BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0350.0009.p (204 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KJC8.1 B3KJC8_9BACI SNARE associated Golgi protein 168 2e-40 B3K9H2.1 B3K9H2_9BACI SNARE associated Golgi protein 104 5e-21 A8VZT7.1 A8VZT7_9BACI Galactokinase 100 6e-20 Q9KAJ4.1 Q9KAJ4_BACHD BH2293 protein 91 7e-17 Q65JC4.1 Q65JC4_BACLD YqeD 82 3e-14 >B3KJC8.1 B3KJC8_9BACI SNARE associated Golgi protein Length = 203 Score = 168 bits (426), Expect = 2e-40, Method: Compositional matrix adjust. Identities = 83/174 (47%), Positives = 118/174 (67%) Query: 1 MRKWLTVLLYVSGIFLAFIYRYDILAWLRQDHNXXXXXXXXXXXXXFPVVPYKLIIGIYG 60 M++W+ +LY + + LAF YR+DI WL D FP+ PYK++I I G Sbjct: 1 MKRWIIPILYAAVLALAFSYRHDITEWLESDPPLFLMILLAALLALFPIAPYKVVIAILG 60 Query: 61 YAYGSLTAALICWSASTLAGAVIYAVVKYMFKEKAGKYLTSVSALGKFTSAVQRRPFAAV 120 YAYG++ AA I W +T+A ++YA V+ +++E + L +A+ +FT+AV+RRPF ++ Sbjct: 61 YAYGTVWAAAISWFGTTIAAVILYAAVRSLYQESGRRLLGKYTAIHRFTTAVERRPFQSI 120 Query: 121 VLARLVPVIPQMGVNIYAGAAGLPFWSYLAASGLGKIPGIALYAFLGGQLFDHP 174 +LARL+PVIPQM VN+YAG A +PFW+Y AAS +GKIP I LYA+LG L DHP Sbjct: 121 LLARLLPVIPQMAVNLYAGVASIPFWTYTAASAIGKIPAILLYAWLGSGLADHP 174 >B3K9H2.1 B3K9H2_9BACI SNARE associated Golgi protein Length = 212 Score = 104 bits (259), Expect = 5e-21, Method: Compositional matrix adjust. Identities = 49/153 (32%), Positives = 84/153 (54%) Query: 24 ILAWLRQDHNXXXXXXXXXXXXXFPVVPYKLIIGIYGYAYGSLTAALICWSASTLAGAVI 83 ILAW+R + FPV+PY ++ G+ G A G + ++ W ST A ++ Sbjct: 25 ILAWIRSTDSIMLIMLVAVAMALFPVIPYPVVGGVIGAALGPVIGGMVTWVGSTAASILM 84 Query: 84 YAVVKYMFKEKAGKYLTSVSALGKFTSAVQRRPFAAVVLARLVPVIPQMGVNIYAGAAGL 143 + ++Y ++E + L ++LGK T+ +R F ++ RL+P IP + +N+YA + + Sbjct: 85 FMFIRYGYQEWGNRALHRYNSLGKVTTMFERNAFLTILFTRLIPFIPSIVINVYAALSKV 144 Query: 144 PFWSYLAASGLGKIPGIALYAFLGGQLFDHPRN 176 F SY AS LGKIP + L++ +G L +P + Sbjct: 145 TFVSYSLASALGKIPAMLLFSMVGDNLMSNPSH 177 >A8VZT7.1 A8VZT7_9BACI Galactokinase Length = 212 Score = 100 bits (250), Expect = 6e-20, Method: Compositional matrix adjust. Identities = 59/167 (35%), Positives = 90/167 (53%), Gaps = 5/167 (2%) Query: 14 IFLAFIYRYDILAWLRQDHN---XXXXXXXXXXXXXFPVVPYKLIIGIYGYAYGSLTAAL 70 I + +Y LAW+ Q+H+ FPV+PY L+ G+ G A+G LT A Sbjct: 15 ILILVLYHEAFLAWV-QNHDGDYVVGTMIIATFMSLFPVIPYPLVGGVIGAAFGPLTGAW 73 Query: 71 ICWSASTLAGAVIYAVVKYM-FKEKAGKYLTSVSALGKFTSAVQRRPFAAVVLARLVPVI 129 I W ST+A A+ + +V+Y F E K L + K T +R F ++ + R++PVI Sbjct: 74 IIWFGSTMASAIFFLLVRYGGFHEMGTKILLKYNVTKKITLLFERNAFLSITILRMIPVI 133 Query: 130 PQMGVNIYAGAAGLPFWSYLAASGLGKIPGIALYAFLGGQLFDHPRN 176 P + +N YA + + F Y ASGLGKIP +AL+A +G + +P + Sbjct: 134 PSIVINAYAALSRIRFPVYAVASGLGKIPAMALFALVGHTIVTNPID 180 >Q9KAJ4.1 Q9KAJ4_BACHD BH2293 protein Length = 212 Score = 90.5 bits (223), Expect = 7e-17, Method: Compositional matrix adjust. Identities = 49/168 (29%), Positives = 87/168 (51%), Gaps = 4/168 (2%) Query: 13 GIFLAFIYRYD--ILAWLRQD--HNXXXXXXXXXXXXXFPVVPYKLIIGIYGYAYGSLTA 68 G+ A IY Y +L W++Q FP++PY +I G+ G AYG Sbjct: 12 GVIAALIYLYGEWLLEWIQQGGARFIFLTVIIATLLALFPIIPYPIIGGVLGAAYGPFLG 71 Query: 69 ALICWSASTLAGAVIYAVVKYMFKEKAGKYLTSVSALGKFTSAVQRRPFAAVVLARLVPV 128 + + W S+LA +++ V+Y +++ + + K T+ ++ F A+ + RL+P+ Sbjct: 72 SAVTWIGSSLASIIMFMFVRYGYQDWGLSLIRRSDLVTKVTAMFEKNAFMAIFITRLIPI 131 Query: 129 IPQMGVNIYAGAAGLPFWSYLAASGLGKIPGIALYAFLGGQLFDHPRN 176 IP + VNIY+ + + F +Y AS LGK+P + L+A +G + +P Sbjct: 132 IPSIIVNIYSALSRVSFINYSIASSLGKVPSMILFAVVGNSIATNPSQ 179 >Q65JC4.1 Q65JC4_BACLD YqeD Length = 216 Score = 82.0 bits (201), Expect = 3e-14, Method: Compositional matrix adjust. Identities = 47/175 (26%), Positives = 90/175 (51%), Gaps = 2/175 (1%) Query: 1 MRKWLTVLLYVSGIFLAFIYRYDILAWLRQD--HNXXXXXXXXXXXXXFPVVPYKLIIGI 58 +RK T+++ S I + F+ + L +R ++ FP+VP+ LI G+ Sbjct: 2 IRKLFTIIIMASIIAVGFVCKDSWLDVIRDGGVYSVLFSMLLVAACVFFPIVPFALIAGL 61 Query: 59 YGYAYGSLTAALICWSASTLAGAVIYAVVKYMFKEKAGKYLTSVSALGKFTSAVQRRPFA 118 G +G LI + S L +++ + +Y F++ A ++LT + ++ + + F Sbjct: 62 NGALFGIANGVLITLTGSMLGTMLLFFLSRYGFRDMARRHLTKYPKMSEYEAYFNQNAFT 121 Query: 119 AVVLARLVPVIPQMGVNIYAGAAGLPFWSYLAASGLGKIPGIALYAFLGGQLFDH 173 AV+L RL+PVIP + +NI G + + + + AAS LGK+P + + + G ++ Sbjct: 122 AVLLGRLIPVIPAVVMNIVCGLSKVKWAVFFAASTLGKVPNVLVVSIAGANFSEN 176 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.327 0.142 0.451 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,043,035,211 Number of extensions: 36779728 Number of successful extensions: 124565 Number of sequences better than 1.0e-05: 193 Number of HSP's gapped: 124794 Number of HSP's successfully gapped: 193 Length of query: 204 Length of database: 3,060,696,203 Length adjustment: 129 Effective length of query: 75 Effective length of database: 1,845,385,268 Effective search space: 138403895100 Effective search space used: 138403895100 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 128 (53.9 bits)