BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0346.0041.p (284 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C0Z6I5.1 C0Z6I5_BREBN Putative uncharacterized protein 276 1e-72 B3KJ50.1 B3KJ50_9BACI Diguanylate cyclase 217 9e-55 B1D9K3.1 B1D9K3_9BACL Diguanylate cyclase 213 2e-53 B5S2K2.1 B5S2K2_RALSO Signal transduction protein ggdef domain 179 3e-43 Q8XY70.1 Q8XY70_RALSO Putative signal transduction ggdef domain ... 176 2e-42 >C0Z6I5.1 C0Z6I5_BREBN Putative uncharacterized protein Length = 530 Score = 276 bits (706), Expect = 1e-72, Method: Compositional matrix adjust. Identities = 138/258 (53%), Positives = 198/258 (76%) Query: 20 WGIVVVSPMALIHQQLIGHIQTIIAYSLIPFIILLSAVILIAHRLARPFAYLADLVSKMG 79 WG+VVVSP++++ +Q+ IQ+++ Y L F++L+ VI++A ++A+PF LA+LVSK G Sbjct: 272 WGVVVVSPISVVQEQVNRDIQSVLLYMLPSFLLLMLVVIVLARKIAKPFVSLANLVSKAG 331 Query: 80 KERIVLPPVKPHWSREADLLTKAVFLAVADIQKQTDQLTQEAATDTLTGLKNRRSLEYTL 139 ++ + L HW+REA+LL++A+ A+ +I+K+T+QLT +A TD LTGL NRR+LE T+ Sbjct: 332 RQEVELSEGSQHWNREAELLSRAIRFALTNIKKKTEQLTHDARTDPLTGLTNRRTLEETM 391 Query: 140 SQWIASGIPFSLIVLDVDRFKFVNDTYGHVTGDEVLKHVAGVIVSSLRPDDVCHRFGGEE 199 +W+A IPFS++++D+DRFK +NDTYGH GDEVL+H A +I SSLRP+DV RFGGEE Sbjct: 392 QKWMAEQIPFSIMMMDLDRFKAINDTYGHQAGDEVLRHFAKIISSSLRPEDVSCRFGGEE 451 Query: 200 FVILLARTRPAEAFNVAERVRRALEQRKGPIPTQVTVSQGIAHYPQHASEQAELLKKADQ 259 FV+L++ T EA+ VAER+RRALE R PI ++TVSQGIAHYP HA LL AD+ Sbjct: 452 FVVLVSHTGLEEAYQVAERIRRALETRTNPIGQRLTVSQGIAHYPSHAVSPEGLLHVADE 511 Query: 260 ALYRAKSNGRNRTVVAED 277 A+Y+AK GRN+T++AE+ Sbjct: 512 AMYKAKRAGRNQTMIAEE 529 >B3KJ50.1 B3KJ50_9BACI Diguanylate cyclase Length = 529 Score = 217 bits (553), Expect = 9e-55, Method: Compositional matrix adjust. Identities = 114/258 (44%), Positives = 154/258 (59%), Gaps = 1/258 (0%) Query: 19 GWGIVVVSPMALIHQQLIGHIQTIIAYSLIPFIILLSAVILIAHRLARPFAYLADLVSKM 78 GWGIV+ + ++ I + ++Q + L P ++L + A R+A PF L L + Sbjct: 268 GWGIVMQTSVSSILADVNRNLQDQLRSMLFPIALMLFIAVYTAARIAAPFTRLYKLTKSV 327 Query: 79 GKERIVLP-PVKPHWSREADLLTKAVFLAVADIQKQTDQLTQEAATDTLTGLKNRRSLEY 137 + V +PHW+REA L + + A + KQT L QEA TD LTGL NRR LE Sbjct: 328 AAGQTVSSQEFRPHWNREAGQLNRIMGAAAETMAKQTQSLQQEAITDPLTGLSNRRELER 387 Query: 138 TLSQWIASGIPFSLIVLDVDRFKFVNDTYGHVTGDEVLKHVAGVIVSSLRPDDVCHRFGG 197 L W A G P+++I+LD+D FK +NDTYGH TGD++LK VA V+V D VC RFGG Sbjct: 388 CLESWDALGEPYAVILLDIDHFKAINDTYGHQTGDQILKLVAAVMVHCAPADAVCSRFGG 447 Query: 198 EEFVILLARTRPAEAFNVAERVRRALEQRKGPIPTQVTVSQGIAHYPQHASEQAELLKKA 257 EEFVILL A+ +AER+RRA+ P +TVS G++ YP+H + ++ + A Sbjct: 448 EEFVILLRERSLDTAYRMAERIRRAILVSPTPYSGTLTVSIGVSLYPEHGGNREDVFQAA 507 Query: 258 DQALYRAKSNGRNRTVVA 275 D ALYRAK GRNRTV A Sbjct: 508 DMALYRAKGEGRNRTVAA 525 >B1D9K3.1 B1D9K3_9BACL Diguanylate cyclase Length = 533 Score = 213 bits (542), Expect = 2e-53, Method: Compositional matrix adjust. Identities = 119/260 (45%), Positives = 158/260 (60%), Gaps = 8/260 (3%) Query: 19 GWGIVVVSPMALIHQQLIGHIQTIIAYSLIPFIILLSAVIL---IAHRLARPFAYLADLV 75 GWGIV +P + +Q +I +S PF++LL VIL ++ +LA P LA Sbjct: 266 GWGIVSQTPQDHVTNISENVVQEMIKFS-APFVLLL--VILSFWLSRKLAHPLNRLATYA 322 Query: 76 SKMGKERIVLPPVKP--HWSREADLLTKAVFLAVADIQKQTDQLTQEAATDTLTGLKNRR 133 S++ L P++ W+ EA+ L V +A ++ Q ++L EA TD LTGL NRR Sbjct: 323 SRLSGGDEKLKPLQADVSWNYEANQLVDTVAVAFNKLRAQKEELHAEANTDALTGLTNRR 382 Query: 134 SLEYTLSQWIASGIPFSLIVLDVDRFKFVNDTYGHVTGDEVLKHVAGVIVSSLRPDDVCH 193 ++ + W G PFS+I++D+D FK VND +GH GDEVLK VAG++VS PDD C Sbjct: 383 TMGAITALWKQQGTPFSVIIMDLDHFKNVNDRHGHQMGDEVLKFVAGIMVSERGPDDYCC 442 Query: 194 RFGGEEFVILLARTRPAEAFNVAERVRRALEQRKGPIPTQVTVSQGIAHYPQHASEQAEL 253 RFGGEEF +LL AEA VAER+R +EQ PI VT+S G+A +P A E EL Sbjct: 443 RFGGEEFTMLLPHATEAEAVQVAERIRLRIEQSVTPIGESVTMSLGVATFPDAAEEVVEL 502 Query: 254 LKKADQALYRAKSNGRNRTV 273 K ADQALY+AK +GRNRTV Sbjct: 503 FKYADQALYQAKRDGRNRTV 522 >B5S2K2.1 B5S2K2_RALSO Signal transduction protein ggdef domain Length = 532 Score = 179 bits (453), Expect = 3e-43, Method: Compositional matrix adjust. Identities = 104/257 (40%), Positives = 142/257 (55%), Gaps = 1/257 (0%) Query: 19 GWGIVVVSPMALIHQQLIGHIQTIIAYSLIPFIILLSAVILIAHRLARPFAYLADLVSKM 78 GWGIVV P + L G + +++S+ F++ + + A ++RP LA+ + Sbjct: 253 GWGIVVQKPTETTLRDLNGLMLVTLSHSVPFFVLSIVFIWWFARSISRPLVELANEARLV 312 Query: 79 GK-ERIVLPPVKPHWSREADLLTKAVFLAVADIQKQTDQLTQEAATDTLTGLKNRRSLEY 137 ER+ W EA L +A+ + +L EA TD LTGL NRR + Sbjct: 313 ATPERLERIKTVSSWYIEAHRLKRALLRGTRLSSSRMARLNHEALTDPLTGLYNRRGVAD 372 Query: 138 TLSQWIASGIPFSLIVLDVDRFKFVNDTYGHVTGDEVLKHVAGVIVSSLRPDDVCHRFGG 197 L PFS++ LDVD FK +ND GH GD+VLKH+A +I R DD+ R GG Sbjct: 373 ALRLLAGRHQPFSVVTLDVDHFKRINDRDGHDVGDQVLKHIAALIREQARHDDITGRIGG 432 Query: 198 EEFVILLARTRPAEAFNVAERVRRALEQRKGPIPTQVTVSQGIAHYPQHASEQAELLKKA 257 EEF++LL P AF AER+RRA+E R P VTVS G+AHYP + E +LK + Sbjct: 433 EEFILLLPDATPEGAFKTAERLRRAVEARPNPTGQVVTVSLGMAHYPGASEEVDAVLKLS 492 Query: 258 DQALYRAKSNGRNRTVV 274 D ALY+AK +GRNRTV+ Sbjct: 493 DLALYQAKRSGRNRTVL 509 >Q8XY70.1 Q8XY70_RALSO Putative signal transduction ggdef domain transmembrane protein Length = 533 Score = 176 bits (446), Expect = 2e-42, Method: Compositional matrix adjust. Identities = 106/267 (39%), Positives = 142/267 (53%), Gaps = 5/267 (1%) Query: 19 GWGIVVVSPMALIHQQLIGHIQTIIAYSLIPFIILLSAVILIAHRLARPFAYLADLVSKM 78 GWGIV P L G + +++S+ F++ + + A ++RP LA + Sbjct: 253 GWGIVAQKPTETTLSDLNGLMLVTLSHSIPLFVLSIVFIWWFARSISRPLVELASQARLV 312 Query: 79 GK-ERIVLPPVKPHWSREADLLTKAVFLAVADIQKQTDQLTQEAATDTLTGLKNRRSLEY 137 ER+ W EA L +A+ + +L EA TD LTGL NRR + Sbjct: 313 ATPERLERIKTVSSWYIEAHRLKRALLRGTRLTSTRMARLNLEALTDPLTGLYNRRGVAN 372 Query: 138 TLSQWIASGIPFSLIVLDVDRFKFVNDTYGHVTGDEVLKHVAGVIVSSLRPDDVCHRFGG 197 L PFS + LDVD FK +ND +GH GD+VL+H+A +I R D+ R GG Sbjct: 373 ALRLHAGRHQPFSAVTLDVDHFKRINDRHGHDVGDQVLRHLAALIREQARHGDITGRIGG 432 Query: 198 EEFVILLARTRPAEAFNVAERVRRALEQRKGPIPTQVTVSQGIAHYPQHASEQAELLKKA 257 EEF++LL P AF AER+R A+E R P VTVS G+AHYPQ A E +LK + Sbjct: 433 EEFILLLPDATPDGAFRTAERLRLAVEARPNPAGQVVTVSLGVAHYPQSAGEVDAVLKLS 492 Query: 258 DQALYRAKSNGRNRTVVAE----DNPA 280 D ALY+AK +GRNRTVV + D PA Sbjct: 493 DLALYQAKRDGRNRTVVYQAERADGPA 519 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.135 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,561,788,149 Number of extensions: 58326101 Number of successful extensions: 213170 Number of sequences better than 1.0e-05: 14492 Number of HSP's gapped: 192824 Number of HSP's successfully gapped: 14527 Length of query: 284 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 150 Effective length of database: 1,798,280,193 Effective search space: 269742028950 Effective search space used: 269742028950 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)