BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0348.0013.p (453 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A6TVU2.1 A6TVU2_ALKMQ MATE efflux family protein 300 2e-79 Q97KS0.1 Q97KS0_CLOAB Probable cation efflux pump (Multidrug res... 291 7e-77 C4ICG8.1 C4ICG8_CLOBU Mate efflux family protein 291 1e-76 B1QUL4.1 B1QUL4_CLOBU Putative Na+ driven multidrug efflux pump 291 1e-76 A9BJ76.1 A9BJ76_PETMO MATE efflux family protein 273 3e-71 >A6TVU2.1 A6TVU2_ALKMQ MATE efflux family protein Length = 453 Score = 300 bits (767), Expect = 2e-79, Method: Compositional matrix adjust. Identities = 158/443 (35%), Positives = 254/443 (57%) Query: 9 FYSKIYTIAIPVTLQSLIMALLTLTDQLMVGQLGDVAVASVGISIKIYGIITVVLAGLAT 68 FY + IA+P+T+Q+LI + L D +M+G LG++ +A+VG++ + + + ++L G+ + Sbjct: 11 FYKTMLAIALPITIQNLISSALNTVDTIMIGNLGELEIAAVGLANQFFFMFVLLLFGINS 70 Query: 69 GVSIYAAQFWGKKDEKSTAQXXXXXXXXXXXISIIFTLPVHFFPQFCLGLFTKDQRLITE 128 G SI+ +QFWG++D K+ + +S++F + P LG FT DQ +I Sbjct: 71 GASIFISQFWGRQDIKNIHRVLGIALISGGILSLVFAVGAFGVPNVILGFFTHDQSVIDL 130 Query: 129 GDIFLEITSLSYIPVMLTMIFSAILRATGHVKLPMMTSIVAVCINIVLNYLLIFGNFGFP 188 G +L I SLSY +T +S R+TG KLPM+ S V++ IN VLNY+ IFG FG P Sbjct: 131 GSQYLRIVSLSYFATAVTFAYSFAARSTGEAKLPMVVSAVSLSINTVLNYIYIFGKFGAP 190 Query: 189 EWGIKGAATSTLIARLFECCFIIAAAYKLRLPGVTHIRDWFGAPTALLSKFIRTTYPIVL 248 G+ GAA +TL AR E ++ Y + I+D + K+ +T YP++L Sbjct: 191 ALGVSGAAWATLAARSVEMVLLLGMVYGKKYAIAGKIKDMMNLTREFVLKYFKTAYPVIL 250 Query: 249 TELIWVLGETVYAIIYSRMGTVEITAMTITYPLQXXXXXXXXXXXXXXXXMIGNKLGADE 308 E W LG T+Y IY+R+ T I ++ I +Q M+GN++G + Sbjct: 251 NEGFWSLGMTMYTAIYARISTEAIASVQIANTVQSIFMVVGMGLGNACAVMLGNEIGGNH 310 Query: 309 QDAALDFSKRFIKLGIFVSLGIGVLIAAIAPLYVSIFQVSEEAHHMGTRLVWVFAGFLWV 368 ++ A +++K+F LG +G+L+ A+AP + +F VS E H R++ V F+ Sbjct: 311 KERAFNYAKKFAILGPLTGGIMGMLLFALAPYILKLFNVSTEVHINAIRILMVLGVFMAF 370 Query: 369 KVANMILAGGILNSGGDSKFVFAMEAAATWLIGVPSGLLMSFVWKQPVFLVYMILSLEEV 428 K+ N IL GI+ SGGD+KF +E + WLIGVP L + VW+ P++ V +++S+EEV Sbjct: 371 KIFNAILVIGIVRSGGDTKFSLFLELGSVWLIGVPLAFLGAHVWQLPIYWVVVLVSMEEV 430 Query: 429 VRLSIGLARIHSRKWMKNLVAEI 451 V+ IG R+ S+KW+ N+V ++ Sbjct: 431 VKAGIGSVRLISKKWIHNVVEQM 453 >Q97KS0.1 Q97KS0_CLOAB Probable cation efflux pump (Multidrug resistance protein) Length = 459 Score = 291 bits (746), Expect = 7e-77, Method: Compositional matrix adjust. Identities = 152/452 (33%), Positives = 253/452 (55%), Gaps = 2/452 (0%) Query: 1 MLKDSKADFYSKIYTIAIPVTLQSLIMALLTLTDQLMVGQLGDVAVASVGISIKIYGIIT 60 + D K F+ + +AIP+ +Q+ I++ L L D +++G LG+ A+A VG++ + + ++ Sbjct: 6 LFNDKK--FFKAAFGLAIPIIIQNFILSSLNLVDNIIIGGLGETAIAGVGLANQYFFLLD 63 Query: 61 VVLAGLATGVSIYAAQFWGKKDEKSTAQXXXXXXXXXXXISIIFTLPVHFFPQFCLGLFT 120 + L G A+G SI+ AQ+WGK+D K+ + +S IFT+ FP +G F+ Sbjct: 64 LALFGTASGASIFTAQYWGKRDTKNIKRVLGICLITTIILSGIFTIGALAFPNIIIGFFS 123 Query: 121 KDQRLITEGDIFLEITSLSYIPVMLTMIFSAILRATGHVKLPMMTSIVAVCINIVLNYLL 180 +D+ +I+ G +L I SYI ++ +S ILR G VK+PM+ S A+ IN LN+ L Sbjct: 124 QDKNVISLGANYLRIICFSYIVTGISFAYSIILRTVGQVKIPMIVSGTALAINTFLNFSL 183 Query: 181 IFGNFGFPEWGIKGAATSTLIARLFECCFIIAAAYKLRLPGVTHIRDWFGAPTALLSKFI 240 ++G F G+ G+A +TLIAR E ++ A YKL+LP + + T + +F Sbjct: 184 VYGIGSFHGIGVYGSAIATLIARFIEFFLMLLAIYKLKLPIAAKLHELLDLSTTFVKRFF 243 Query: 241 RTTYPIVLTELIWVLGETVYAIIYSRMGTVEITAMTITYPLQXXXXXXXXXXXXXXXXMI 300 + P++L E +W LG T YA +Y+ MGT I A IT + MI Sbjct: 244 KVAVPVMLNESMWGLGVTAYAAVYAHMGTAVIAATNITSTIDRLSMVVFFGFGNAAAIMI 303 Query: 301 GNKLGADEQDAALDFSKRFIKLGIFVSLGIGVLIAAIAPLYVSIFQVSEEAHHMGTRLVW 360 GN++G ++ A ++KRFI L F+ + +G+++ +P + + ++ + H ++ Sbjct: 304 GNRIGQKDEKTAFLYAKRFIILSPFIGIFMGIILYTSSPAILYAYNINSQVHMYSKEILH 363 Query: 361 VFAGFLWVKVANMILAGGILNSGGDSKFVFAMEAAATWLIGVPSGLLMSFVWKQPVFLVY 420 + FLW+K+ N GIL SGGD+KF ++ WL+GVP L +K P+ +VY Sbjct: 364 IIGMFLWIKIFNYTNVVGILRSGGDTKFCLMLDMGGMWLVGVPFVALTGIYFKLPIGIVY 423 Query: 421 MILSLEEVVRLSIGLARIHSRKWMKNLVAEIS 452 + +EE V+L +GL R+ S+KW+ NLV++ S Sbjct: 424 YFVFMEEFVKLLVGLPRLLSKKWINNLVSKPS 455 >C4ICG8.1 C4ICG8_CLOBU Mate efflux family protein Length = 446 Score = 291 bits (744), Expect = 1e-76, Method: Compositional matrix adjust. Identities = 162/443 (36%), Positives = 254/443 (57%) Query: 5 SKADFYSKIYTIAIPVTLQSLIMALLTLTDQLMVGQLGDVAVASVGISIKIYGIITVVLA 64 +K FY I +IA+P+T+QS + +++ D++MVGQLG V++A G+ K + +V ++ Sbjct: 3 NKDKFYKDILSIAVPITVQSFFQSSVSVIDEIMVGQLGSVSIAGAGLGGKFSSLFSVTIS 62 Query: 65 GLATGVSIYAAQFWGKKDEKSTAQXXXXXXXXXXXISIIFTLPVHFFPQFCLGLFTKDQR 124 +ATG I +Q++G K++K IS+ FT+ FP + +++ D Sbjct: 63 AIATGAGILISQYYGSKNKKGINDSFICNMYLSLIISVFFTVISFVFPIKLMSIYSTDLD 122 Query: 125 LITEGDIFLEITSLSYIPVMLTMIFSAILRATGHVKLPMMTSIVAVCINIVLNYLLIFGN 184 I +L+I S+ +IP+ LT+I S LR G+ K PM SI++V IN LNYLLIFG Sbjct: 123 TINASASYLKIVSIGFIPMTLTLIISTFLRCIGYAKYPMCASIISVFINTGLNYLLIFGK 182 Query: 185 FGFPEWGIKGAATSTLIARLFECCFIIAAAYKLRLPGVTHIRDWFGAPTALLSKFIRTTY 244 FP G++GAA +T IAR+FE I ++ +I + K I Y Sbjct: 183 SIFPAMGLEGAAWATNIARIFEFILIFIFLLLIKRKKKLYISFNLRLDFNFIRKVILILY 242 Query: 245 PIVLTELIWVLGETVYAIIYSRMGTVEITAMTITYPLQXXXXXXXXXXXXXXXXMIGNKL 304 P+++ E +W LGE YA+IY R+GT AMT+T P+Q + G L Sbjct: 243 PMLICEFLWSLGENGYAMIYGRIGTEACAAMTLTGPIQSLLIGIFSGIASASGIISGKAL 302 Query: 305 GADEQDAALDFSKRFIKLGIFVSLGIGVLIAAIAPLYVSIFQVSEEAHHMGTRLVWVFAG 364 G + + A SK+ K+G+ S+ IG+ ++AIA LYV +FQV+ + H +++ F+ Sbjct: 303 GDRQFEDAYIMSKKIFKIGVVGSIIIGIFLSAIASLYVKMFQVNYDIKHNTIYILYGFSL 362 Query: 365 FLWVKVANMILAGGILNSGGDSKFVFAMEAAATWLIGVPSGLLMSFVWKQPVFLVYMILS 424 L+ KV+NMIL GGIL SGG++K V ++ TW+ G+P G + +F++K P++ VY +LS Sbjct: 363 ILFAKVSNMILGGGILRSGGNTKLVMFIDIIGTWMFGIPIGFIAAFIFKMPIYQVYFLLS 422 Query: 425 LEEVVRLSIGLARIHSRKWMKNL 447 LEE++RL I L R W++N+ Sbjct: 423 LEEIIRLFISLFVFKKRLWIRNI 445 >B1QUL4.1 B1QUL4_CLOBU Putative Na+ driven multidrug efflux pump Length = 446 Score = 291 bits (744), Expect = 1e-76, Method: Compositional matrix adjust. Identities = 162/443 (36%), Positives = 254/443 (57%) Query: 5 SKADFYSKIYTIAIPVTLQSLIMALLTLTDQLMVGQLGDVAVASVGISIKIYGIITVVLA 64 +K FY I +IA+P+T+QS + +++ D++MVGQLG V++A G+ K + +V ++ Sbjct: 3 NKDKFYKDILSIAVPITVQSFFQSSVSVIDEIMVGQLGSVSIAGAGLGGKFSSLFSVTIS 62 Query: 65 GLATGVSIYAAQFWGKKDEKSTAQXXXXXXXXXXXISIIFTLPVHFFPQFCLGLFTKDQR 124 +ATG I +Q++G K++K IS+ FT+ FP + +++ D Sbjct: 63 AIATGAGILISQYYGSKNKKGINDSFICNMYLSLIISVFFTVISFVFPIKLMSIYSTDLD 122 Query: 125 LITEGDIFLEITSLSYIPVMLTMIFSAILRATGHVKLPMMTSIVAVCINIVLNYLLIFGN 184 I +L+I S+ +IP+ LT+I S LR G+ K PM SI++V IN LNYLLIFG Sbjct: 123 TINASASYLKIVSIGFIPMTLTLIISTFLRCIGYAKYPMCASIISVFINTGLNYLLIFGK 182 Query: 185 FGFPEWGIKGAATSTLIARLFECCFIIAAAYKLRLPGVTHIRDWFGAPTALLSKFIRTTY 244 FP G++GAA +T IAR+FE I ++ +I + K I Y Sbjct: 183 SIFPAMGLEGAAWATNIARIFEFILIFIFLLLIKRKKKLYISFNLRLDFNFIRKVILILY 242 Query: 245 PIVLTELIWVLGETVYAIIYSRMGTVEITAMTITYPLQXXXXXXXXXXXXXXXXMIGNKL 304 P+++ E +W LGE YA+IY R+GT AMT+T P+Q + G L Sbjct: 243 PMLICEFLWSLGENGYAMIYGRIGTEACAAMTLTGPIQSLLIGIFSGIASASGIISGKAL 302 Query: 305 GADEQDAALDFSKRFIKLGIFVSLGIGVLIAAIAPLYVSIFQVSEEAHHMGTRLVWVFAG 364 G + + A SK+ K+G+ S+ IG+ ++AIA LYV +FQV+ + H +++ F+ Sbjct: 303 GDRQFEDAYIMSKKIFKIGVVGSIIIGIFLSAIASLYVKMFQVNYDIKHNTIYILYGFSL 362 Query: 365 FLWVKVANMILAGGILNSGGDSKFVFAMEAAATWLIGVPSGLLMSFVWKQPVFLVYMILS 424 L+ KV+NMIL GGIL SGG++K V ++ TW+ G+P G + +F++K P++ VY +LS Sbjct: 363 ILFAKVSNMILGGGILRSGGNTKLVMFIDIIGTWMFGIPIGFIAAFIFKMPIYQVYFLLS 422 Query: 425 LEEVVRLSIGLARIHSRKWMKNL 447 LEE++RL I L R W++N+ Sbjct: 423 LEEIIRLFISLFVFKKRLWIRNI 445 >A9BJ76.1 A9BJ76_PETMO MATE efflux family protein Length = 445 Score = 273 bits (698), Expect = 3e-71, Method: Compositional matrix adjust. Identities = 159/447 (35%), Positives = 242/447 (54%), Gaps = 8/447 (1%) Query: 8 DFYSKIYTIAIPVTLQSLIMALLTLTDQLMVGQLGDVAVASVGISIKIYGIITVVLAGLA 67 D Y +I IA P+ LQ +I + D LM+G LG+VA+ASVG+S + + + ++L GL Sbjct: 2 DIYKRIIRIAFPIALQQVIFTSVNFVDTLMIGLLGEVAIASVGLSNQFFFLYNLILFGLV 61 Query: 68 TGVSIYAAQFWGKKDEKSTAQXXXXXXXXXXXISIIFTLPVHFFPQFCLGLFTKDQRLIT 127 +G I+ AQ+WGKKDE ++ SI F L +F P+ L F+ D +I Sbjct: 62 SGGGIFFAQYWGKKDEDGLSRSVALTILSSLLFSIPFFLLSYFAPEMVLRFFSPDIEVIK 121 Query: 128 EGDIFLEITSLSYIPVMLTMIFSAILRATGHVKLPMMTSIVAVCINIVLNYLLIFGNFGF 187 G +L++ + SY +TM+FS +LR+ G LP++ +I+ + NIVLNYLLIFG GF Sbjct: 122 AGIPYLKVIAFSYPLFAITMVFSFMLRSIGKAHLPLIITIIELSTNIVLNYLLIFGKLGF 181 Query: 188 PEWGIKGAATSTLIARLFECCFIIAAAYKLRLPGVTHIRDWFGAPTALLSKFIRTTY--- 244 P GIKGAA TLIAR C + Y +LPG R +F L SKF++ + Sbjct: 182 PVLGIKGAAIGTLIARAIGCIATVFIVYVGKLPG----RAYFKHVAELNSKFVKNFFHYA 237 Query: 245 -PIVLTELIWVLGETVYAIIYSRMGTVEITAMTITYPLQXXXXXXXXXXXXXXXXMIGNK 303 P + E W LG T+Y+++Y+ MGT I A + ++ +IGN Sbjct: 238 LPTLANEFAWSLGFTMYSVVYAHMGTDVIAAQRVMSTIEGFAVSVTFSIANAASVIIGNI 297 Query: 304 LGADEQDAALDFSKRFIKLGIFVSLGIGVLIAAIAPLYVSIFQVSEEAHHMGTRLVWVFA 363 LG + + A + SK+ +KL +S+ ++ L V +F VSEE +M + + Sbjct: 298 LGVSKFEEAFETSKKALKLAELISVIAAIIGIVTTFLVVDVFDVSEEVKNMVKVTIVISM 357 Query: 364 GFLWVKVANMILAGGILNSGGDSKFVFAMEAAATWLIGVPSGLLMSFVWKQPVFLVYMIL 423 GFL VK+ N + G L +GGD++F F +EA+ WLIGVP + + VY + Sbjct: 358 GFLPVKIFNGMNVVGFLRAGGDTRFSFLVEASTLWLIGVPLAAVGGLILDLSFPTVYFLT 417 Query: 424 SLEEVVRLSIGLARIHSRKWMKNLVAE 450 +EE+++ I R S+KW++N++ E Sbjct: 418 MMEEIIKSIILFFRYRSKKWLRNVLEE 444 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.328 0.141 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,317,439,453 Number of extensions: 87724084 Number of successful extensions: 309852 Number of sequences better than 1.0e-05: 3407 Number of HSP's gapped: 305718 Number of HSP's successfully gapped: 3464 Length of query: 453 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 314 Effective length of database: 1,751,175,118 Effective search space: 549868987052 Effective search space used: 549868987052 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 133 (55.8 bits)