFTLENYKNMVYVFLDLEQAII 120 Query: 120 QASASEDPQIAFEHYVEAEKIVGYIREEGQQLVDAELAFYRPIIQNIREENDRMNRLGAA 179 + + Y E EKI +IRE+ Q LVD EL+ Y+P+ + I + RMN LG Sbjct: 121 HKLTKQQFTGYADQYNEIEKIASFIREDSQALVDVELSLYQPVYKQILQHTARMNELGGT 180 Query: 180 LFGMNALMGVLLAVWASRSITGPVGRLVGLAKRIATGNLDIG-PQPSRDDELGILSNAIQ 238 LF + ++ ++ A+W SR+I P+ LV +AK+I++G LD P+ DE+G+L Q Sbjct: 181 LFVLTTILSIVFAIWLSRTIVIPIHHLVHVAKKISSGQLDTRIPRFDGHDEIGLLGQTFQ 240 Query: 239 QMSADLSVLIERDKKSLEMQRLVKELELQALQSQINPHFLFNTLNVLSRLALLEGADRTS 298 M +L +LI ++ + LE ++LV+ELEL+ALQSQINPHFLFNTLNV+S+LA +EGA++TS Sbjct: 241 HMIENLRILISKNMEILEKEKLVRELELKALQSQINPHFLFNTLNVISKLAYIEGAEQTS 300 Query: 299 DLIVSMSNLLRYSLQKLDKPVTLQDEIAHIQEYAAIQQARFRERIRFEMDFDPSVLQEEI 358 +L VS SNLLRY+L+KLD+PVTL++E+ H +EY AIQ+ARFR+R+ FE+ D S L++ I Sbjct: 301 ELTVSTSNLLRYNLRKLDQPVTLREEVEHAKEYFAIQKARFRDRVTFEVKVDESCLEQPI 360 Query: 359 PALTLQPIVENAFLHGVANMESGAVISLVLRRTGGEVWIEISDNGVGMPEETLQSILRLE 418 P LTLQP++ENAF+HG+ ME GA I L++ + I I+DNGVGMP + ++I+ Sbjct: 361 PCLTLQPLIENAFIHGIEGMEEGANIQLIIEEKRNCIQITIADNGVGMPYDVQRAIMDAS 420 Query: 419 AG-AESGKSTGLGTRNVLKRLQLFFGEEDLVRIHSRLGQGTTITIRIP 465 ++G STGLG NVL+RLQLF+G E ++ I S +GT I +R+P Sbjct: 421 YSLTKTGHSTGLGLENVLRRLQLFYGVEKILEIKSAPNEGTAIILRLP 468 >B1DIN8.1 B1DIN8_9BACL Histidine kinase internal region Length = 491 Score = 426 bits (1095), Expect = e-117, Method: Compositional matrix adjust. Identities = 233/471 (49%), Positives = 323/471 (68%), Gaps = 9/471 (1%) Query: 1 MTIRAKLLIGIPLLVVLANTIAFFVFQSGKVVQRSYDEMLGRILLIEQSTEWADTNLKLL 60 MT+R KLL+ IPLLV+L N + FF+F+S K VQ SY+ M+ R+LL +S + AD N++ L Sbjct: 1 MTLRKKLLLFIPLLVLLTNGVTFFLFESSKTVQHSYNTMMDRMLLYTRSAQTADENVRAL 60 Query: 61 YAFLLDPRID-QAEMKSAGGQLERLSGEIKQEGGQTDVSFTMEDYISMTASFLEQKQAAI 119 Y +LL+P + +A ++ +L R+S ++ + + ++ ++ Y M + EQ QAA Sbjct: 61 YTYLLNPEDNGRASLRQQQAELARISEKLASQRTELGIAGVLQGYAHMLQTLAEQVQAAE 120 Query: 120 QASASEDPQIAFEHYVEAEKIVGYIREEGQQLVDAELAFYRPIIQNIREENDRMNRLGAA 179 +++ D A +Y + E+ +IREE ++L+D EL+ Y P+ ++I+ E RM+ LG A Sbjct: 121 KSTVPND---ALSYYEDTERTSSFIREEERRLIDLELSAYEPVYRSIQLEIVRMDWLGPA 177 Query: 180 LFGMNALMGVLLAVWASRSITGPVGRLVGLAKRIATGNLDIGPQPSR-DDELGILSNAIQ 238 +F +N L+ +++A+W SRSIT PV RLV A+ ++ G LD P R +DELGILS A + Sbjct: 178 VFIINTLLSIVVALWISRSITEPVDRLVQTAQHLSKGQLDTAYLPERSNDELGILSEAFR 237 Query: 239 QMSADLSVLIERDKKSLEMQRLVKELELQALQSQINPHFLFNTLNVLSRLALLEGADRTS 298 M DL+ L+ RDK+ +E +RLVKELELQALQSQINPHFLFNTLNVLS+LALLEGADRTS Sbjct: 238 HMIKDLNDLMGRDKERMESERLVKELELQALQSQINPHFLFNTLNVLSKLALLEGADRTS 297 Query: 299 DLIVSMSNLLRYSLQKLDKPVTLQDEIAHIQEYAAIQQARFRERIRFEMDFDPSVLQEEI 358 DLIVSMS L+RY+L +LD+PVTL DE+ H++EY IQQARFR R+ D SVL I Sbjct: 298 DLIVSMSKLIRYNLSQLDRPVTLGDELKHVREYFTIQQARFRNRVETRQQIDESVLDMLI 357 Query: 359 PALTLQPIVENAFLHGVANMESGAVISLVLRRTGGEVWIEISDNGVGMPEETLQSILRLE 418 PALT+QP+VENAF+HGV MESG +I LV+ R G V I I DNG GM E ++L L+ Sbjct: 358 PALTIQPLVENAFVHGVERMESGGIIQLVMERAGENVSIVIRDNGAGMTMEESNALLSLQ 417 Query: 419 AGAESG----KSTGLGTRNVLKRLQLFFGEEDLVRIHSRLGQGTTITIRIP 465 A+ S G GTRNV+KRL+LF+ D+V I S G+GT++ + +P Sbjct: 418 EPAQPAPAKKNSMGFGTRNVIKRLRLFYNRRDVVSIESEPGRGTSVILLLP 468 >B4BMS8.1 B4BMS8_9BACI Integral membrane sensor signal transduction histidine kinase Length = 481 Score = 290 bits (743), Expect = 1e-76, Method: Compositional matrix adjust. Identities = 178/481 (37%), Positives = 267/481 (55%), Gaps = 22/481 (4%) Query: 1 MTIRAKLLIGIPLLVVLANTIAFFVFQSGKVVQRSYDEMLGRILLIEQSTEWADTNLKLL 60 M IRAK+L+ + +VL N F ++Q+ + + YD+ + R+LL+ + ++ + L Sbjct: 1 MNIRAKMLVCFTVFLVLLNAAVFLLYQTSEEMMDEYDQRMRRLLLLNEVSQRVSHLTEQL 60 Query: 61 YAFLLDPRIDQAEMKSAGG---QLERLSGEIKQEGGQTDV---SFTMEDYISMTASFLEQ 114 A+ + + E K A Q L + +Q V E+Y M SFLE+ Sbjct: 61 NAY-----VSEKEGKYARAYEQQYRWLQQQRRQLAANLAVLSDRLAAENYEHMIESFLEE 115 Query: 115 KQAAIQASASEDPQIAFEHYVEAEKIVGYIREEGQQLVDAELAFYRPIIQNIREENDRMN 174 + + + ++ H E I +++EE L+D EL Y+ + N Sbjct: 116 AALTVYHFQAGNIKLYSSHLHETMNIASFLQEETLNLIDDELTAYQKQYDEVARRNRDFR 175 Query: 175 RLGAALFGMNALMGVLLAVWASRSITGPVGRLVGLAKRIATGNLDIGP--QPSRDDELGI 232 +G LF L GVLLAV+ S +T P+ L A+ I G LD GP P+ +DEL + Sbjct: 176 YMGMGLFVTTLLFGVLLAVFFSGRLTKPIILLSRAAQAIGAGRLD-GPDVNPTTNDELRL 234 Query: 233 LSNAIQQMSADLSVLIERDKKSLEMQRLVKELELQALQSQINPHFLFNTLNVLSRLALLE 292 L+ M +L LI K+ E+ RLVKELEL++LQSQINPHFLFNTLN ++++A LE Sbjct: 235 LTITFNDMRRNLKQLIAEMKQKAELDRLVKELELKSLQSQINPHFLFNTLNTVAKMAYLE 294 Query: 293 GADRTSDLIVSMSNLLRYSLQKLDKPVTLQDEIAHIQEYAAIQQARFRERIRFEMDFDPS 352 A +TS LI +++ +LRY+L L + VTL DE+ +EY IQQ RF +RI+F ++ +PS Sbjct: 295 DAPQTSRLIEAVAAILRYNLGDLQRTVTLADEVRVAREYFFIQQTRFFDRIKFSLEAEPS 354 Query: 353 VLQEEIPALTLQPIVENAFLHGVANMESGAVISLVLRRTGGEVWIEISDNGVGMPEET-- 410 L + +P LTLQP++ENAF+HG+ + E GA +++ + V +E+ DNGVGM E+ Sbjct: 355 CLGQRLPPLTLQPLIENAFIHGIESYEQGAELTVSIAEGESRVIVEVRDNGVGMDEQVKT 414 Query: 411 ------LQSILRLEAGAESGKSTGLGTRNVLKRLQLFFGEEDLVRIHSRLGQGTTITIRI 464 G STG+G RNV++RLQLF+G D+V I S G+GTTI +R+ Sbjct: 415 EFEAFIRGEERESRREEGRGHSTGIGLRNVIRRLQLFYGVTDVVDIESAPGRGTTIRLRL 474 Query: 465 P 465 P Sbjct: 475 P 475 >Q9K691.1 Q9K691_BACHD Two-component sensor histidine kinase Length = 481 Score = 284 bits (727), Expect = 1e-74, Method: Compositional matrix adjust. Identities = 167/481 (34%), Positives = 281/481 (58%), Gaps = 26/481 (5%) Query: 3 IRAKLLIGIPLLVVLANTIAFFVFQSGKVVQRSYDEMLGRILLIEQSTEWADTNLKLLYA 62 IR KLL +L+V+ +++F++ S + Y+E R L++ ++ + + L Sbjct: 4 IRTKLLAYFLVLLVILIAVSYFLYASNQRTLYQYNESFERFLVLNDISQTTNQIYETLNQ 63 Query: 63 FLLDPRID--------QAEMKSAGGQLERLSGEIKQEGGQTDVSFTMEDYISMTASFLEQ 114 ++ + D ++++ + Q+E+L I+ E + +++Y M SFLE+ Sbjct: 64 YVTEETPDLFEEYSEQRSKLITIQQQIEKL---IRNERN----AVHLKNYDHMVTSFLEK 116 Query: 115 KQAAIQASASEDPQI-AFEHYV-EAEKIVGYIREEGQQLVDAELAFYRPIIQNIREENDR 172 + A A ++ +I A+ Y+ E+++I YIRE L+D EL Y+ ++ +++ Sbjct: 117 TEQA--AEGFDEQRIDAYPFYLDESQQIAQYIREMTLTLIDDELTNYQQFYNSMLQKHSY 174 Query: 173 MNRLGAALFGMNALMGVLLAVWASRSITGPVGRLVGLAKRIATGNLD-IGPQPSRDDELG 231 +L ++F ++ A+W S IT P+ RL A I++G LD + S +DEL Sbjct: 175 TTQLAISIFVSILVLSSFFAIWLSDGITKPISRLTNAAYEISSGQLDGKDVKVSSNDELA 234 Query: 232 ILSNAIQQMSADLSVLIERDKKSLEMQRLVKELELQALQSQINPHFLFNTLNVLSRLALL 291 L+ M ++S L++ +++ + +L+KE+EL++LQSQINPHFLFNTLNV+ +++ + Sbjct: 235 FLTETFNDMRQNISGLVKEMEETSRLNQLLKEMELRSLQSQINPHFLFNTLNVILKMSYI 294 Query: 292 EGADRTSDLIVSMSNLLRYSLQKLDKPVTLQDEIAHIQEYAAIQQARFRERIRFEMDFDP 351 E A++T LI S+S+LLRY+L LD+ T+ DE +QEY I +ARF + I ++ D Sbjct: 295 EQAEKTGKLIASVSSLLRYNLGSLDRTTTVADEANMLQEYFHILKARFGDWISLSLNVDD 354 Query: 352 SVLQEEIPALTLQPIVENAFLHGVANMESGAVISLVLRRTGGEVWIEISDNGVGMPEETL 411 IP LTLQP+VENAF+HGV MESGA I + +++ G + I ISDNG GM E T Sbjct: 355 EARPYLIPCLTLQPLVENAFIHGVEQMESGAAIDVTIQKVGDSLTISISDNGAGMDEATR 414 Query: 412 QSIL------RLEAGAESGKSTGLGTRNVLKRLQLFFGEEDLVRIHSRLGQGTTITIRIP 465 + +L + E A G STG+G RNV++RL++F+ + +L+ I S LG+GTT+++ +P Sbjct: 415 KELLSSKEEDQTEKRASGGHSTGIGMRNVMRRLEIFYQKTELLTIESSLGEGTTVSLLLP 474 Query: 466 A 466 Sbjct: 475 T 475 >Q5KUZ2.1 Q5KUZ2_GEOKA Two-component sensor histidine kinase Length = 489 Score = 283 bits (724), Expect = 2e-74, Method: Compositional matrix adjust. Identities = 179/483 (37%), Positives = 273/483 (56%), Gaps = 16/483 (3%) Query: 1 MTIRAKLLIGIPLLVVLANTIAFFVFQSGKVVQRSYDEMLGRILLIEQSTEWADTNLKLL 60 M IR K+L+ + ++L N F ++Q+ + + YD+ + R+LL+ + ++ A+ ++ L Sbjct: 1 MNIRTKMLLCFTVFLLLLNGAVFLLYQTSEEMMSDYDQRMRRLLLLNEVSQRANRMMEQL 60 Query: 61 YAFLLDPRIDQAEMKSAGGQLERLSGEIKQEGGQTDV---SFTMEDYISMTASFLEQKQA 117 A++ + A ++ + L +Q G V E+Y M S LE+ Sbjct: 61 NAYVSEKEGRYA--RAYEREFRWLQQRRRQLGAILPVLSDRLAAENYEHMIESLLEEAAL 118 Query: 118 AIQASASEDPQIAFEHYVEAEKIVGYIREEGQQLVDAELAFYRPIIQNIREENDRMNRLG 177 + + + + H E I +++EE L+D EL Y+ + N +G Sbjct: 119 TVYHFQAGNIGLYSSHLHETMNIASFLQEETLNLIDDELTAYQRRYDEVERRNRYFRYMG 178 Query: 178 AALFGMNALMGVLLAVWASRSITGPVGRLVGLAKRIATGNLDIGP--QPSRDDELGILSN 235 LF L+G LLAV+ S +T P+ L A+ IA G LD GP +P +DEL +L+ Sbjct: 179 MGLFVTTLLLGALLAVFFSGHLTKPIILLSRAARSIADGRLD-GPDIEPMTNDELRLLTI 237 Query: 236 AIQQMSADLSVLIERDKKSLEMQRLVKELELQALQSQINPHFLFNTLNVLSRLALLEGAD 295 M +L LI K+ E+ RLVKELEL++LQSQINPHFLFNTLN ++++A LE A Sbjct: 238 TFNDMRRNLKQLIAEMKQKAELDRLVKELELKSLQSQINPHFLFNTLNTVAKMAYLEDAQ 297 Query: 296 RTSDLIVSMSNLLRYSLQKLDKPVTLQDEIAHIQEYAAIQQARFRERIRFEMDFDPSVLQ 355 +TS LI +++ +LRY+L L + VTL DE+ +EY IQQ RF +RI+F ++ +PS L Sbjct: 298 QTSRLIEAVAAILRYNLGDLQRTVTLADEVRIAREYFFIQQTRFFDRIKFSLEAEPSCLD 357 Query: 356 EEIPALTLQPIVENAFLHGVANMESGAVISLVLRRTGGEVWIEISDNGVGMPEETLQSIL 415 + IP LTLQP++ENAF+HG+ E GA +S+ + G V +E+ DNGVGM E+ + Sbjct: 358 QPIPPLTLQPLIENAFIHGIETYEQGAELSVSVFAENGRVVVEVRDNGVGMDEQVKAELE 417 Query: 416 RLEAGAE--------SGKSTGLGTRNVLKRLQLFFGEEDLVRIHSRLGQGTTITIRIPAG 467 L G E G STG+G NV++RLQLF+G D+ I S LG+GTT+ + +P Sbjct: 418 ALIRGEEPRTRREQGRGHSTGIGLHNVIRRLQLFYGVMDVAEIESALGKGTTVRLWLPRW 477 Query: 468 KGG 470 +GG Sbjct: 478 QGG 480 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.136 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,562,163,598 Number of extensions: 102986186 Number of successful extensions: 350666 Number of sequences better than 1.0e-05: 2388 Number of HSP's gapped: 348782 Number of HSP's successfully gapped: 2388 Length of query: 471 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 332 Effective length of database: 1,751,175,118 Effective search space: 581390139176 Effective search space used: 581390139176 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)