BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0350.0038.p (245 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KK03.1 B3KK03_9BACI Pyruvate formate-lyase activating enzyme E... 381 e-104 B1D6G6.1 B1D6G6_9BACL Pyruvate formate-lyase activating enzyme E... 293 7e-78 B9EA79.1 B9EA79_MACCJ Formate acetyltransferase activating enzyme 260 6e-68 C3BFE6.1 C3BFE6_9BACI Putative uncharacterized protein 255 2e-66 C3AZ00.1 C3AZ00_BACMY Putative uncharacterized protein 255 2e-66 >B3KK03.1 B3KK03_9BACI Pyruvate formate-lyase activating enzyme EC=1.97.1.4 Length = 241 Score = 381 bits (979), Expect = e-104, Method: Compositional matrix adjust. Identities = 181/237 (76%), Positives = 202/237 (85%) Query: 1 MANGHIHSLETFGTVDGPGIRFVLFMQGCLLKCQYCHNPDTWGLNEGREMSIEDVLAEIE 60 M GH+HS+ETFGTVDGPGIRFVLFMQGCLLKCQYCHNPDTWGL+EG EM+++DVL EIE Sbjct: 1 MLKGHVHSIETFGTVDGPGIRFVLFMQGCLLKCQYCHNPDTWGLHEGHEMTVDDVLREIE 60 Query: 61 PYLNYYRTSGGGLTVSGGEPTLQAHFVKQLFTEVKKRWNLHTTLDTNGYNEGDKINXXXX 120 PYL+YYR+SGGGLTVSGGEPTLQ FV +LF EVK+RWNLHTTLD+NGYNE DKI+ Sbjct: 61 PYLSYYRSSGGGLTVSGGEPTLQYPFVTELFKEVKRRWNLHTTLDSNGYNEPDKISELLE 120 Query: 121 XXXXXXXXXKHIDDEAHIKLTGKSNERTLKLARWLSDHNRKMWIRHVYVPGIHNREEDLL 180 KHI+DE HIKLTGKSNERTLK ARWLSDH R MWIRHVYVPGIH+ E+DL+ Sbjct: 121 HTDLVLLDLKHINDEKHIKLTGKSNERTLKTARWLSDHGRNMWIRHVYVPGIHDSEDDLV 180 Query: 181 NLGRFIGTLNGVEKFEILPYHQMGIYKWEVLGRPYELEGVPSPTEEEVQRAYRLIEE 237 LG+FI TL+GVEKFEILPYHQMG+YKW+ LG Y LEGVPSP+EEEV RAYRLI E Sbjct: 181 GLGQFIRTLHGVEKFEILPYHQMGVYKWKELGMAYPLEGVPSPSEEEVARAYRLIGE 237 >B1D6G6.1 B1D6G6_9BACL Pyruvate formate-lyase activating enzyme EC=1.97.1.4 Length = 244 Score = 293 bits (751), Expect = 7e-78, Method: Compositional matrix adjust. Identities = 132/234 (56%), Positives = 171/234 (73%) Query: 3 NGHIHSLETFGTVDGPGIRFVLFMQGCLLKCQYCHNPDTWGLNEGREMSIEDVLAEIEPY 62 G IHS++TFGTVDGPGIRFVLFMQGC L+CQ+CHNPDTW GR+++++D+L EIEPY Sbjct: 2 KGRIHSIDTFGTVDGPGIRFVLFMQGCALQCQFCHNPDTWDTAAGRQVTVDDILEEIEPY 61 Query: 63 LNYYRTSGGGLTVSGGEPTLQAHFVKQLFTEVKKRWNLHTTLDTNGYNEGDKINXXXXXX 122 L YYR SGGG+TV+GGEPTLQA FV LF K+++ LHT LD++G+ + + Sbjct: 62 LPYYRGSGGGITVTGGEPTLQAPFVAALFKACKEKYGLHTALDSSGFCDPSHASELMNDT 121 Query: 123 XXXXXXXKHIDDEAHIKLTGKSNERTLKLARWLSDHNRKMWIRHVYVPGIHNREEDLLNL 182 K ID + H +LT + N+R L A+WLS ++ +WIRHV +PGI + EDL L Sbjct: 122 DLVLLDLKQIDRDKHERLTSQPNDRILHFAKWLSSISKPVWIRHVLIPGITDHAEDLRLL 181 Query: 183 GRFIGTLNGVEKFEILPYHQMGIYKWEVLGRPYELEGVPSPTEEEVQRAYRLIE 236 GRFIG L VE+ E+LPYH+MG+YKW+ LGRPY LEGVP+PTE+EV RA +I+ Sbjct: 182 GRFIGGLVNVERLELLPYHRMGVYKWQTLGRPYPLEGVPAPTEQEVTRAMAIIQ 235 >B9EA79.1 B9EA79_MACCJ Formate acetyltransferase activating enzyme Length = 251 Score = 260 bits (665), Expect = 6e-68, Method: Compositional matrix adjust. Identities = 125/243 (51%), Positives = 169/243 (69%), Gaps = 8/243 (3%) Query: 1 MANGHIHSLETFGTVDGPGIRFVLFMQGCLLKCQYCHNPDTWGL-NEGREMSIEDVLAEI 59 M GHIHS+E+ GTVDGPG+R++LF QGCLL+CQ+CHNPDTW + RE++ E+++ EI Sbjct: 1 MIKGHIHSIESLGTVDGPGLRYILFTQGCLLRCQFCHNPDTWEIGTPSREVTAEEMVEEI 60 Query: 60 EPYLNYYRTSGGGLTVSGGEPTLQAHFVKQLFTEVKKRWNLHTTLDTNG--YNEGDKI-- 115 PY+ Y+ SGGG+T+SGGEP LQ F++QLF +K+ +HT +DT+ +NE Sbjct: 61 VPYIPYFNASGGGVTISGGEPLLQLPFIEQLFRRLKEE-GIHTCIDTSAGCFNETPAFMK 119 Query: 116 --NXXXXXXXXXXXXXKHIDDEAHIKLTGKSNERTLKLARWLSDHNRKMWIRHVYVPGIH 173 N KHID+E H+ LTGK N LK AR LSD + +WIRHV VPGI Sbjct: 120 HFNPVQENTDLFLLDIKHIDNEKHLSLTGKPNTHILKFARMLSDRKQPVWIRHVLVPGIT 179 Query: 174 NREEDLLNLGRFIGTLNGVEKFEILPYHQMGIYKWEVLGRPYELEGVPSPTEEEVQRAYR 233 + +EDL+ LG+FI +L VEKFEILPYHQ+G++KWE LG Y LEGV +P++E V++AY Sbjct: 180 DDKEDLIKLGQFINSLENVEKFEILPYHQLGVHKWEALGIKYPLEGVEAPSDETVRQAYD 239 Query: 234 LIE 236 ++ Sbjct: 240 YVD 242 >C3BFE6.1 C3BFE6_9BACI Putative uncharacterized protein Length = 243 Score = 255 bits (652), Expect = 2e-66, Method: Compositional matrix adjust. Identities = 121/242 (50%), Positives = 169/242 (69%), Gaps = 7/242 (2%) Query: 1 MANGHIHSLETFGTVDGPGIRFVLFMQGCLLKCQYCHNPDTWGLNEGREMSIEDVLAEIE 60 M G IHS+E+ GTVDGPGIR+V+F QGCLL+CQYCHN DTW + +G+E+++E+V+ ++ Sbjct: 1 MVKGRIHSVESCGTVDGPGIRYVIFTQGCLLRCQYCHNADTWEIGKGKEITVEEVMQDVT 60 Query: 61 PYLNYYRTSGGGLTVSGGEPTLQAHFVKQLFTEVKKRWNLHTTLDTNG--YNE----GDK 114 YL + SGGG+TVSGGEP LQ F+ +LF + K+ +HTT+D++G Y+E K Sbjct: 61 CYLPFIEASGGGITVSGGEPLLQLDFLIELFKKCKE-IGIHTTIDSSGGCYSEEPEFQRK 119 Query: 115 INXXXXXXXXXXXXXKHIDDEAHIKLTGKSNERTLKLARWLSDHNRKMWIRHVYVPGIHN 174 +N KHID + H KLTGK+NE L+ AR+LSD + +W+RHV VPG+ + Sbjct: 120 LNILMDYTDLVLLDLKHIDPKKHRKLTGKTNEHILQFARYLSDKQKPIWVRHVLVPGVTD 179 Query: 175 REEDLLNLGRFIGTLNGVEKFEILPYHQMGIYKWEVLGRPYELEGVPSPTEEEVQRAYRL 234 EEDL L FI +L+ V+K E+LPYH++G+YKWE LG Y LEGV PTE+ VQ+A + Sbjct: 180 SEEDLQRLSSFIQSLSNVKKVEVLPYHKLGVYKWEALGHKYPLEGVEPPTEKNVQKAKNI 239 Query: 235 IE 236 ++ Sbjct: 240 LQ 241 >C3AZ00.1 C3AZ00_BACMY Putative uncharacterized protein Length = 243 Score = 255 bits (652), Expect = 2e-66, Method: Compositional matrix adjust. Identities = 121/242 (50%), Positives = 169/242 (69%), Gaps = 7/242 (2%) Query: 1 MANGHIHSLETFGTVDGPGIRFVLFMQGCLLKCQYCHNPDTWGLNEGREMSIEDVLAEIE 60 M G IHS+E+ GTVDGPGIR+V+F QGCLL+CQYCHN DTW + +G+E+++E+V+ ++ Sbjct: 1 MVKGRIHSVESCGTVDGPGIRYVIFTQGCLLRCQYCHNADTWEIGKGKEITVEEVMQDVT 60 Query: 61 PYLNYYRTSGGGLTVSGGEPTLQAHFVKQLFTEVKKRWNLHTTLDTNG--YNE----GDK 114 YL + SGGG+TVSGGEP LQ F+ +LF + K+ +HTT+D++G Y+E K Sbjct: 61 CYLPFIEASGGGITVSGGEPLLQLDFLIELFKKCKE-IGIHTTIDSSGGCYSEEPEFQRK 119 Query: 115 INXXXXXXXXXXXXXKHIDDEAHIKLTGKSNERTLKLARWLSDHNRKMWIRHVYVPGIHN 174 +N KHID + H KLTGK+NE L+ AR+LSD + +W+RHV VPG+ + Sbjct: 120 LNILMDYTDLVLLDLKHIDPKKHRKLTGKTNEHILQFARYLSDKQKPIWVRHVLVPGVTD 179 Query: 175 REEDLLNLGRFIGTLNGVEKFEILPYHQMGIYKWEVLGRPYELEGVPSPTEEEVQRAYRL 234 EEDL L FI +L+ V+K E+LPYH++G+YKWE LG Y LEGV PTE+ VQ+A + Sbjct: 180 SEEDLQRLSSFIQSLSNVKKVEVLPYHKLGVYKWEALGHKYPLEGVEPPTEKNVQKAKNI 239 Query: 235 IE 236 ++ Sbjct: 240 LQ 241 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.139 0.433 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,562,453,307 Number of extensions: 65330436 Number of successful extensions: 141080 Number of sequences better than 1.0e-05: 1553 Number of HSP's gapped: 139015 Number of HSP's successfully gapped: 1559 Length of query: 245 Length of database: 3,060,696,203 Length adjustment: 132 Effective length of query: 113 Effective length of database: 1,817,122,223 Effective search space: 205334811199 Effective search space used: 205334811199 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)