BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0350.0003.p (158 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K5X3.1 B3K5X3_9BACI 5-methyltetrahydropteroyltriglutamate--hom... 239 7e-62 B1DKN8.1 B1DKN8_9BACL 5-methyltetrahydropteroyltriglutamate--hom... 232 7e-60 A9U742.1 A9U742_PHYPA Predicted protein 231 1e-59 Q9KFP1.1 METE_BACHD 5-methyltetrahydropteroyltriglutamate--homoc... 227 2e-58 C0ZEA1.1 C0ZEA1_BREBN 5-methyltetrahydropteroyltriglutamate--hom... 224 1e-57 >B3K5X3.1 B3K5X3_9BACI 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase EC=2.1.1.14 Length = 762 Score = 239 bits (609), Expect = 7e-62, Method: Compositional matrix adjust. Identities = 114/156 (73%), Positives = 126/156 (80%) Query: 1 VDEPAIREGLPLKAEDHAHYLNWAVRSFRVATNHVKDTTQVHTHMCYSEFNDMIGAISAM 60 VDEPA+REGLPLK ED A YL WAV++FR+ T V+ TTQ+HTHMCY EF+DMI +I AM Sbjct: 602 VDEPAVREGLPLKEEDQAEYLAWAVKAFRMTTCTVQPTTQIHTHMCYCEFHDMIDSIEAM 661 Query: 61 DADVISIETSRSHGELIAAFEEDDYDKGIGLGVYDIHSPRVPEVEEMTHNIERALRVLDA 120 DADVISIETSRSHGELI +FE + Y GIGLGVYDIHSPRVP VEEMT IERALRVLD Sbjct: 662 DADVISIETSRSHGELIHSFEVNTYKLGIGLGVYDIHSPRVPRVEEMTSMIERALRVLDP 721 Query: 121 GQFWINPDCGLKTRGWEETEAALRNMVLAAANVRKN 156 FWINPDCGLKTRG EET A+LRNMV A R + Sbjct: 722 KLFWINPDCGLKTRGMEETVASLRNMVQATEIARSS 757 >B1DKN8.1 B1DKN8_9BACL 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase EC=2.1.1.14 Length = 762 Score = 232 bits (592), Expect = 7e-60, Method: Compositional matrix adjust. Identities = 109/154 (70%), Positives = 127/154 (82%) Query: 1 VDEPAIREGLPLKAEDHAHYLNWAVRSFRVATNHVKDTTQVHTHMCYSEFNDMIGAISAM 60 VDEPA+REGLPLK E+ YL+WAV++FR+ T V+DTTQ+HTHMCY EF+DMI +I M Sbjct: 602 VDEPAVREGLPLKEEEQKPYLDWAVKAFRMTTCTVQDTTQIHTHMCYCEFHDMIDSIEEM 661 Query: 61 DADVISIETSRSHGELIAAFEEDDYDKGIGLGVYDIHSPRVPEVEEMTHNIERALRVLDA 120 DADVISIETSRSHGELI +FE + Y GIGLGVYDIHSPRVP+V+EMT+ I+RAL+VLD Sbjct: 662 DADVISIETSRSHGELIHSFEVNTYKHGIGLGVYDIHSPRVPDVQEMTNMIDRALQVLDP 721 Query: 121 GQFWINPDCGLKTRGWEETEAALRNMVLAAANVR 154 FWINPDCGLKTRG EET A+LRNMV A R Sbjct: 722 KLFWINPDCGLKTRGKEETIASLRNMVEATKLAR 755 >A9U742.1 A9U742_PHYPA Predicted protein Length = 269 Score = 231 bits (589), Expect = 1e-59, Method: Compositional matrix adjust. Identities = 110/158 (69%), Positives = 127/158 (80%) Query: 1 VDEPAIREGLPLKAEDHAHYLNWAVRSFRVATNHVKDTTQVHTHMCYSEFNDMIGAISAM 60 VDEPA+REGLPLK +D YL WAV++FR+ T V+++TQ+HTHMCY EF+DMI +I M Sbjct: 109 VDEPAVREGLPLKEQDQKGYLEWAVKAFRLTTCTVQNSTQIHTHMCYCEFHDMIDSIIDM 168 Query: 61 DADVISIETSRSHGELIAAFEEDDYDKGIGLGVYDIHSPRVPEVEEMTHNIERALRVLDA 120 DADVISIETSRSHGELI +FEE+ Y GIGLGVYDIHSPRVP+VEEMT I+RALRVLD Sbjct: 169 DADVISIETSRSHGELIQSFEENTYPLGIGLGVYDIHSPRVPDVEEMTRMIDRALRVLDP 228 Query: 121 GQFWINPDCGLKTRGWEETEAALRNMVLAAANVRKNNA 158 FWINPDCGLKTRG ET A+LRNMV A R+ A Sbjct: 229 KLFWINPDCGLKTRGIGETVASLRNMVEATRLAREKYA 266 >Q9KFP1.1 METE_BACHD 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase EC=2.1.1.14 Length = 756 Score = 227 bits (579), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 106/147 (72%), Positives = 122/147 (82%) Query: 1 VDEPAIREGLPLKAEDHAHYLNWAVRSFRVATNHVKDTTQVHTHMCYSEFNDMIGAISAM 60 +DEPAIREGLPLKAED YL+WAV +FR + HVK TTQ+HTHMCYSEF++MI AI + Sbjct: 596 IDEPAIREGLPLKAEDQQEYLDWAVSAFRASCAHVKATTQIHTHMCYSEFHEMIEAIDDL 655 Query: 61 DADVISIETSRSHGELIAAFEEDDYDKGIGLGVYDIHSPRVPEVEEMTHNIERALRVLDA 120 DADVISIETSRSHGE+I+AFE+ Y+KGIGLGVYDIHSPRVP EEM + I RAL VL A Sbjct: 656 DADVISIETSRSHGEMISAFEKTTYEKGIGLGVYDIHSPRVPSEEEMLNVIRRALTVLPA 715 Query: 121 GQFWINPDCGLKTRGWEETEAALRNMV 147 FW+NPDCGLKTR +ET AAL+NMV Sbjct: 716 SLFWVNPDCGLKTRAEKETVAALKNMV 742 >C0ZEA1.1 C0ZEA1_BREBN 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase EC=2.1.1.14 Length = 763 Score = 224 bits (572), Expect = 1e-57, Method: Compositional matrix adjust. Identities = 103/154 (66%), Positives = 121/154 (78%) Query: 1 VDEPAIREGLPLKAEDHAHYLNWAVRSFRVATNHVKDTTQVHTHMCYSEFNDMIGAISAM 60 VDEPA+REGLPLK E+ A YL WAVR+FR+AT+ V +TQ+HTHMCY EF+D + IS + Sbjct: 601 VDEPALREGLPLKKEEQAAYLEWAVRAFRLATSTVAPSTQIHTHMCYCEFHDFMDVISEL 660 Query: 61 DADVISIETSRSHGELIAAFEEDDYDKGIGLGVYDIHSPRVPEVEEMTHNIERALRVLDA 120 DADVISIETSRSHGEL++ FE YDKGIGLGVYDIHSPRVP V EM + R +++L Sbjct: 661 DADVISIETSRSHGELVSVFETGTYDKGIGLGVYDIHSPRVPAVSEMEDMVARGVQLLPP 720 Query: 121 GQFWINPDCGLKTRGWEETEAALRNMVLAAANVR 154 QFWINPDCGLKTRG EET +LRNMV A +R Sbjct: 721 DQFWINPDCGLKTRGREETVQSLRNMVAATKAIR 754 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.132 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 936,672,748 Number of extensions: 35769932 Number of successful extensions: 79838 Number of sequences better than 1.0e-05: 935 Number of HSP's gapped: 79562 Number of HSP's successfully gapped: 935 Length of query: 158 Length of database: 3,060,696,203 Length adjustment: 119 Effective length of query: 39 Effective length of database: 1,939,595,418 Effective search space: 75644221302 Effective search space used: 75644221302 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 125 (52.8 bits)