BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0346.0048.p (449 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K643.1 B3K643_9BACI Extracellular solute-binding protein family 1 372 e-101 B1D783.1 B1D783_9BACL Extracellular solute-binding protein family 1 352 3e-95 A4U8T8.1 A4U8T8_9BACT Sugar permease 266 3e-69 Q5WIQ4.1 Q5WIQ4_BACSK Sugar ABC transporter substrate-binding pr... 241 1e-61 B1DGI2.1 B1DGI2_9BACL Sugar ABC transporter substrate-binding pr... 216 3e-54 >B3K643.1 B3K643_9BACI Extracellular solute-binding protein family 1 Length = 468 Score = 372 bits (955), Expect = e-101, Method: Compositional matrix adjust. Identities = 192/427 (44%), Positives = 273/427 (63%), Gaps = 20/427 (4%) Query: 29 GCSSSEGKNGADSGNAAKDQGNAEQSGKPVTLKFTFWGSPQEKAAIEKATQAFTEKYPNI 88 G ++ G+NG + NA D +G+ VTLKFTFWGSP+EK A+E A +AF EK PNI Sbjct: 31 GSETNAGQNGGE--NAGTD------NGEKVTLKFTFWGSPEEKKAVESAIKAFEEKNPNI 82 Query: 89 KVDAIHIPEADYDAKITAMVAGNEAPDLGYMHGELADPWQ----KDGKFVNMFELLDADK 144 +D +HIP D+ K+ AM+AGNEAPD+ Y + W+ ++G N F+L+ D Sbjct: 83 TIDPVHIPGTDFLQKLNAMIAGNEAPDISY-----SAAWKLKMGEEGLIYNFFDLMKDDP 137 Query: 145 ELKRSDFLDYIWFKSSEDFAYGISTAGETFGLFYNKDLFDAAKVEYPPAKPEMAWSWDEF 204 +K+ D+L+Y W+ +D + G A L YN D+F A V PP K E AW+W++F Sbjct: 138 SIKKEDYLEYAWWNWEQDKSAGPYQASVVPSLMYNADMFQEAGVALPPTKAEEAWTWEQF 197 Query: 205 VKVAQQLTLDKNGKNAADPGFDPDNIKQFGVSFETWHGPVHATVINNEGEWVTKDGK-FG 263 V A++LTLD+NGK+ DPGFDP NIKQ+GV V++N G++++ DGK FG Sbjct: 198 VDAAKKLTLDRNGKHPDDPGFDPKNIKQYGVKLNLGWSSYMPMVLSNGGDYLSADGKEFG 257 Query: 264 FSQPEAAEAIQRLADLINVYHVAPSPVQMKSIPSQAVALQSKLAAMSMHGQWINLDLGAA 323 S+PEA EA+QR+ADLINV+HV+PSPVQ SIP+ A ALQSK AM + G W +LDL Sbjct: 258 LSKPEATEALQRIADLINVHHVSPSPVQASSIPAPATALQSKKVAMIIDGSWNHLDLSKT 317 Query: 324 KVNYDIGVLPKMKRSVPMGISGASVIYKSSKHQEEAWLLTKFLENPEGAIDLYKGGLWMP 383 +N+ +GVLP +K + G+ +I+KSSKH +E W +KFL +P+ +DL++ GLWMP Sbjct: 318 NINWGVGVLPVIKDYKTFFLGGSLIIFKSSKHPKETWEFSKFLTDPQNVLDLHQ-GLWMP 376 Query: 384 TMTKWYTDPELVAKWA-ENNPAHPSGFKDAMMNNLLNNGVPNGSYYIKNFSKVNAITFSG 442 + KWY DPEL+ +WA E P P+GF+DA+M + + P+ +KNF +++A + Sbjct: 377 QLNKWYEDPELIEEWANEELPGRPAGFQDAVMRSTYEHAEPSPENNVKNFVEIDAAVAAA 436 Query: 443 MDXXXXG 449 +D G Sbjct: 437 LDQVWLG 443 >B1D783.1 B1D783_9BACL Extracellular solute-binding protein family 1 Length = 483 Score = 352 bits (904), Expect = 3e-95, Method: Compositional matrix adjust. Identities = 179/410 (43%), Positives = 255/410 (62%), Gaps = 11/410 (2%) Query: 37 NGADSGNAAKDQGNAEQSGKPVTLKFTFWGSPQEKAAIEKATQAFTEKYPNIKVDAIHIP 96 +G DSG SG+ +TLK T WG+P E AA + A F + +PN+KVD I Sbjct: 52 SGTDSGTT---------SGEKITLKLTGWGAPAEVAAYKSAIANFEKTHPNVKVDFQAIS 102 Query: 97 EADYDAKITAMVAGNEAPDLGYMH-GELADPWQKDGKFVNMFELLDADKELKRSDFLDYI 155 DYD K+T MVAGN+ PD+ M +A P ++GKF+N+ + L D+++ + I Sbjct: 103 SGDYDTKLTTMVAGNDEPDVAMMESATIAFPLAEEGKFLNLQDFLAKDQQITLDSLVPNI 162 Query: 156 WFKSSEDFAYGISTAGETFGLFYNKDLFDAAKVEYPPAKPEMAWSWDEFVKVAQQLTLDK 215 + + GI ETFGLFYN+D+F A +E PP+ AW+WD+FV VA++LTLD Sbjct: 163 TYSQAPGNIIGIGPGPETFGLFYNEDVFKDAGIEPPPSDVSKAWNWDQFVDVAKKLTLDN 222 Query: 216 NGKNAADPGFDPDNIKQFGVSFETWHGPVHATVINNEGEWVTKDGK-FGFSQPEAAEAIQ 274 G+NA DP FDP NIKQFG++ TW G + +N G++++ DGK F +QPEA E Q Sbjct: 223 KGRNATDPSFDPKNIKQFGINLPTWWGAYSNFIYSNGGDFLSADGKTFALNQPEAVEVFQ 282 Query: 275 RLADLINVYHVAPSPVQMKSIPSQAVALQSKLAAMSMHGQWINLDLGAAKVNYDIGVLPK 334 ++ADLINVYHVAPSP+Q K+IP VALQ+K AM++ GQW + L +K NY++GVLP Sbjct: 283 KMADLINVYHVAPSPLQSKNIPGTNVALQTKKVAMAIDGQWASSSLAESKFNYNVGVLPV 342 Query: 335 MKRSVPMGISGASVIYKSSKHQEEAWLLTKFLENPEGAIDLYKGGLWMPTMTKWYTDPEL 394 MK V ++G I+KS+KH +E+W L K L +PE ++ + G WMP WYTD E Sbjct: 343 MKEPVTTVVAGMFSIFKSTKHPQESWELLKALLDPEASLGMLTNGTWMPAPKDWYTDSEK 402 Query: 395 VAKWAENNPAHPSGFKDAMMNNLLNNGVPNGSYYIKNFSKVNAITFSGMD 444 + KW ++ A PSG+ A+++ LLN+ + Y+KNF+K+ + +D Sbjct: 403 LDKWTKDLAARPSGYNGAVIDMLLNHSHATPTAYVKNFNKIMDVVNPALD 452 >A4U8T8.1 A4U8T8_9BACT Sugar permease Length = 450 Score = 266 bits (680), Expect = 3e-69, Method: Compositional matrix adjust. Identities = 145/383 (37%), Positives = 217/383 (56%), Gaps = 9/383 (2%) Query: 58 VTLKFTFWGSPQEKAAIEKATQAFTEKYPNIKVDAIHIPEADYDAKITAMVAGNEAPDLG 117 V L FTFWG E+ A+ + + F +PNI V HIP A Y+ KI+ M AG PD G Sbjct: 34 VELTFTFWGGTFERDAVNQTLETFNSLHPNIHVTGQHIPNAGYNEKISTMAAGGTLPDAG 93 Query: 118 YMHGELADPWQKDGKFVNMFELLDADKELKRSDFLDYIWFK-SSEDFAYGISTAGETFGL 176 YM +A W DG ++ +D +D L+ +W++ + G + A E+ L Sbjct: 94 YMDSSMALRWATDGLIKDLTTQFASDP--MAADRLENMWYRWDNGTKTIGTAAAVESMIL 151 Query: 177 FYNKDLFDAAKVEYPPAKPEMAWSWDEFVKVAQQLTLDKNGKNAADPGFDPDNIKQFGVS 236 +YN+DLFDAA ++YPP AW+WDEFV +A+QLT+D+ G NAA P FDPDNI FG++ Sbjct: 152 YYNRDLFDAAGIDYPPEFASEAWTWDEFVDIAKQLTVDRQGNNAASPDFDPDNIDVFGIA 211 Query: 237 FETWHGPVHATVINNEGEWVTKDG-KFGFSQPEAAEAIQRLADLINVYHVAPSPVQMKSI 295 F TW V +N G+ + DG + ++PEA EA+Q L DLI V+HVAP+P Q +S+ Sbjct: 212 FPTWWAGWLPFVYSNGGQLASDDGTELLINRPEAVEALQALQDLIYVHHVAPTPAQSQSL 271 Query: 296 PSQAVALQSKLAAMSMHGQWINLDLGAAKVNYDIGVLPKMKRSVPMGISGASVIYKSSKH 355 P+ + +Q AMS+ G W L + N+ GVLP +R + G +VI + ++H Sbjct: 272 PAADIMMQLGKVAMSVDGHWRTLAMSQLGFNWSFGVLPMHQRPATLIWGGVTVILEGTEH 331 Query: 356 QEEAWLLTKFLENPEGAIDLYKGGLWMPTMTKWYTDPELVAKWAENNP-AHPSGFKDAMM 414 +EA+ L FL +P + L+ GLWMP +YTD A+W + P +P + ++ Sbjct: 332 PDEAFELINFLSDPTQSA-LFTRGLWMPLQRIYYTDEAKAAEWLDAIPGVYPPESRGVLL 390 Query: 415 NNLLNNGVPNGS--YYIKNFSKV 435 + LN+ P +++KN ++ Sbjct: 391 DYTLNH-TPRQPPVFWLKNLDQI 412 >Q5WIQ4.1 Q5WIQ4_BACSK Sugar ABC transporter substrate-binding protein Length = 452 Score = 241 bits (615), Expect = 1e-61, Method: Compositional matrix adjust. Identities = 137/400 (34%), Positives = 217/400 (54%), Gaps = 16/400 (4%) Query: 54 SGKPVTLKFTFWGSPQEKAAIEKATQAFTEKYPNIKVDAIHIPEADYDAKITAMVAGNEA 113 + PV L+FT+WG P E+ A+E F ++P I+V A +P Y K+ M A Sbjct: 30 TNDPVELRFTYWGGPLEREAVETLISTFNNEHPGIQVRAQLVPINSYVEKMNVMAASKTL 89 Query: 114 PDLGYMHGELADPWQKDGKFVNMFELLDADKELKRSDFLDYIWFKSSEDFAYGISTAGET 173 PD+GY PW ++G ++ LL ++ ++ LDY ++ + G S A E Sbjct: 90 PDVGYFPEGSLYPWVENGMLRDLTPLLTGNEANEK---LDYTLYQFHDGPIAGGSVANEV 146 Query: 174 FGLFYNKDLFDAAKVEYPPAKPEMAWSWDEFVKVAQQLTLDKNGKNAADPGFDPDNIKQF 233 ++YNK LF A VE PP K E AWSWDEFV+VA++LT+D+NG+ A +PGFD + I+ + Sbjct: 147 INIYYNKQLFAEAGVELPPTKAEEAWSWDEFVEVAKRLTVDRNGRRANEPGFDENRIETY 206 Query: 234 GVS-FETWHGPVHATVINNEGEWVTKDGK-FGFSQPEAAEAIQRLADLINVYHVAPSPVQ 291 GVS F W + + + +N G +V + GK Q E+ E +Q +ADL+ V+HV P P Sbjct: 207 GVSNFTGW---IDSFLFSNGGGFVDETGKQLLIDQSESIEVLQHVADLMYVHHVMPKPSD 263 Query: 292 MKSIPSQAVALQSKLAAMSMHGQWINLDLGAAKV----NYDIGVLPKMKRSVPMGISGAS 347 + ++P+ AL +K AM++ GQW +LG A V ++ IGVLP ++ Sbjct: 264 LSTVPATDTALLTKRVAMAVDGQWAMQELGRASVEDGLDFGIGVLPYFEQPATTNYGTPI 323 Query: 348 VIYKSSK---HQEEAWLLTKFLENPEGAIDLYKGGLWMPTMTKWYTDPELVAKWAENNPA 404 V +++ K H++ ++ +PE + L GLWMP T WY D E + +W + P Sbjct: 324 VAFETGKEGEHRQAVDTFLTYIMDPENVLMLINEGLWMPNETAWYEDREKIERWI-DTPI 382 Query: 405 HPSGFKDAMMNNLLNNGVPNGSYYIKNFSKVNAITFSGMD 444 HP +++A+++ + V N +Y+ + K I +D Sbjct: 383 HPPEYEEAVIDWAKDYVVQNPNYFWDDRQKAGDIISPALD 422 >B1DGI2.1 B1DGI2_9BACL Sugar ABC transporter substrate-binding protein Length = 239 Score = 216 bits (551), Expect = 3e-54, Method: Compositional matrix adjust. Identities = 107/204 (52%), Positives = 143/204 (70%), Gaps = 2/204 (0%) Query: 248 VINNEGEWVTKDG-KFGFSQPEAAEAIQRLADLINVYHVAPSPVQMKSIPSQAVALQSKL 306 V++N G++V +DG +F + PEAAEAIQ+ ADLIN YHVAPSP Q K+IPS A AL S+ Sbjct: 10 VVSNGGQFVNQDGTQFKLADPEAAEAIQQWADLINKYHVAPSPTQSKNIPSGANALSSRK 69 Query: 307 AAMSMHGQWINLDLGAAK-VNYDIGVLPKMKRSVPMGISGASVIYKSSKHQEEAWLLTKF 365 AM++ GQW +DLGAAK +N+ IGV P MK M + VI+K +KH EEAW+L K+ Sbjct: 70 VAMTLDGQWSLVDLGAAKKLNFGIGVAPVMKNKATMTLGEPIVIFKDTKHPEEAWMLYKW 129 Query: 366 LENPEGAIDLYKGGLWMPTMTKWYTDPELVAKWAENNPAHPSGFKDAMMNNLLNNGVPNG 425 + NPE ++L GLWMP + KWY + +LVAKWA NPAHP G++DA+M N +NG PN Sbjct: 130 MMNPENTLELQTSGLWMPVLKKWYENADLVAKWAAGNPAHPEGYQDAVMRNAFDNGFPNT 189 Query: 426 SYYIKNFSKVNAITFSGMDXXXXG 449 SYY+KN SK+N++ ++ G Sbjct: 190 SYYVKNLSKINSMIDPALEQVWLG 213 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.314 0.132 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,854,329,988 Number of extensions: 125996361 Number of successful extensions: 231619 Number of sequences better than 1.0e-05: 591 Number of HSP's gapped: 231221 Number of HSP's successfully gapped: 591 Length of query: 449 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 310 Effective length of database: 1,751,175,118 Effective search space: 542864286580 Effective search space used: 542864286580 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 133 (55.8 bits)