BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0348.0017.p (245 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B9YDG1.1 B9YDG1_9FIRM Putative uncharacterized protein 165 4e-39 A7HM58.1 A7HM58_FERNB Alpha amylase catalytic region 162 2e-38 B7IGT4.1 B7IGT4_THEAB Maltodextrin glycosyltransferase 160 1e-37 Q9S5X2.1 Q9S5X2_THEMA Maltodextrin glycosyltransferase 156 1e-36 O33838.1 O33838_THEMA Maltodextrin glycosyltransferase 156 1e-36 >B9YDG1.1 B9YDG1_9FIRM Putative uncharacterized protein Length = 693 Score = 165 bits (417), Expect = 4e-39, Method: Compositional matrix adjust. Identities = 97/232 (41%), Positives = 135/232 (58%), Gaps = 26/232 (11%) Query: 7 DCRFHNTAPIWIPALWNECGYVSTLDSKDGEILVEPARFMVKHLQYLLELSSD------Y 60 D F+ T P +L +C L S GEI V P +F + + LL+ S Y Sbjct: 19 DYPFNYTLPDLFDSLDLKCTSKYKLPS--GEIRVNPYQFFDELINVLLKGKSKPVCQPYY 76 Query: 61 A--HIGQAD-----LDDSVIYCSLLRYSSAWDYTHTGNIQS---------GTFLKFMILL 104 A + Q + S +Y ++R SS+WD+ +G ++S GTF+K + LL Sbjct: 77 ADHSLPQGGTRGNWIRQSSVYSCMIRTSSSWDHDRSGELESKNIYGLKETGTFVKTLALL 136 Query: 105 PLLKKMNVNILYLLPVTRYSNLNLKGDIGSPYAVYNLFDLDENLHDPLLDGMENLTIHDE 164 PLLKKM ++ LYLLP+++YS N KGD+GSPY V N F LD NL DP+ + L++ DE Sbjct: 137 PLLKKMGIDTLYLLPISQYSTKNKKGDLGSPYGVSNFFKLDPNLKDPMTG--DELSVEDE 194 Query: 165 LATLVEACHLLDIKVVLDLIPRVGARDNVLLAEHPDWFYWIDLNALPGFQPP 216 LVEACH DIKV++DLIPR + ++ L+AEHPDWFYWI+ + L + PP Sbjct: 195 FKALVEACHCQDIKVIIDLIPRTNSVNSDLIAEHPDWFYWIEADQLDTYTPP 246 >A7HM58.1 A7HM58_FERNB Alpha amylase catalytic region Length = 669 Score = 162 bits (411), Expect = 2e-38, Method: Compositional matrix adjust. Identities = 87/218 (39%), Positives = 128/218 (58%), Gaps = 22/218 (10%) Query: 18 IPALWNECGYVSTLDSKDGEILVEPARFMVKHLQYLLELSS--DYAHI-----GQAD--- 67 +P W Y + KDG V+P + + Y+LE DY+ G+ Sbjct: 23 LPKQWFISHYAGKVSIKDGRYFVDPYEYFAFLIDYILENDKGFDYSKSLAMLNGEVKPIW 82 Query: 68 LDDSVIYCSLLRYSSAWDYTHTGNIQ---------SGTFLKFMILLPLLKKMNVNILYLL 118 + IY +L R ++A+++ G + SGTFLK + LLP LK M +NILY+L Sbjct: 83 IGKGKIYGALPRATAAYNHKGFGAFEPEDIFGYKESGTFLKMIALLPYLKNMGINILYML 142 Query: 119 PVTRYSNLNLKGDIGSPYAVYNLFDLDENLHDPLLDGMENLTIHDELATLVEACHLLDIK 178 P+++ SN+ KG++GSPYAV N +LDE+ HDPLL+G ++I ++ LV+A H+L I+ Sbjct: 143 PISKMSNIFKKGEVGSPYAVKNPVELDESYHDPLLEG---ISIDEQFKALVQAAHMLGIR 199 Query: 179 VVLDLIPRVGARDNVLLAEHPDWFYWIDLNALPGFQPP 216 VVLD IPR ARD+ L+ EHPDWFYWI + + ++PP Sbjct: 200 VVLDFIPRTAARDSDLIKEHPDWFYWIRIEEVASYKPP 237 >B7IGT4.1 B7IGT4_THEAB Maltodextrin glycosyltransferase Length = 666 Score = 160 bits (405), Expect = 1e-37, Method: Compositional matrix adjust. Identities = 92/220 (41%), Positives = 127/220 (57%), Gaps = 24/220 (10%) Query: 18 IPALW---NECGYVSTLDSKDGEILVEPARFMVKHLQYL-------LELSSDYAHI-GQA 66 IP LW N+ + + + K I V+P F K + Y+ + S ++I G+ Sbjct: 21 IPKLWIHSNKEIFGNIFEEKGNTIFVDPYEFFYKVVSYINSQRKEKVNYSKSISNITGEK 80 Query: 67 DL-DDSVIYCSLLRYSSAWDYTHTGNIQ---------SGTFLKFMILLPLLKKMNVNILY 116 D ++IY SL R + A+++ N + SGTFLK + L LK NVN LY Sbjct: 81 DWHKKAIIYGSLPRMTVAYNHKGFANFEETDILGFKESGTFLKMIAFLIYLKHFNVNTLY 140 Query: 117 LLPVTRYSNLNLKGDIGSPYAVYNLFDLDENLHDPLLDGMENLTIHDELATLVEACHLLD 176 +LP+++ SNL KG IGSPYAV N LDEN HDPLL+G + +E LVEA H+L Sbjct: 141 MLPISQSSNLFKKGSIGSPYAVKNPLKLDENYHDPLLEG---FNVEEEFQALVEAAHILG 197 Query: 177 IKVVLDLIPRVGARDNVLLAEHPDWFYWIDLNALPGFQPP 216 I+VVLD IPR +RD+ L+ EHPDWFYWI ++ L ++PP Sbjct: 198 IRVVLDFIPRTASRDSDLIREHPDWFYWIKVDELSEYKPP 237 >Q9S5X2.1 Q9S5X2_THEMA Maltodextrin glycosyltransferase Length = 639 Score = 156 bits (395), Expect = 1e-36, Method: Compositional matrix adjust. Identities = 88/227 (38%), Positives = 124/227 (54%), Gaps = 28/227 (12%) Query: 9 RFHNTAPIWIPALWNECGYVSTLDSKDGEILVEPARFMVKHLQYLLELSS--DYAH---- 62 R + +WIP G+ D K G V+P + ++L S DY+ Sbjct: 20 RIYAVPKLWIP------GFFKKFDEKSGRCFVDPYELGAEITDWILNQSREWDYSQPLSF 73 Query: 63 -IGQADLD---DSVIYCSLLRYSSAWDYTHTGNIQ---------SGTFLKFMILLPLLKK 109 G+ D SV+Y SL R ++A+++ +G + +GTF K M+LLP +K Sbjct: 74 LKGEKTPDWIKRSVVYGSLPRTTAAYNHKGSGYYEENDVLGFREAGTFFKMMLLLPFVKS 133 Query: 110 MNVNILYLLPVTRYSNLNLKGDIGSPYAVYNLFDLDENLHDPLLDGMENLTIHDELATLV 169 + + +YLLPV+R S+L KGD SPY+V N +LDE HDPLL E + +E V Sbjct: 134 LGADAIYLLPVSRMSDLFKKGDAPSPYSVKNPMELDERYHDPLL---EPFKVDEEFKAFV 190 Query: 170 EACHLLDIKVVLDLIPRVGARDNVLLAEHPDWFYWIDLNALPGFQPP 216 EACH+L I+V+LD IPR ARD+ L+ EHPDWFYWI + L + PP Sbjct: 191 EACHILGIRVILDFIPRTAARDSDLIREHPDWFYWIKVEELADYTPP 237 >O33838.1 O33838_THEMA Maltodextrin glycosyltransferase Length = 637 Score = 156 bits (395), Expect = 1e-36, Method: Compositional matrix adjust. Identities = 88/227 (38%), Positives = 124/227 (54%), Gaps = 28/227 (12%) Query: 9 RFHNTAPIWIPALWNECGYVSTLDSKDGEILVEPARFMVKHLQYLLELSS--DYAH---- 62 R + +WIP G+ D K G V+P + ++L S DY+ Sbjct: 18 RIYAVPKLWIP------GFFKKFDEKSGRCFVDPYELGAEITDWILNQSREWDYSQPLSF 71 Query: 63 -IGQADLD---DSVIYCSLLRYSSAWDYTHTGNIQ---------SGTFLKFMILLPLLKK 109 G+ D SV+Y SL R ++A+++ +G + +GTF K M+LLP +K Sbjct: 72 LKGEKTPDWIKRSVVYGSLPRTTAAYNHKGSGYYEENDVLGFREAGTFFKMMLLLPFVKS 131 Query: 110 MNVNILYLLPVTRYSNLNLKGDIGSPYAVYNLFDLDENLHDPLLDGMENLTIHDELATLV 169 + + +YLLPV+R S+L KGD SPY+V N +LDE HDPLL E + +E V Sbjct: 132 LGADAIYLLPVSRMSDLFKKGDAPSPYSVKNPMELDERYHDPLL---EPFKVDEEFKAFV 188 Query: 170 EACHLLDIKVVLDLIPRVGARDNVLLAEHPDWFYWIDLNALPGFQPP 216 EACH+L I+V+LD IPR ARD+ L+ EHPDWFYWI + L + PP Sbjct: 189 EACHILGIRVILDFIPRTAARDSDLIREHPDWFYWIKVEELADYTPP 235 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.323 0.141 0.454 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,559,169,630 Number of extensions: 62955347 Number of successful extensions: 127495 Number of sequences better than 1.0e-05: 59 Number of HSP's gapped: 127608 Number of HSP's successfully gapped: 59 Length of query: 245 Length of database: 3,060,696,203 Length adjustment: 132 Effective length of query: 113 Effective length of database: 1,817,122,223 Effective search space: 205334811199 Effective search space used: 205334811199 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 129 (54.3 bits)