BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0346.0058.p (408 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DBA5.1 B1DBA5_9BACL Germination protein, Ger(X)C family 461 e-128 B5V2B5.1 B5V2B5_BACCE Spore germination B3 GerAC family protein,... 368 e-100 B9IRC6.1 B9IRC6_BACCQ Germination response to the combination of... 363 2e-98 B7HWS2.1 B7HWS2_BACC7 Spore germination B3 GerAC family protein,... 363 2e-98 C3AW58.1 C3AW58_BACMY Spore germination protein KC 362 3e-98 >B1DBA5.1 B1DBA5_9BACL Germination protein, Ger(X)C family Length = 405 Score = 461 bits (1187), Expect = e-128, Method: Compositional matrix adjust. Identities = 219/390 (56%), Positives = 286/390 (73%), Gaps = 4/390 (1%) Query: 19 SGCWDSTEVNELAIELAWGIDKGQDGDVMISAQVIVXXXXXXXXXXXXXXXXXXXXFFVV 78 SGCWD++EVNE+A+EL WGIDK D VMISAQVI+ FFVV Sbjct: 20 SGCWDNSEVNEIAVELGWGIDKSPDKGVMISAQVIIPARIDGGQDGKVGGGKDKP-FFVV 78 Query: 79 TSSGMNTLDAVQQMQTKLSRRVFRGHRRVIIIGESLARHGIKDVLDTYTRDPNLKLRTDL 138 + GMNTL AVQQMQ KLSR VFRGHRRVI+IGES+AR GIKDV DTYTRDPNLKLRTD+ Sbjct: 79 SGDGMNTLAAVQQMQAKLSRLVFRGHRRVIVIGESMARRGIKDVFDTYTRDPNLKLRTDI 138 Query: 139 FVVKGSTAREFLNISYPLETIPGLGALGEYDQIGTLMEMGFINFLVSASSKGSSPTVPVL 198 F+VKG+TA EFL +YPLE I LG LGEYDQIGT +EMG +NFL +A+S+G+ P +P + Sbjct: 139 FIVKGATANEFLKTTYPLENISALGVLGEYDQIGTPVEMGLLNFLHAATSEGACPAIPAV 198 Query: 199 VSGLNSDLSQEGEDQDQSEKVDAEGFRIAGSGIFDQDLKLVGFLDVTEARALRWITGKLQ 258 G++S + +Q+ + EGF+I G+GIF++DLKLVGFL++ E RA+RW+T L Sbjct: 199 AIGMDS---KSDNQSNQNMSSNPEGFQITGTGIFNKDLKLVGFLNIEEGRAMRWVTANLN 255 Query: 259 KLTITTQIPDQTGNASMEVINPGSKIQPVIRGNQIKIFVTLTGKGDIRENNTRLDLTQTG 318 KLT+ IPD+ GN S+ + SKIQP ++ ++K VTLT G IRENNTRLDLT+T Sbjct: 256 KLTVPATIPDEEGNVSIILFKLHSKIQPTVQNGKLKFLVTLTANGAIRENNTRLDLTRTN 315 Query: 319 NVNLLQDTFNKHIEESVLRTITKVQKKYGTDVFGFGDDIHNKIPSQWKSLKNKWDQEFSK 378 N++L+Q+ ++ IEE+V RTI+KVQK+YG D+FGF D + K + WKS+ N W++EF + Sbjct: 316 NISLVQNALDRRIEETVSRTISKVQKEYGADIFGFSDTVKRKNLALWKSVSNDWEKEFRE 375 Query: 379 AEIFVKADLKVRRVGVTGPSVHLMDDEIKR 408 AE+ VKA++ VRR+GVTGP++ L DE+K+ Sbjct: 376 AEVSVKANITVRRIGVTGPALQLKSDEVKK 405 >B5V2B5.1 B5V2B5_BACCE Spore germination B3 GerAC family protein, putative Length = 395 Score = 368 bits (945), Expect = e-100, Method: Compositional matrix adjust. Identities = 182/408 (44%), Positives = 261/408 (63%), Gaps = 13/408 (3%) Query: 1 MNKKRIVSSCLLLSLIFTSGCWDSTEVNELAIELAWGIDKGQDGDVMISAQVIVXXXXXX 60 M+ + +S L++SL F +GCWD TE+N+LAIEL WG+D+ ++ V ISAQ I+ Sbjct: 1 MSLHKALSMILVVSLCFLTGCWDRTELNDLAIELGWGLDQAKNNKVEISAQFIIPSKMGM 60 Query: 61 XXXXXXXXXXXXXXFFVVTSSGMNTLDAVQQMQTKLSRRVFRGHRRVIIIGESLARHGIK 120 F+ + G +T +AVQ MQTK+SR +FRGH+RVI+IGE +A++G+ Sbjct: 61 GQSGRSNTGKS---VFIESGIGKDTHEAVQMMQTKMSREIFRGHQRVILIGEKMAKNGLA 117 Query: 121 DVLDTYTRDPNLKLRTDLFVVKGSTAREFLNISYPLETIPGLGALGEYDQIGTLMEMGFI 180 VLD Y+RDP+++LR D FV+KG TA+EFL S PLE+IP LGAL E+ QI L +M + Sbjct: 118 SVLDAYSRDPDIRLRADAFVIKGHTAKEFLKASIPLESIPALGALKEHMQIEALGDMSLL 177 Query: 181 NFLVSASSKGSSPTVPVLVSGLNSDLSQEGEDQDQSEKVDAEGFRIAGSGIFDQDLKLVG 240 +FL++++S G +P +PV+ LN + K + +GF+I G IF+ D KLVG Sbjct: 178 HFLIASTSDGITPILPVI--KLNVS--------HKKGKTEVKGFQIVGGAIFNNDFKLVG 227 Query: 241 FLDVTEARALRWITGKLQKLTITTQIPDQTGNASMEVINPGSKIQPVIRGNQIKIFVTLT 300 +L++ E WI +L K T+T G+AS+ + KI P I+GN IK + + Sbjct: 228 YLNMQEWLTTLWIVNRLSKQTVTASASKGNGSASLYMTKINRKIIPTIQGNTIKCDIVFS 287 Query: 301 GKGDIRENNTRLDLTQTGNVNLLQDTFNKHIEESVLRTITKVQKKYGTDVFGFGDDIHNK 360 G+G I+ENNT LDLTQ N+ LL+ K E+ L+TI KVQK+YGTD+FGFG+ IH K Sbjct: 288 GEGTIQENNTNLDLTQPKNITLLEHVLEKQSEKQALQTIKKVQKQYGTDIFGFGEAIHRK 347 Query: 361 IPSQWKSLKNKWDQEFSKAEIFVKADLKVRRVGVTGPSVHLMDDEIKR 408 PS+WK LK W ++F KA++ V L +RRVG+TGP + L +E+K+ Sbjct: 348 YPSEWKGLKKNWSKQFRKAQVSVHTKLTIRRVGLTGPPLQLKKNEMKK 395 >B9IRC6.1 B9IRC6_BACCQ Germination response to the combination of glucose, fructose, L-asparagine, and KCl Length = 395 Score = 363 bits (931), Expect = 2e-98, Method: Compositional matrix adjust. Identities = 176/408 (43%), Positives = 260/408 (63%), Gaps = 13/408 (3%) Query: 1 MNKKRIVSSCLLLSLIFTSGCWDSTEVNELAIELAWGIDKGQDGDVMISAQVIVXXXXXX 60 M+ + +S L++SL F +GCWD TE+N+LAIEL WG+D+ ++ V ISAQ I+ Sbjct: 1 MSLHKALSMILVVSLCFLTGCWDRTELNDLAIELGWGLDQAKNNKVEISAQFIIPSKMGM 60 Query: 61 XXXXXXXXXXXXXXFFVVTSSGMNTLDAVQQMQTKLSRRVFRGHRRVIIIGESLARHGIK 120 F+ + G +T +AVQ MQTK+SR +FRGH+RVI+IGE +A++G+ Sbjct: 61 GQSGRSNAGKS---VFIESGIGKDTHEAVQMMQTKMSREIFRGHQRVILIGEKMAKNGLA 117 Query: 121 DVLDTYTRDPNLKLRTDLFVVKGSTAREFLNISYPLETIPGLGALGEYDQIGTLMEMGFI 180 VLD Y+RDP+++LR D FV+KG TA+EFL S PLE+IP LGAL E+ QI L +M + Sbjct: 118 SVLDAYSRDPDIRLRADAFVIKGHTAKEFLKASIPLESIPALGALKEHMQIEALGDMSLL 177 Query: 181 NFLVSASSKGSSPTVPVLVSGLNSDLSQEGEDQDQSEKVDAEGFRIAGSGIFDQDLKLVG 240 +FL++++S G +P +PV+ ++ + K + +GF+I G IF+ D KL+G Sbjct: 178 HFLIASTSDGITPILPVIKLNVS----------HKKGKTEVKGFQIVGGAIFNNDFKLIG 227 Query: 241 FLDVTEARALRWITGKLQKLTITTQIPDQTGNASMEVINPGSKIQPVIRGNQIKIFVTLT 300 +L++ E WI +L K T+T G+AS+ + KI P I+G+ IK + + Sbjct: 228 YLNMQEWLTTLWIVNRLSKQTVTASASKGNGSASLYMTKINRKIIPTIQGDTIKCDIVFS 287 Query: 301 GKGDIRENNTRLDLTQTGNVNLLQDTFNKHIEESVLRTITKVQKKYGTDVFGFGDDIHNK 360 G+G I+ENNT LDL+Q N+ LL+ K E+ L+TI KVQK+YGTD+FGFG+ IH K Sbjct: 288 GEGTIQENNTNLDLSQPKNITLLEHVLEKQSEKQALQTIKKVQKQYGTDIFGFGEAIHRK 347 Query: 361 IPSQWKSLKNKWDQEFSKAEIFVKADLKVRRVGVTGPSVHLMDDEIKR 408 PS+WK LK W ++F K ++ V L +RRVG+TGP + L +E+K+ Sbjct: 348 YPSEWKGLKKNWSKQFRKVQVSVHTKLTIRRVGLTGPPLQLKKNEMKK 395 >B7HWS2.1 B7HWS2_BACC7 Spore germination B3 GerAC family protein, putative Length = 395 Score = 363 bits (931), Expect = 2e-98, Method: Compositional matrix adjust. Identities = 176/408 (43%), Positives = 260/408 (63%), Gaps = 13/408 (3%) Query: 1 MNKKRIVSSCLLLSLIFTSGCWDSTEVNELAIELAWGIDKGQDGDVMISAQVIVXXXXXX 60 M+ + +S L++SL F +GCWD TE+N+LAIEL WG+D+ ++ V ISAQ I+ Sbjct: 1 MSLHKALSMILVVSLCFLTGCWDRTELNDLAIELGWGLDQAKNNKVEISAQFIIPSKMGM 60 Query: 61 XXXXXXXXXXXXXXFFVVTSSGMNTLDAVQQMQTKLSRRVFRGHRRVIIIGESLARHGIK 120 F+ + G +T +AVQ MQTK+SR +FRGH+RVI+IGE +A++G+ Sbjct: 61 GQSGRSNAGKS---VFIESGIGKDTHEAVQMMQTKMSREIFRGHQRVILIGEKMAKNGLA 117 Query: 121 DVLDTYTRDPNLKLRTDLFVVKGSTAREFLNISYPLETIPGLGALGEYDQIGTLMEMGFI 180 VLD Y+RDP+++LR D FV+KG TA+EFL S PLE+IP LGAL E+ QI L +M + Sbjct: 118 SVLDAYSRDPDIRLRADAFVIKGHTAKEFLKASIPLESIPALGALKEHMQIEALGDMSLL 177 Query: 181 NFLVSASSKGSSPTVPVLVSGLNSDLSQEGEDQDQSEKVDAEGFRIAGSGIFDQDLKLVG 240 +FL++++S G +P +PV+ ++ + K + +GF+I G IF+ D KL+G Sbjct: 178 HFLIASTSDGITPILPVIKLNVS----------HKKGKTEVKGFQIVGGAIFNNDFKLIG 227 Query: 241 FLDVTEARALRWITGKLQKLTITTQIPDQTGNASMEVINPGSKIQPVIRGNQIKIFVTLT 300 +L++ E WI +L K T+T G+AS+ + KI P I+G+ IK + + Sbjct: 228 YLNMQEWLTTLWIVNRLSKQTVTASASKGNGSASLYMTKINRKIIPTIQGDTIKCDIVFS 287 Query: 301 GKGDIRENNTRLDLTQTGNVNLLQDTFNKHIEESVLRTITKVQKKYGTDVFGFGDDIHNK 360 G+G I+ENNT LDL+Q N+ LL+ K E+ L+TI KVQK+YGTD+FGFG+ IH K Sbjct: 288 GEGTIQENNTNLDLSQPKNITLLEHVLEKQSEKQALQTIKKVQKQYGTDIFGFGEAIHRK 347 Query: 361 IPSQWKSLKNKWDQEFSKAEIFVKADLKVRRVGVTGPSVHLMDDEIKR 408 PS+WK LK W ++F K ++ V L +RRVG+TGP + L +E+K+ Sbjct: 348 YPSEWKGLKKNWSKQFRKVQVSVHTKLTIRRVGLTGPPLQLKKNEMKK 395 >C3AW58.1 C3AW58_BACMY Spore germination protein KC Length = 387 Score = 362 bits (930), Expect = 3e-98, Method: Compositional matrix adjust. Identities = 180/398 (45%), Positives = 257/398 (64%), Gaps = 13/398 (3%) Query: 11 LLLSLIFTSGCWDSTEVNELAIELAWGIDKGQDGDVMISAQVIVXXXXXXXXXXXXXXXX 70 L++SL F +GCWD TE+N+LAIEL WG+D+ ++ V ISAQ I+ Sbjct: 3 LVVSLFFLTGCWDRTELNDLAIELGWGLDQAKNNKVEISAQFIIPSKMGMGQSGRSNAGK 62 Query: 71 XXXXFFVVTSSGMNTLDAVQQMQTKLSRRVFRGHRRVIIIGESLARHGIKDVLDTYTRDP 130 F+ + +G +T +AVQ MQTK+SR +FRGH+RVI+IGE +A++G+ VLD Y+RDP Sbjct: 63 A---VFIESGTGRDTHEAVQMMQTKMSREIFRGHQRVILIGEKMAKNGLTPVLDAYSRDP 119 Query: 131 NLKLRTDLFVVKGSTAREFLNISYPLETIPGLGALGEYDQIGTLMEMGFINFLVSASSKG 190 +++LR D FV+KG+TA+EFL S PLE IP LGAL E+ QI TL + +NFL++ +S G Sbjct: 120 DIRLRADAFVIKGNTAKEFLKASIPLEAIPALGALKEHMQIETLGDTSLLNFLIAGTSDG 179 Query: 191 SSPTVPVLVSGLNSDLSQEGEDQDQSEKVDAEGFRIAGSGIFDQDLKLVGFLDVTEARAL 250 +P +PV+ LN+ ++ K + +GF+I G IF+ D KLVG+L++ E Sbjct: 180 ITPILPVI--KLNTS--------NKKGKTEVKGFQIVGGAIFNNDFKLVGYLNMQEWLTT 229 Query: 251 RWITGKLQKLTITTQIPDQTGNASMEVINPGSKIQPVIRGNQIKIFVTLTGKGDIRENNT 310 WI L K TIT + G+AS+ + KI P I+G+ IK V +G+G I+ENNT Sbjct: 230 LWIVNHLSKQTITATVSKGNGSASLYMTKIKRKIIPTIQGDTIKCDVVFSGEGTIQENNT 289 Query: 311 RLDLTQTGNVNLLQDTFNKHIEESVLRTITKVQKKYGTDVFGFGDDIHNKIPSQWKSLKN 370 LDL+Q N+ L++ K E+ L+TI KVQK+Y TD+FGFG+ IH K PS+WK LK Sbjct: 290 NLDLSQPKNLTLVEHVLEKQSEKQALQTIKKVQKQYRTDIFGFGEAIHQKYPSEWKGLKK 349 Query: 371 KWDQEFSKAEIFVKADLKVRRVGVTGPSVHLMDDEIKR 408 W ++F K ++ V L +RRVG+TGPS+ L +EIK+ Sbjct: 350 NWSKQFRKVQVSVHTKLTIRRVGLTGPSLQLKKNEIKK 387 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.136 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,214,288,925 Number of extensions: 88588297 Number of successful extensions: 218958 Number of sequences better than 1.0e-05: 335 Number of HSP's gapped: 218476 Number of HSP's successfully gapped: 338 Length of query: 408 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 270 Effective length of database: 1,760,596,133 Effective search space: 475360955910 Effective search space used: 475360955910 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)