BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0346.0034.p (169 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K3C9.1 B3K3C9_9BACI Glucan endo-1,6-beta-glucosidase EC=3.2.1.75 245 1e-63 Q2TM40.1 Q2TM40_BIFBR Glycosyl hydrolase family 30 129 8e-29 A7HJS9.1 A7HJS9_FERNB Glucan endo-1,6-beta-glucosidase EC=3.2.1.75 127 5e-28 B0T6Y0.1 B0T6Y0_CAUSK Glycoside hydrolase family 30 115 2e-24 Q9A7G6.1 Q9A7G6_CAUCR Glycosyl hydrolase, family 30 110 6e-23 >B3K3C9.1 B3K3C9_9BACI Glucan endo-1,6-beta-glucosidase EC=3.2.1.75 Length = 448 Score = 245 bits (625), Expect = 1e-63, Method: Compositional matrix adjust. Identities = 115/162 (70%), Positives = 138/162 (85%), Gaps = 1/162 (0%) Query: 4 AFEDKEVHFTEGSGGEWIPPFEQAFSNVIRTGIEILRNHSKSFVLWNMALDEQNGPAVPG 63 AF KEVHFTEGSGGEWIPPFEQAFSNV+RTGIEILRNHSKS+VLWNMALDE++GP VPG Sbjct: 286 AFPGKEVHFTEGSGGEWIPPFEQAFSNVMRTGIEILRNHSKSYVLWNMALDEKSGPTVPG 345 Query: 64 FGRSTCRGIVRIDQQTRELTYTLDYYALAHFSKVIRPKALRLESHSTHPAVTTAAFRNLD 123 FG+STCRGIV ++QQT+ELTYTLDYYALAHFSKVIRPKA R+ S ++H + + AF+N D Sbjct: 346 FGKSTCRGIVTVNQQTKELTYTLDYYALAHFSKVIRPKARRVAS-TSHEFIRSVAFKNTD 404 Query: 124 GSVGAVLFNDGEELETVAVRLPGEEPLTFSMAPKSAVSLLVK 165 GS+ AVLFND E E+VA++L GEE L M KSA+++ ++ Sbjct: 405 GSIAAVLFNDSEAPESVAIQLRGEEALQLEMPAKSALTVKIE 446 >Q2TM40.1 Q2TM40_BIFBR Glycosyl hydrolase family 30 Length = 443 Score = 129 bits (325), Expect = 8e-29, Method: Compositional matrix adjust. Identities = 71/162 (43%), Positives = 100/162 (61%), Gaps = 7/162 (4%) Query: 8 KEVHFTEGSGGEWIPPFEQAFSNVIRTGIEILRNHSKSFVLWNMALDEQNGPAVPGFGRS 67 K + TE SGGEWIP FE AFS+++ I+ L + + +FVLWN+ALDE GP VPGFG S Sbjct: 285 KLSYVTEASGGEWIPGFEPAFSHLVGMIIQALNHGANAFVLWNIALDEHRGPTVPGFGES 344 Query: 68 TCRGIVRIDQQTRELTYTLDYYALAHFSKVIRPKAL---RLESHSTHPAVTTAAFRNLDG 124 TC G++R+D + R+ + +DYY LAHFS+ IRP A + + +T A AA N DG Sbjct: 345 TCGGLLRVDSERRKASKEIDYYGLAHFSRHIRPGAHVVPTIATGNTDGARCVAAL-NEDG 403 Query: 125 SVGAVLFNDGEELETVAVRLPGEE-PLTFSMAPKSAVSLLVK 165 S VL NDGE + VR E+ ++ PK+ +++ + Sbjct: 404 SRAMVLLNDGE--SPITVRAGWEDNAANVTLTPKAVATIVWR 443 >A7HJS9.1 A7HJS9_FERNB Glucan endo-1,6-beta-glucosidase EC=3.2.1.75 Length = 484 Score = 127 bits (318), Expect = 5e-28, Method: Compositional matrix adjust. Identities = 63/148 (42%), Positives = 91/148 (61%), Gaps = 3/148 (2%) Query: 1 VKLAFEDKEVHFTEGSGGEWIPPFEQAFSNVIRTGIEILRNHSKSFVLWNMALDEQNGPA 60 +K F DK++ FTEGSGG+W+P F AF + + I I RN SK+ V WN+ALDE+ GP Sbjct: 324 IKEMFPDKDIWFTEGSGGDWVPAFFNAFMDQMMHVIRIPRNWSKTVVWWNIALDEKRGPT 383 Query: 61 VPGFGRSTCRGIVRIDQQTRELTYTLDYYALAHFSKVIRPKALRLESHSTHPAVTTAAFR 120 + STCRG++ I+Q+T E+ Y LDYY L H SK + P A R++S+ T+ + T AF Sbjct: 384 I--LSNSTCRGLIEINQETGEVKYNLDYYTLGHISKFVLPGAYRIDSY-TYSNLETVAFE 440 Query: 121 NLDGSVGAVLFNDGEELETVAVRLPGEE 148 N +G+ ++ N + + V G E Sbjct: 441 NPNGTKVLIVSNRTNTNKKIIVEEGGRE 468 >B0T6Y0.1 B0T6Y0_CAUSK Glycoside hydrolase family 30 Length = 480 Score = 115 bits (287), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 65/162 (40%), Positives = 95/162 (58%), Gaps = 13/162 (8%) Query: 1 VKLAFEDKEVHFTEGSGGEWIPPFEQAFSNVIRT-GIEILRNHSKSFVLWNMALDEQNGP 59 V A DK+V FTE SGG+W PF+++F ++R I RN ++ ++WN+ALDE +GP Sbjct: 320 VAGAHPDKDVFFTECSGGDWSGPFDESFGWLMRNLVIGSTRNGARGVLMWNLALDETHGP 379 Query: 60 AVPGFGRSTCRGIVRIDQQTRELTYTLDYYALAHFSKVIRPKALRLESHSTHPAVTTAAF 119 G G CRG+V ID +T +T +YYA H S+ +RP A+R++S T ++ + AF Sbjct: 380 HKGGCG--DCRGVVTIDSRTGAITRNPEYYAFGHASRFVRPGAVRIDSSETA-SLPSVAF 436 Query: 120 RNLDGSVGAVLFNDGEELETVAVR---------LPGEEPLTF 152 RN DG V+FN G++ + +VR LPG TF Sbjct: 437 RNPDGGRVLVVFNSGKDRQAFSVREGGRVAKTSLPGGAAATF 478 >Q9A7G6.1 Q9A7G6_CAUCR Glycosyl hydrolase, family 30 Length = 469 Score = 110 bits (274), Expect = 6e-23, Method: Compositional matrix adjust. Identities = 56/133 (42%), Positives = 82/133 (61%), Gaps = 3/133 (2%) Query: 1 VKLAFEDKEVHFTEGSGGEWIPPFEQAFSNVI-RTGIEILRNHSKSFVLWNMALDEQNGP 59 V+ A+ DKEV FTE SGGEW P F+ +F+ ++ + I R ++ ++WN+ALDE+ GP Sbjct: 308 VRAAYPDKEVFFTECSGGEWAPKFDDSFAWMVEQLIIGSTRGGARGVLMWNLALDEKFGP 367 Query: 60 AVPGFGRSTCRGIVRIDQQTRELTYTLDYYALAHFSKVIRPKALRLESHSTHPAVTTAAF 119 G G CRG+V ID QT LT T +YYA H S+ ++P A+R+ S + + T AF Sbjct: 368 HAGGCG--DCRGVVSIDSQTGALTRTQEYYAFGHASRFVKPDAVRIGSPAKVEGLRTVAF 425 Query: 120 RNLDGSVGAVLFN 132 +N DG ++ N Sbjct: 426 QNPDGQRVLIVLN 438 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.134 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,023,919,825 Number of extensions: 38457462 Number of successful extensions: 87958 Number of sequences better than 1.0e-05: 95 Number of HSP's gapped: 87893 Number of HSP's successfully gapped: 95 Length of query: 169 Length of database: 3,060,696,203 Length adjustment: 125 Effective length of query: 44 Effective length of database: 1,883,069,328 Effective search space: 82855050432 Effective search space used: 82855050432 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 126 (53.1 bits)