BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0349.0005.p (306 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K6Z7.1 B3K6Z7_9BACI Lipolytic protein G-D-S-L family 234 1e-59 B1DES2.1 B1DES2_9BACL Lipolytic protein G-D-S-L family 162 3e-38 C5D2B2.1 C5D2B2_9BACI Lipolytic protein G-D-S-L family 142 3e-32 B4BJY2.1 B4BJY2_9BACI Lipolytic protein G-D-S-L family 137 9e-31 C3IZR9.1 C3IZR9_9BACI Lipolytic protein G-D-S-L family 135 4e-30 >B3K6Z7.1 B3K6Z7_9BACI Lipolytic protein G-D-S-L family Length = 283 Score = 234 bits (596), Expect = 1e-59, Method: Compositional matrix adjust. Identities = 127/318 (39%), Positives = 176/318 (55%), Gaps = 49/318 (15%) Query: 1 LNDSKWTWRAVXXXXXXXXXXXXGGFVYAVKDILYPRG--------XXXXXXXXXXXXXX 52 + S W WR GFVYA KDI P+G Sbjct: 1 MKSSAWIWRITASVSIIVTIVLIFGFVYAFKDINDPQGQPLLTGGESAAPGQETAPPKPE 60 Query: 53 XXXXDGVI----KIVALGDSLAKGTGDNEGSGFVKRTIDGLTAKGNKANLVGNLGINGMT 108 + V+ K+ A+GDSLAKGTGD+ G GFV+R+++ L G A+L+ NL INGMT Sbjct: 61 RAPSEAVLPNDLKVTAIGDSLAKGTGDSTGGGFVRRSVEQLNGDGRTASLLANLAINGMT 120 Query: 109 TAGLQSRLQQEGVKYALRQSNVILVSIGGNDLFRGSDLL-GSSRKGQQIATPETENTXXX 167 T GL +L +G++YAL+++NVI++SIGGND+F+GS L+ G++ G+ P Sbjct: 121 TEGLLPKLDDKGIQYALKEANVIMLSIGGNDIFQGSGLMEGAADAGELELDP-------- 172 Query: 168 XXXXXXXXXXXXXXXXXXXXXXXXDXXXXXXXXXXXXXXKRLNTILERIAEINPEAQIFY 227 +RL TILE+I +INP A+I Y Sbjct: 173 ----------------------------AMLMGALPQASERLQTILEKIRDINPNARIVY 204 Query: 228 MGLYNPFGDIPELLVPGNQAVTAWNNAAMDIINKHSNMTLVPTFDLFNRHLDKYLSSDHF 287 +GLY+PF D+ ELL+PGN V+AWN+A M+++N+ NMTLVPTFDLF L +YLS+DHF Sbjct: 205 IGLYHPFSDLEELLIPGNVVVSAWNHAVMELVNRDPNMTLVPTFDLFQHKLGEYLSTDHF 264 Query: 288 HPNGDGYQRMSERIVQAV 305 HPNG GYQ +++RIV+ + Sbjct: 265 HPNGAGYQAIADRIVEGM 282 >B1DES2.1 B1DES2_9BACL Lipolytic protein G-D-S-L family Length = 275 Score = 162 bits (410), Expect = 3e-38, Method: Compositional matrix adjust. Identities = 104/304 (34%), Positives = 145/304 (47%), Gaps = 43/304 (14%) Query: 8 WRAVXXXXXXXXXXXXGGFVYAVKDILYPRGXXXXXXXXXXXXXXX-XXXDGV-IKIVAL 65 W+ + GF +AVKD+LYP+G +G I I A+ Sbjct: 10 WKIIGISAVLSTVLLAAGFGFAVKDLLYPKGEVFAGSVNVPALPAGGKLSEGKNIVITAI 69 Query: 66 GDSLAKGTGDNEGSGFVKRTIDGLTAK--GNKANLVGNLGINGMTTAGLQSRLQQE-GVK 122 GDSL KGTGD G G+VK+ + L K L NL I G+ L L+ + G Sbjct: 70 GDSLTKGTGDASGEGYVKQVVALLKQKYPDTSVRLNNNLAIGGLKANRLAQMLETDKGYS 129 Query: 123 YALRQSNVILVSIGGNDLFRGSDLLGSSRKGQQIATPETENTXXXXXXXXXXXXXXXXXX 182 +ALRQ+NVI+ +IGGNDLF + +K Sbjct: 130 FALRQANVIVFTIGGNDLFHIASGTSPGKKA----------------------------- 160 Query: 183 XXXXXXXXXDXXXXXXXXXXXXXXKRLNTILERIAEINPEAQIFYMGLYNPFGDIPELLV 242 D RL I++ + INP AQ+ Y+GLYNPF DIPE Sbjct: 161 --------GDLNLDKLKAELPQGLNRLAQIVKHLHAINPNAQVVYVGLYNPFYDIPEYR- 211 Query: 243 PGNQAVTAWNNAAMDIINKHSNMTLVPTFDLFNRHLDKYLSSDHFHPNGDGYQRMSERIV 302 G+ + WN+ A +++++ NMT+VPTFDLF + +YLSSDHFHPN DGY +++ RIV Sbjct: 212 GGSLQIQQWNDRAYALLHQYPNMTMVPTFDLFENAIGRYLSSDHFHPNHDGYAQIAARIV 271 Query: 303 QAVQ 306 Q+++ Sbjct: 272 QSME 275 >C5D2B2.1 C5D2B2_9BACI Lipolytic protein G-D-S-L family Length = 264 Score = 142 bits (359), Expect = 3e-32, Method: Compositional matrix adjust. Identities = 82/246 (33%), Positives = 122/246 (49%), Gaps = 45/246 (18%) Query: 60 IKIVALGDSLAKGTGDNEGSGFVKRTIDGLTAKGNKANLVGNLGINGMTTAGLQSRLQQE 119 I IVALGDSL +GTGD G G++ +D L K K V NL I G + GL +L Q Sbjct: 56 IYIVALGDSLTRGTGDESGKGYIGYMVDSLRKKTTKPIRVTNLAIKGQRSDGLLKQLGQA 115 Query: 120 GVKYALRQSNVILVSIGGNDLFRGSDLLGSSRKGQQIATPETENTXXXXXXXXXXXXXXX 179 +K L+ +++I+++IGGNDLF+G + L ++A + E Sbjct: 116 EIKRQLKMADIIVMTIGGNDLFQGGEAL-------KLAPKQIEQAKNAYL---------- 158 Query: 180 XXXXXXXXXXXXDXXXXXXXXXXXXXXKRLNTILERIAEINPEAQIFYMGLYNPFGDIPE 239 L+ I + I +N +A +FY+GLYNPF D+ + Sbjct: 159 ---------------------------HNLDRIFQTIRSVNKDAVVFYIGLYNPFSDLGD 191 Query: 240 LLVPGNQAVTAWNNAAMDIINKHSNMTLVPTFDLFNRHLDKYLSSDHFHPNGDGYQRMSE 299 + V WN A+ + ++ N+ VP FDLF H++ YL SDHFHPN +GY+R+ E Sbjct: 192 A-KKTSAIVRQWNFASAETAARYPNIIAVPIFDLFELHVNDYLYSDHFHPNKEGYKRIGE 250 Query: 300 RIVQAV 305 R+ + Sbjct: 251 RVASLI 256 >B4BJY2.1 B4BJY2_9BACI Lipolytic protein G-D-S-L family Length = 265 Score = 137 bits (346), Expect = 9e-31, Method: Compositional matrix adjust. Identities = 84/255 (32%), Positives = 124/255 (48%), Gaps = 57/255 (22%) Query: 57 DGVIKIVALGDSLAKGTGDNEGSGFVKRTIDGLTAKGNKANLVGNLGINGMTTAGLQSRL 116 DG I IVALGDSL +GTGD G G++ +D L + ++ V NL I G+ + GL +L Sbjct: 54 DGEIDIVALGDSLTRGTGDESGKGYIGYMVDELRQQTDEPIRVTNLAIRGLRSDGLLRQL 113 Query: 117 QQEGVKYALRQSNVILVSIGGNDLFRGSDLLG------SSRKGQQIATPETENTXXXXXX 170 Q ++ + +++I+++IGGNDLF+G + L KGQ IA Sbjct: 114 GQSEIQRQIAMADLIVMTIGGNDLFQGGEALEWNVKELDEAKGQYIAN------------ 161 Query: 171 XXXXXXXXXXXXXXXXXXXXXDXXXXXXXXXXXXXXKRLNTILERIAEINPEAQIFYMGL 230 L+ I + +N EA IF +GL Sbjct: 162 --------------------------------------LDRIFALLRRLNSEAVIFAIGL 183 Query: 231 YNPFGDIPELLVPGNQAVTAWNNAAMDIINKHSNMTLVPTFDLFNRHLDKYLSSDHFHPN 290 YNPF D+ + + V WN A+ ++ + N+ VPTFDLF H++ YL SDHFHPN Sbjct: 184 YNPFSDLDDA-KRTSAIVRDWNFASAEVAAHYPNIVAVPTFDLFALHVNDYLYSDHFHPN 242 Query: 291 GDGYQRMSERIVQAV 305 +GY+R+ ER+ + Sbjct: 243 KEGYKRIGERVASLI 257 >C3IZR9.1 C3IZR9_9BACI Lipolytic protein G-D-S-L family Length = 265 Score = 135 bits (341), Expect = 4e-30, Method: Compositional matrix adjust. Identities = 85/253 (33%), Positives = 123/253 (48%), Gaps = 53/253 (20%) Query: 57 DGVIKIVALGDSLAKGTGDNEGSGFVKRTIDGLTAKGNKANLVGNLGINGMTTAGLQSRL 116 D + IVALGDSL +GTGD G G+V +D L + +K V NL I G+ + GL +L Sbjct: 54 DEKMDIVALGDSLTRGTGDESGKGYVGYMVDELRRQTDKPIRVTNLAIRGLRSDGLLRQL 113 Query: 117 QQEGVKYALRQSNVILVSIGGNDLFRGSDLLGSSRKGQQIATPETENTXXXXXXXXXXXX 176 Q ++ + +++I+++IGGNDLF+G + L RK A Sbjct: 114 GQPEIQRQVAMADLIVMTIGGNDLFQGGEALKLDRKQLNEAK------------------ 155 Query: 177 XXXXXXXXXXXXXXXDXXXXXXXXXXXXXXKRLNTILERI----AEINPEAQIFYMGLYN 232 +R L+RI N EA IF +GLYN Sbjct: 156 ------------------------------RRYVANLDRIFAALRRFNSEAVIFAIGLYN 185 Query: 233 PFGDIPELLVPGNQAVTAWNNAAMDIINKHSNMTLVPTFDLFNRHLDKYLSSDHFHPNGD 292 PFGD+ + + V WN A+ ++ ++ N+ VPTFDLF H++ YL SDHFHPN Sbjct: 186 PFGDLDD-AKRTSAVVRDWNFASAEVAARYPNIVAVPTFDLFALHVNDYLYSDHFHPNAA 244 Query: 293 GYQRMSERIVQAV 305 GY+R+ ER+ + Sbjct: 245 GYKRIGERVASLI 257 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.136 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,441,966,826 Number of extensions: 51783482 Number of successful extensions: 127681 Number of sequences better than 1.0e-05: 250 Number of HSP's gapped: 127653 Number of HSP's successfully gapped: 354 Length of query: 306 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 171 Effective length of database: 1,788,859,178 Effective search space: 305894919438 Effective search space used: 305894919438 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)