BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0346.0035.p (290 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K3C9.1 B3K3C9_9BACI Glucan endo-1,6-beta-glucosidase EC=3.2.1.75 372 e-101 A7HJS9.1 A7HJS9_FERNB Glucan endo-1,6-beta-glucosidase EC=3.2.1.75 276 2e-72 B1DGE5.1 B1DGE5_9BACL Glycoside hydrolase family 30 273 2e-71 Q2TM40.1 Q2TM40_BIFBR Glycosyl hydrolase family 30 264 6e-69 C1UKM0.1 C1UKM0_9DELT O-glycosyl hydrolase 256 2e-66 >B3K3C9.1 B3K3C9_9BACI Glucan endo-1,6-beta-glucosidase EC=3.2.1.75 Length = 448 Score = 372 bits (956), Expect = e-101, Method: Compositional matrix adjust. Identities = 174/254 (68%), Positives = 204/254 (80%) Query: 4 LIMYRSTGEKGLFIEQPPEQQKVGASEQATVTVTIDESQTYQEMDGFGASFTDSAAYLIN 63 + +Y+S GE+ LF+++ Q + A V IDE QT+QEMDGFGASFTDSAAYLI+ Sbjct: 4 ITIYQSNGEEELFVKKDKAQLTPVTTGAAKHKVVIDEEQTFQEMDGFGASFTDSAAYLIH 63 Query: 64 RVLSPEQRTELMKRLFDPAAGIGLSVLRNPMGASDYARTVYSYNDLSEGETDMELNRFSV 123 +VL +QR+ELM +LFDP GIGLSVLR PMGASDYAR YSYND+ EG+TD+EL +FS+ Sbjct: 64 QVLETKQRSELMTQLFDPNEGIGLSVLRQPMGASDYARDFYSYNDMPEGQTDVELEQFSI 123 Query: 124 AHDEEDIIPLLQQAVALNPAVKLMASPWSAPGWMKTSGSMITGRLKPEYYGVYAGYFVRY 183 AHDEEDIIPLLQ+AV LNP VKLM SPWS PGWMKTSGSMI G LKPEYY VYA YFVRY Sbjct: 124 AHDEEDIIPLLQEAVRLNPDVKLMGSPWSPPGWMKTSGSMIGGELKPEYYSVYADYFVRY 183 Query: 184 IQAYAEHGLPVYAVTPQNEPLFEPHHYPGMLMLPEEQAMFVREYLKPAFVKHKINAKILC 243 IQ Y +GLP+YAVTPQNE L+ P HYPGM+MLPE Q+ F++ +LKP FVK+ I KILC Sbjct: 184 IQGYEANGLPIYAVTPQNEALYSPGHYPGMIMLPEAQSDFIKNHLKPQFVKNGIGTKILC 243 Query: 244 YDHNWDRPDYPLAV 257 YDHNWD+PDYPL V Sbjct: 244 YDHNWDKPDYPLTV 257 >A7HJS9.1 A7HJS9_FERNB Glucan endo-1,6-beta-glucosidase EC=3.2.1.75 Length = 484 Score = 276 bits (705), Expect = 2e-72, Method: Compositional matrix adjust. Identities = 131/254 (51%), Positives = 178/254 (70%), Gaps = 1/254 (0%) Query: 1 LQELIMYRSTGEKGLFIEQPPEQQKVGASEQATVTVTIDESQTYQEMDGFGASFTDSAAY 60 + + ++ +T ++ + + V + + T+ + +D ++ YQ+MDGFGAS TD++A+ Sbjct: 39 MNNVKLWLTTVDQKFMLSEVDINNPVVSDFRETLRIAVDSNKKYQQMDGFGASLTDASAW 98 Query: 61 LINRVLSPEQRTELMKRLFDPAAGIGLSVLRNPMGASDYARTVYSYNDLSEG-ETDMELN 119 LI LS E+R E+MK+LF GIG+S LR PMGA+DY +YSY+DL EG + D EL Sbjct: 99 LIFHKLSEEKRIEVMKKLFGRNEGIGISFLRQPMGATDYTTKLYSYDDLPEGVKEDPELK 158 Query: 120 RFSVAHDEEDIIPLLQQAVALNPAVKLMASPWSAPGWMKTSGSMITGRLKPEYYGVYAGY 179 FS+ HD++ IIPLL+ A+ +NP +K+MASPWSAPGWMKT+GSMI G L +YY VYA Y Sbjct: 159 YFSIKHDKQYIIPLLKLAMKINPELKIMASPWSAPGWMKTTGSMIGGSLLRQYYSVYAQY 218 Query: 180 FVRYIQAYAEHGLPVYAVTPQNEPLFEPHHYPGMLMLPEEQAMFVREYLKPAFVKHKINA 239 FV++ +AY + G+P+YA+TPQNEPL+ P YPGM M EEQA F+ EYL PAF K I Sbjct: 219 FVKFTKAYEKEGIPIYAITPQNEPLYVPKEYPGMKMTWEEQADFIGEYLGPAFEKEGIKT 278 Query: 240 KILCYDHNWDRPDY 253 KIL YDHNWD Y Sbjct: 279 KILTYDHNWDNTIY 292 >B1DGE5.1 B1DGE5_9BACL Glycoside hydrolase family 30 Length = 1637 Score = 273 bits (698), Expect = 2e-71, Method: Compositional matrix adjust. Identities = 125/253 (49%), Positives = 177/253 (69%), Gaps = 2/253 (0%) Query: 6 MYRSTGEKGLFIEQPPEQQKVGASEQATVTVTIDESQTYQEMDGFGASFTDSAAYLINRV 65 ++ +TG+K I + P+ A +T+ +DE+ YQ +DGFG + TDS+AY+I++ Sbjct: 37 VWMTTGDKSNLITKQPDVAFETNGAPAELTIDVDENTKYQTIDGFGGALTDSSAYVISQ- 95 Query: 66 LSPEQRTELMKRLFD-PAAGIGLSVLRNPMGASDYARTVYSYNDLSEGETDMELNRFSVA 124 + + + LM +LF G+G S +R PMG SD+A ++Y+Y+D+ +GETD +L FSVA Sbjct: 96 MDKQAKDALMNKLFGRDGDGVGFSYMRLPMGTSDFATSIYTYDDMPKGETDPDLEHFSVA 155 Query: 125 HDEEDIIPLLQQAVALNPAVKLMASPWSAPGWMKTSGSMITGRLKPEYYGVYAGYFVRYI 184 HD IIP L+QA+ +NP +K+M +PWSAPGWMKT+ S + G+LK EYYGVYA YFV++I Sbjct: 156 HDSAYIIPFLKQALQINPDLKIMGTPWSAPGWMKTTDSSVKGKLKEEYYGVYAQYFVKFI 215 Query: 185 QAYAEHGLPVYAVTPQNEPLFEPHHYPGMLMLPEEQAMFVREYLKPAFVKHKINAKILCY 244 +AY G+P+ A+T QNEP FEP YPGM M P +QA FV++YL PAF KI+ KI+ + Sbjct: 216 EAYEAEGVPIDAITLQNEPHFEPADYPGMRMEPVDQAKFVKDYLGPAFENAKIDTKIIVW 275 Query: 245 DHNWDRPDYPLAV 257 DHNW PDYP+ V Sbjct: 276 DHNWSEPDYPIEV 288 >Q2TM40.1 Q2TM40_BIFBR Glycosyl hydrolase family 30 Length = 443 Score = 264 bits (675), Expect = 6e-69, Method: Compositional matrix adjust. Identities = 129/254 (50%), Positives = 174/254 (68%), Gaps = 8/254 (3%) Query: 6 MYRSTGEKGLFIEQPPEQQKVGASEQAT--VTVTIDESQTYQEMDGFGASFTDSAAYLIN 63 ++R+ ++ L +P EQ AS + T V + + +T Q + GFGAS TDS+AYL+N Sbjct: 5 IFRTISQRHLM--EPIEQ----ASHEGTFDVEIMMKPGETMQRVVGFGASLTDSSAYLLN 58 Query: 64 RVLSPEQRTELMKRLFDPAAGIGLSVLRNPMGASDYARTVYSYNDLSEGETDMELNRFSV 123 L E R + M LFDP GIGLS+LRNPMGASDYAR VYSY+D+ +G+ D + FS+ Sbjct: 59 ETLDDESRKQAMTDLFDPDQGIGLSMLRNPMGASDYARDVYSYDDMPDGKRDDSMEHFSI 118 Query: 124 AHDEEDIIPLLQQAVALNPAVKLMASPWSAPGWMKTSGSMITGRLKPEYYGVYAGYFVRY 183 A DE ++IPL+ +A LNP +K++ SPWSAP WMKT+GSM G L+ + YA YFV+ Sbjct: 119 ARDERNVIPLIHEAKRLNPDLKVVMSPWSAPAWMKTNGSMKAGSLREDCRETYARYFVQC 178 Query: 184 IQAYAEHGLPVYAVTPQNEPLFEPHHYPGMLMLPEEQAMFVREYLKPAFVKHKINAKILC 243 ++AY HG+ V+AVTPQNEPL+EP HYP M M +E+ +FVR++L+ A + IL Sbjct: 179 LRAYRSHGIEVHAVTPQNEPLYEPTHYPSMAMSAQEETVFVRDHLRTAMREAGFKTLILG 238 Query: 244 YDHNWDRPDYPLAV 257 YDHNWDR DYPLA+ Sbjct: 239 YDHNWDRCDYPLAL 252 >C1UKM0.1 C1UKM0_9DELT O-glycosyl hydrolase Length = 627 Score = 256 bits (654), Expect = 2e-66, Method: Compositional matrix adjust. Identities = 123/253 (48%), Positives = 164/253 (64%), Gaps = 2/253 (0%) Query: 2 QELIMYRSTGEKGLFIEQPPEQQKVGASEQATVTVTIDESQTYQEMDGFGASFTDSAAYL 61 + + ++ +TG++ + Q GA VT+T+DE YQ MDGFGA+ T S+AYL Sbjct: 48 ESVAVWVTTGDRSKLLAQQASV-NFGADTSQPVTITVDEQVRYQTMDGFGAAMTGSSAYL 106 Query: 62 INRVLSPEQRTELMKRLFDPAAGIGLSVLRNPMGASDYARTVYSYNDLSEGETDMELNRF 121 +NR +S QR L+ LF P GIGLS++R +GASD++ + Y+YND++ G++D LN F Sbjct: 107 MNRAMSASQREALLSDLFSPG-GIGLSMVRQTIGASDFSLSSYTYNDMAPGQSDPNLNNF 165 Query: 122 SVAHDEEDIIPLLQQAVALNPAVKLMASPWSAPGWMKTSGSMITGRLKPEYYGVYAGYFV 181 S++ D +D++P+LQ A A N A+K+M SPWSAP WMK ++ G L +YG YA Y V Sbjct: 166 SLSGDLDDVVPMLQAARAKNGALKIMGSPWSAPAWMKEVHTLNGGWLNVAWYGAYADYLV 225 Query: 182 RYIQAYAEHGLPVYAVTPQNEPLFEPHHYPGMLMLPEEQAMFVREYLKPAFVKHKINAKI 241 +YIQAY GLP+YA+T QNEPL E YP M M P QA FV+ L PA I KI Sbjct: 226 KYIQAYQARGLPIYAITIQNEPLHETGSYPSMRMDPANQANFVKNNLGPAMSAAGIGTKI 285 Query: 242 LCYDHNWDRPDYP 254 L YDHNWDR DYP Sbjct: 286 LAYDHNWDRWDYP 298 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.135 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,777,123,247 Number of extensions: 73005298 Number of successful extensions: 164675 Number of sequences better than 1.0e-05: 302 Number of HSP's gapped: 163973 Number of HSP's successfully gapped: 304 Length of query: 290 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 156 Effective length of database: 1,798,280,193 Effective search space: 280531710108 Effective search space used: 280531710108 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)