BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0346.0029.p (539 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KJ93.1 B3KJ93_9BACI Extracellular solute-binding protein family 1 773 0.0 B1D5F3.1 B1D5F3_9BACL Extracellular solute-binding protein family 1 748 0.0 A2RM82.1 A2RM82_LACLM Putative sugar ABC transporter substrate-b... 374 e-101 Q030Q1.1 Q030Q1_LACLS ABC-type sugar transport system, periplasm... 365 6e-99 B3K658.1 B3K658_9BACI Extracellular solute-binding protein family 1 308 8e-82 >B3KJ93.1 B3KJ93_9BACI Extracellular solute-binding protein family 1 Length = 539 Score = 773 bits (1996), Expect = 0.0, Method: Compositional matrix adjust. Identities = 375/536 (69%), Positives = 443/536 (82%), Gaps = 6/536 (1%) Query: 9 HSKSRAA-KKWPLLLLSAVMVMGMAGCSGNKEKADAPADT-GDFNKEGLPIVNEPVTLKV 66 H+K R K +++L+A++++ +AGCSG K K DA +D FNKEGLPIVNEP+TL V Sbjct: 5 HAKRRHRLKVAAVIMLAAILLLNLAGCSGGK-KEDAKSDAEAAFNKEGLPIVNEPITLDV 63 Query: 67 LTVRWGSMGDTFTQNQWLKDLEKNTNVKIDWQVMSSNDWAEQKSILLASGTLPDVVVGDQ 126 LTVRWG+MGDTF QNQWLKDLEK TNVKI+WQ+MSSNDW EQKSI+LASGTLPD++ GD Sbjct: 64 LTVRWGNMGDTFLQNQWLKDLEKETNVKINWQIMSSNDWGEQKSIMLASGTLPDIIFGDI 123 Query: 127 TFSDSDIVNNLSYFRPLDEYIDQYMPNLKAAMEETPEMKKISTFPDGKIYSLPTRLPSRP 186 FSDSDIVNNLSYFRPLD+YIDQYMPNLKAA+EETPEMKK+STFPDGKIYSLP RLPSRP Sbjct: 124 VFSDSDIVNNLSYFRPLDDYIDQYMPNLKAALEETPEMKKMSTFPDGKIYSLPARLPSRP 183 Query: 187 KSSRQPVINKAWLDKLGLKTPDTLDDLYNVMKAFKEKDPNGNGKADEIPYI---EVSNDL 243 K+S QP+INK WLD LGL PD D+LY V KAFKE DPNGNGKADEIP +S DL Sbjct: 184 KASIQPIINKTWLDALGLDVPDNTDELYEVFKAFKEGDPNGNGKADEIPVSGSGNISIDL 243 Query: 244 ISPFGITDLNSNNMLVKDGKAVYYPTSEEYKEGLKWENKLYSEGLLDKELFTQDATMISA 303 ++PFGITD+N N+M+V D + V+YP +E YKE +KW +KLY+EGL+D+E+FTQD+TM +A Sbjct: 244 LNPFGITDINGNSMMVIDNEPVFYPVTENYKEAIKWAHKLYAEGLIDQEIFTQDSTMSTA 303 Query: 304 KFQNPDAPIVGLSYQWTPDAVFGKWSEQYETIPPVAGPDGKRYTIGNPIGMNLARNELLI 363 K QNPDA ++G S QW PDAVFGKW +QY IP +AGPDGKRY G P GM+L RNE LI Sbjct: 304 KHQNPDAALIGFSNQWVPDAVFGKWKDQYIAIPSIAGPDGKRYQGGEPEGMSLRRNEFLI 363 Query: 364 TTSCKNPEVAARWADQFYTNEASIQNFWGAIGTVLKKNDDSTYTLMDPPAGTSADAWYWD 423 TTS K+PE++ARWADQFYTNEASIQNFWGAIGTV++KNDD T+TLM PP GTSADAWYW+ Sbjct: 364 TTSNKHPEISARWADQFYTNEASIQNFWGAIGTVIQKNDDGTFTLMSPPEGTSADAWYWE 423 Query: 424 QSLRDFGPKYVSPSFEKNIVLSQSXXXXXXXXXXXXXXXYVTTPFPNVMYTSEEFQELPT 483 QS+RDFGPKYVSPSFEKNI+L ++ YVTTPFPNVMY++EEFQELPT Sbjct: 424 QSVRDFGPKYVSPSFEKNIILDETTGDGLKLVIDKLGSDYVTTPFPNVMYSAEEFQELPT 483 Query: 484 LTTDIDSYVTTMRAQFITKGGIDEGWDAYVKKLNDMGLEKLLQIRTDAYARYMSVK 539 LTTDID +V T RA++I++G ID WDAYVKKLNDMGLE+L+QIR +AY RY+SVK Sbjct: 484 LTTDIDGFVGTTRAKWISEGNIDAEWDAYVKKLNDMGLERLVQIRKEAYQRYISVK 539 >B1D5F3.1 B1D5F3_9BACL Extracellular solute-binding protein family 1 Length = 546 Score = 748 bits (1932), Expect = 0.0, Method: Compositional matrix adjust. Identities = 360/531 (67%), Positives = 431/531 (81%), Gaps = 11/531 (2%) Query: 20 LLLLSAVMVMGMAGCSGNKEKADAPA--DTG------DFNKEGLPIVNEPVTLKVLTVRW 71 L L+S + +AGCS KE +A A +TG +F+ EGLPIV++PVTL VLT+RW Sbjct: 16 LALISVLGTTVLAGCSSGKETNNAAASNNTGSGKAGSNFHAEGLPIVDKPVTLDVLTIRW 75 Query: 72 GSMGDTFTQNQWLKDLEKNTNVKIDWQVMSSNDWAEQKSILLASGTLPDVVVGDQTFSDS 131 G+MGDTF +N WL++LEKN+NVKI+WQV+SS+DW QKS++LASG +PDV++GD F DS Sbjct: 76 GNMGDTFKKNAWLQELEKNSNVKINWQVISSSDWDAQKSVMLASGKMPDVIIGDLAFGDS 135 Query: 132 DIVNNLSYFRPLDEYIDQYMPNLKAAMEETPEMKKISTFPDGKIYSLPTRLPSRPKSSRQ 191 DIVNNLS FRPLD+YID+YMPNLKAAM E PE+KK+STFPDGKIYSLP RLPSRP SS Q Sbjct: 136 DIVNNLSIFRPLDDYIDKYMPNLKAAMAEMPELKKLSTFPDGKIYSLPARLPSRPYSSVQ 195 Query: 192 PVINKAWLDKLGLKTPDTLDDLYNVMKAFKEKDPNGNGKADEIPYIEVSN---DLISPFG 248 PVINK WLDKLGLK P+TLD+LY V+KAFKEKDPNGNGKADEIPY + + +L +PFG Sbjct: 196 PVINKEWLDKLGLKAPETLDELYTVLKAFKEKDPNGNGKADEIPYSDQKDLDMNLFAPFG 255 Query: 249 ITDLNSNNMLVKDGKAVYYPTSEEYKEGLKWENKLYSEGLLDKELFTQDATMISAKFQNP 308 ITD+ N+M++KDGK Y+PTSE YK + W NKLYSEGL+DKE FTQD TM++AK QNP Sbjct: 256 ITDIRGNHMVLKDGKPEYFPTSEAYKAAITWANKLYSEGLIDKETFTQDDTMLTAKRQNP 315 Query: 309 DAPIVGLSYQWTPDAVFGKWSEQYETIPPVAGPDGKRYTIGNPIGMNLARNELLITTSCK 368 DA +VG SYQWTPDAVFG+WS+QYETI P+ GPDGK+Y IG+P G+++ RNE+LITTS + Sbjct: 316 DAALVGFSYQWTPDAVFGQWSKQYETIAPITGPDGKKYQIGDPNGLSIRRNEVLITTSNE 375 Query: 369 NPEVAARWADQFYTNEASIQNFWGAIGTVLKKNDDSTYTLMDPPAGTSADAWYWDQSLRD 428 +PEVAARWADQFYT EASIQNFWGAIGT ++KN D TYTLMDPP GTSADAWYWD SLRD Sbjct: 376 HPEVAARWADQFYTGEASIQNFWGAIGTSVEKNADGTYTLMDPPDGTSADAWYWDSSLRD 435 Query: 429 FGPKYVSPSFEKNIVLSQSXXXXXXXXXXXXXXXYVTTPFPNVMYTSEEFQELPTLTTDI 488 FGPKYVSP FEKNI L + YVT PFP+VMYT EE+ ELPTL TDI Sbjct: 436 FGPKYVSPEFEKNIKLDPTSGDGLKLELDSLGKEYVTQPFPDVMYTEEEYAELPTLITDI 495 Query: 489 DSYVTTMRAQFITKGGIDEGWDAYVKKLNDMGLEKLLQIRTDAYARYMSVK 539 +SY+ + RA+++TKGGID+ WDAYVKKLNDMGLEKL++I DAY RYM++K Sbjct: 496 ESYIKSTRAKWVTKGGIDQEWDAYVKKLNDMGLEKLIKIYNDAYERYMNIK 546 >A2RM82.1 A2RM82_LACLM Putative sugar ABC transporter substrate-binding protein Length = 520 Score = 374 bits (959), Expect = e-101, Method: Compositional matrix adjust. Identities = 198/477 (41%), Positives = 285/477 (59%), Gaps = 12/477 (2%) Query: 66 VLTVRWGSMGDTFTQNQWLKDLEKNTNVKIDWQVMSSNDWAEQKSILLASGTLPDVVVGD 125 V TVRW G+ +T+ + L K + + W + DW ++KS L+A G LPD +G Sbjct: 43 VATVRWADWGNDYTK--FPNQLAKEAGINVKWDTYLNADWGDKKSTLMAGGNLPDTFMGS 100 Query: 126 QTFSDSDIVNNLSYFRPLDEYIDQYMPNLKAAMEETPEMKKISTFPDGKIYSLPTRLPSR 185 TF+D +I N S F PL++Y+ +PNL+ AM++ P +K + T PDG I+SLP P R Sbjct: 101 ITFTDQEIAQNQSNFIPLEKYVKD-IPNLQKAMKKNPALKAMITSPDGHIWSLPKEAPMR 159 Query: 186 PKSSRQPVINKAWLDKLGLKTPDTLDDLYNVMKAFKEKDPNGNGKADEIPY----IEVSN 241 P + Q INKAWLDKLGL+ P T D+ V++AFK KDPNGNGKADEIPY + + Sbjct: 160 PIIANQLFINKAWLDKLGLQMPTTYDEFVKVLEAFKTKDPNGNGKADEIPYGTGNFDPTM 219 Query: 242 DLISPFGITDL--NSNNMLVKDGKAVYYPTSEEYKEGLKWENKLYSEGLLDKELFTQDAT 299 PFG+ NSN M++ +GK Y +++ +K+G++ N Y++GL+D EL+T D T Sbjct: 220 SYTLPFGVLRGADNSNEMMMLNGKPAYIRSTDNFKKGIEAMNDAYTKGLIDPELYTLDPT 279 Query: 300 MISAKFQNPDAPIVGLSYQWTPDAVFGKWSEQYETIPPVAGPDGKRYTIGNPIGMNLARN 359 AK D VG+S WT DA FGK ++ Y +P + GPDG +Y + +P N R+ Sbjct: 280 QAQAKLM-ADTETVGVSVGWTADATFGKNAKDYVALPALKGPDGNQYVMSDPDHFNQGRD 338 Query: 360 ELLITTSCKNPEVAARWADQFYTNEASIQNFWGAIGTVLKKNDDSTYTLMDPPAGTSADA 419 E ++TT KN + +WAD+FYTN+A+IQN++GA G +K+ ++ Y ++ P SAD Sbjct: 339 EFMVTTHAKNVKALIKWADKFYTNDAAIQNYYGAFGVGTEKSGEA-YKVLKPTGNNSADT 397 Query: 420 WYWDQSLRDFGPKYVSPSFEKNIVLSQ-SXXXXXXXXXXXXXXXYVTTPFPNVMYTSEEF 478 W SLRDFGPK S + + Y FP+V YT++E Sbjct: 398 QAWINSLRDFGPKSWDNSLNDKVTFEDPNNGDAQKLKMDAELKQYARPAFPSVTYTNDEL 457 Query: 479 QELPTLTTDIDSYVTTMRAQFITKGGIDEGWDAYVKKLNDMGLEKLLQIRTDAYARY 535 Q++ + D+ SY T A+++TKGGID+ W+AY+ KLN MGL+ L+I+TDAY RY Sbjct: 458 QKIAQVYPDLSSYTTQETAKWVTKGGIDKDWEAYIAKLNQMGLKDYLKIQTDAYERY 514 >Q030Q1.1 Q030Q1_LACLS ABC-type sugar transport system, periplasmic component Length = 520 Score = 365 bits (937), Expect = 6e-99, Method: Compositional matrix adjust. Identities = 195/477 (40%), Positives = 283/477 (59%), Gaps = 12/477 (2%) Query: 66 VLTVRWGSMGDTFTQNQWLKDLEKNTNVKIDWQVMSSNDWAEQKSILLASGTLPDVVVGD 125 V TVRW G+ +T+ + L K + + W + DW ++KS L+A G LPD +G Sbjct: 43 VATVRWADWGNDYTK--FPNQLAKEAGINVKWDTYLNTDWGDKKSTLMAGGNLPDAFMGS 100 Query: 126 QTFSDSDIVNNLSYFRPLDEYIDQYMPNLKAAMEETPEMKKISTFPDGKIYSLPTRLPSR 185 TF+D +I N S F PL++Y+ MPNL+ AM++ P +K + T PDG I+SLP P R Sbjct: 101 ITFTDQEIAQNQSNFIPLEKYVKD-MPNLQKAMKKNPALKAMITSPDGHIWSLPKEAPMR 159 Query: 186 PKSSRQPVINKAWLDKLGLKTPDTLDDLYNVMKAFKEKDPNGNGKADEIPY----IEVSN 241 P + Q INKAWLDKLGL+ P T D+ V++AFK KDPNGNGKADEIPY + + Sbjct: 160 PIIANQLFINKAWLDKLGLQMPTTYDEFVKVLEAFKTKDPNGNGKADEIPYGTGNFDPTM 219 Query: 242 DLISPFGITDL--NSNNMLVKDGKAVYYPTSEEYKEGLKWENKLYSEGLLDKELFTQDAT 299 P G+ NSN M++ +GK Y +++ +K+G++ + Y++GL+D EL+T D T Sbjct: 220 SYTLPLGVLRGADNSNEMMMLNGKPAYIRSTDNFKKGIEAMHDAYTKGLIDPELYTLDPT 279 Query: 300 MISAKFQNPDAPIVGLSYQWTPDAVFGKWSEQYETIPPVAGPDGKRYTIGNPIGMNLARN 359 AK D VG+S WT DA FGK ++ Y +P + GPDG +Y + + N R+ Sbjct: 280 QAQAKLM-ADTETVGVSVGWTADATFGKNAKDYVALPALKGPDGNQYVMSDSDHFNQGRD 338 Query: 360 ELLITTSCKNPEVAARWADQFYTNEASIQNFWGAIGTVLKKNDDSTYTLMDPPAGTSADA 419 E ++TT KN + +WAD+FYTN+A+IQN++GA G +K+ ++ Y ++ P SAD Sbjct: 339 EFMVTTHAKNVKALIKWADKFYTNDAAIQNYYGAFGVGTEKSGEA-YKVLKPTGDNSADT 397 Query: 420 WYWDQSLRDFGPKYVSPSFEKNIVL-SQSXXXXXXXXXXXXXXXYVTTPFPNVMYTSEEF 478 SLRDFGPK S + + + Y FP+V YT++E Sbjct: 398 QACINSLRDFGPKSWDNSLNDKVTFEAPTNGDAQKLKMDAELKQYARPAFPSVTYTNDEL 457 Query: 479 QELPTLTTDIDSYVTTMRAQFITKGGIDEGWDAYVKKLNDMGLEKLLQIRTDAYARY 535 Q++ + D+ SY T A+++TKGGID+ W+AY+ KLN MGL+ L+I+TDAY RY Sbjct: 458 QKIAQVYPDLSSYTTQETAKWVTKGGIDKDWEAYIAKLNQMGLKDYLKIQTDAYERY 514 >B3K658.1 B3K658_9BACI Extracellular solute-binding protein family 1 Length = 537 Score = 308 bits (789), Expect = 8e-82, Method: Compositional matrix adjust. Identities = 200/536 (37%), Positives = 291/536 (54%), Gaps = 25/536 (4%) Query: 16 KKWPLLLLSAVMVMGMAGCSGN-KEKADAPADTGDFNKEGLPIVNEPVTLKVLTVRWGSM 74 K+ +L+L+ ++GCSG+ K ++D P +FNK GLPIVNEPV+LK+++ + ++ Sbjct: 6 KRAGILMLAGAF--ALSGCSGDAKNQSDNPDQAANFNKTGLPIVNEPVSLKMVSPK-AAL 62 Query: 75 GDTFTQNQWLKDLEKNTNVKIDWQVMSSNDWAEQKSILLASGTLPDVVVGDQTFSDSDIV 134 ++Q + K LE TNVKI+W+ + D+ E+K++LLASG LPD G FSD ++V Sbjct: 63 APEYSQMEIFKRLEAETNVKIEWENIPDTDFTEKKNLLLASGNLPDAFYG-AGFSDYELV 121 Query: 135 --NNLSYFRPLDEYIDQYMPNLKAAMEETPEMKKISTFPDGKIYSLPT----RLPSRPKS 188 PL++ IDQY PNLKA ++ P++K T PDG IY LP+ L + P Sbjct: 122 TYGEDGTILPLEDLIDQYAPNLKALLDRRPDIKSSITAPDGHIYGLPSWEENNLGTNPFF 181 Query: 189 SRQPVINKAWLDKLGLKTPDTLDDLYNVMKAFKEKDPNGNGKADEIP----YIEVSNDLI 244 VINK WLDKLGLK P TLD+ + AFK +DPNGNGKADEIP +++ D+ Sbjct: 182 H---VINKNWLDKLGLKMPQTLDEYTEALVAFKTQDPNGNGKADEIPLSFMHMQWCMDIA 238 Query: 245 SPFGITDL--NSNNMLVKDGKAVYYPTSEEYKEGLKW-ENKLYSEGLLDKELFTQDATMI 301 FG L N + +V+DGK ++ T EYKE LK+ K Y +GL+D E FTQDA Sbjct: 239 GIFGAFGLPDNMEHRIVRDGKVIFTATQPEYKEALKYFHEKWYKQGLIDPESFTQDAAQY 298 Query: 302 SAKFQNPDAPIVGLSYQWTPDAVFG-KWSEQYETIPPVAGPDGKRYTIGNPIGMNLARNE 360 AK + +G W D V G + SE Y + P+ GP+G + IG G R Sbjct: 299 LAKGKT-STETLGSYIWWEVDEVVGPERSEDYALLSPLKGPNGDQ-LIGRGNGGGPGRGA 356 Query: 361 LLITTSCKNPEVAARWADQFYTNEASIQNFWGAIGTVLKKNDDSTYTLMDPPAGTSADAW 420 +IT KNPE+ RW DQ Y + Q WG + V +K+++ + P GTSA + Sbjct: 357 FVITKENKNPEITMRWIDQQYEPYMAAQIHWGPLDIVFEKDENGKLVNLPLPEGTSAGEF 416 Query: 421 YWDQSLRDFGPKYVSPSFEKNIVLSQSXXXXXXXXXXXXXXXYVTTP-FPNVMYTSEEFQ 479 + GP ++ +V + Y+ +P++ + EE Sbjct: 417 RQKVAPGAGGPGVITIEDLGKVVDLEPRAQQRVKDLEKYYDPYMEKENYPSIFFEPEELD 476 Query: 480 ELPTLTTDIDSYVTTMRAQFITKGGIDEGWDAYVKKLNDMGLEKLLQIRTDAYARY 535 ++ + ++ YV T R +FI GG+DE WD+YVK L MGL +L++I + RY Sbjct: 477 KINRIEPELIKYVNTQRGKFIVDGGVDEQWDSYVKTLEKMGLNELMEIYQNGLDRY 532 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.314 0.132 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,407,311,067 Number of extensions: 149669631 Number of successful extensions: 267836 Number of sequences better than 1.0e-05: 335 Number of HSP's gapped: 267138 Number of HSP's successfully gapped: 342 Length of query: 539 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 398 Effective length of database: 1,732,333,088 Effective search space: 689468569024 Effective search space used: 689468569024 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 134 (56.2 bits)