BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0346.0039.p (341 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K3C5.1 B3K3C5_9BACI Alanine racemase EC=5.1.1.1 584 e-165 A5Z8A7.1 A5Z8A7_9FIRM Putative uncharacterized protein 233 2e-59 Q9CN88.1 PURR_PASMU HTH-type transcriptional repressor purR 201 6e-50 Q7VL44.1 PURR_HAEDU HTH-type transcriptional repressor purR 196 2e-48 Q0I4B4.1 PURR_HAES1 HTH-type transcriptional repressor purR 196 3e-48 >B3K3C5.1 B3K3C5_9BACI Alanine racemase EC=5.1.1.1 Length = 339 Score = 584 bits (1506), Expect = e-165, Method: Compositional matrix adjust. Identities = 277/339 (81%), Positives = 310/339 (91%) Query: 3 MGKITIRDVAREAGVSISTVSNALNDVDVLNPETKSHVLKVAQRLNYVPNLNGKQLKSGK 62 MGK+TI+DVAREAGVSISTVSNALNDVDVLNPETKSHVLKVA+RLNYVPNLNGK LKSGK Sbjct: 1 MGKVTIKDVAREAGVSISTVSNALNDVDVLNPETKSHVLKVAERLNYVPNLNGKLLKSGK 60 Query: 63 TMMLGFFTTSVAGPYFYKLVEAMSRQCDRLGYGLNVFVTKDKQVVMSNILGRRVDGVIIY 122 T MLGFFTTSV+GPYFY LVE+MSR+CDRLGYGLNVFVTKDKQV+MSNI+G RVDGVII+ Sbjct: 61 TKMLGFFTTSVSGPYFYTLVESMSRECDRLGYGLNVFVTKDKQVIMSNIMGGRVDGVIIF 120 Query: 123 EELGIGDKDIQAMNKEKIKAVFLDRVLENENMGSIIFDSFEAGYEATKYLIGLGHKKIAY 182 EEL I DKDI AM K+KIKAVFLDR ++NE MGSIIFDS+ AGYEATKYLI LGHKKIAY Sbjct: 121 EELKIDDKDIAAMEKDKIKAVFLDREVQNETMGSIIFDSYVAGYEATKYLISLGHKKIAY 180 Query: 183 ISGVDTMFDSVQRREGYLAALRQYGLPVSEEYILQGYFEEESTYNVVKSFIRAQPEKLPD 242 ISGVDTMFDSVQR+EGYLAALR+ G P+ +YILQGYFEEE +YN VKSF+ P K+PD Sbjct: 181 ISGVDTMFDSVQRKEGYLAALRECGFPIDGDYILQGYFEEEGSYNAVKSFLHLHPGKVPD 240 Query: 243 AFLAGNDLSAIGCIQALKAHGYEVPDDISVVGFDDIDIAQYYSPPLTTVRNQIARQGILA 302 AFLAGND+SAIGCI+ALK+HGYEVP D+SVVGFDDIDIAQY++PPL+TVRNQIARQGIL Sbjct: 241 AFLAGNDVSAIGCIRALKSHGYEVPQDVSVVGFDDIDIAQYFTPPLSTVRNQIARQGILV 300 Query: 303 IDHLVRMIQKQEQGNAQKLKGELIVRGSSHVKLGRNERI 341 +DHLVRMIQK+E+G QKL GEL+VRGSSHVK+ RNE + Sbjct: 301 VDHLVRMIQKKEKGKIQKLPGELVVRGSSHVKMSRNEPV 339 >A5Z8A7.1 A5Z8A7_9FIRM Putative uncharacterized protein Length = 335 Score = 233 bits (595), Expect = 2e-59, Method: Compositional matrix adjust. Identities = 131/332 (39%), Positives = 196/332 (59%), Gaps = 11/332 (3%) Query: 6 ITIRDVAREAGVSISTVSNALNDVDVLNPETKSHVLKVAQRLNYVPNLNGKQLKSGKTMM 65 +TI+DVAREAGV+ISTVSN N+ D+++ ETK VL+ ++L+Y+PN+N K LKS + Sbjct: 2 VTIKDVAREAGVAISTVSNVFNNGDIVSEETKQKVLEAVEKLHYIPNMNAKLLKSNRKNT 61 Query: 66 LGFFTTSVAGPYFYKLVEAMSRQCDRLGYGLNVFVTKDKQV--VMSNILGRRVDGVIIYE 123 +G F TS+ G ++ L +A+ QC Y LN++V+ D + I+ V G IIY Sbjct: 62 IGLFVTSLQGDFYNVLTQAIHLQCKLNNYMLNIYVSNDNSPDEIYRMIIASGVAGAIIYH 121 Query: 124 ELGIGD--KDIQAMNKEKIKAVFLDRVLENENMGSIIFDSFEAGYEATKYLIGLGHKKIA 181 E GD K I +N I VF+DR +++N+ ++ D A +LI LGHK+I Sbjct: 122 EDLTGDYVKRIATVN---IPMVFIDRDSKDKNISGVLVDDKLGATMAVDHLIKLGHKRIG 178 Query: 182 YISGVDTMFDSVQRREGYLAALRQYGLPVSEEYILQGYFEEESTYNVVKSFIRAQPEKLP 241 YI G +T D R +GY + + LP+ E+ IL+GY+EE Y+ ++S + + KLP Sbjct: 179 YIHGNET-GDDKHRFQGYKEMMEKNNLPIDEDIILRGYYEEALAYSEMRSMLSSGI-KLP 236 Query: 242 DAFLAGNDLSAIGCIQALKAHGYEVPDDISVVGFDDIDIAQYYSPPLTTVRNQIARQGIL 301 DA ND A GCIQAL+ G +VP+D+SV+G+DD ++ YY PLTTV + I G Sbjct: 237 DAMFCANDEMAWGCIQALQDVGIKVPEDVSVIGYDDSTLSAYYGVPLTTVHSPIVEMGSA 296 Query: 302 AIDHLVRMIQKQ--EQGNAQKLKGELIVRGSS 331 + + L R++ + + G KL ++ R S Sbjct: 297 SANELFRLLNSEGDKSGTVTKLSPTMVYRSSC 328 >Q9CN88.1 PURR_PASMU HTH-type transcriptional repressor purR Length = 334 Score = 201 bits (512), Expect = 6e-50, Method: Compositional matrix adjust. Identities = 118/334 (35%), Positives = 186/334 (55%), Gaps = 7/334 (2%) Query: 7 TIRDVAREAGVSISTVSNALNDVDVLNPETKSHVLKVAQRLNYVPNLNGKQLKSGKTMML 66 TI+DVA+ AGVS +TVS+ +N + ET+ VL+ Q LNY P+ + LK T + Sbjct: 3 TIKDVAKMAGVSTTTVSHVINKTRFVAAETEKLVLQAIQELNYSPSAVARSLKVNTTKSI 62 Query: 67 GFFTTSVAGPYFYKLVEAMSRQCDRLGYGLNVFVTKDKQVVMSN----ILGRRVDGVIIY 122 G TS PYF +++ A+ C R GY L + T++ + N ++ +RVDG+++ Sbjct: 63 GMIVTSSEAPYFAEIIHAVEEHCYRQGYSLFLCNTQNNVEKIKNHLEMLIKKRVDGILVM 122 Query: 123 EELGIGDKDIQAMNKEKIKAVFLDRVLENENMGSIIFDSFEAGYEATKYLIGLGHKKIAY 182 D N + V +D +N++ I SFE GY ATKYLI GHK I Sbjct: 123 CAEYTQDSLDLLANFTSLPLVVMDWGPDNKHTDIIQDHSFEGGYLATKYLIDHGHKDIGI 182 Query: 183 ISGVDTMFDSVQRREGYLAALRQYGLPVSEEYILQGYFEEESTYNVVKSFIRAQPEKLPD 242 I+G T + R EG++ A+ + GL ++ +++++G+FE E Y + I AQ + LP Sbjct: 183 IAGELTKTTAKTRYEGFIKAMNEAGLKINPDWVMEGFFEPEDGYECMNK-ILAQ-DTLPT 240 Query: 243 AFLAGNDLSAIGCIQALKAHGYEVPDDISVVGFDDIDIAQYYSPPLTTVRNQIARQGILA 302 A ND+ A+G I A+ G VP+DIS++G+D+I +++YSPPLTT+ +R G A Sbjct: 241 AVFCCNDVMALGAISAIGEKGLRVPEDISIIGYDNIHASRFYSPPLTTIHQSKSRLGARA 300 Query: 303 IDHLV-RMIQKQEQGNAQKLKGELIVRGSSHVKL 335 + L R+ +K E+ ++ EL++R S +L Sbjct: 301 VTLLFERINEKSEERAVIEMHPELVIRKSVKSRL 334 >Q7VL44.1 PURR_HAEDU HTH-type transcriptional repressor purR Length = 339 Score = 196 bits (498), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 113/331 (34%), Positives = 183/331 (55%), Gaps = 10/331 (3%) Query: 7 TIRDVAREAGVSISTVSNALNDVDVLNPETKSHVLKVAQRLNYVPNLNGKQLKSGKTMML 66 TI+DVA+ AGVS +TVS+ +N + ET V Q+LNY P+ + LK T + Sbjct: 3 TIKDVAKLAGVSTTTVSHVINKTRFVAEETSKTVWNAVQKLNYSPSAVARSLKINTTKSI 62 Query: 67 GFFTTSVAGPYFYKLVEAMSRQCDRLGYGLNVFVTKDKQVVMSN----ILGRRVDGVIIY 122 G T+ PYF ++V A+ C + GY L + T+++ + N ++ +RVDG+++ Sbjct: 63 GMIITTSEAPYFAEIVLAVEEHCYQQGYSLFLCNTQNEPEKIQNHLDMLIKKRVDGILVM 122 Query: 123 EELGIGDKDIQAMNKEKIKAVFLDRVLENENMGSIIFDSFEAGYEATKYLIGLGHKKIAY 182 ++ N I V +D +E II +SF Y AT++LI GHK I Sbjct: 123 CS-EYTRNSLELFNATNIPMVVMDWGKAHEKNDRIIDNSFNGAYLATRHLIDNGHKDIGV 181 Query: 183 ISGVDTMFDSVQRREGYLAALRQYGLPVSEEYILQGYFEEESTYNVVKSFIRAQPEKLPD 242 ISG T +R EG++ A+++ LP+ EE+IL+G FE ES + + + +R +KLP Sbjct: 182 ISGYLTKTICSERLEGFIKAMKEANLPIREEWILEGDFEPESGFEQMNNLLRL--DKLPT 239 Query: 243 AFLAGNDLSAIGCIQALKAHGYEVPDDISVVGFDDIDIAQYYSPPLTTVRNQIARQGILA 302 A +D A+G + AL G VP DIS++G+D+ ++++SPPLTT+ +R G+ A Sbjct: 240 AIFCFSDTIALGVLSALSEKGIAVPQDISIIGYDNTHSSRFFSPPLTTIHQSKSRLGVKA 299 Query: 303 IDHLVRMIQ---KQEQGNAQKLKGELIVRGS 330 ++ L+ I+ K+ Q + + EL++R S Sbjct: 300 LNLLLERIKLGHKEYQPHTIEFHPELVLRHS 330 >Q0I4B4.1 PURR_HAES1 HTH-type transcriptional repressor purR Length = 333 Score = 196 bits (498), Expect = 3e-48, Method: Compositional matrix adjust. Identities = 117/330 (35%), Positives = 187/330 (56%), Gaps = 9/330 (2%) Query: 7 TIRDVAREAGVSISTVSNALNDVDVLNPETKSHVLKVAQRLNYVPNLNGKQLKSGKTMML 66 TI+DVA+ AGVS +TVS+ +N + ET+ VL+ + LNY P+ + LK T + Sbjct: 3 TIKDVAKMAGVSTTTVSHVINKTRFVAKETEQQVLQAIKNLNYSPSAVARSLKVNTTKSI 62 Query: 67 GFFTTSVAGPYFYKLVEAMSRQCDRLGYGLNVFVTKDKQVVMSNILG----RRVDGVIIY 122 G T+ PYF +++ ++ C R GY L + T++ + N L +RVDG+++ Sbjct: 63 GMIVTTCETPYFAEIIHSVEELCYRQGYSLFLCNTQNNPEKIKNHLDMLAKKRVDGLLVM 122 Query: 123 -EELGIGDKDIQAMNKEKIKAVFLDRVLENENMGSIIFDSFEAGYEATKYLIGLGHKKIA 181 E ++ A E + V +D NEN I +SF GY ATKYLI GHK IA Sbjct: 123 CAEYTQNSLNLLA-TFEDLPMVVMDWGPFNENTDLIQDNSFSGGYIATKYLIDNGHKDIA 181 Query: 182 YISGVDTMFDSVQRREGYLAALRQYGLPVSEEYILQGYFEEESTYNVVKSFIRAQPEKLP 241 ISG +V R +G+ A+++ L ++ ++I++G+FE E Y + + +KLP Sbjct: 182 IISGELKKTTAVMRYQGFEKAMQEANLAINPDWIMEGFFEPEDGYECMNKILVQ--DKLP 239 Query: 242 DAFLAGNDLSAIGCIQALKAHGYEVPDDISVVGFDDIDIAQYYSPPLTTVRNQIARQGIL 301 A ND+ A+G I A+ G +VPDDISV+G+D+I ++++SPPLTT+ +R G+ Sbjct: 240 TAVFCCNDVMALGAISAIGEKGLKVPDDISVIGYDNIHASRFFSPPLTTIHQSKSRLGVQ 299 Query: 302 AIDHLVRMI-QKQEQGNAQKLKGELIVRGS 330 AI+ L + I +K ++ ++ EL++R S Sbjct: 300 AINLLFKRISEKGKEHEIIEIYPELVIRKS 329 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.137 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,924,074,043 Number of extensions: 78341726 Number of successful extensions: 241259 Number of sequences better than 1.0e-05: 9145 Number of HSP's gapped: 224996 Number of HSP's successfully gapped: 9172 Length of query: 341 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 205 Effective length of database: 1,779,438,163 Effective search space: 364784823415 Effective search space used: 364784823415 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)