BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0346.0057.p (361 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DBA6.1 B1DBA6_9BACL Spore germination protein 427 e-117 A7GUP4.1 A7GUP4_BACCN Spore germination protein 162 6e-38 Q4V1W2.1 Q4V1W2_BACCZ Spore germination protein KB 156 2e-36 B7GJD3.1 B7GJD3_ANOFW Spore germination protein KB 150 2e-34 A3F904.1 A3F904_BACME GerVB 143 2e-32 >B1DBA6.1 B1DBA6_9BACL Spore germination protein Length = 362 Score = 427 bits (1097), Expect = e-117, Method: Compositional matrix adjust. Identities = 209/357 (58%), Positives = 253/357 (70%) Query: 2 QKISAYQLXXXXXXXXXXXXXXXXXXXXXXREAWIAELISCSLGVILILIYTALMRMNPG 61 KI+ YQL ++AWI L+S LGV ++L+YT LMR+NPG Sbjct: 2 NKITVYQLFAITFIFQIGTTIIFGFGGQAGKDAWIGVLLSSFLGVFVVLVYTVLMRLNPG 61 Query: 62 LSLVEWFPAQLGRWIGTPIAFLYPLLFLYTVGRVVADIRDMVSXXXXXXXXXXXXXGLFV 121 L+LV+WFPAQ GRWIGTPIAFLYPLLF+Y GR+ ADIRDMVS G+FV Sbjct: 62 LTLVQWFPAQFGRWIGTPIAFLYPLLFIYIAGRITADIRDMVSTTLLFNTPLIIISGVFV 121 Query: 122 IIIAYCVYGGALIIARLGEIFXXXXXXXXXXXXXXXXXXGVMHIRNIQPVMEHGFGPIWN 181 IIAYCVYGG ++RLGE+F VMH N+ PV+E G+GP+W Sbjct: 122 FIIAYCVYGGITRVSRLGEMFLPIVLLLFSIEVILLISSNVMHYHNLLPVLEKGWGPVWK 181 Query: 182 VVYPAGITQPFGETLALAMIWPLSKTPSKVMKITVLSTLLSGIMIACLDIFVISVFGGVF 241 VYP GITQ +GE++ LAM W +K P+KVMKIT+L+TL+SGIM+ D+ + VFG +F Sbjct: 182 TVYPPGITQSYGESIILAMFWTETKEPNKVMKITILTTLMSGIMVTIFDLMAVLVFGDMF 241 Query: 242 SRFLYPLYTLLSLISVGKFIENLQMFGVLYFLMTALLKGAIYMYAALKGVQQLTKLKSYH 301 S FLYPLYTLLSLIS+GKFIENLQMFGVLY LMTALLKG I MYAA++G+QQLT +KSY Sbjct: 242 SGFLYPLYTLLSLISIGKFIENLQMFGVLYLLMTALLKGVIEMYAAVRGIQQLTNMKSYR 301 Query: 302 TLMIPGCIIAWLLGMTMSRNIAEHIYYHHFKILVPYVWVPLFIILPTVLLLVAWFRQ 358 L+IP C +A LGMTMS+NIAEHIY HHF+ILVPYVWVPLF+ILPT+LLLV W R+ Sbjct: 302 ALVIPSCAVALFLGMTMSKNIAEHIYRHHFEILVPYVWVPLFLILPTILLLVTWLRR 358 >A7GUP4.1 A7GUP4_BACCN Spore germination protein Length = 364 Score = 162 bits (409), Expect = 6e-38, Method: Compositional matrix adjust. Identities = 96/328 (29%), Positives = 168/328 (51%), Gaps = 2/328 (0%) Query: 32 REAWIAELISCSLGVILILIYTALMRMNPGLSLVEWFPAQLGRWIGTPIAFLYPLLFLYT 91 ++AW+ L+ G +L+ IYT L G +L + P +G+ IG P+ +Y F+Y Sbjct: 34 QDAWLVILVGMFCGFVLMWIYTKLFEYYSGDTLTQMIPKIVGKLIGYPLNVIYIFYFIYI 93 Query: 92 VGRVVADIRDMVSXXXXXXXXXXXXXGLFVIIIAYCVYGGALIIARLGEIFXXXXXXXXX 151 RV+ D ++++ G F+++I YC++GG + R+GEIF Sbjct: 94 AARVLRDFGELITGTFLPKTPLIIVIGSFMLVIVYCLHGGIEVFGRMGEIFFPFLFLVAI 153 Query: 152 XXXXXXXXXGVMHIRNIQPVMEHGFGPIWNVVYPAGITQPFGETLALAMIWPLSKTPSKV 211 V+ I + PV+E GFGP+W +P IT PFGE + M WP KV Sbjct: 154 LTWAIVYSSQVVDIEQLAPVLEKGFGPVWEAAFPLVITFPFGEMVLFMMFWPALHDSRKV 213 Query: 212 MKITVLSTLLSGIMIACLDIFVISVFGGVFSRF-LYPLYTLLSLISVGKFIENLQMFGVL 270 K+ ++ L++GI++ I ++SV G + YPL T++ + S+G IE + ++ Sbjct: 214 KKLGMVVVLVAGILLTVNTISILSVIGPNLVKMKTYPLLTIVRMASIGNLIERIDAAIII 273 Query: 271 YFLMTALLKGAIYMYAALKGVQQLTKLKSYHTLMIPGCIIAWLLGMTMSRNIAEHIYYHH 330 ++ K ++Y A G QL +LKSY ++++ + LL + ++ N EH Sbjct: 274 TMMIGGFFKVGSFLYGAAVGTAQLFRLKSYRSIVVVFGAVVVLLSIMIATNYMEHEEIGI 333 Query: 331 FKILVPYVWVPLFIILPTVLLLVAWFRQ 358 K+ + ++ +PL I++P +LL++A R+ Sbjct: 334 KKVPI-FLHIPLQIVIPVILLVIAMIRK 360 >Q4V1W2.1 Q4V1W2_BACCZ Spore germination protein KB Length = 363 Score = 156 bits (395), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 96/329 (29%), Positives = 171/329 (51%), Gaps = 4/329 (1%) Query: 32 REAWIAELISCSLGVILILIYTALMRMNPGLSLVEWFPAQLGRWIGTPIAFLYPLLFLYT 91 ++AW+A LI G++L+ IYT L G +L + P +G+++G P+ +Y L F+Y Sbjct: 33 QDAWVAILIGMFCGLVLMWIYTKLFEYYVGDTLTQMIPKIVGKFVGYPLIVIYILYFVYI 92 Query: 92 VGRVVADIRDMVSXXXXXXXXXXXXXGLFVIIIAYCVYGGALIIARLGEIFXXXXXXXXX 151 RV+ D ++++ G +++I YC+ GG + R+GE+F Sbjct: 93 AARVLRDFGELITGTFLPKTPLIIVMGCLMVVIVYCLSGGIEVFGRMGEVFFPFLFLIAV 152 Query: 152 XXXXXXXXXGVMHIRNIQPVMEHGFGPIWNVVYPAGITQPFGETLALAMIWPLSKTPSKV 211 ++ + + PV+E G G +W V+P IT PFGE + M WP + KV Sbjct: 153 VTWSIVYSSQIVDLERLTPVLEEGVGTVWKAVFPLTITFPFGELVLFMMFWPALQDSRKV 212 Query: 212 MKITVLSTLLSGIMIACLDIFVISVFGG--VFSRFLYPLYTLLSLISVGKFIENLQMFGV 269 K+ ++ L++GI++ + +ISV G + +R YPL T++ + S+G IE + + Sbjct: 213 KKLGLMVVLVAGILLTINMVNIISVIGPDWITTR-TYPLLTVVRMASIGNLIERIDAVVI 271 Query: 270 LYFLMTALLKGAIYMYAALKGVQQLTKLKSYHTLMIPGCIIAWLLGMTMSRNIAEHIYYH 329 + ++ K ++Y A G QL KLKSY +++I I LL + ++ EH Sbjct: 272 IAMIVGGFFKVGSFLYGAAIGTAQLFKLKSYRSMVIVFGTIIVLLSIMIATTYMEHEEIG 331 Query: 330 HFKILVPYVWVPLFIILPTVLLLVAWFRQ 358 K+ + ++ +PL I++P +LL++A R+ Sbjct: 332 LKKVPI-FLHIPLQIVIPVILLVIAIIRK 359 >B7GJD3.1 B7GJD3_ANOFW Spore germination protein KB Length = 370 Score = 150 bits (379), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 97/330 (29%), Positives = 161/330 (48%), Gaps = 2/330 (0%) Query: 32 REAWIAELISCSLGVILILIYTALMRMNPGLSLVEWFPAQLGRWIGTPIAFLYPLLFLYT 91 ++ W+A L LG L+ +Y L R P L L + +G + G + +Y L FLY Sbjct: 34 QDVWLAILCGMVLGGGLLFVYYRLFRYYPRLPLTSYSQRIVGSFFGRVLGLIYVLYFLYI 93 Query: 92 VGRVVADIRDMVSXXXXXXXXXXXXXGLFVIIIAYCVYGGALIIARLGEIFXXXXXXXXX 151 RV+ D +++ + ++ + Y VY G +IAR ++F Sbjct: 94 AARVLRDFGELLLAFAYSETPLFILNAVMMLTVMYAVYKGIEVIARASQLFFFSLYWILI 153 Query: 152 XXXXXXXXXGVMHIRNIQPVMEHGFGPIWNVVYPAGITQPFGETLALAMIWPLSKTPSKV 211 G + + +QP++E G+GPI++VV+ + PFGE + MI+P + KV Sbjct: 154 LGFVLIIITGAVDLHRLQPILEEGWGPIFHVVFTQTLYVPFGEMIVFTMIFPYLQKQEKV 213 Query: 212 MKITVLSTLLSGIMIACLDIFVISVFGG-VFSRFLYPLYTLLSLISVGKFIENLQMFGVL 270 MK L+ LSGI +A + I+V G + SR +PL + I V F+E L + ++ Sbjct: 214 MKAGFLALFLSGINLAIIMAMNIAVLGADIVSRSTFPLLNTIQEIRVANFLERLDVLFMI 273 Query: 271 YFLMTALLKGAIYMYAALKGVQQLTKLKSYHTLMIPGCIIAWLLGMTMSRNIAEHIYYHH 330 ++ K ++ YA + G L ++K Y L+ P I L + ++ N AEHI Sbjct: 274 ALIVGGFFKVGVFFYAGVIGAADLFQIKEYQRLVYPLGISVLFLSIAIASNFAEHI-KEG 332 Query: 331 FKILVPYVWVPLFIILPTVLLLVAWFRQFK 360 +I+ Y+ +PL I++P +LLL+A R K Sbjct: 333 LQIVTIYMHIPLQIVIPVLLLLIAMLRHQK 362 >A3F904.1 A3F904_BACME GerVB Length = 366 Score = 143 bits (361), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 104/360 (28%), Positives = 166/360 (46%), Gaps = 2/360 (0%) Query: 3 KISAYQLXXXXXXXXXXXXXXXXXXXXXXREAWIAELISCSLGVILILIYTALMRMNPGL 62 KISA QL ++AW+A LI +L L+Y L P L Sbjct: 5 KISASQLFILMVLFELGSSLLVPIAMDAKQDAWLAILIGMVGSFVLFLVYHKLHSYYPDL 64 Query: 63 SLVEWFPAQLGRWIGTPIAFLYPLLFLYTVGRVVADIRDMVSXXXXXXXXXXXXXGLFVI 122 E+ +G+ IGT +AF+Y L F+Y RV+ D M+ L ++ Sbjct: 65 LPTEYMQKIMGKGIGTVLAFVYILYFMYDAARVLRDFGAMLLTSAYPETPLFIAHTLLML 124 Query: 123 IIAYCVYGGALIIARLGEIFXXXXXXXXXXXXXXXXXXGVMHIRNIQPVMEHGFGPIWNV 182 ++ Y + G ++AR GEI G++ N+QPV+E G P+ V Sbjct: 125 VVIYTIRKGIEVVARSGEILFIFIYIFAIMGFVLIVCSGLIEFTNLQPVLEEGVLPVLKV 184 Query: 183 VYPAGITQPFGETLALAMIWPLSKTPSKVMKITVLSTLLSGIMIACLDIFVISVFG-GVF 241 + I PF E + MI P K K + +T LSGI + + ISV G + Sbjct: 185 AFTQTIYFPFAEAMVFTMILPYLKDQKKAKMTMLCATGLSGINLTITMLINISVLGVDLT 244 Query: 242 SRFLYPLYTLLSLISVGKFIENLQMFGVLYFLMTALLKGAIYMYAALKGVQQLTKLKSYH 301 SR +PL + + I V F+E L +F +L ++ +K ++ +YAA+ G L K+KS Sbjct: 245 SRSQFPLLSTVQSIQVADFLERLDVFFMLALVIGGFIKISVLLYAAVIGTANLFKIKSPS 304 Query: 302 TLMIPGCIIAWLLGMTMSRNIAEHIYYHHFKILVPYVWVPLFIILPTVLLLVAWFRQFKK 361 L P + + T++ N EH+ + K+ + + +P+ I+P++LLLVA+ + KK Sbjct: 305 RLSYPLGFVILFMASTIASNFQEHL-HEGLKVEMFILHMPILAIIPSLLLLVAFLKNRKK 363 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.332 0.147 0.464 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,725,414,700 Number of extensions: 60905996 Number of successful extensions: 209528 Number of sequences better than 1.0e-05: 179 Number of HSP's gapped: 209432 Number of HSP's successfully gapped: 179 Length of query: 361 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 224 Effective length of database: 1,770,017,148 Effective search space: 396483841152 Effective search space used: 396483841152 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (22.0 bits) S2: 132 (55.5 bits)