BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0348.0018.p (358 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q48FD6.1 Q48FD6_PSE14 Oxidoreductase, zinc-binding 277 1e-72 A7Z0T4.1 A7Z0T4_BACA2 GutB1 273 2e-71 Q889C0.1 Q889C0_PSESM Alcohol dehydrogenase, zinc-containing 269 4e-70 C3BU72.1 C3BU72_9BACI GutB1 266 3e-69 C1RWG9.1 C1RWG9_9SPHI Theronine dehydrogenase-like Zn-dependent ... 167 2e-39 >Q48FD6.1 Q48FD6_PSE14 Oxidoreductase, zinc-binding Length = 340 Score = 277 bits (708), Expect = 1e-72, Method: Compositional matrix adjust. Identities = 139/346 (40%), Positives = 208/346 (60%), Gaps = 16/346 (4%) Query: 14 MPAL-VWNSQRLNLSEQDIPVITLATDVKVKIQMTGICGTDLAVITGKEPGVPGVIRGHE 72 M AL + ++QRL L + PV+ V++ I ++GICGTDLAV++G+E G G+IRGHE Sbjct: 1 MKALGLMDNQRLELVDCKDPVMLAPDGVEIDIVLSGICGTDLAVLSGREGGEVGIIRGHE 60 Query: 73 AVGTVIETGSNVTSLKAGDRVVVDPNHSCGKCRFCLQGKLHLCTGTDGQGMPIAGLNKNG 132 AVG +I+ G V L+ G RVVVDPN CG C C K HLC G G+ IAG+NK+G Sbjct: 61 AVGIIIDVGEGVVHLQKGMRVVVDPNEYCGVCEPCRLAKTHLCNGAGNAGLDIAGVNKHG 120 Query: 133 TFAPWFVSEEKFLHKLPEQMSWEAALATEPLACVLHNFREGRVKTGDSVLILGSGPMGLL 192 TFA FV+ E+F+++LP+ MSW A + EP+AC+L+N + ++ G+ VLILGSGPM L+ Sbjct: 121 TFAERFVTRERFVYQLPDDMSWAAGVLVEPVACILNNIDQAFIRAGERVLILGSGPMSLI 180 Query: 193 CQFVAKRMGCTTVSIEINAGRL--AKAQQISDYACPPEELTAERVFRILGEKKFDFVIDT 250 Q V + MG T++ + N R+ ++Q + EL + ++KFD VIDT Sbjct: 181 AQIVLRSMGIDTLATDRNTHRIQFGRSQSLDVIHANDLELQMQH------QEKFDVVIDT 234 Query: 251 VGTQMEMAEQWVERGGTIIPFGINGRYQYTISPTYYTQHAVRLLGAGEYLNTFEDALQFA 310 VG Q++ A +++ RGG I+ FG + Y Y + Y+ +A+ ++ AGEY F A++ Sbjct: 235 VGNQIDTASRYIGRGGRIVLFGFDSDYHYMLPVKYFLINAISIISAGEYNQHFPRAIRLV 294 Query: 311 KDVPEVASLITRKYSLDQFETTIQELLGNSGNSAVSSEPPVLKSAF 356 + +PE+ L+T +Y L+ LL + + P +KS F Sbjct: 295 RKLPELGRLVTHRYVLENHSEVFDTLLND-------ASAPNIKSVF 333 >A7Z0T4.1 A7Z0T4_BACA2 GutB1 Length = 348 Score = 273 bits (698), Expect = 2e-71, Method: Compositional matrix adjust. Identities = 140/327 (42%), Positives = 198/327 (60%), Gaps = 6/327 (1%) Query: 14 MPALVWN-SQRLNLSEQDIPVITLATDVKVKIQMTGICGTDLAVITGKEPGVPGVIRGHE 72 M ALVW + +L E D P I + DVKVKI TGICGTDL V+ GK +I GHE Sbjct: 1 MKALVWTPNDKLEYQEVDEPQIRKSNDVKVKIFGTGICGTDLNVLKGKMNATHHMIMGHE 60 Query: 73 AVGTVIETGSNVTSLKAGDRVVVDPNHSCGKCRFCLQGKLHLC-TGTDGQGMPIAGLNKN 131 +VG V+E G +VT++K GDRVV+DP CGKC +C +G C T D Q G+ + Sbjct: 61 SVGAVVEIGPDVTNVKVGDRVVIDPTQFCGKCHYCRRGLTCYCETFEDWQ----LGIGAH 116 Query: 132 GTFAPWFVSEEKFLHKLPEQMSWEAALATEPLACVLHNFREGRVKTGDSVLILGSGPMGL 191 GTFA ++V E++F++++P+ M WE A EPL+CVL+ + ++ DSVL+LGSGP+GL Sbjct: 117 GTFAEYYVGEDRFMYRIPDSMEWERATLVEPLSCVLNVVDKASIQPEDSVLVLGSGPIGL 176 Query: 192 LCQFVAKRMGCTTVSIEINAGRLAKAQQISDYACPPEELTAERVFRILGEKKFDFVIDTV 251 L Q + K++ TV+ EI + R A +ISDY PE LT + V RI +KFD + D + Sbjct: 177 LVQMMVKKLARLTVATEIGSFRSEAASRISDYVYHPEALTVDEVKRINQGRKFDVIFDAI 236 Query: 252 GTQMEMAEQWVERGGTIIPFGINGRYQYTISPTYYTQHAVRLLGAGEYLNTFEDALQFAK 311 G Q++ A ++E+GG I+P G + Y+ T+ P + V ++G GE E AL A Sbjct: 237 GNQLDWAYPFIEKGGRIVPMGFDDTYEMTVRPYQLLSNGVTIVGTGEARQIMEAALACAS 296 Query: 312 DVPEVASLITRKYSLDQFETTIQELLG 338 D+P++ IT K L +E I L+G Sbjct: 297 DLPQLYDFITEKTELKNYEEAIDHLMG 323 >Q889C0.1 Q889C0_PSESM Alcohol dehydrogenase, zinc-containing Length = 319 Score = 269 bits (687), Expect = 4e-70, Method: Compositional matrix adjust. Identities = 133/324 (41%), Positives = 198/324 (61%), Gaps = 16/324 (4%) Query: 36 LATD-VKVKIQMTGICGTDLAVITGKEPGVPGVIRGHEAVGTVIETGSNVTSLKAGDRVV 94 LA D V++ I ++GICGTDLAV++G+E G G+IRGHEAVG +I+ G +V L+ G RVV Sbjct: 2 LAPDGVEIDIVLSGICGTDLAVLSGREGGEVGIIRGHEAVGIIIDVGKDVVHLQKGMRVV 61 Query: 95 VDPNHSCGKCRFCLQGKLHLCTGTDGQGMPIAGLNKNGTFAPWFVSEEKFLHKLPEQMSW 154 VDPN CG C C K HLC G G+ IAG+NK+GTFA FV+ E+F+++LP+ MSW Sbjct: 62 VDPNEYCGVCEPCRLAKTHLCNGGVNAGLDIAGVNKHGTFAERFVTRERFVYQLPDDMSW 121 Query: 155 EAALATEPLACVLHNFREGRVKTGDSVLILGSGPMGLLCQFVAKRMGCTTVSIEINAGRL 214 A + EP+AC+L+N + ++ G+ VLILGSGPM L+ Q V + MG T++ + N R+ Sbjct: 122 AAGVLVEPVACILNNIDQAFIRAGERVLILGSGPMSLIAQIVLRSMGVDTLATDRNTHRI 181 Query: 215 --AKAQQISDYACPPEELTAERVFRILGEKKFDFVIDTVGTQMEMAEQWVERGGTIIPFG 272 ++Q + EL + ++KFD VIDTVG Q++ A +++ RGG I+ FG Sbjct: 182 QFGRSQSLDVIHADDLELQMQH------QEKFDVVIDTVGNQIDTASRYIGRGGRIVLFG 235 Query: 273 INGRYQYTISPTYYTQHAVRLLGAGEYLNTFEDALQFAKDVPEVASLITRKYSLDQFETT 332 + Y Y + Y+ +A+ ++ AGEY F A++ + +PE+ L+T +Y L+ Sbjct: 236 FDSDYHYMLPVKYFLVNAISIISAGEYNQHFPRAIRLVQKLPELGRLVTHRYVLENHSEV 295 Query: 333 IQELLGNSGNSAVSSEPPVLKSAF 356 LL + + P +KS F Sbjct: 296 FDALLND-------ASAPNIKSVF 312 >C3BU72.1 C3BU72_9BACI GutB1 Length = 332 Score = 266 bits (679), Expect = 3e-69, Method: Compositional matrix adjust. Identities = 132/329 (40%), Positives = 195/329 (59%), Gaps = 14/329 (4%) Query: 32 PVITLATDVKVKIQMTGICGTDLAVITGKEPGVPGVIRGHEAVGTVIETGSNVTSLKAGD 91 P I DVKV+I TGICGTDL V+ GK P ++ GHE+VG V+E G V ++ GD Sbjct: 4 PQIQKNNDVKVRIYGTGICGTDLNVLKGKMYAAPNMVMGHESVGVVVEIGEEVRNVTVGD 63 Query: 92 RVVVDPNHSCGKCRFCLQGKLHLCTGTDGQGMPIAGLNKNGTFAPWFVSEEKFLHKLPEQ 151 RVV+DP CGKC +C +G C D + G+ +GTF ++V E++F++KLPE Sbjct: 64 RVVIDPTQFCGKCYYCRKGLTCYCEEFDEWQL---GIGSHGTFGEYYVGEDRFIYKLPES 120 Query: 152 MSWEAALATEPLACVLHNFREGRVKTGDSVLILGSGPMGLLCQFVAKRMGCTTVSIEINA 211 M WE A EPL+CVL + +K DSVL+LGSGP+GLL Q + +++ TV+ EI Sbjct: 121 MEWERATLIEPLSCVLSVIEKANIKPDDSVLVLGSGPIGLLVQMITRKLSRITVATEIGP 180 Query: 212 GRLAKAQQISDYACPPEELTAERVFRILGEKKFDFVIDTVGTQMEMAEQWVERGGTIIPF 271 R A++I+ Y P++LT E ++RI +KFD + D +G Q++ A +E+GG +IP Sbjct: 181 FRTEAARKITKYVYHPQKLTLEEIYRINQGRKFDVIFDAIGNQLDWAYPLIEKGGRLIPM 240 Query: 272 GINGRYQYTISPTYYTQHAVRLLGAGEYLNTFEDALQFAKDVPEVASLITRKYSLDQFET 331 G + Y++ + P + ++G GE + E AL A D+PE+++LIT K +L+ F+T Sbjct: 241 GFDDTYEFKVKPFELLSKGITIIGTGEAHHMMEKALSCAIDLPELSNLITEKVALENFDT 300 Query: 332 TIQELLG-----------NSGNSAVSSEP 349 I L+G NS + + SEP Sbjct: 301 AIGNLMGFDPTTNERKDINSIKTILVSEP 329 >C1RWG9.1 C1RWG9_9SPHI Theronine dehydrogenase-like Zn-dependent dehydrogenase Length = 340 Score = 167 bits (422), Expect = 2e-39, Method: Compositional matrix adjust. Identities = 107/347 (30%), Positives = 182/347 (52%), Gaps = 9/347 (2%) Query: 14 MPALVWNSQ-RLNLSEQDIPVITLATDVKVKIQMTGICGTDLAVITGKEPGVPGVIRGHE 72 M A+V+N + L E++ P ITL +V V+I+ TGICGTDL++I+G+ VI GHE Sbjct: 1 MQAIVYNGPWNIQLEEREEPAITLPDEVIVEIRATGICGTDLSIISGEYNARQQVIIGHE 60 Query: 73 AVGTVIETGSNVTSLKAGDRVVVDPNHSCGKCRFCLQGKLHLCTGTDGQGMPIAGLNKNG 132 + G ++E GS V + GDRV++DP + CG C C +G + C + AG++ +G Sbjct: 61 SAGVIVEKGSGVENFNVGDRVIIDPTYYCGYCENCRKGLRNHCF---QKSSTEAGVSIDG 117 Query: 133 TFAPWFVSEEKFLHKLPEQMSWEAALATEPLACVLHNFREGRVKTGDSVLILGSGPMGLL 192 TF +F + ++F++ L + + +E +EPL+CVL ++ V +V ILG GP+GLL Sbjct: 118 TFTKYFKTTKRFIYPLGDNVPFEQGAMSEPLSCVLTAVKKLAVSPFYNVAILGGGPIGLL 177 Query: 193 CQFVAKRMGCTTVSIEINAGRLAKAQQISDYACPPEELTAERVFRILGEKKFDFVIDTVG 252 K+ G +I + +D L+ R + ++D ++DT G Sbjct: 178 FYLALKQYGIQAGTIYETSANRTNLISSNDILSSGWYLSGHFAPR---KHQYDLIVDTTG 234 Query: 253 TQMEMAEQWVERGGTIIPFGINGRYQYTISPTYYTQHAVRLLGAGEYLNTFEDALQFAKD 312 + +E + V GG I G+ Q TI+P T ++ ++G+ + +TF+ A+ Sbjct: 235 SMLEKSIDAVADGGKIALIGLRNN-QQTINPREITDRSISIIGSIDSQDTFKHAVDLINS 293 Query: 313 -VPEVASLITRKYSLDQFETTIQELLGNSGNSAVSSEPPVLKSAFVF 358 V + +IT+ Y +D+F+ + +L N +++ LKS F Sbjct: 294 QVLGLEKIITKAYGIDEFQDAVLDLGCNVSKRQRNNDISSLKSVIKF 340 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.135 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,165,634,899 Number of extensions: 91698603 Number of successful extensions: 297537 Number of sequences better than 1.0e-05: 8327 Number of HSP's gapped: 286002 Number of HSP's successfully gapped: 8335 Length of query: 358 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 221 Effective length of database: 1,770,017,148 Effective search space: 391173789708 Effective search space used: 391173789708 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)