BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0350.0024.p (479 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A9KSL6.1 A9KSL6_CLOPH Kojibiose phosphorylase EC=2.4.1.230 592 e-167 Q3Y2T1.1 Q3Y2T1_ENTFC Glycoside hydrolase, family 65, C-terminal... 382 e-104 Q84IX5.1 Q84IX5_9BACI Maltose phosphorylase 379 e-103 Q9K109.1 Q9K109_NEIMB Maltose phosphorylase EC=2.4.1.8 377 e-102 C4VGM7.1 C4VGM7_ENTFA Glycosyl hydrolase, family 65 374 e-101 >A9KSL6.1 A9KSL6_CLOPH Kojibiose phosphorylase EC=2.4.1.230 Length = 805 Score = 592 bits (1526), Expect = e-167, Method: Compositional matrix adjust. Identities = 272/473 (57%), Positives = 350/473 (73%), Gaps = 1/473 (0%) Query: 1 VFADQSAYWSGIWETSDIRIEGDPENQQGIRFCIFQLYQTYHGDHPGYNIGAKGLTGEAY 60 + A+ +W W+ SDI IEGD NQQGIR+CIFQ++QTYHG G NIGAKGLTGEAY Sbjct: 332 LLAESIMWWKEKWKESDIVIEGDELNQQGIRYCIFQMHQTYHGTEAGTNIGAKGLTGEAY 391 Query: 61 RGLAFWDTESYCLPFYMFNNPKAAKNLLEFRYKTLPEAVDRAKEVDCEGACYPIATIDGT 120 G AFWDTE+YCLPFY+FNN KAAKNLL FRY+TL EA RAKE+DC+GA YPIATI G Sbjct: 392 SGNAFWDTETYCLPFYIFNNLKAAKNLLMFRYETLEEAKLRAKELDCKGAFYPIATISGR 451 Query: 121 ESCDLWQHSNLQLHVGTAVSYGIWHYVKNTGDRDFLYSKGAEMLIQISRFYATRGQWGQR 180 E C LWQHS+LQL T V+YGIW YVK T D +FL+ G MLI+ISR TRG + Sbjct: 452 ECCSLWQHSSLQLQASTGVAYGIWSYVKLTNDVEFLHQYGIPMLIEISRMLVTRGDYNAD 511 Query: 181 SGEYGYFGVMGPDEFQLMVNNNCYINFMAKKMFEFTLETLAGMEVQVPDAYAELIRKTGL 240 +YGY+GVMGPDEFQ+MVN+NCY N + K +FT + + ++ + + E++ + + Sbjct: 512 GTKYGYYGVMGPDEFQMMVNHNCYTNVLGKLTLKFTYDVMKQLKEEGL-SEQEIVNRYQV 570 Query: 241 RAEELTDWSSKAEHMRIPLDPDSGVYEEHDGYFELPHIDIHSIPVTDFPLYSNWSYDRLY 300 +EE+ D+ KA+ M IP D+ ++E+H GYF+LPH+D+ +IP+ +FPLY +W+YDR+Y Sbjct: 571 SSEEIEDFKKKADDMWIPYHADTLLFEQHQGYFDLPHVDVDAIPIEEFPLYHHWTYDRIY 630 Query: 301 RYDMIKQPDVLMFMFLYNGEFSREAKRANYEYYEPRCIHESSLSPSIHSILACEIGKEEE 360 R DMIKQPDVLM M L+N F+ + +ANYE+YEPRCIHESSLSPS+HSILA ++ K +E Sbjct: 631 RNDMIKQPDVLMMMLLFNSNFTIDQLKANYEFYEPRCIHESSLSPSVHSILASQLKKHKE 690 Query: 361 AYQFFEFATRLDLDNYNRNTREGLHTTSIAAAWMNIVYGFGGMRSDGERLSFRPTLPQRW 420 AYQFF FATR+DLDNYNRNT EGLHTTSIAAAWMNIVYGFGGMR+DGE LSF P++P+ W Sbjct: 691 AYQFFGFATRMDLDNYNRNTDEGLHTTSIAAAWMNIVYGFGGMRADGELLSFTPSIPETW 750 Query: 421 TSYSFQVMYEGVLLGITVDSERVRFKALRGGTASILVYGQQIMVDAAGTVLPL 473 TSYSF++ Y G ++ + V + F+ L G + +Y + +D + L + Sbjct: 751 TSYSFRIGYRGNVILVEVSKKEATFQVLEGSDVLVKIYENEYTLDQSKLKLEI 803 >Q3Y2T1.1 Q3Y2T1_ENTFC Glycoside hydrolase, family 65, C-terminal:Glycoside hydrolase, family 65, central catalytic:Glycoside hydrolase, family 65, N-terminal Length = 764 Score = 382 bits (980), Expect = e-104, Method: Compositional matrix adjust. Identities = 187/458 (40%), Positives = 288/458 (62%), Gaps = 10/458 (2%) Query: 4 DQSAYWSGIWETSDIRIEGDPENQQGIRFCIFQLYQTYHGDHPGYNIGAKGLTGEAYRGL 63 +Q+A W+ W+ +D+ IEGD E QQGIRF +FQL+ TY+G+ NIG KG TGE Y G Sbjct: 297 EQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTYYGEDERLNIGPKGFTGEKYGGA 356 Query: 64 AFWDTESYCLPFYM-FNNPKAAKNLLEFRYKTLPEAVDRAKEVDCEGACYPIATIDGTES 122 +WDTE+Y +P Y+ P+ KNLL++R+ LP+A+ A++ +GA YP+ T G E Sbjct: 357 TYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQAIHNAQQQGLKGALYPMVTFTGVEC 416 Query: 123 CDLWQHSNLQLHVGTAVSYGIWHYVKNTGDRDFLYSKGAEMLIQISRFYATRGQWGQRSG 182 + W+ + ++H A++Y I++Y TGD ++L ++G E+L +I+RF+A R + +R+ Sbjct: 417 HNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYLKNEGLEVLAEIARFWADRVHFSKRAN 476 Query: 183 EYGYFGVMGPDEFQLMVNNNCYINFMAKKMFEFTLETLAGMEVQVPDAYAELIRKTGLRA 242 +Y GV GP+E++ +NNN Y N +A + +T E + + +E Sbjct: 477 KYMIHGVTGPNEYENNINNNWYTNTIAAWVLRYTRENYLTYQKEAAITISE--------- 527 Query: 243 EELTDWSSKAEHMRIPLDPDSGVYEEHDGYFELPHIDIHSIPVTDFPLYSNWSYDRLYRY 302 EEL W AE+M P+D + G++ +HD + + + + IP +D PL +WS+D++ R Sbjct: 528 EELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVSDIPTSDLPLNQHWSWDKILRS 587 Query: 303 DMIKQPDVLMFMFLYNGEFSREAKRANYEYYEPRCIHESSLSPSIHSILACEIGKEEEAY 362 IKQ DVL ++ +N F++E K+ N+++YEP +HESSLSPSIHSILA E+G E +A Sbjct: 588 CFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVHESSLSPSIHSILAAELGMESKAV 647 Query: 363 QFFEFATRLDLDNYNRNTREGLHTTSIAAAWMNIVYGFGGMRSDGERLSFRPTLPQRWTS 422 + ++ RLDLDNYN +T +GLH TS+ +W++IV GF M++ ++L F P LP+ W + Sbjct: 648 EMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIVQGFAQMKTAHDQLRFAPFLPKTWKA 707 Query: 423 YSFQVMYEGVLLGITVDSERVRFKALRGGTASILVYGQ 460 YSF + Y G LL I+V E+V F+ L G I VY Q Sbjct: 708 YSFHINYRGRLLFISVTDEQVTFELLEGSPLEIYVYEQ 745 >Q84IX5.1 Q84IX5_9BACI Maltose phosphorylase Length = 758 Score = 379 bits (972), Expect = e-103, Method: Compositional matrix adjust. Identities = 190/465 (40%), Positives = 290/465 (62%), Gaps = 12/465 (2%) Query: 1 VFADQSAYWSGIWETSDIRIEGDPENQQGIRFCIFQLYQTYHGDHPGYNIGAKGLTGEAY 60 +F + +A W W+ +D+RI GDPE QQGIRF +FQL+ TY+GD P NIG KG TGE Y Sbjct: 292 LFQEHAAAWQKRWDKADVRIVGDPEAQQGIRFNLFQLFSTYYGDDPRLNIGPKGFTGEKY 351 Query: 61 RGLAFWDTESYCLPFYM-FNNPKAAKNLLEFRYKTLPEAVDRAKEVDCEGACYPIATIDG 119 G +WDTE+Y +P Y+ +P+ +NLL +RY LP+A + AK++ +GA YP+ T G Sbjct: 352 GGATYWDTEAYAVPMYLSVASPEVTRNLLLYRYNHLPQAYENAKKLGLKGALYPMVTFTG 411 Query: 120 TESCDLWQHSNLQLHVGTAVSYGIWHYVKNTGDRDFLYSKGAEMLIQISRFYATRGQWGQ 179 E + W+ + ++H A++Y I++YV TGD++++ G ++L+ ISRF+A R + + Sbjct: 412 IECHNEWEITFEEIHRNGAIAYAIYNYVNYTGDKEYINQYGIDVLVGISRFFADRVHFSE 471 Query: 180 RSGEYGYFGVMGPDEFQLMVNNNCYINFMAKKMFEFTLETLAGMEVQVPDAYAELIRKTG 239 R +Y GV GP+E++ VNNN Y N +A +FT+ +L + +KT Sbjct: 472 RKQKYMIHGVTGPNEYENNVNNNWYTNRIAVWTLKFTIASLDKIGKD---------KKTA 522 Query: 240 LRA--EELTDWSSKAEHMRIPLDPDSGVYEEHDGYFELPHIDIHSIPVTDFPLYSNWSYD 297 L +ELT W + M P D G++ + DG+ + P + +IP D PL +WS+D Sbjct: 523 LNITEKELTQWRDIIDRMYFPYDEKLGIFLQQDGFLDKPIQPVSAIPREDLPLNQHWSWD 582 Query: 298 RLYRYDMIKQPDVLMFMFLYNGEFSREAKRANYEYYEPRCIHESSLSPSIHSILACEIGK 357 R+ R IKQ DVL + +N EF+ E K+ ++E+YEP +HESSLSP +H+ILA E+ K Sbjct: 583 RILRSCYIKQADVLQGFYFFNDEFTMEEKQRHFEFYEPLTVHESSLSPCVHAILAAELRK 642 Query: 358 EEEAYQFFEFATRLDLDNYNRNTREGLHTTSIAAAWMNIVYGFGGMRSDGERLSFRPTLP 417 E++A + ++ +RLDLDNYN +T +GLH TS+ +W+ IV+GF GMR+ E LSF P LP Sbjct: 643 EDKAVELYKRTSRLDLDNYNHDTEDGLHITSMTGSWLAIVHGFAGMRTFNETLSFAPFLP 702 Query: 418 QRWTSYSFQVMYEGVLLGITVDSERVRFKALRGGTASILVYGQQI 462 + W +Y+F++ Y LL + V+ + V L G + ++G+ I Sbjct: 703 KIWDAYAFKINYRNRLLEVKVNKKHVTVSLLEGEPLELKLFGEPI 747 >Q9K109.1 Q9K109_NEIMB Maltose phosphorylase EC=2.4.1.8 Length = 752 Score = 377 bits (969), Expect = e-102, Method: Compositional matrix adjust. Identities = 192/467 (41%), Positives = 286/467 (61%), Gaps = 11/467 (2%) Query: 5 QSAYWSGIWETSDIRIEGDPENQQGIRFCIFQLYQTYHGDHPGYNIGAKGLTGEAYRGLA 64 A W WE +D+ IEG E QQGIRF +FQL+ TY+G+ NIG KG TGE Y G Sbjct: 292 HKAGWLHRWEIADVVIEGSDEAQQGIRFNLFQLFSTYYGEDARLNIGPKGFTGEKYGGAT 351 Query: 65 FWDTESYCLPFYM-FNNPKAAKNLLEFRYKTLPEAVDRAKEVDCEGACYPIATIDGTESC 123 +WDTE+Y +P Y+ P+ +NLL++R LP+A A+E GA YP+ T G E Sbjct: 352 YWDTEAYAVPLYLALAEPEVTRNLLQYRRNQLPQAQHNAREQGLAGALYPMVTFTGIECH 411 Query: 124 DLWQHSNLQLHVGTAVSYGIWHYVKNTGDRDFLYSKGAEMLIQISRFYATRGQWGQRSGE 183 + W+ + ++H A+ Y I++Y TGD +L +G E+L+++SRF+A R + +R+G+ Sbjct: 412 NEWEITFEEIHRNGAIPYAIYNYTNYTGDEGYLAKEGLEVLVEVSRFWADRVHFSKRNGK 471 Query: 184 YGYFGVMGPDEFQLMVNNNCYINFMAKKMFEFTLETLAGMEVQVPDAYAELIRKTGLRAE 243 Y GV GP+E++ +NNN Y N +A + ++T E LA + PD +RA+ Sbjct: 472 YMIHGVTGPNEYENNINNNWYTNTLAAWVLDYTREALA--KYPRPD--------LNVRAD 521 Query: 244 ELTDWSSKAEHMRIPLDPDSGVYEEHDGYFELPHIDIHSIPVTDFPLYSNWSYDRLYRYD 303 EL W+ + +M P D + GV+ +HDG+ + + ++ D PL WS+D++ R Sbjct: 522 ELEKWADISANMYRPHDEELGVFVQHDGFLDKDIRPVSALSPDDLPLNQKWSWDKILRSP 581 Query: 304 MIKQPDVLMFMFLYNGEFSREAKRANYEYYEPRCIHESSLSPSIHSILACEIGKEEEAYQ 363 IKQ DVL ++ ++ F+ + KR N+++YEP +HESSLSP IHSILA E+GKEE+A + Sbjct: 582 FIKQADVLQGIYFFSDRFNIDEKRRNFDFYEPMTVHESSLSPCIHSILAAELGKEEKAVE 641 Query: 364 FFEFATRLDLDNYNRNTREGLHTTSIAAAWMNIVYGFGGMRSDGERLSFRPTLPQRWTSY 423 ++ RLDLDNYN +T +GLH TS+ +W+ IV GF M++ G +LSF P LP WT Y Sbjct: 642 MYQRTARLDLDNYNNDTEDGLHITSMTGSWLAIVQGFAQMKTWGGKLSFAPFLPSAWTGY 701 Query: 424 SFQVMYEGVLLGITVDSERVRFKALRGGTASILVYGQQIMVDAAGTV 470 +F + Y G L+ + V E V F L+G + + VYG+ I +D + TV Sbjct: 702 AFHINYRGRLIKVAVGKENVVFTLLKGESLDLQVYGKDITLDGSHTV 748 >C4VGM7.1 C4VGM7_ENTFA Glycosyl hydrolase, family 65 Length = 764 Score = 374 bits (960), Expect = e-101, Method: Compositional matrix adjust. Identities = 187/464 (40%), Positives = 287/464 (61%), Gaps = 15/464 (3%) Query: 13 WETSDIRIEGDPENQQGIRFCIFQLYQTYHGDHPGYNIGAKGLTGEAYRGLAFWDTESYC 72 W+ +D+ IEGD E QQGIRF +FQL+ TY+G+ NIG KG TGE Y G +WDTE+Y Sbjct: 306 WQLADVVIEGDDEAQQGIRFNLFQLFSTYYGEDDRLNIGPKGFTGEKYGGATYWDTEAYA 365 Query: 73 LPFYM-FNNPKAAKNLLEFRYKTLPEAVDRAKEVDCEGACYPIATIDGTESCDLWQHSNL 131 +P Y+ P+ KNLL++R+ LP+A+ A++ +GA YP+ T G E + W+ + Sbjct: 366 VPLYLALAKPEVTKNLLKYRHNQLPQAIHNAQQQGLKGALYPMVTFTGVECHNEWEITFE 425 Query: 132 QLHVGTAVSYGIWHYVKNTGDRDFLYSKGAEMLIQISRFYATRGQWGQRSGEYGYFGVMG 191 ++H A++Y I++YV TGD D+L G E+L+ I+RF+A R + QR +Y GV G Sbjct: 426 EIHRNGAIAYAIYNYVNYTGDEDYLKDAGLEVLVAIARFWADRVHFSQRHKQYMIHGVTG 485 Query: 192 PDEFQLMVNNNCYINFMAKKMFEFTLETLAGMEVQVPDAYAELIRKTGLRA--EELTDWS 249 P+E++ +NNN Y N +A + +T E +Y + +T L+ +EL W+ Sbjct: 486 PNEYENNINNNWYTNTIAAWVLRYTRE-----------SYLKFQEETTLKIADDELAKWA 534 Query: 250 SKAEHMRIPLDPDSGVYEEHDGYFELPHIDIHSIPVTDFPLYSNWSYDRLYRYDMIKQPD 309 E+M P+D + G++ +HD + + + + +P+++ PL +WS+D++ R IKQ D Sbjct: 535 DIVENMYFPVDNELGIFVQHDTFLDKDLMPVSDLPLSELPLNQHWSWDKILRSCFIKQAD 594 Query: 310 VLMFMFLYNGEFSREAKRANYEYYEPRCIHESSLSPSIHSILACEIGKEEEAYQFFEFAT 369 VL ++ +N FS E KR N+ +YEP +HESSLSPSIH++LA E+G EE+A + ++ Sbjct: 595 VLQGIYFFNDAFSLEEKRRNFNFYEPMTVHESSLSPSIHAVLAAELGMEEKAVEMYQRTA 654 Query: 370 RLDLDNYNRNTREGLHTTSIAAAWMNIVYGFGGMRSDGERLSFRPTLPQRWTSYSFQVMY 429 RLDLDNYN +T +GLH TS+ +W+ IV GF M++D ++L F P LP WT+YSF + Y Sbjct: 655 RLDLDNYNNDTEDGLHITSMTGSWLAIVQGFAQMKTDHQQLKFAPFLPATWTAYSFHINY 714 Query: 430 EGVLLGITVDSERVRFKALRGGTASILVYGQQIMVDAAGTVLPL 473 LL + V +++V F L G + VY Q+ + VLP+ Sbjct: 715 RNRLLFVEVSADQVAFTLLDGPAIPLTVYDQKYTLKDR-LVLPI 757 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.138 0.434 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,117,079,150 Number of extensions: 138624462 Number of successful extensions: 262915 Number of sequences better than 1.0e-05: 515 Number of HSP's gapped: 261392 Number of HSP's successfully gapped: 518 Length of query: 479 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 339 Effective length of database: 1,741,754,103 Effective search space: 590454640917 Effective search space used: 590454640917 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)