BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0350.0027.p (267 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A4XIM5.1 A4XIM5_CALS8 Esterase/lipase-like protein 300 9e-80 B9MM64.1 B9MM64_ANATD Esterase/lipase-like protein 288 3e-76 A4A0F3.1 A4A0F3_9PLAN Endo-1,4-beta-xylanase B 288 5e-76 B3KFU8.1 B3KFU8_9BACI Esterase/lipase-like protein 286 9e-76 Q029V9.1 Q029V9_SOLUE Alpha/beta hydrolase 277 8e-73 >A4XIM5.1 A4XIM5_CALS8 Esterase/lipase-like protein Length = 265 Score = 300 bits (768), Expect = 9e-80, Method: Compositional matrix adjust. Identities = 142/261 (54%), Positives = 186/261 (71%), Gaps = 3/261 (1%) Query: 8 LWPEGAPGALGTSDEDCPAITPYLVE-GDKNAAVLVCPGGGYGMRADHEGGPVAEWLNTL 66 LW + P + P + PY++E G +N+ ++V PGGGY RA HE PVA+WLN++ Sbjct: 4 LWEKDVP-LFDPQNGFVPYLEPYILENGKQNSCIIVFPGGGYTHRASHEAEPVAKWLNSI 62 Query: 67 GISAFVLRYRVAPYGYPHALRDAQRALRTIRYRAEEYGIDPDRLGILGFSAGGHLASAAG 126 GISAFVL YRV+PY +P L DA+RA+R +RY A+++ I+P ++G+LGFSAGGHLAS G Sbjct: 63 GISAFVLHYRVSPYKHPVPLFDAKRAVRLVRYNAKKWNINPKKIGVLGFSAGGHLASTVG 122 Query: 127 VLFDAGDEEASEPLERKSSRPDLLILCYPVISMAEGVTHQGSKENQLGTDPDPELVKRLS 186 LFD GD+++++P+E S RPD +ILCYPVISMAE TH SK+ LG PDP LV LS Sbjct: 123 TLFDQGDKKSNDPVEMVSCRPDCMILCYPVISMAE-FTHDSSKKALLGDSPDPVLVWTLS 181 Query: 187 SELQVTAATPPAFLWHTSDDGAVPVENSLQFAAALSRHRIPFDLHVYAHGQHGLGLADGE 246 S+ VT TPP FLWHTSDD +V VENSL FA AL +H +PF+LH++ HG+HGLGLA Sbjct: 182 SQNMVTERTPPTFLWHTSDDSSVRVENSLLFAMALKKHNVPFELHIFPHGRHGLGLAKDT 241 Query: 247 PHTRSWTEACASWLALNGYTK 267 PH +WT+ C WL G+ + Sbjct: 242 PHIEAWTKLCEKWLESIGFIE 262 >B9MM64.1 B9MM64_ANATD Esterase/lipase-like protein Length = 267 Score = 288 bits (737), Expect = 3e-76, Method: Compositional matrix adjust. Identities = 132/237 (55%), Positives = 177/237 (74%), Gaps = 2/237 (0%) Query: 25 PAITPYLVEG-DKNAAVLVCPGGGYGMRADHEGGPVAEWLNTLGISAFVLRYRVAPYGYP 83 P + PY +E +K + ++V PGGGY RA HEG PV +WLN++GISAF+L YRV PY +P Sbjct: 22 PHLVPYTLESSNKLSCIIVFPGGGYTHRAQHEGEPVCKWLNSIGISAFLLNYRVLPYKHP 81 Query: 84 HALRDAQRALRTIRYRAEEYGIDPDRLGILGFSAGGHLASAAGVLFDAGDEEASEPLERK 143 L DA+R++R +RY ++++ IDP+R+G+LGFSAGGHLAS G FD+GD++ ++P+ER Sbjct: 82 APLLDAKRSIRLVRYFSKKWNIDPNRIGVLGFSAGGHLASLVGTHFDSGDKKNNDPVERV 141 Query: 144 SSRPDLLILCYPVISMAEGVTHQGSKENQLGTDPDPELVKRLSSELQVTAATPPAFLWHT 203 SSRPD ++LCYPVIS+AE H+GSK+ LG +PDP LV LSS V++ TPP FLWHT Sbjct: 142 SSRPDCMVLCYPVISLAE-YAHEGSKKALLGENPDPVLVWTLSSHNMVSSKTPPTFLWHT 200 Query: 204 SDDGAVPVENSLQFAAALSRHRIPFDLHVYAHGQHGLGLADGEPHTRSWTEACASWL 260 SDD +VPVENSL FA AL +H +PF+LH++ HG+HGLGLA + + WT C W Sbjct: 201 SDDSSVPVENSLLFAMALKKHGVPFELHIFPHGRHGLGLASETLYVKEWTNLCEKWF 257 >A4A0F3.1 A4A0F3_9PLAN Endo-1,4-beta-xylanase B Length = 284 Score = 288 bits (736), Expect = 5e-76, Method: Compositional matrix adjust. Identities = 141/266 (53%), Positives = 191/266 (71%), Gaps = 5/266 (1%) Query: 4 KTMLLWPEGAPGALGTSDEDCPAITPYLVEG-DK-NAAVLVCPGGGYGMRAD-HEGGPVA 60 K LLWP GAP A G +D D P IT YL +G DK N V+V PGGGYG A HEG +A Sbjct: 9 KRELLWPAGAPDAKGDTDNDKPTITVYLADGADKTNCGVVVLPGGGYGHLATGHEGVDIA 68 Query: 61 EWLNTLGISAFVLRYRVAPYGYPHA--LRDAQRALRTIRYRAEEYGIDPDRLGILGFSAG 118 +W N+ G+SAFV+ YR GY H ++DAQRA+RT+R RAEE+G+ PD++G++GFSAG Sbjct: 69 KWYNSFGVSAFVVEYRHRGRGYAHPAPIQDAQRAIRTVRARAEEFGVSPDKIGVMGFSAG 128 Query: 119 GHLASAAGVLFDAGDEEASEPLERKSSRPDLLILCYPVISMAEGVTHQGSKENQLGTDPD 178 GHLAS A FDAGD +A++P+++ S RPD ILCYPVI++ + TH+GS++N LG +PD Sbjct: 129 GHLASTAATHFDAGDAKAADPIDQVSCRPDFAILCYPVIALDQPFTHRGSQKNLLGENPD 188 Query: 179 PELVKRLSSELQVTAATPPAFLWHTSDDGAVPVENSLQFAAALSRHRIPFDLHVYAHGQH 238 LVK LS+E QVT+ TPP FL+HTS+D VP +NS+ F AL + ++P ++H++A G+H Sbjct: 189 KALVKSLSNESQVTSDTPPTFLFHTSEDTGVPPQNSIVFYEALQKAKVPAEMHIFAKGRH 248 Query: 239 GLGLADGEPHTRSWTEACASWLALNG 264 G+GLA P T +W + C +WL+ G Sbjct: 249 GVGLAANIPGTSAWPKLCEAWLSGQG 274 >B3KFU8.1 B3KFU8_9BACI Esterase/lipase-like protein Length = 269 Score = 286 bits (733), Expect = 9e-76, Method: Compositional matrix adjust. Identities = 140/261 (53%), Positives = 184/261 (70%), Gaps = 2/261 (0%) Query: 6 MLLWPEGAPGALGTSDEDCPAITPYLVEGDK-NAAVLVCPGGGYGMRADHEGGPVAEWLN 64 M LW + PG + P +TPYL++ + + AVLV PGGGYG RADHEG PVA WLN Sbjct: 1 MKLWEQDTPGYASVEESFTPYMTPYLLQKETPHGAVLVLPGGGYGRRADHEGDPVALWLN 60 Query: 65 TLGISAFVLRYRVAPYGYPHALRDAQRALRTIRYRAEEYGIDPDRLGILGFSAGGHLASA 124 ++G+SAFV+ YRVAPY +P+ L DAQRA+R +R+ A+E+ IDP+R+GILGFSAGGHLA++ Sbjct: 61 SIGLSAFVVHYRVAPYRHPYPLYDAQRAIRLVRHHAKEWNIDPERIGILGFSAGGHLAAS 120 Query: 125 AGVLFDAGDEEASEPLERKSSRPDLLILCYPVISMAEGVTHQGSKENQLGTDPDPELVKR 184 A FD G+ EA + +ER+ RP+ ILCYPVIS + H+GS N LG +P +L Sbjct: 121 ASTYFDEGNPEAEDRVERQGCRPNASILCYPVISFKD-YYHEGSMINLLGENPAEDLRNG 179 Query: 185 LSSELQVTAATPPAFLWHTSDDGAVPVENSLQFAAALSRHRIPFDLHVYAHGQHGLGLAD 244 +S E V TPP+FLWHT+DDGAVPVENSL +A +LS+ +IPF+LHV+ G+HGLGLA+ Sbjct: 180 MSLEWAVKPDTPPSFLWHTADDGAVPVENSLMYAGSLSKQQIPFELHVFPEGRHGLGLAE 239 Query: 245 GEPHTRSWTEACASWLALNGY 265 + WT C WL G+ Sbjct: 240 EDASVSQWTRLCEGWLQKIGF 260 >Q029V9.1 Q029V9_SOLUE Alpha/beta hydrolase Length = 295 Score = 277 bits (708), Expect = 8e-73, Method: Compositional matrix adjust. Identities = 138/257 (53%), Positives = 183/257 (71%), Gaps = 5/257 (1%) Query: 8 LWPEGAPGALGTSDEDCPAITPYLVEGDKNA--AVLVCPGGGY-GMRADHEGGPVAEWLN 64 LW +GAPGALG+ + D P + PY+V + AV+V PGGGY G+ D EG +A+WLN Sbjct: 29 LWAQGAPGALGSDEIDKPTLAPYVVPTGRGTGTAVIVFPGGGYSGLSMDKEGDQIAKWLN 88 Query: 65 TLGISAFVLRYRVAP-YGYPHALRDAQRALRTIRYRAEEYGIDPDRLGILGFSAGGHLAS 123 +LG++AFVL+YR+ P Y +P L DAQRA+RT+R +A EY + PDR+G++GFSAGGHLAS Sbjct: 89 SLGVTAFVLKYRLGPKYHHPVELGDAQRAIRTVRAKAAEYRVMPDRVGVMGFSAGGHLAS 148 Query: 124 AAGVLFDAGDEEASEPLERKSSRPDLLILCYPVISMAEGVTHQGSKENQLGTDPDPELVK 183 AG FDAG+ EA++ ++R SSRPD L+LCYPV+S V HQGSK N LG +PDP+LV+ Sbjct: 149 TAGTHFDAGNPEAADAIDRVSSRPDFLVLCYPVVSFLNYV-HQGSKRNLLGENPDPKLVE 207 Query: 184 RLSSELQVTAATPPAFLWHTSDDGAVPVENSLQFAAALSRHRIPFDLHVYAHGQHGLGLA 243 +S+E QVTA TPP FL+HT+ D VP ENS+ F AL + +P +LH+Y G HG+GLA Sbjct: 208 SMSNETQVTAQTPPTFLFHTNADTGVPAENSVLFYMALRKAGVPAELHIYEKGPHGVGLA 267 Query: 244 DGEPHTRSWTEACASWL 260 + +W A WL Sbjct: 268 QTDEALSTWPSRLADWL 284 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.136 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,840,915,260 Number of extensions: 80921281 Number of successful extensions: 193032 Number of sequences better than 1.0e-05: 572 Number of HSP's gapped: 192346 Number of HSP's successfully gapped: 574 Length of query: 267 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 134 Effective length of database: 1,807,701,208 Effective search space: 242231961872 Effective search space used: 242231961872 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 130 (54.7 bits)