BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0349.0001.p (280 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DI28.1 B1DI28_9BACL KWG Leptospira repeat protein 202 4e-50 Q895B3.1 Q895B3_CLOTE Conserved protein 183 1e-44 B3K663.1 B3K663_9BACI KWG Leptospira repeat protein 181 7e-44 B1KYA1.1 B1KYA1_CLOBM KWG leptospira repeat protein 164 8e-39 C3L366.1 C3L366_CLOB6 KWG leptospira repeat protein 161 5e-38 >B1DI28.1 B1DI28_9BACL KWG Leptospira repeat protein Length = 681 Score = 202 bits (513), Expect = 4e-50, Method: Compositional matrix adjust. Identities = 121/279 (43%), Positives = 150/279 (53%), Gaps = 43/279 (15%) Query: 5 ILNELIKGWDNGLVQTE--VWRWDGIGWNECIPQQEEDFIRADEDLFVTIPAVAGLYRVD 62 +N LI W G QT V+ W +G+ +P D+ L T Sbjct: 71 FVNNLIINWRTGDEQTRRAVYEWSPVGFRNIVP---------DDRLLET----------- 110 Query: 63 YSRKXXXXXXXXXXXXXSALRAELLHPAPFKLKEGTLWGYINNEGKTAIEPRYDYAEEFQ 122 RA L+P K GT WGYIN G I PR+D AE+FQ Sbjct: 111 --------------------RAISLYPGSEKTTSGTRWGYINGIGTMVIPPRFDRAEDFQ 150 Query: 123 ANGLAVVQRKDKSGLIDSTGREKVKPVYSFIAPFSEGRAVVSDAKG-YTLIDEKGKEVTP 181 NGLA+V + +GLI++TG V+PVYSFIAPFSEGRAVV D KG Y L+++ G VT Sbjct: 151 TNGLAIVSADNGNGLINTTGAYFVRPVYSFIAPFSEGRAVVIDDKGKYRLLNDSGIIVTS 210 Query: 182 ARADYLNSLQEGRALFSKQSTTGKSLYGYWDAQGKEVLPAVYEDAGDFAAGSALVKIKDG 241 Y+ + +GRA+ + Q GK++YGY D GKEV+P YEDA DF+ A+VKIKD Sbjct: 211 NGYSYMADMHDGRAMVNNQLEGGKTVYGYIDRSGKEVIPLRYEDATDFSNRHAVVKIKDN 270 Query: 242 EYALIDPQGAVLHTYHHPFVGYPGDGLLAFQAEENGKYG 280 YALI G+ YH PFVG GDGLL FQ NGKYG Sbjct: 271 VYALIRHDGSKEAEYHFPFVGVLGDGLLPFQQTANGKYG 309 Score = 63.2 bits (152), Expect = 2e-08, Method: Compositional matrix adjust. Identities = 62/224 (27%), Positives = 96/224 (42%), Gaps = 59/224 (26%) Query: 98 TLWGYINNEGKTAIEPRYDYAEEFQANGLAVVQRKD------------------------ 133 T++GYI+ GK I RY+ A +F +N AVV+ KD Sbjct: 235 TVYGYIDRSGKEVIPLRYEDATDF-SNRHAVVKIKDNVYALIRHDGSKEAEYHFPFVGVL 293 Query: 134 -------------KSGLIDSTGREKVKPVYSFIAPFSEGRAVVSDAKG----YTLIDEKG 176 K G I+ G K++P YS PF GRA+V++A+ Y +ID KG Sbjct: 294 GDGLLPFQQTANGKYGYINEKGTVKIQPTYSMAFPFQNGRAIVNNAEDFGAEYGVIDTKG 353 Query: 177 KEVTPARADYLNSLQE-----GRALFSKQSTTGKSLYGYWDAQGKEVLPAVYEDAGDFAA 231 V + + ++ + G+A+ QS G S+Y +D+ GK + VY D + Sbjct: 354 NLVIQTIYNDIMAIGQQRYAVGKAIDPDQSFLG-SMYAIFDSDGKRLTDFVYTDVQPYRK 412 Query: 232 GSALVKIKDGEYALIDPQGAVLHTYHHPFVGYP---GDGLLAFQ 272 G L + +G+ ++T +P GYP G G L + Sbjct: 413 G--LASVSNGKTTFF------INTSGNPAPGYPQFQGSGTLKLE 448 >Q895B3.1 Q895B3_CLOTE Conserved protein Length = 845 Score = 183 bits (465), Expect = 1e-44, Method: Compositional matrix adjust. Identities = 88/194 (45%), Positives = 128/194 (65%) Query: 87 LHPAPFKLKEGTLWGYINNEGKTAIEPRYDYAEEFQANGLAVVQRKDKSGLIDSTGREKV 146 L+PA K +G LWGYINN G+ I+P+Y A +FQ NGLA+V+ + G+I+ +G+ V Sbjct: 281 LYPASIKTAKGVLWGYINNRGELIIKPQYGQAFDFQNNGLAIVELNNLYGIINQSGKYVV 340 Query: 147 KPVYSFIAPFSEGRAVVSDAKGYTLIDEKGKEVTPARADYLNSLQEGRALFSKQSTTGKS 206 +P Y I+ FSEGRA V D++G+ +IDEKG+EVT DY+ + ++GRA+F G Sbjct: 341 EPKYESISEFSEGRATVIDSRGFKVIDEKGREVTSKAYDYIGTYKDGRAVFGNSGDNGAY 400 Query: 207 LYGYWDAQGKEVLPAVYEDAGDFAAGSALVKIKDGEYALIDPQGAVLHTYHHPFVGYPGD 266 YGY D + +E++PA Y+ AGDF A+VK+K+ E+ LID G L+ Y + VG + Sbjct: 401 FYGYLDKEEREIIPARYQSAGDFKDEKAIVKVKENEFRLIDRNGQTLNIYKYNLVGNLSE 460 Query: 267 GLLAFQAEENGKYG 280 GLLAFQ + + K+G Sbjct: 461 GLLAFQEKPDSKFG 474 Score = 58.5 bits (140), Expect = 6e-07, Method: Compositional matrix adjust. Identities = 60/194 (30%), Positives = 87/194 (44%), Gaps = 15/194 (7%) Query: 90 APFKLKEGTLWGYINNEGKTAIEPRYDYAEEFQANGLAVVQRKD-KSGLIDSTGREKVKP 148 A K+KE + I+ G+T +Y+ LA ++ D K G I+ G +KP Sbjct: 428 AIVKVKENE-FRLIDRNGQTLNIYKYNLVGNLSEGLLAFQEKPDSKFGYINKEGNIVIKP 486 Query: 149 VYSFIAPFSEGRAVVSDAKG----YTLIDEKGKEVTPARADYLNSLQEGRALFSKQSTTG 204 ++ FSEGRA+V+ +K Y LID KGK V A+ + +N L EGR K Sbjct: 487 NFTQARAFSEGRAIVNTSKDYGNKYGLIDTKGKFVIEAKYNDINKLGEGRLAVGKAILED 546 Query: 205 K----SLYGYWDAQGKEVLPAVYEDAGDFAAGSALVKIKDGEYALIDPQGAVLHTYHHPF 260 K S Y D GK + +Y D+ G A D + ID G + + P Sbjct: 547 KPYIGSKYAIADNNGKFLTDFIYYGVSDYKNGLASA-YSDKDTFFIDKSGVRVKSL--PA 603 Query: 261 VGYPGDGLLAFQAE 274 V G G+L F+ + Sbjct: 604 VS--GSGILEFKKD 615 >B3K663.1 B3K663_9BACI KWG Leptospira repeat protein Length = 835 Score = 181 bits (459), Expect = 7e-44, Method: Compositional matrix adjust. Identities = 98/198 (49%), Positives = 125/198 (63%), Gaps = 4/198 (2%) Query: 87 LHPAPFKLKEGTLWGYINNEGKTAIEPRYDYAEEFQANGLAVVQRKDKSGLIDSTGREKV 146 L+PA K GT WGYI+ G I+P+Y+ A++FQ NGLAVV GLID R V Sbjct: 265 LYPASVKTTSGTKWGYIDRSGSMVIQPKYEDAQDFQDNGLAVVGADGLYGLIDRFARFVV 324 Query: 147 KPVYSFIAPFSEGRAVVSDAKGYTLIDEKGKEVTPARADYLNSLQEGRALFSKQSTTG-- 204 KP Y I+PF E RA V D +G+ +I+E+GK +T Y+ L EGRA+F + G Sbjct: 325 KPKYQSISPFLEHRATVIDRQGFQMINEQGKVLTRQSYPYIAGLSEGRAVFDVTDSGGGD 384 Query: 205 --KSLYGYWDAQGKEVLPAVYEDAGDFAAGSALVKIKDGEYALIDPQGAVLHTYHHPFVG 262 S YGY DA+G+E +PA YE+A DFA G A+VK+K+ YALIDPQG TY +VG Sbjct: 385 GANSRYGYLDAEGQEAIPAQYEEANDFAGGKAVVKVKENLYALIDPQGRQFATYPFAYVG 444 Query: 263 YPGDGLLAFQAEENGKYG 280 G+G+LAFQ E GKYG Sbjct: 445 PLGEGMLAFQKESAGKYG 462 Score = 58.9 bits (141), Expect = 4e-07, Method: Compositional matrix adjust. Identities = 48/144 (33%), Positives = 70/144 (48%), Gaps = 10/144 (6%) Query: 100 WGYINNEGKTAIEPRYDYAEEFQANGLAVVQRKDK-SGLIDSTGREKVKPVYSFIAPFSE 158 +GY++ EG+ AI +Y+ A +F A G AVV+ K+ LID GR+ ++++ P E Sbjct: 390 YGYLDAEGQEAIPAQYEEANDF-AGGKAVVKVKENLYALIDPQGRQFATYPFAYVGPLGE 448 Query: 159 GRAVVS--DAKGYTLIDEKGKEVTPARADYLNSLQEGRALFSKQSTTGKSLYGYWDAQGK 216 G A Y IDE+G V P GRA+ + S +S YG + +GK Sbjct: 449 GMLAFQKESAGKYGYIDEQGNVVIPPAYTSAFPFHRGRAIVNT-SEDYRSKYGVINVRGK 507 Query: 217 EVLPAVYEDAGD-----FAAGSAL 235 V+ Y D D FA G A+ Sbjct: 508 WVIQPEYNDIRDLKEDRFALGRAI 531 >B1KYA1.1 B1KYA1_CLOBM KWG leptospira repeat protein Length = 824 Score = 164 bits (415), Expect = 8e-39, Method: Compositional matrix adjust. Identities = 86/196 (43%), Positives = 123/196 (62%), Gaps = 2/196 (1%) Query: 87 LHPAPFKLKEGTLWGYINNEGKTAIEPRYDYAEEFQANGLAVVQRKDKSGLIDSTGREKV 146 L+P P +G WGYIN +G+ I+P Y+ A EFQ NGLAVV+ + G+ID V Sbjct: 258 LYPGPMNTIKGVRWGYINEKGEFLIKPLYNLALEFQCNGLAVVEIDNLYGIIDENQNYIV 317 Query: 147 KPVYSFIAPFSEGRAVVSDAKGYTLIDEKGKEVTPARAD--YLNSLQEGRALFSKQSTTG 204 K Y FI+ FSEGRA+V D + + +I+EKG+E+ P + Y+ + +EGRA + + Sbjct: 318 KHKYGFISDFSEGRAIVIDNERFNIINEKGEELIPKTENYSYIGNFKEGRAQYGVIDSNK 377 Query: 205 KSLYGYWDAQGKEVLPAVYEDAGDFAAGSALVKIKDGEYALIDPQGAVLHTYHHPFVGYP 264 LYGY D +GK +PA YE DF AG A+V+IK+ EYALI+ +G +L+ Y + VG Sbjct: 378 GYLYGYLDREGKVTIPAQYEYGNDFYAGKAVVRIKENEYALINIKGEILNKYEYASVGNL 437 Query: 265 GDGLLAFQAEENGKYG 280 +GLL+F+ + KYG Sbjct: 438 REGLLSFKKDMGEKYG 453 Score = 57.4 bits (137), Expect = 1e-06, Method: Compositional matrix adjust. Identities = 49/191 (25%), Positives = 82/191 (42%), Gaps = 11/191 (5%) Query: 96 EGTLWGYINNEGKTAIEPRYDYAEEFQANGLAVVQRKDKSGLIDSTGREKVKPVYSFIAP 155 +G L+GY++ EGK I +Y+Y +F A V ++++ LI+ G K Y+ + Sbjct: 377 KGYLYGYLDREGKVTIPAQYEYGNDFYAGKAVVRIKENEYALINIKGEILNKYEYASVGN 436 Query: 156 FSEGRAVVSDAKG--YTLIDEKGKEVTPARADYLNSLQEGRALFSKQSTTGKSLYGYWDA 213 EG G Y IDE G V Y EGR++ + + YG D Sbjct: 437 LREGLLSFKKDMGEKYGFIDEDGNVVIKPEFTYAQDFSEGRSVVNVSGNIMNN-YGVIDK 495 Query: 214 QGKEVLPAVYEDA-----GDFAAGSALVKIKD---GEYALIDPQGAVLHTYHHPFVGYPG 265 +G ++ Y D A G A+ K +YA+ D +G +L + + V Sbjct: 496 EGNYIITPKYNDIILLGENRVAVGVAIDKTSPFIGSKYAIADTEGNILTDFIYYGVSNYK 555 Query: 266 DGLLAFQAEEN 276 +G+ + ++N Sbjct: 556 NGIASAYDDKN 566 >C3L366.1 C3L366_CLOB6 KWG leptospira repeat protein Length = 824 Score = 161 bits (408), Expect = 5e-38, Method: Compositional matrix adjust. Identities = 85/196 (43%), Positives = 122/196 (62%), Gaps = 2/196 (1%) Query: 87 LHPAPFKLKEGTLWGYINNEGKTAIEPRYDYAEEFQANGLAVVQRKDKSGLIDSTGREKV 146 L+PAP +G WGYIN +G+ I+P Y+ A EFQ NGLAVV+ + G+ID V Sbjct: 258 LYPAPMNTIKGVRWGYINEKGEFLIKPLYNLALEFQCNGLAVVEIDNLYGIIDENQNYIV 317 Query: 147 KPVYSFIAPFSEGRAVVSDAKGYTLIDEKGKEVTPARAD--YLNSLQEGRALFSKQSTTG 204 K Y FI+ FSE RA+V D + + +I+EKG+E+ P + Y+ S +EGRA + + Sbjct: 318 KHKYGFISDFSEERAIVIDNERFNIINEKGEELIPKIENYSYIGSFKEGRAQYGVIDSNK 377 Query: 205 KSLYGYWDAQGKEVLPAVYEDAGDFAAGSALVKIKDGEYALIDPQGAVLHTYHHPFVGYP 264 LYGY D +G+ ++PA YE DF G A+V+IK+ EYALI+ G +L+ Y + VG Sbjct: 378 GYLYGYLDREGRVIIPAQYEYGNDFYEGKAVVRIKENEYALININGEILNKYEYASVGNL 437 Query: 265 GDGLLAFQAEENGKYG 280 +GLL+F+ + KYG Sbjct: 438 REGLLSFKKDMGEKYG 453 Score = 55.1 bits (131), Expect = 8e-06, Method: Compositional matrix adjust. Identities = 44/173 (25%), Positives = 74/173 (42%), Gaps = 11/173 (6%) Query: 96 EGTLWGYINNEGKTAIEPRYDYAEEFQANGLAVVQRKDKSGLIDSTGREKVKPVYSFIAP 155 +G L+GY++ EG+ I +Y+Y +F V ++++ LI+ G K Y+ + Sbjct: 377 KGYLYGYLDREGRVIIPAQYEYGNDFYEGKAVVRIKENEYALININGEILNKYEYASVGN 436 Query: 156 FSEGRAVVSDAKG--YTLIDEKGKEVTPARADYLNSLQEGRALFSKQSTTGKSLYGYWDA 213 EG G Y IDE G V + Y EGR++ + + YG D Sbjct: 437 LREGLLSFKKDMGEKYGFIDEDGNVVIKPQFTYAQDFSEGRSVVNVSGNIMNN-YGVIDK 495 Query: 214 QGKEVLPAVYEDA-----GDFAAGSALVKIKD---GEYALIDPQGAVLHTYHH 258 +G ++ Y D A G A+ K +YA+ D +G +L + + Sbjct: 496 EGNYIITPKYNDIILLGENRVAVGVAIDKTSPFIGSKYAIADTEGNILTDFIY 548 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.137 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,750,496,703 Number of extensions: 73110493 Number of successful extensions: 155051 Number of sequences better than 1.0e-05: 160 Number of HSP's gapped: 153730 Number of HSP's successfully gapped: 458 Length of query: 280 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 146 Effective length of database: 1,798,280,193 Effective search space: 262548908178 Effective search space used: 262548908178 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 130 (54.7 bits)