BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0349.0013.p (166 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K6Y9.1 B3K6Y9_9BACI Pseudouridine synthase 216 4e-55 A3DC38.1 A3DC38_CLOTH Ribosomal large subunit pseudouridine synt... 194 2e-48 B4BE29.1 B4BE29_CLOTM Pseudouridine synthase 194 2e-48 A8MM82.1 A8MM82_ALKOO Pseudouridine synthase 182 7e-45 B0A6K4.1 B0A6K4_9CLOT Putative uncharacterized protein 176 5e-43 >B3K6Y9.1 B3K6Y9_9BACI Pseudouridine synthase Length = 239 Score = 216 bits (551), Expect = 4e-55, Method: Compositional matrix adjust. Identities = 106/157 (67%), Positives = 125/157 (79%) Query: 1 MIFAKTSKAASRLSESVRSHSFHKVYLTVVHGKPPAAQGRLVHTLLKDAKTNTVTIVRKG 60 M+FAKTSKAASRLSE+VRS SF K Y+ VVHG P GRLV+ L KDAKTNTV + KG Sbjct: 76 MVFAKTSKAASRLSEAVRSRSFRKQYVAVVHGVPAKTSGRLVNMLYKDAKTNTVHVTSKG 135 Query: 61 TPGGKEAILDYTVIGSAEGYSLLKIDLLTGRSHQIRVQLSGIRCPLYGDQKYGAAVNKPG 120 T G KEAILDYTV+ +AEG SL+ I+L TGR HQIRVQLS PL+GDQKYG+ VNK G Sbjct: 136 TDGAKEAILDYTVLQAAEGVSLVLIELHTGRPHQIRVQLSHAGHPLFGDQKYGSKVNKAG 195 Query: 121 QQIALWSAVVGFPHPVTKEEIELISLPPQTHPWSLWS 157 QQIALWS +VGFPHPVTKE+I + S+PP +PWS+++ Sbjct: 196 QQIALWSVLVGFPHPVTKEDISIRSMPPSVYPWSIFN 232 >A3DC38.1 A3DC38_CLOTH Ribosomal large subunit pseudouridine synthase D EC=5.4.99.- Length = 231 Score = 194 bits (493), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 93/158 (58%), Positives = 117/158 (74%) Query: 1 MIFAKTSKAASRLSESVRSHSFHKVYLTVVHGKPPAAQGRLVHTLLKDAKTNTVTIVRKG 60 M+FAKTSKAASRLS+ VR+ F KVYL VVHG +GRL + LLKD + NTV++V++ Sbjct: 73 MVFAKTSKAASRLSDQVRTRQFKKVYLAVVHGILDKLEGRLENYLLKDTRNNTVSVVKED 132 Query: 61 TPGGKEAILDYTVIGSAEGYSLLKIDLLTGRSHQIRVQLSGIRCPLYGDQKYGAAVNKPG 120 TPGGK A+LDY V G G+SL+K+ L TGR HQIRVQL+ I PLYGDQKYG VNKPG Sbjct: 133 TPGGKLAVLDYKVEGIYNGFSLVKVSLHTGRPHQIRVQLANIGHPLYGDQKYGHGVNKPG 192 Query: 121 QQIALWSAVVGFPHPVTKEEIELISLPPQTHPWSLWSQ 158 QQIALWS+ + F HPV K+E+ PP+ PW+ +++ Sbjct: 193 QQIALWSSEIAFVHPVLKQEMIFSCEPPEVEPWTWFNR 230 >B4BE29.1 B4BE29_CLOTM Pseudouridine synthase Length = 231 Score = 194 bits (493), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 93/158 (58%), Positives = 117/158 (74%) Query: 1 MIFAKTSKAASRLSESVRSHSFHKVYLTVVHGKPPAAQGRLVHTLLKDAKTNTVTIVRKG 60 M+FAKTSKAASRLS+ VR+ F KVYL VVHG +GRL + LLKD + NTV++V++ Sbjct: 73 MVFAKTSKAASRLSDQVRTRQFKKVYLAVVHGILDKLEGRLENYLLKDTRNNTVSVVKED 132 Query: 61 TPGGKEAILDYTVIGSAEGYSLLKIDLLTGRSHQIRVQLSGIRCPLYGDQKYGAAVNKPG 120 TPGGK A+LDY V G G+SL+K+ L TGR HQIRVQL+ I PLYGDQKYG VNKPG Sbjct: 133 TPGGKLAVLDYKVEGIYNGFSLVKVSLHTGRPHQIRVQLANIGHPLYGDQKYGHGVNKPG 192 Query: 121 QQIALWSAVVGFPHPVTKEEIELISLPPQTHPWSLWSQ 158 QQIALWS+ + F HPV K+E+ PP+ PW+ +++ Sbjct: 193 QQIALWSSEIAFVHPVLKQEMIFSCEPPEVEPWTWFNR 230 >A8MM82.1 A8MM82_ALKOO Pseudouridine synthase Length = 230 Score = 182 bits (463), Expect = 7e-45, Method: Compositional matrix adjust. Identities = 83/156 (53%), Positives = 112/156 (71%) Query: 1 MIFAKTSKAASRLSESVRSHSFHKVYLTVVHGKPPAAQGRLVHTLLKDAKTNTVTIVRKG 60 M+FAKTSK+A+RLS+ +R F K Y+ VVHG+P + LVH LLKD K+N V++V+K Sbjct: 69 MVFAKTSKSAARLSDQIRKREFKKTYMAVVHGRPMKEKETLVHYLLKDNKSNIVSVVKKD 128 Query: 61 TPGGKEAILDYTVIGSAEGYSLLKIDLLTGRSHQIRVQLSGIRCPLYGDQKYGAAVNKPG 120 G KEAILDY ++ + E YSL+KI+L TGR HQIRVQ + I PL+GDQ+YG VN+ G Sbjct: 129 REGAKEAILDYEIVETVESYSLVKINLHTGRPHQIRVQFATIGNPLHGDQRYGKGVNQVG 188 Query: 121 QQIALWSAVVGFPHPVTKEEIELISLPPQTHPWSLW 156 QQIALWS + HP +KE++ SLP + PW+++ Sbjct: 189 QQIALWSHEIACQHPTSKEDMVFTSLPEKRMPWNMF 224 >B0A6K4.1 B0A6K4_9CLOT Putative uncharacterized protein Length = 221 Score = 176 bits (447), Expect = 5e-43, Method: Compositional matrix adjust. Identities = 82/156 (52%), Positives = 103/156 (66%) Query: 1 MIFAKTSKAASRLSESVRSHSFHKVYLTVVHGKPPAAQGRLVHTLLKDAKTNTVTIVRKG 60 M+FAKTSKAASRLSE VR+ +F K Y V+HG+ Q L L KD KTN V++V K Sbjct: 66 MVFAKTSKAASRLSEQVRNKTFKKTYKAVLHGEMKKKQDNLSDYLYKDKKTNMVSVVNKN 125 Query: 61 TPGGKEAILDYTVIGSAEGYSLLKIDLLTGRSHQIRVQLSGIRCPLYGDQKYGAAVNKPG 120 K+A L Y + S G+SL++IDL TGR HQIRVQ S PLYGDQ+YG VNK G Sbjct: 126 HKDAKDAKLSYKTLASNNGFSLVEIDLQTGRPHQIRVQFSSRNHPLYGDQRYGKNVNKKG 185 Query: 121 QQIALWSAVVGFPHPVTKEEIELISLPPQTHPWSLW 156 QQIALWS + HP KE++E + PP +PW+++ Sbjct: 186 QQIALWSYKIEIEHPTKKEKMEFVCEPPNKYPWNMF 221 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.133 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,008,237,710 Number of extensions: 39393008 Number of successful extensions: 92180 Number of sequences better than 1.0e-05: 2539 Number of HSP's gapped: 91631 Number of HSP's successfully gapped: 2539 Length of query: 166 Length of database: 3,060,696,203 Length adjustment: 125 Effective length of query: 41 Effective length of database: 1,883,069,328 Effective search space: 77205842448 Effective search space used: 77205842448 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 126 (53.1 bits)