BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0350.0022.p (189 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A9KSL6.1 A9KSL6_CLOPH Kojibiose phosphorylase EC=2.4.1.230 191 2e-47 C1S5P7.1 C1S5P7_9SPHI Maltose phosphorylase EC=2.4.1.8 122 1e-26 B3K4G4.1 B3K4G4_9BACI Kojibiose phosphorylase EC=2.4.1.230 119 1e-25 Q50LH0.1 Q50LH0_9BACL Maltose phosphorylase 119 1e-25 B8CY36.1 B8CY36_HALOH Kojibiose phosphorylase EC=2.4.1.230 113 8e-24 >A9KSL6.1 A9KSL6_CLOPH Kojibiose phosphorylase EC=2.4.1.230 Length = 805 Score = 191 bits (486), Expect = 2e-47, Method: Compositional matrix adjust. Identities = 88/163 (53%), Positives = 124/163 (76%), Gaps = 1/163 (0%) Query: 15 MAKVADKYLKVDPWAVIEEGFDPLRNRTSESIFSVGNEYMGVRGYAEEGYSGDSLPGSYF 74 MAK+AD Y K DP+ +IEEGFDP + SESIFS+GNEYMGVRGY EEGYS DSL GSYF Sbjct: 1 MAKIADLYYKADPFQIIEEGFDPNYAKVSESIFSLGNEYMGVRGYFEEGYSSDSLVGSYF 60 Query: 75 NGLHEQLEIGN-HYKGMIRSLRYMVNAVDWLHTRLTVEGEQLDLAHSRISDYSRRLDFRT 133 NG++E+ E+ + +YKG+I +MVN+VD+ +T+++++GEQLDLA +I+ + R LD +T Sbjct: 61 NGIYERAEVSSQNYKGIIDYTEFMVNSVDYFYTKVSLDGEQLDLAKVKINQFRRYLDLKT 120 Query: 134 GTYSRQFIWLLGSGKKLKLTFTRLVSMTDSHLGLQQVVFEPLN 176 G R FIW L +GK++++ F R +SM+D+ + Q++ F PLN Sbjct: 121 GVLKRSFIWELQNGKQIEIMFERFLSMSDACMAGQRIRFLPLN 163 >C1S5P7.1 C1S5P7_9SPHI Maltose phosphorylase EC=2.4.1.8 Length = 774 Score = 122 bits (307), Expect = 1e-26, Method: Composition-based stats. Identities = 64/158 (40%), Positives = 95/158 (60%), Gaps = 2/158 (1%) Query: 21 KYLKVDPWAVIEEGFDPLRNRTSESIFSVGNEYMGVRGYAEEGYSGDSLPGSYFNGLH-- 78 +Y+KVD W +IEEGF P N+ SESIFS+GN MG R EE YSG++L G+Y G++ Sbjct: 3 QYIKVDGWNIIEEGFVPHYNKISESIFSLGNGRMGQRANFEETYSGETLQGNYVAGVYYP 62 Query: 79 EQLEIGNHYKGMIRSLRYMVNAVDWLHTRLTVEGEQLDLAHSRISDYSRRLDFRTGTYSR 138 ++ +G G ++NA +W+ + +E E+LDL +R++D+SR L+ + G R Sbjct: 63 DKTRVGWWKNGYPEYFAKVLNAANWIGIGIRIEEEELDLHKARVTDFSRVLNMQEGYLQR 122 Query: 139 QFIWLLGSGKKLKLTFTRLVSMTDSHLGLQQVVFEPLN 176 F L SGK++K+T TR S+ D G + PLN Sbjct: 123 SFTATLESGKQVKVTATRFCSIVDDETGAIRYSITPLN 160 >B3K4G4.1 B3K4G4_9BACI Kojibiose phosphorylase EC=2.4.1.230 Length = 767 Score = 119 bits (298), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 60/158 (37%), Positives = 93/158 (58%), Gaps = 2/158 (1%) Query: 21 KYLKVDPWAVIEEGFDPLRNRTSESIFSVGNEYMGVRGYAEEGYSGDSLPGSYFNGLH-- 78 +YLK+D W++IEEGFDP + SESIFS+GN +MG R EE YSG SL GSY G++ Sbjct: 3 QYLKLDEWSIIEEGFDPHTHEISESIFSIGNGFMGQRANFEESYSGSSLQGSYMAGVYYP 62 Query: 79 EQLEIGNHYKGMIRSLRYMVNAVDWLHTRLTVEGEQLDLAHSRISDYSRRLDFRTGTYSR 138 ++ +G G ++N+ +W+ + ++G LDLA + D+ R L+ + G SR Sbjct: 63 DKTRVGWWKNGYPEYFAKVLNSTNWIGIGIEIDGTPLDLAKCTVKDFVRELNMKEGFLSR 122 Query: 139 QFIWLLGSGKKLKLTFTRLVSMTDSHLGLQQVVFEPLN 176 +F ++ GK+L++ R VS+ +G + PLN Sbjct: 123 RFTVVMEDGKELQIEAVRFVSIVRHEIGAIRYAVTPLN 160 >Q50LH0.1 Q50LH0_9BACL Maltose phosphorylase Length = 768 Score = 119 bits (298), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 60/158 (37%), Positives = 92/158 (58%), Gaps = 2/158 (1%) Query: 21 KYLKVDPWAVIEEGFDPLRNRTSESIFSVGNEYMGVRGYAEEGYSGDSLPGSYFNGLH-- 78 +YLK+D W++IEEGFDP + SES+FS+GN +MG R EE YSG SL GSY G++ Sbjct: 3 QYLKLDEWSIIEEGFDPHTHEISESVFSIGNGFMGQRANFEEAYSGSSLQGSYMAGVYYP 62 Query: 79 EQLEIGNHYKGMIRSLRYMVNAVDWLHTRLTVEGEQLDLAHSRISDYSRRLDFRTGTYSR 138 ++ +G G ++N+ +W+ + ++G LDLA + D+ R L+ + G SR Sbjct: 63 DKTRVGWWKNGYPEYFAKVLNSTNWIGIDIQIDGTPLDLAKCTVKDFVRELNMKEGFLSR 122 Query: 139 QFIWLLGSGKKLKLTFTRLVSMTDSHLGLQQVVFEPLN 176 +F + GK+LK+ R VS+ +G + PLN Sbjct: 123 RFTAVTEDGKELKVEAIRFVSIVRHEIGAIRYAVTPLN 160 >B8CY36.1 B8CY36_HALOH Kojibiose phosphorylase EC=2.4.1.230 Length = 778 Score = 113 bits (283), Expect = 8e-24, Method: Compositional matrix adjust. Identities = 57/158 (36%), Positives = 89/158 (56%), Gaps = 5/158 (3%) Query: 24 KVDPWAVIEEGFDPLRNRTSESIFSVGNEYMGVRGYAEEGYSGD---SLPGSYFNGLH-- 78 K +PW + E+ F N +E+IF++GN YMG+RG EE YSG + PG+Y NG++ Sbjct: 16 KYEPWTITEKEFKIENNHHNETIFALGNGYMGLRGTLEEDYSGPEQTTTPGTYINGVYTE 75 Query: 79 EQLEIGNHYKGMIRSLRYMVNAVDWLHTRLTVEGEQLDLAHSRISDYSRRLDFRTGTYSR 138 E++ G + + MVN DW L + E+ D+ ++S Y R LD + GT +R Sbjct: 76 EKIIYGEEAPDLPEFYQTMVNLADWTGINLYLGDEKFDMLQGKVSGYKRVLDMKEGTLTR 135 Query: 139 QFIWLLGSGKKLKLTFTRLVSMTDSHLGLQQVVFEPLN 176 + W G+K+KLT R +S+ H+G+ + F P+N Sbjct: 136 ELTWESPQGRKVKLTIKRFLSLASPHIGVIKYSFTPVN 173 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.136 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,127,857,827 Number of extensions: 44429231 Number of successful extensions: 89310 Number of sequences better than 1.0e-05: 323 Number of HSP's gapped: 89054 Number of HSP's successfully gapped: 323 Length of query: 189 Length of database: 3,060,696,203 Length adjustment: 128 Effective length of query: 61 Effective length of database: 1,854,806,283 Effective search space: 113143183263 Effective search space used: 113143183263 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 127 (53.5 bits)