BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0346.0014.p (394 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q9AJR6.2 Q9AJR6_BACGL Alpha-D-mannosidase 448 e-124 A4IPC6.1 A4IPC6_GEOTN Alpha-D-mannosidase 442 e-122 B4BM85.1 B4BM85_9BACI Alpha-mannosidase EC=3.2.1.24 441 e-122 Q5KYP9.1 Q5KYP9_GEOKA Alpha-D-mannosidase EC=3.2.1.24 438 e-121 A9KS60.1 A9KS60_CLOPH Glycoside hydrolase family 38 401 e-110 >Q9AJR6.2 Q9AJR6_BACGL Alpha-D-mannosidase Length = 1047 Score = 448 bits (1153), Expect = e-124, Method: Compositional matrix adjust. Identities = 216/346 (62%), Positives = 256/346 (73%), Gaps = 2/346 (0%) Query: 48 FEWEDSRLTTPFYVLEWNDAGQLSRIYDRREQREVLAPGENGNVLQVFEDKPKMYDAWDI 107 FEW D L TP Y L +++AG+L R+YDR REVL G N LQVFEDKPKM++AWDI Sbjct: 702 FEWADGVLRTPHYELAFDEAGRLVRLYDRGLSREVLPRGARANELQVFEDKPKMFEAWDI 761 Query: 108 DLFYQEKKREITDLRSVKLTECGALAAVLEFTWQYMDSSIVQKVKVYSRSARIDFETLAD 167 DLFYQEK+RE+TDLR ++L ECGAL AVL W Y S I Q++ VY+ RIDF T D Sbjct: 762 DLFYQEKRREVTDLRKLELVECGALRAVLRLEWAYGASVIEQRLVVYADDRRIDFVTRVD 821 Query: 168 WHEQHQLLKTAFPVAVRATEATYDIQFGNVKRPTHWNTSWDYARFESVGHQWADLSERGY 227 W E QLLK AFPV +RATEATYDIQFGNVKRPTHWNTSWD+ARFE+VGHQW DLSERGY Sbjct: 822 WREHQQLLKVAFPVEIRATEATYDIQFGNVKRPTHWNTSWDHARFETVGHQWVDLSERGY 881 Query: 228 GVSLLNDCKYGYDIKDHVMRLSLIKSATEPDWQADQGEHRFTYALLPHKGDWVAANTVQE 287 GVSLLNDCKYG+DIKD+V+RLSLIKSA +PD ADQGEH FTY+L PH GDW + T Sbjct: 882 GVSLLNDCKYGHDIKDNVIRLSLIKSAIDPDPNADQGEHLFTYSLYPHAGDWASGGTATA 941 Query: 288 AWSLNNPLRVVKGGYSAGAGRSLIKTDCGSIAVDALKMAENGEGFIVRLHEYTGTRSTVT 347 AW+LNNPL V G SA AG+SL++ D IA DA+K AE+ + +VRLHEY G R T Sbjct: 942 AWALNNPLLAVPGA-SAFAGKSLLRADGAHIAFDAIKKAEDEDALVVRLHEYAGGRGLTT 1000 Query: 348 FSSDYPVHSWQECDLMERPVGEIKPGGA-VTCEIKPYEISTFIVRF 392 + + SWQECDL+ERP+G+ G + E+ PYEI TF++R Sbjct: 1001 LTPGCEIASWQECDLLERPIGDRHDGDKPLRFELAPYEIKTFLIRL 1046 >A4IPC6.1 A4IPC6_GEOTN Alpha-D-mannosidase Length = 1044 Score = 442 bits (1136), Expect = e-122, Method: Compositional matrix adjust. Identities = 213/354 (60%), Positives = 258/354 (72%), Gaps = 3/354 (0%) Query: 37 GNALTPGDPVPFEWEDSRLTTPFYVLEWNDAGQLSRIYDRREQREVLAPGENGNVLQVFE 96 GN L D F S +T+P Y ++WND G+++ IYD +REVL G+ GNVLQVFE Sbjct: 691 GNTLAQTDL--FHIGSSEMTSPHYKIKWNDKGRITSIYDFEAKREVLDDGKCGNVLQVFE 748 Query: 97 DKPKMYDAWDIDLFYQEKKREITDLRSVKLTECGALAAVLEFTWQYMDSSIVQKVKVYSR 156 DKP +DAWDID+FYQEKKRE+ DLR V + E L A++ F W YMDSSI Q + VY+ Sbjct: 749 DKPLRFDAWDIDIFYQEKKREVEDLRRVSVKEITPLYAIVHFEWGYMDSSIKQDMIVYAH 808 Query: 157 SARIDFETLADWHEQHQLLKTAFPVAVRATEATYDIQFGNVKRPTHWNTSWDYARFESVG 216 S RIDF+T DW EQ+QLLK AFPV +R+T ATYDIQFGNVKRPTHWNTSWDYA+FE+V Sbjct: 809 SRRIDFKTEVDWKEQNQLLKVAFPVKIRSTLATYDIQFGNVKRPTHWNTSWDYAKFETVA 868 Query: 217 HQWADLSERGYGVSLLNDCKYGYDIKDHVMRLSLIKSATEPDWQADQGEHRFTYALLPHK 276 HQWADLSE GYGVSLLNDCKYGYDIKD+VMRLSLIKSAT PD++ DQG H FTY+LLPH Sbjct: 869 HQWADLSETGYGVSLLNDCKYGYDIKDNVMRLSLIKSATYPDYKQDQGHHIFTYSLLPHV 928 Query: 277 GDWVAANTVQEAWSLNNPLRVVKGGYSAGAGRSLIKTDCGSIAVDALKMAENGEGFIVRL 336 GDWV TV+EAW LNNPL + G SL + D +I +DA+K AE+ ++RL Sbjct: 929 GDWVEGQTVKEAWYLNNPL-TYRIGQPVKETFSLFRVDANNIMIDAVKKAEDTNQIVLRL 987 Query: 337 HEYTGTRSTVTFSSDYPVHSWQECDLMERPVGEIKPGGAVTCEIKPYEISTFIV 390 HE+TG R V +SD + +WQECDLMER +GE K + +I PYEI TF++ Sbjct: 988 HEFTGRRGKVCITSDLSITAWQECDLMERGIGEKKTDATLEFDINPYEIKTFLI 1041 >B4BM85.1 B4BM85_9BACI Alpha-mannosidase EC=3.2.1.24 Length = 1044 Score = 441 bits (1133), Expect = e-122, Method: Compositional matrix adjust. Identities = 213/354 (60%), Positives = 257/354 (72%), Gaps = 3/354 (0%) Query: 37 GNALTPGDPVPFEWEDSRLTTPFYVLEWNDAGQLSRIYDRREQREVLAPGENGNVLQVFE 96 GN L D F S +T+P Y ++WND GQ++ IYD +REVL G+ GNVLQVFE Sbjct: 691 GNTLAQTDL--FHIGSSEMTSPHYKIKWNDKGQITSIYDFEAKREVLDDGKCGNVLQVFE 748 Query: 97 DKPKMYDAWDIDLFYQEKKREITDLRSVKLTECGALAAVLEFTWQYMDSSIVQKVKVYSR 156 DKP +DAWDID+FYQEKKRE+ DLR V + E L A++ F W YMDSSI Q + VY+ Sbjct: 749 DKPLRFDAWDIDIFYQEKKREVEDLRRVSVKEITPLYAIVHFEWGYMDSSIKQDMIVYAH 808 Query: 157 SARIDFETLADWHEQHQLLKTAFPVAVRATEATYDIQFGNVKRPTHWNTSWDYARFESVG 216 S RIDF+T DW EQ+QLLK AFPV +R+T ATYDIQFGNVKRPTHWNTSWDYA+FE+V Sbjct: 809 SRRIDFKTEVDWKEQNQLLKVAFPVKIRSTLATYDIQFGNVKRPTHWNTSWDYAKFETVA 868 Query: 217 HQWADLSERGYGVSLLNDCKYGYDIKDHVMRLSLIKSATEPDWQADQGEHRFTYALLPHK 276 HQWADLSE GYGVSLLNDCKYGYDIKD+VMRLSLIKSAT PD++ DQG H FTY+LLPH Sbjct: 869 HQWADLSETGYGVSLLNDCKYGYDIKDNVMRLSLIKSATYPDYKQDQGHHIFTYSLLPHV 928 Query: 277 GDWVAANTVQEAWSLNNPLRVVKGGYSAGAGRSLIKTDCGSIAVDALKMAENGEGFIVRL 336 GDWV TV+EAW LNNPL + G SL + D +I +DA+K AE+ ++RL Sbjct: 929 GDWVEGQTVKEAWYLNNPL-TYRIGQPVKETFSLFRVDANNIMIDAVKKAEDTNQIVLRL 987 Query: 337 HEYTGTRSTVTFSSDYPVHSWQECDLMERPVGEIKPGGAVTCEIKPYEISTFIV 390 HE+TG R V +SD + +WQECDLMER +G K + +I PYEI TF++ Sbjct: 988 HEFTGRRGKVCITSDLSITAWQECDLMERGIGGKKTDAKLEFDINPYEIKTFLI 1041 >Q5KYP9.1 Q5KYP9_GEOKA Alpha-D-mannosidase EC=3.2.1.24 Length = 1044 Score = 438 bits (1127), Expect = e-121, Method: Compositional matrix adjust. Identities = 208/338 (61%), Positives = 255/338 (75%), Gaps = 1/338 (0%) Query: 53 SRLTTPFYVLEWNDAGQLSRIYDRREQREVLAPGENGNVLQVFEDKPKMYDAWDIDLFYQ 112 S + +P+Y ++WND GQ+ IYD + +REVL G+ GNVLQVFEDKP +DAWDID+FYQ Sbjct: 705 SEVISPYYKIKWNDKGQIISIYDLQAKREVLDDGKCGNVLQVFEDKPLRFDAWDIDIFYQ 764 Query: 113 EKKREITDLRSVKLTECGALAAVLEFTWQYMDSSIVQKVKVYSRSARIDFETLADWHEQH 172 EKKRE+ DL+ V + E +L AV+ F W+YMDS I Q + +Y+ S RIDF+T DW EQ+ Sbjct: 765 EKKREVEDLQHVSIKEITSLYAVIHFEWKYMDSIIKQDMILYTHSRRIDFKTEVDWKEQN 824 Query: 173 QLLKTAFPVAVRATEATYDIQFGNVKRPTHWNTSWDYARFESVGHQWADLSERGYGVSLL 232 QLLK AFPV +R+T ATYDIQFGNVKRPTHWNTSWDYA+FE+V HQWADLSE GYGVSLL Sbjct: 825 QLLKVAFPVKIRSTLATYDIQFGNVKRPTHWNTSWDYAKFETVAHQWADLSETGYGVSLL 884 Query: 233 NDCKYGYDIKDHVMRLSLIKSATEPDWQADQGEHRFTYALLPHKGDWVAANTVQEAWSLN 292 NDCKYGYDIKD+V+RLSLIKSAT PD+Q DQG H FTY+LLPH+GDWV NTV+EAW LN Sbjct: 885 NDCKYGYDIKDNVIRLSLIKSATYPDYQQDQGYHIFTYSLLPHQGDWVEGNTVKEAWYLN 944 Query: 293 NPLRVVKGGYSAGAGRSLIKTDCGSIAVDALKMAENGEGFIVRLHEYTGTRSTVTFSSDY 352 NPL G SL T+ +I VD +K AE+ ++RLHE+TG R+ V +SD Sbjct: 945 NPLTYAVGE-PIRKKYSLFGTNTDNIMVDVVKRAEDSNRIVLRLHEFTGRRNKVYITSDL 1003 Query: 353 PVHSWQECDLMERPVGEIKPGGAVTCEIKPYEISTFIV 390 P+ +WQECDLMER VGE + +I PYEI TF++ Sbjct: 1004 PIFAWQECDLMEREVGEGTISEKLEFDINPYEIKTFLI 1041 >A9KS60.1 A9KS60_CLOPH Glycoside hydrolase family 38 Length = 1043 Score = 401 bits (1031), Expect = e-110, Method: Compositional matrix adjust. Identities = 180/346 (52%), Positives = 245/346 (70%) Query: 46 VPFEWEDSRLTTPFYVLEWNDAGQLSRIYDRREQREVLAPGENGNVLQVFEDKPKMYDAW 105 +PFE+ + + TPFY+++ N GQ++ IYD+ +R+V+APGE NVLQVFEDKP DAW Sbjct: 695 LPFEYSNHCIETPFYLIQLNKEGQITSIYDKENKRQVIAPGERANVLQVFEDKPMDNDAW 754 Query: 106 DIDLFYQEKKREITDLRSVKLTECGALAAVLEFTWQYMDSSIVQKVKVYSRSARIDFETL 165 DID+FYQEK RE++DL ++ E G L AV F W+YM+S I Q + +Y+ S RIDFET Sbjct: 755 DIDIFYQEKMREVSDLEHFEIKEIGTLRAVFSFQWKYMNSVIKQDMTLYAYSRRIDFETY 814 Query: 166 ADWHEQHQLLKTAFPVAVRATEATYDIQFGNVKRPTHWNTSWDYARFESVGHQWADLSER 225 D+HE+ QLLK AFPV +R+T ATYDIQ+GNVKRP HWNTSWD A+FE+V H+W DLSE Sbjct: 815 VDYHEKRQLLKVAFPVDIRSTYATYDIQYGNVKRPNHWNTSWDQAKFETVAHKWVDLSEY 874 Query: 226 GYGVSLLNDCKYGYDIKDHVMRLSLIKSATEPDWQADQGEHRFTYALLPHKGDWVAANTV 285 YGVSLLN+CKYG+D+KD+++RL+LIK+A PD+ DQGEH FTY+LLPHKGD+V T Sbjct: 875 NYGVSLLNNCKYGHDVKDNLLRLTLIKTAMHPDYLQDQGEHSFTYSLLPHKGDFVKGETE 934 Query: 286 QEAWSLNNPLRVVKGGYSAGAGRSLIKTDCGSIAVDALKMAENGEGFIVRLHEYTGTRST 345 +EA++LN L + G S + D I +DA+K +E+ E +VR H+Y+G+++ Sbjct: 935 KEAFALNQKLEAIAGECVLPNNYSFVSIDNEYITIDAMKQSEDKEYLVVRFHDYSGSKNI 994 Query: 346 VTFSSDYPVHSWQECDLMERPVGEIKPGGAVTCEIKPYEISTFIVR 391 V + + V W E DL ERP+ + + E+KPYEI T + + Sbjct: 995 VKLQTGFKVTEWVEADLRERPMDDTHYDSDIILEVKPYEIKTILAK 1040 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.134 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,399,135,899 Number of extensions: 99251124 Number of successful extensions: 203454 Number of sequences better than 1.0e-05: 313 Number of HSP's gapped: 202828 Number of HSP's successfully gapped: 313 Length of query: 394 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 256 Effective length of database: 1,760,596,133 Effective search space: 450712610048 Effective search space used: 450712610048 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)