BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0348.0016.p (307 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A9BFM9.1 A9BFM9_PETMO Alpha amylase catalytic sub domain 156 3e-36 B9MLT9.1 B9MLT9_ANATD Alpha amylase catalytic sub domain protein 151 8e-35 Q9S5X2.1 Q9S5X2_THEMA Maltodextrin glycosyltransferase 148 7e-34 O33838.1 O33838_THEMA Maltodextrin glycosyltransferase 146 2e-33 A4XG16.1 A4XG16_CALS8 Alpha amylase, catalytic sub domain 145 4e-33 >A9BFM9.1 A9BFM9_PETMO Alpha amylase catalytic sub domain Length = 676 Score = 156 bits (394), Expect = 3e-36, Method: Compositional matrix adjust. Identities = 86/239 (35%), Positives = 135/239 (56%), Gaps = 14/239 (5%) Query: 1 VLFDTIKCNIYPGEEPNQELWDTLIEAASFQLKTYNIDGFRVDIGHTLPVPLLQSMFKMI 60 VLFD +K +++PG+E N ELW+ + F K + IDG R+D+GH LP L + K Sbjct: 352 VLFDVVKSSLFPGKEKNAELWNLIANIIPFYQKNFGIDGARLDMGHALPDELEAEIIKRA 411 Query: 61 KDMKPAAILISEDLFNRNHREAAKTGYNVMLGSGWNIMTDIRVERLQDYLREL-PELQIH 119 K+ PA ++I+E+L NH +A K+GY+ +LG+ W I+ + ++ E+ P+L + Sbjct: 412 KEYDPAFVIIAEELNMDNHLKAKKSGYDGILGNSWWAEPRIKEGWFKKFISEVAPKLSLP 471 Query: 120 VFACSETADTPRITSRGGTE-LARLMTVFNYFLPNGIPYLTTGMEVYEEQPLNCGLG--- 175 FA +ET DTPR +R G E ++L V N F+PNGI ++ +G E++E QP+N GL Sbjct: 472 TFATAETPDTPRAVTREGEETFSKLTAVVNTFMPNGITFINSGYELFEPQPMNTGLDVED 531 Query: 176 ------DNTGGADI---PKAFFNTMAINWASKSTLLPLLEQLQQMKRQHANLLKPANFY 225 N D AFF+ ++W + ++ LL L +K + +LLK +N Y Sbjct: 532 PVEERFKNLPSTDQFYGKLAFFDWYVLHWDTDHHMVKLLSLLGTLKEEVKDLLKNSNNY 590 >B9MLT9.1 B9MLT9_ANATD Alpha amylase catalytic sub domain protein Length = 692 Score = 151 bits (381), Expect = 8e-35, Method: Compositional matrix adjust. Identities = 81/256 (31%), Positives = 140/256 (54%), Gaps = 13/256 (5%) Query: 1 VLFDTIKCNIYPGEEPNQELWDTLIEAASFQLKTYNIDGFRVDIGHTLPVPLLQSMFKMI 60 +LFD I+ NI+ G++PN++LW+ + + K + IDG R+D+GH LP L + Sbjct: 365 ILFDVIRGNIFKGKKPNKKLWEKIANIIVYYQKNFGIDGARIDMGHALPKELEDMIISNA 424 Query: 61 KDMKPAAILISEDLFNRNHREAAKTGYNVMLGSGWNIMTDIRVERLQDYLRELPELQIHV 120 + + I+E+L HR+A ++GY++++G W L+ L L +L++ V Sbjct: 425 RKVDTEFCFIAEELSMNAHRKAKESGYDMIIGDLWAREPRYYQGTLKKVLDILLQLEVPV 484 Query: 121 FACSETADTPRITSR-GGTELARLMTVFNYFLPNGIPYLTTGMEVYEEQPLNCGLGDNTG 179 FA E D+PR SR G E +R TV N+FLPN +P++T G EVYE QP+N GL Sbjct: 485 FAACEIPDSPRAASRLGKREFSRFATVLNFFLPNSVPFVTCGQEVYEVQPMNLGLDPQPE 544 Query: 180 GADIPK---------AFFNTMAINWASKST--LLPLLEQLQQMKRQHANLLKPANFY-LS 227 G + AFF+ A++W ++ ++ L+E + ++++++ + + NF+ + Sbjct: 545 GKFMLSKSDPLYGKLAFFDKYALHWTNEGADEMIALIEGVAKIRKEYLDFMSKENFFKIP 604 Query: 228 DSQGDILIYSYYLKDE 243 + +L + Y L E Sbjct: 605 HNSKFVLAFGYKLFSE 620 >Q9S5X2.1 Q9S5X2_THEMA Maltodextrin glycosyltransferase Length = 639 Score = 148 bits (373), Expect = 7e-34, Method: Compositional matrix adjust. Identities = 86/249 (34%), Positives = 137/249 (55%), Gaps = 14/249 (5%) Query: 1 VLFDTIKCNIYPGEEPNQELWDTLIEAASFQLKTYNIDGFRVDIGHTLPVPLLQSMFKMI 60 VL+D IK + +PG+EPN+ELW+ L K Y IDG R+D+GH LP LL + K + Sbjct: 345 VLYDVIKASKFPGKEPNRELWEYLAGVIPHYQKKYGIDGARLDMGHALPKELLDLIIKNV 404 Query: 61 KDMKPAAILISEDLFNRNHREAAKTGYNVMLGSGWNIMTDIRVERLQDYLRELPELQIHV 120 K+ PA ++I+E+L + + + GY+V+LGS W RVE + EL + Sbjct: 405 KEYDPAFVMIAEELDMEKDKASKEAGYDVILGSSWYFAG--RVEEIGKLPDIAEELVLPF 462 Query: 121 FACSETADTPRITSRG-GTELARLMTVFNYFLPNGIPYLTTGMEVYEEQPLNCGLGDNTG 179 A ET DTPRI +R +++ +L YFLPN IPY+ TG E+ E+QP+N GL + Sbjct: 463 LASVETPDTPRIATRKYASKMKKLAPFVTYFLPNSIPYVNTGQEIGEKQPMNLGLDTDPN 522 Query: 180 GADIPK---------AFFNTMAINWASKST-LLPLLEQLQQMKRQHANLLKPANFYLSDS 229 + AFF+ ++W S +L +++L ++++Q + + F + + Sbjct: 523 LRKVLSPTDEFFGKLAFFDHYVLHWDSPDRGVLNFIKKLIKVRQQFLDFVLNGKFE-NLT 581 Query: 230 QGDILIYSY 238 D+++YSY Sbjct: 582 TKDLVMYSY 590 >O33838.1 O33838_THEMA Maltodextrin glycosyltransferase Length = 637 Score = 146 bits (369), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 85/249 (34%), Positives = 136/249 (54%), Gaps = 14/249 (5%) Query: 1 VLFDTIKCNIYPGEEPNQELWDTLIEAASFQLKTYNIDGFRVDIGHTLPVPLLQSMFKMI 60 VL+D IK + +PG+EPN+ELW+ L K Y IDG R+D+GH LP LL + K + Sbjct: 343 VLYDVIKASKFPGKEPNRELWEYLAGVIPHYQKKYGIDGARLDMGHALPKELLDLIIKNV 402 Query: 61 KDMKPAAILISEDLFNRNHREAAKTGYNVMLGSGWNIMTDIRVERLQDYLRELPELQIHV 120 K+ PA ++I+E+L + + + GY+V+LGS W RVE + EL + Sbjct: 403 KEYDPAFVMIAEELDMEKDKASKEAGYDVILGSSWYFAG--RVEEIGKLPDIAEELVLPF 460 Query: 121 FACSETADTPRITSRG-GTELARLMTVFNYFLPNGIPYLTTGMEVYEEQPLNCGLGDNTG 179 A ET DTPRI +R +++ +L YFLPN IPY+ TG E+ E+QP+N GL + Sbjct: 461 LASVETPDTPRIATRKYASKMKKLAPFVTYFLPNSIPYVNTGQEIGEKQPMNLGLDTDPN 520 Query: 180 GADIPK---------AFFNTMAINWASKST-LLPLLEQLQQMKRQHANLLKPANFYLSDS 229 + AFF+ ++W S +L +++L +++ + + + F + + Sbjct: 521 LRKVLSPTDEFFGKLAFFDHYVLHWDSPDRGVLNFIKKLIKVRHEFLDFVLNGKFE-NLT 579 Query: 230 QGDILIYSY 238 D+++YSY Sbjct: 580 TKDLVMYSY 588 >A4XG16.1 A4XG16_CALS8 Alpha amylase, catalytic sub domain Length = 692 Score = 145 bits (367), Expect = 4e-33, Method: Compositional matrix adjust. Identities = 81/260 (31%), Positives = 140/260 (53%), Gaps = 15/260 (5%) Query: 1 VLFDTIKCNIYPGEEPNQELWDTLIEAASFQLKTYNIDGFRVDIGHTLPVPLLQSMFKMI 60 +LFDTI+ NI+ G++PN ELW+ + + + IDG R+D+GH LP L + Sbjct: 366 ILFDTIRANIFKGKKPNIELWERISNIIVHYQQRFGIDGARIDMGHALPKELEDMIITKA 425 Query: 61 KDMKPAAILISEDLFNRNHREAAKTGYNVMLGSGWNIMTDIRVERLQDYLRELPELQIHV 120 K++ P I+E+L ++A ++GY++++G W L+ + +L +L++ V Sbjct: 426 KEVDPDFCFIAEELSLSGDKKAKESGYDMIIGDVWAREPRYYEGNLKKMIDKLLKLKLPV 485 Query: 121 FACSETADTPRITSR-GGTELARLMTVFNYFLPNGIPYLTTGMEVYEEQPLNCGLGDNTG 179 FA SE D+PR SR GG +R V N FLPN + +LT+G EV+E QP+N GL Sbjct: 486 FAASEIPDSPRAASRVGGKNFSRFSVVLNNFLPNAVFFLTSGQEVFEVQPMNLGLDPQPD 545 Query: 180 GA-DIPK--------AFFNTMAINWASKST--LLPLLEQLQQMKRQHANLLKPANFYLSD 228 G + K AFF+ A++W + ++ ++ + ++++++ + +KP N+ Sbjct: 546 GRFKLSKSDPLYGKLAFFDRYALHWTNDGAQEMIEIISAVSKIRKRYVDFIKPENYIKFP 605 Query: 229 SQGDILI---YSYYLKDELF 245 ++ Y LKDE + Sbjct: 606 YNSKFVVAFGYKLELKDEKY 625 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.137 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,898,498,913 Number of extensions: 78913079 Number of successful extensions: 165404 Number of sequences better than 1.0e-05: 31 Number of HSP's gapped: 165370 Number of HSP's successfully gapped: 31 Length of query: 307 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 172 Effective length of database: 1,788,859,178 Effective search space: 307683778616 Effective search space used: 307683778616 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)