BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0350.0010.p (311 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C4KZB0.1 C4KZB0_EXISA Oxidoreductase domain protein 165 5e-39 B7R5Z8.1 B7R5Z8_9THEO Oxidoreductase family, NAD-binding Rossman... 161 9e-38 A9VKI0.1 A9VKI0_BACWK Oxidoreductase domain protein 160 2e-37 C2ZVI5.1 C2ZVI5_BACCE Oxidoreductase, Gfo/Idh/MocA 158 6e-37 C2ZDY3.1 C2ZDY3_BACCE Oxidoreductase, Gfo/Idh/MocA 158 6e-37 >C4KZB0.1 C4KZB0_EXISA Oxidoreductase domain protein Length = 301 Score = 165 bits (417), Expect = 5e-39, Method: Compositional matrix adjust. Identities = 112/309 (36%), Positives = 161/309 (52%), Gaps = 17/309 (5%) Query: 6 RTKVAIVGLGDIARKVYLPLLSRHDQVEVVGVLSHSPSTVQGVVHSYRFPRGTTDLKELL 65 + K+ IVGLG+IA+K YLP+LS H ++EVVG S + S + +YR T L EL Sbjct: 3 KPKIGIVGLGNIAQKAYLPILSIHPRIEVVGAFSPNTSKRDMICQTYRMTSFDT-LNELA 61 Query: 66 SWGPDAVFVHSPTPTHYDIVMKCLNHGIPVYVDKPLSYDLEESRRMAGFAEEKGLLLGVG 125 S DA FVHS T THYDIV+ L HG+ VYVDKPL+ +EE+ ++ AE G L VG Sbjct: 62 S-SIDAAFVHSSTETHYDIVLYLLEHGVDVYVDKPLAATIEEAEQLVIQAERLGRKLMVG 120 Query: 126 FNRRFAPMYTAAKAWLDTTGGISACQAFKHRTR-LQSGSSRETVXXXXXXXXXXXXWLCG 184 FNRRFAPMY L KHR + + T+ +L G Sbjct: 121 FNRRFAPMYMR----LQNESSAHMIDVVKHRENDVGPKDATFTLLDDYLHVIDTLRFLAG 176 Query: 185 EDAELLHSSLKADDAGRMLQSSGMLGWSQGATGMY--SMVRDAGADLEKLELHGNGRSAE 242 L+ + ++ + + + W Q GM+ SM R +G + E + + +G Sbjct: 177 NKLTLVSGHVDVNEKHELRFARHV--W-QDEHGMHTTSMHRTSGVNHEHVSIVRDGERLS 233 Query: 243 VADMEQATLYETGSLPRIQHFGSWDTVLERRGFSGAVNHFLNSI--DTPLQCGISASAVL 300 V +M ++ G ++ FGSWDT L +RGF GA++HF++++ DTP+Q A L Sbjct: 234 VEEMVTLHRFKQGR-HEVETFGSWDTTLVQRGFQGAIDHFISALIEDTPIQT--DGRAAL 290 Query: 301 SSHELAAKL 309 + L +KL Sbjct: 291 ETQRLVSKL 299 >B7R5Z8.1 B7R5Z8_9THEO Oxidoreductase family, NAD-binding Rossmann fold protein Length = 307 Score = 161 bits (407), Expect = 9e-38, Method: Compositional matrix adjust. Identities = 96/306 (31%), Positives = 157/306 (51%), Gaps = 8/306 (2%) Query: 6 RTKVAIVGLGDIARKVYLPLLSRHDQVEVVGVLSHSPSTVQGVVHSYRFPRGTTDLKELL 65 + K I+G G+I K YLP + +++E+VG++ + ++ Y TDL+E + Sbjct: 3 KLKTLIIGPGNIFNKAYLPFIFTLEELEIVGIVGRNEEKLKKYKEKYG-CNVYTDLEEAI 61 Query: 66 SWGPDAVFVHSPTPTHYDIVMKCLNHGIPVYVDKPLSYDLEESRRMAGFAEEKGLLLGVG 125 PD FVH+ T +HY+IV L +G+ VYVDKP++ ++++++ + A +K L+ +G Sbjct: 62 KLKPDCAFVHTATVSHYEIVRMLLENGVNVYVDKPITNEIDKTKELIDLARKKSLIFTIG 121 Query: 126 FNRRFAPMYTAAKAWLDTTGGISACQAFKHRTRLQSGSSRETVXXXXXXXXXXXXWLCGE 185 FNRR+AP+Y A + ++ C KHR + T+ ++ E Sbjct: 122 FNRRYAPLYQKAFEFFESVKP-ELCLMEKHRDNDIRDDLKYTLYDDFIHIADTLYYIVKE 180 Query: 186 DAELLHSSLKADDAGRMLQSSGMLGWSQGATGMYSMVRDAGADLEKLELHGNGRSAEVAD 245 EL LK ++A L+S + S+ +T + M R+ G D EKLE+HGN RSA V D Sbjct: 181 VGELDIHVLKENNA---LKSIMVTLKSKNSTAIGLMHRNTGEDYEKLEIHGNNRSAVVED 237 Query: 246 MEQATLYETGSLPRIQHFGSWDTVLERRGFSGAVNHFLNSI--DTPLQCGISASAVLSSH 303 ME E G + I FGSWD+ ++GF V F+ ++ D L +H Sbjct: 238 MELLRTMEKGRV-EIHTFGSWDSTSYKKGFVNIVKSFIRNVLDDNKKLANEELDYALKAH 296 Query: 304 ELAAKL 309 E+ ++ Sbjct: 297 EIVEEI 302 >A9VKI0.1 A9VKI0_BACWK Oxidoreductase domain protein Length = 308 Score = 160 bits (404), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 94/283 (33%), Positives = 147/283 (51%), Gaps = 7/283 (2%) Query: 5 GRTKVAIVGLGDIARKVYLPLLSRHDQVEVVGVLSHSPSTVQGVVHSYRFPRGTTDLKEL 64 + K+ ++GLG IA+K YLP L++ VG + +P + + YR + E Sbjct: 2 NKPKIGMIGLGSIAQKAYLPTLTKETDWNFVGAFTPNPEKRKQICQQYRIQDFHS--MET 59 Query: 65 LSWGPDAVFVHSPTPTHYDIVMKCLNHGIPVYVDKPLSYDLEESRRMAGFAEEKGLLLGV 124 L+ DA+FVHS T THY+IV + L GI VYVDKPL+ +E++ ++ +E+ L V Sbjct: 60 LASECDAIFVHSSTATHYEIVSELLKKGIDVYVDKPLAATVEQAEKLVELSEKYNRKLMV 119 Query: 125 GFNRRFAPMYTAAKAWLDTTGGISACQAFKHRT-RLQSGSSRETVXXXXXXXXXXXXWLC 183 GFNRRF PMY AAK + GIS + KHRT ++ + T+ WL Sbjct: 120 GFNRRFVPMYVAAK---EQANGISWIRIEKHRTNKVGPYTYDFTMLDDYLHIVDTARWLA 176 Query: 184 GEDAELLHSSLKADDAGRMLQSSGMLGWSQGATGMYSMVRDAGADLEKLELHGNGRSAEV 243 +D +H+ ++ ++ +L G +M R AG +LE++EL G+ V Sbjct: 177 NDDLNAVHNMMQINEKNELLYGHHTYTTPSGLLLSTAMHRHAGTNLEQIELVTTGKIIRV 236 Query: 244 ADMEQATLYETGSLPRIQHFGSWDTVLERRGFSGAVNHFLNSI 286 +M + + S+ + SWDT L++RGF AV+HF+ + Sbjct: 237 KNMNTFEIEQENSVSQ-SGSPSWDTTLKQRGFEDAVHHFIECV 278 >C2ZVI5.1 C2ZVI5_BACCE Oxidoreductase, Gfo/Idh/MocA Length = 312 Score = 158 bits (400), Expect = 6e-37, Method: Compositional matrix adjust. Identities = 97/308 (31%), Positives = 156/308 (50%), Gaps = 7/308 (2%) Query: 5 GRTKVAIVGLGDIARKVYLPLLSRHDQVEVVGVLSHSPSTVQGVVHSYRFPRGTTDLKEL 64 + K+ ++GLG IA+K YLP L++ + VG + + + V YR + E Sbjct: 6 NKPKIGMIGLGSIAQKAYLPTLTKETDWDFVGAFTPNAEKRKQVCQQYRIQDFHS--IET 63 Query: 65 LSWGPDAVFVHSPTPTHYDIVMKCLNHGIPVYVDKPLSYDLEESRRMAGFAEEKGLLLGV 124 L+ DA+FVHS T THY+IV + L GI VYVDKPL+ +E++ ++ +E+ L V Sbjct: 64 LASECDAIFVHSSTATHYEIVSELLKKGIDVYVDKPLAATIEQAEKLVELSEKYNRKLMV 123 Query: 125 GFNRRFAPMYTAAKAWLDTTGGISACQAFKHRT-RLQSGSSRETVXXXXXXXXXXXXWLC 183 GFNRRF PMY AAK + IS + KHRT ++ + T+ WL Sbjct: 124 GFNRRFVPMYVAAK---EQAHDISWIRIEKHRTNKVGPYTYDFTMLDDYLHIVDTARWLA 180 Query: 184 GEDAELLHSSLKADDAGRMLQSSGMLGWSQGATGMYSMVRDAGADLEKLELHGNGRSAEV 243 +D ++H+ ++ ++ +L S G +M R AG +LE++EL G+ V Sbjct: 181 NDDLNVVHNMMQINEKNELLYGHHTYTTSNGLLLSTAMHRHAGTNLEQIELVTTGKIIRV 240 Query: 244 ADMEQATLYETGSLPRIQHFGSWDTVLERRGFSGAVNHFLNSIDTPLQCGISASAVLSSH 303 +M + + S+ + SWDT L++RGF AV+HF+ + + + L + Sbjct: 241 KNMNTFEIEQENSVSQ-SGSPSWDTTLKQRGFEDAVHHFIECVHGNTKPVVDGLEALKTQ 299 Query: 304 ELAAKLAD 311 +L L + Sbjct: 300 QLLQSLLE 307 >C2ZDY3.1 C2ZDY3_BACCE Oxidoreductase, Gfo/Idh/MocA Length = 312 Score = 158 bits (400), Expect = 6e-37, Method: Compositional matrix adjust. Identities = 97/308 (31%), Positives = 156/308 (50%), Gaps = 7/308 (2%) Query: 5 GRTKVAIVGLGDIARKVYLPLLSRHDQVEVVGVLSHSPSTVQGVVHSYRFPRGTTDLKEL 64 + K+ ++GLG IA+K YLP L++ + VG + + + V YR + E Sbjct: 6 NKPKIGMIGLGSIAQKAYLPTLTKETDWDFVGAFTPNAEKRKQVCQQYRIQDFHS--IET 63 Query: 65 LSWGPDAVFVHSPTPTHYDIVMKCLNHGIPVYVDKPLSYDLEESRRMAGFAEEKGLLLGV 124 L+ DA+FVHS T THY+IV + L GI VYVDKPL+ +E++ ++ +E+ L V Sbjct: 64 LASECDAIFVHSSTATHYEIVSELLKKGIDVYVDKPLAATIEQAEKLVELSEKYNRKLMV 123 Query: 125 GFNRRFAPMYTAAKAWLDTTGGISACQAFKHRT-RLQSGSSRETVXXXXXXXXXXXXWLC 183 GFNRRF PMY AAK + IS + KHRT ++ + T+ WL Sbjct: 124 GFNRRFVPMYVAAK---EQAHDISWIRIEKHRTNKVGPYTYDFTMLDDYLHIVDTARWLA 180 Query: 184 GEDAELLHSSLKADDAGRMLQSSGMLGWSQGATGMYSMVRDAGADLEKLELHGNGRSAEV 243 +D ++H+ ++ ++ +L S G +M R AG +LE++EL G+ V Sbjct: 181 NDDLNVVHNMMQINEKNELLYGHHTYTTSNGLLLSTAMHRHAGTNLEQIELVTTGKIIRV 240 Query: 244 ADMEQATLYETGSLPRIQHFGSWDTVLERRGFSGAVNHFLNSIDTPLQCGISASAVLSSH 303 +M + + S+ + SWDT L++RGF AV+HF+ + + + L + Sbjct: 241 KNMNTFEIEQENSVSQ-SGSPSWDTTLKQRGFEDAVHHFIECVHGNTKPVVDGLEALKTQ 299 Query: 304 ELAAKLAD 311 +L L + Sbjct: 300 QLLQSLLE 307 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,794,257,367 Number of extensions: 73772183 Number of successful extensions: 169844 Number of sequences better than 1.0e-05: 1167 Number of HSP's gapped: 169927 Number of HSP's successfully gapped: 1168 Length of query: 311 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 176 Effective length of database: 1,788,859,178 Effective search space: 314839215328 Effective search space used: 314839215328 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)