BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0346.0079.p (304 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q3ACI0.1 Q3ACI0_CARHZ Dihydrodipicolinate synthase EC=4.2.1.52 277 9e-73 A4W612.1 A4W612_ENT38 Dihydrodipicolinate synthetase 273 2e-71 B1XE11.1 B1XE11_ECODH CP4-6 prophage; predicted lyase/synthase 263 1e-68 P75682.1 YAGE_ECOLI Uncharacterized protein yagE 263 1e-68 A8ARR9.1 A8ARR9_CITK8 Putative uncharacterized protein 250 1e-64 >Q3ACI0.1 Q3ACI0_CARHZ Dihydrodipicolinate synthase EC=4.2.1.52 Length = 304 Score = 277 bits (708), Expect = 9e-73, Method: Compositional matrix adjust. Identities = 127/299 (42%), Positives = 198/299 (66%) Query: 3 IIHTNAGIIPPVPTILNEQQKFDRGGMQLLIDSLIDKGVHGLFFLGTAGEFNQFDAGERE 62 I++ G+IPP+ TI E FD G + LID LI+ GVHG+F LG++GEF+ +A ER+ Sbjct: 4 ILNKFRGVIPPMLTIFKEDGTFDWEGNKALIDFLINGGVHGIFVLGSSGEFSHLNAEERK 63 Query: 63 EIAEFCIDYTYGRLPVWIGTGSNTTSEALRLTRHAARSGATGVVVINPYYCKLSEESLFT 122 E A+F +DY GR+PV +GTGS+ T E + LT+HA GA GVVV++PYY LSE++L+ Sbjct: 64 EFAKFVVDYVAGRVPVLVGTGSSNTKEVVELTKHAKEIGADGVVVVSPYYWGLSEKNLYQ 123 Query: 123 HYAAIAEAAELPLMLYNFPALTGQDLSPAFVRRLAARYSQVVGIKETVDQLSHIRQMILL 182 H+A +A+ ++P++LYNFP LTGQ+LSP+ V +LA+ + +VGIK+T+D ++HIR++IL Sbjct: 124 HFATVAKVVDIPIVLYNFPDLTGQNLSPSLVAKLASDFPNIVGIKDTIDSIAHIRELILQ 183 Query: 183 VQEVNPAFAVFCGFDEYLLPTLXXXXXXXXXXXXXXXPQLMLGLFSSFQMNQFSEVLEYH 242 V+ V P F+V G+D++L TL P + + ++++F E + +H Sbjct: 184 VKTVRPDFSVLAGYDDHLFNTLAMGGDGVIPGTANFAPHIPVNIYNNFVKGNIEESMNWH 243 Query: 243 RKLIQIPPLYALDDPFIPAIKEAVRLAGVPVPAFSHSPANPWNDEKEQQLKQIFSNAGI 301 RKL+++P +Y+LD P I +KEA +L G+PV ++ PA ++ + LK + AG+ Sbjct: 244 RKLLRLPRIYSLDKPAISLLKEACKLCGLPVESYIRQPAGRADEAAVENLKTLLREAGL 302 >A4W612.1 A4W612_ENT38 Dihydrodipicolinate synthetase Length = 302 Score = 273 bits (697), Expect = 2e-71, Method: Compositional matrix adjust. Identities = 129/282 (45%), Positives = 181/282 (64%) Query: 8 AGIIPPVPTILNEQQKFDRGGMQLLIDSLIDKGVHGLFFLGTAGEFNQFDAGEREEIAEF 67 +GIIPPV TI + +FD+ G LID +I GV GLFFLG+ GEF+Q +A ER+ IA F Sbjct: 8 SGIIPPVSTIFSADGQFDKHGTAALIDDMISAGVDGLFFLGSGGEFSQLNAEERKAIARF 67 Query: 68 CIDYTYGRLPVWIGTGSNTTSEALRLTRHAARSGATGVVVINPYYCKLSEESLFTHYAAI 127 ID+ R+PV IGTG E + L++HA ++GA G+VVINPYY K+SE++L ++ + Sbjct: 68 TIDHVNRRVPVLIGTGGTNARETIELSQHAQQAGADGIVVINPYYWKVSEQNLIHYFQQV 127 Query: 128 AEAAELPLMLYNFPALTGQDLSPAFVRRLAARYSQVVGIKETVDQLSHIRQMILLVQEVN 187 A++ LP++LYNFPALTGQDLSP V+ LA +VGIK+T+D ++H+R MIL V+ + Sbjct: 128 ADSVTLPVILYNFPALTGQDLSPTLVKTLADSRRNIVGIKDTIDSIAHLRSMILTVKPAH 187 Query: 188 PAFAVFCGFDEYLLPTLXXXXXXXXXXXXXXXPQLMLGLFSSFQMNQFSEVLEYHRKLIQ 247 P F+V CG+D++L TL PQL + L +F+ + YH+ L+Q Sbjct: 188 PHFSVLCGYDDHLFNTLLLGGDGAISASGNFAPQLSVKLRQAFRDGDLDQAAHYHQTLLQ 247 Query: 248 IPPLYALDDPFIPAIKEAVRLAGVPVPAFSHSPANPWNDEKE 289 IP +Y LD PF+ IKEA+ L G PV PA P +D ++ Sbjct: 248 IPQIYQLDTPFVNVIKEAIVLCGRPVSTHVLPPAQPLDDARK 289 >B1XE11.1 B1XE11_ECODH CP4-6 prophage; predicted lyase/synthase Length = 309 Score = 263 bits (673), Expect = 1e-68, Method: Compositional matrix adjust. Identities = 126/281 (44%), Positives = 178/281 (63%) Query: 9 GIIPPVPTILNEQQKFDRGGMQLLIDSLIDKGVHGLFFLGTAGEFNQFDAGEREEIAEFC 68 GIIPPV TI + D+ G LID LI GV GLFFLG+ GEF+Q A ER+ IA F Sbjct: 16 GIIPPVSTIFTADGQLDKPGTAALIDDLIKAGVDGLFFLGSGGEFSQLGAEERKAIARFA 75 Query: 69 IDYTYGRLPVWIGTGSNTTSEALRLTRHAARSGATGVVVINPYYCKLSEESLFTHYAAIA 128 ID+ R+PV IGTG E + L++HA ++GA G+VVINPYY K+SE +L ++ +A Sbjct: 76 IDHVDRRVPVLIGTGGTNARETIELSQHAQQAGADGIVVINPYYWKVSEANLIRYFEQVA 135 Query: 129 EAAELPLMLYNFPALTGQDLSPAFVRRLAARYSQVVGIKETVDQLSHIRQMILLVQEVNP 188 ++ LP+MLYNFPALTGQDL+PA V+ LA S ++GIK+T+D ++H+R MI V+ +P Sbjct: 136 DSVTLPVMLYNFPALTGQDLTPALVKTLADSRSNIIGIKDTIDSVAHLRSMIHTVKGAHP 195 Query: 189 AFAVFCGFDEYLLPTLXXXXXXXXXXXXXXXPQLMLGLFSSFQMNQFSEVLEYHRKLIQI 248 F V CG+D++L TL PQ+ + L +++ ++ YH+ L+QI Sbjct: 196 HFTVLCGYDDHLFNTLLLGGDGAISASGNFAPQVSVNLLKAWRDGDVAKAAGYHQTLLQI 255 Query: 249 PPLYALDDPFIPAIKEAVRLAGVPVPAFSHSPANPWNDEKE 289 P +Y LD PF+ IKEA+ L G PV PA+P ++ ++ Sbjct: 256 PQMYQLDTPFVNVIKEAIVLCGRPVSTHVLPPASPLDEPRK 296 >P75682.1 YAGE_ECOLI Uncharacterized protein yagE Length = 309 Score = 263 bits (673), Expect = 1e-68, Method: Compositional matrix adjust. Identities = 126/281 (44%), Positives = 178/281 (63%) Query: 9 GIIPPVPTILNEQQKFDRGGMQLLIDSLIDKGVHGLFFLGTAGEFNQFDAGEREEIAEFC 68 GIIPPV TI + D+ G LID LI GV GLFFLG+ GEF+Q A ER+ IA F Sbjct: 16 GIIPPVSTIFTADGQLDKPGTAALIDDLIKAGVDGLFFLGSGGEFSQLGAEERKAIARFA 75 Query: 69 IDYTYGRLPVWIGTGSNTTSEALRLTRHAARSGATGVVVINPYYCKLSEESLFTHYAAIA 128 ID+ R+PV IGTG E + L++HA ++GA G+VVINPYY K+SE +L ++ +A Sbjct: 76 IDHVDRRVPVLIGTGGTNARETIELSQHAQQAGADGIVVINPYYWKVSEANLIRYFEQVA 135 Query: 129 EAAELPLMLYNFPALTGQDLSPAFVRRLAARYSQVVGIKETVDQLSHIRQMILLVQEVNP 188 ++ LP+MLYNFPALTGQDL+PA V+ LA S ++GIK+T+D ++H+R MI V+ +P Sbjct: 136 DSVTLPVMLYNFPALTGQDLTPALVKTLADSRSNIIGIKDTIDSVAHLRSMIHTVKGAHP 195 Query: 189 AFAVFCGFDEYLLPTLXXXXXXXXXXXXXXXPQLMLGLFSSFQMNQFSEVLEYHRKLIQI 248 F V CG+D++L TL PQ+ + L +++ ++ YH+ L+QI Sbjct: 196 HFTVLCGYDDHLFNTLLLGGDGAISASGNFAPQVSVNLLKAWRDGDVAKAAGYHQTLLQI 255 Query: 249 PPLYALDDPFIPAIKEAVRLAGVPVPAFSHSPANPWNDEKE 289 P +Y LD PF+ IKEA+ L G PV PA+P ++ ++ Sbjct: 256 PQMYQLDTPFVNVIKEAIVLCGRPVSTHVLPPASPLDEPRK 296 >A8ARR9.1 A8ARR9_CITK8 Putative uncharacterized protein Length = 323 Score = 250 bits (638), Expect = 1e-64, Method: Compositional matrix adjust. Identities = 121/289 (41%), Positives = 172/289 (59%) Query: 9 GIIPPVPTILNEQQKFDRGGMQLLIDSLIDKGVHGLFFLGTAGEFNQFDAGEREEIAEFC 68 GI PPVPTI++ + DR GM +ID +I+ G+ LG+ GEF+ F +R++IAEF Sbjct: 31 GIFPPVPTIVDNHGELDRAGMATMIDHVINNRADGMLILGSGGEFSHFSTEQRKQIAEFS 90 Query: 69 IDYTYGRLPVWIGTGSNTTSEALRLTRHAARSGATGVVVINPYYCKLSEESLFTHYAAIA 128 + Y GR+PV IG +T+E ++L HA +GATGV+V+NPYY KLS+++ FTHY IA Sbjct: 91 LQYVAGRVPVLIGIACASTAETIQLGEHAQNAGATGVLVVNPYYAKLSDDARFTHYKRIA 150 Query: 129 EAAELPLMLYNFPALTGQDLSPAFVRRLAARYSQVVGIKETVDQLSHIRQMILLVQEVNP 188 EA +P+ LYNFP LTGQD+ + RLA +VGIK+T+D +SH R++I V P Sbjct: 151 EALSIPVFLYNFPELTGQDIGLDVITRLAREVPNIVGIKDTIDNISHTREIINRVHPFRP 210 Query: 189 AFAVFCGFDEYLLPTLXXXXXXXXXXXXXXXPQLMLGLFSSFQMNQFSEVLEYHRKLIQI 248 F VF G+DEYLL TL P + G++ +F + ++L + Sbjct: 211 EFIVFSGYDEYLLDTLLLGGHGGIPATFNFAPHITRGIYQAFIREDLTTAKALQQQLATL 270 Query: 249 PPLYALDDPFIPAIKEAVRLAGVPVPAFSHSPANPWNDEKEQQLKQIFS 297 PLYAL+ PF IK A++L GV + PA P N+EK ++ I + Sbjct: 271 SPLYALEQPFFGVIKTAIKLTGVDISTAVVPPALPLNEEKTALVRNILA 319 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.323 0.139 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,734,027,692 Number of extensions: 69068444 Number of successful extensions: 157020 Number of sequences better than 1.0e-05: 3196 Number of HSP's gapped: 155721 Number of HSP's successfully gapped: 3196 Length of query: 304 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 169 Effective length of database: 1,788,859,178 Effective search space: 302317201082 Effective search space used: 302317201082 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)