BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0346.0028.p (268 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KJ91.1 B3KJ91_9BACI Binding-protein-dependent transport system... 438 e-121 B1D5F5.1 B1D5F5_9BACL Binding-protein-dependent transport system... 416 e-114 Q030Q3.1 Q030Q3_LACLS ABC-type polysaccharide transport system, ... 315 3e-84 B3K9I4.1 B3K9I4_9BACI Binding-protein-dependent transport system... 296 2e-78 B3KC81.1 B3KC81_9BACI Binding-protein-dependent transport system... 295 2e-78 >B3KJ91.1 B3KJ91_9BACI Binding-protein-dependent transport systems inner membrane component Length = 309 Score = 438 bits (1126), Expect = e-121, Method: Compositional matrix adjust. Identities = 210/268 (78%), Positives = 236/268 (88%) Query: 1 MYGVLIAFKDYSPALGITDSPWAGFKYFEKFFHSYQFSSTIKNTLIISVYSLITFPIPVM 60 MYG+LIAFKDYSPALGI DSPWAG KYFEKFF+SYQFS+TI NTL+IS+YS+ TFPIP+M Sbjct: 42 MYGILIAFKDYSPALGIMDSPWAGLKYFEKFFNSYQFSTTITNTLVISLYSVATFPIPIM 101 Query: 61 LALMVNQMRPNRFRRFFQTVSYMPHFISTVVMVGLMMILLSPSTGLVGNLYSLFGKEAPD 120 LALMVNQMR N F+RFFQTV+YMPHFISTVVMVGLM+IL SP +GL+GN++ LFG EAP+ Sbjct: 102 LALMVNQMRQNWFKRFFQTVTYMPHFISTVVMVGLMLILFSPGSGLIGNIFRLFGAEAPN 161 Query: 121 LMGSAGWFSSVYVWSDVWQHVGWDSIIFIAALSAGDPSLYEAATVDGASRWHKVRYIDIP 180 LMGSAG FSSVYVWSDVWQH GWDSII+IAALSA PSLYEAAT+DGASRW K+R+IDIP Sbjct: 162 LMGSAGLFSSVYVWSDVWQHTGWDSIIYIAALSAVSPSLYEAATMDGASRWQKIRFIDIP 221 Query: 181 MLMPTAITXXXXXXXXXXXXXFEKVYLMQNDLNATSSEILSTYVYKIGLLSSQYSFSSAI 240 MLMPT +T FEKVYLMQN LN TSSE++STYVYKIG+LSSQYSFSSAI Sbjct: 222 MLMPTVVTLLILRIGSLLGVGFEKVYLMQNSLNITSSEVISTYVYKIGMLSSQYSFSSAI 281 Query: 241 NLFNTVINFILLIMVNQISKKLSENSLW 268 NLFNT+INF+LLI+VNQISKK SENSLW Sbjct: 282 NLFNTIINFVLLILVNQISKKYSENSLW 309 >B1D5F5.1 B1D5F5_9BACL Binding-protein-dependent transport systems inner membrane component Length = 309 Score = 416 bits (1069), Expect = e-114, Method: Compositional matrix adjust. Identities = 196/268 (73%), Positives = 228/268 (85%) Query: 1 MYGVLIAFKDYSPALGITDSPWAGFKYFEKFFHSYQFSSTIKNTLIISVYSLITFPIPVM 60 MYGV+IAFKD+ PALGI DSPW GFKYFEKFFHSYQF+ TIKNT+ IS+YS +TFP+P+ Sbjct: 42 MYGVIIAFKDFKPALGIMDSPWVGFKYFEKFFHSYQFTVTIKNTVFISLYSFVTFPLPIA 101 Query: 61 LALMVNQMRPNRFRRFFQTVSYMPHFISTVVMVGLMMILLSPSTGLVGNLYSLFGKEAPD 120 A++VNQ+R NRF+R FQT++YMPHFIST+V+VGLMMILLSP GL+GN+Y LFG EAP+ Sbjct: 102 FAILVNQLRQNRFKRVFQTITYMPHFISTIVIVGLMMILLSPGNGLIGNIYRLFGAEAPN 161 Query: 121 LMGSAGWFSSVYVWSDVWQHVGWDSIIFIAALSAGDPSLYEAATVDGASRWHKVRYIDIP 180 LMGSA FSS+YVWSDVWQH GWDSII++AALS+ PSLYEAATVDGASRW K+RYIDIP Sbjct: 162 LMGSASMFSSLYVWSDVWQHTGWDSIIYLAALSSVSPSLYEAATVDGASRWQKIRYIDIP 221 Query: 181 MLMPTAITXXXXXXXXXXXXXFEKVYLMQNDLNATSSEILSTYVYKIGLLSSQYSFSSAI 240 ML+PTA+T FEK YL+QN+LN SE++STYVYKIGLLSSQYSFS+AI Sbjct: 222 MLIPTAVTLLILRIGGLLGVGFEKAYLLQNNLNIGGSEVISTYVYKIGLLSSQYSFSAAI 281 Query: 241 NLFNTVINFILLIMVNQISKKLSENSLW 268 NLFNTVINF LLI+VN ISKK SENSLW Sbjct: 282 NLFNTVINFTLLILVNWISKKYSENSLW 309 >Q030Q3.1 Q030Q3_LACLS ABC-type polysaccharide transport system, permease component Length = 316 Score = 315 bits (807), Expect = 3e-84, Method: Compositional matrix adjust. Identities = 150/269 (55%), Positives = 206/269 (76%), Gaps = 1/269 (0%) Query: 1 MYGVLIAFKDYSPALGITDSPWAGFKYFEKFFHSYQFSSTIKNTLIISVYSLI-TFPIPV 59 MYG+L+AFKDYSPALG++ SPW GFK+F ++F+SYQF TI NTL I+VYS++ F +P+ Sbjct: 48 MYGLLMAFKDYSPALGVSGSPWVGFKWFIQYFNSYQFWPTILNTLKIAVYSIVFGFTLPI 107 Query: 60 MLALMVNQMRPNRFRRFFQTVSYMPHFISTVVMVGLMMILLSPSTGLVGNLYSLFGKEAP 119 LAL+ NQ+R F++FFQ +Y+PHFIST+VM G++++ LSPS GLV N +F + P Sbjct: 108 ALALICNQIRVQAFKKFFQVTTYLPHFISTMVMCGMILLFLSPSNGLVANFLGIFNLKMP 167 Query: 120 DLMGSAGWFSSVYVWSDVWQHVGWDSIIFIAALSAGDPSLYEAATVDGASRWHKVRYIDI 179 D++G+ F+S+YVWSDVWQHVGW+SII++AALSA DP+ YEAAT+DGASR ++ ID+ Sbjct: 168 DVIGNPNAFASLYVWSDVWQHVGWNSIIYLAALSAIDPTYYEAATMDGASRLQQMIKIDL 227 Query: 180 PMLMPTAITXXXXXXXXXXXXXFEKVYLMQNDLNATSSEILSTYVYKIGLLSSQYSFSSA 239 P+L+PT + FEKV L+QN LN + SEI+STYVYK+GL S QYS+S+A Sbjct: 228 PLLVPTMMIMLILAVGQLLNIGFEKVLLLQNPLNLSGSEIISTYVYKVGLKSFQYSYSTA 287 Query: 240 INLFNTVINFILLIMVNQISKKLSENSLW 268 INLF TVIN ++L+ VN IS++L++ L+ Sbjct: 288 INLFQTVINLVILVTVNFISRRLTKTGLF 316 >B3K9I4.1 B3K9I4_9BACI Binding-protein-dependent transport systems inner membrane component Length = 323 Score = 296 bits (757), Expect = 2e-78, Method: Compositional matrix adjust. Identities = 141/269 (52%), Positives = 193/269 (71%), Gaps = 1/269 (0%) Query: 1 MYGVLIAFKDYSPALGITDSPWAGFKYFEKFFHSYQFSSTIKNTLIISVYSL-ITFPIPV 59 MYGV IAFKD+ GI DSPW GFK+FE+FF S+ F IKNT+ I +Y L + FPIP+ Sbjct: 55 MYGVQIAFKDFMANKGIMDSPWVGFKHFERFFDSFYFWRIIKNTMGIGLYELAVGFPIPI 114 Query: 60 MLALMVNQMRPNRFRRFFQTVSYMPHFISTVVMVGLMMILLSPSTGLVGNLYSLFGKEAP 119 +LALM+N+ R RFRRF QTV+Y PHF+STVV+VG++M+ LSP +GLV + + FG E Sbjct: 115 ILALMINEARSGRFRRFVQTVTYAPHFLSTVVVVGMIMMFLSPVSGLVNLIITSFGGEPI 174 Query: 120 DLMGSAGWFSSVYVWSDVWQHVGWDSIIFIAALSAGDPSLYEAATVDGASRWHKVRYIDI 179 M WF S+YVWS VWQ +GW SII++AAL+ DP L+EAA VDGA ++ +I++ Sbjct: 175 AFMTEPSWFKSIYVWSGVWQGMGWSSIIYLAALAGIDPQLHEAAKVDGAGWLRRIWHINL 234 Query: 180 PMLMPTAITXXXXXXXXXXXXXFEKVYLMQNDLNATSSEILSTYVYKIGLLSSQYSFSSA 239 P + PT FEK++LMQN LN +S++++T VY+ G+L +QYSFS+A Sbjct: 235 PGIAPTMTILLILNIGSVLGVGFEKIFLMQNSLNMEASDVIATNVYRSGILGAQYSFSAA 294 Query: 240 INLFNTVINFILLIMVNQISKKLSENSLW 268 + LFN+++NFI+LI VN+I++K+SENSLW Sbjct: 295 VGLFNSIVNFIMLITVNRIARKVSENSLW 323 >B3KC81.1 B3KC81_9BACI Binding-protein-dependent transport systems inner membrane component Length = 319 Score = 295 bits (756), Expect = 2e-78, Method: Compositional matrix adjust. Identities = 143/269 (53%), Positives = 198/269 (73%), Gaps = 1/269 (0%) Query: 1 MYGVLIAFKDYSPALGITDSPWAGFKYFEKFFHSYQFSSTIKNTLIISVYSL-ITFPIPV 59 MYG+ IAFKD+ GI SPW GF++F++FF+SY F IKNTL I +YSL + FPIP+ Sbjct: 51 MYGIQIAFKDFIATQGIWGSPWVGFEHFDRFFNSYYFWRLIKNTLGIGLYSLAVGFPIPI 110 Query: 60 MLALMVNQMRPNRFRRFFQTVSYMPHFISTVVMVGLMMILLSPSTGLVGNLYSLFGKEAP 119 +LAL++N++R RF++F QT++Y PHF+STVV+VG+MMI LSP G++ + +FG + Sbjct: 111 ILALLMNEVRAERFKKFVQTITYAPHFLSTVVVVGMMMIFLSPRYGIINHFIEMFGGQPI 170 Query: 120 DLMGSAGWFSSVYVWSDVWQHVGWDSIIFIAALSAGDPSLYEAATVDGASRWHKVRYIDI 179 + M WF S+YV SDVWQ +GW SII++AAL+ D L+EAA VDGA+R ++ +I+I Sbjct: 171 NFMTEPSWFKSLYVLSDVWQTMGWSSIIYLAALAGIDNQLHEAARVDGATRLQRIWHINI 230 Query: 180 PMLMPTAITXXXXXXXXXXXXXFEKVYLMQNDLNATSSEILSTYVYKIGLLSSQYSFSSA 239 P +MPT I FEKV LMQN+LN SS+I++TYVY++G+ +++YSFS+A Sbjct: 231 PGIMPTIIILLILNMGSIMGIGFEKVLLMQNNLNMESSDIIATYVYRMGIQNAEYSFSAA 290 Query: 240 INLFNTVINFILLIMVNQISKKLSENSLW 268 I LFN+VINFILL+ VN ISK++SE SLW Sbjct: 291 IGLFNSVINFILLVAVNFISKRVSETSLW 319 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.325 0.137 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,425,464,295 Number of extensions: 54512066 Number of successful extensions: 202120 Number of sequences better than 1.0e-05: 4200 Number of HSP's gapped: 199422 Number of HSP's successfully gapped: 4201 Length of query: 268 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 135 Effective length of database: 1,807,701,208 Effective search space: 244039663080 Effective search space used: 244039663080 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 130 (54.7 bits)