BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0346.0009.p (473 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q3Y3F7.1 Q3Y3F7_ENTFC Mannitol dehydrogenase 542 e-152 C2H7R3.1 C2H7R3_ENTFC Tagaturonate reductase EC=1.1.1.58 536 e-150 C1P816.1 C1P816_BACCO Mannitol dehydrogenase domain protein 491 e-137 A6LUH9.1 A6LUH9_CLOB8 Mannitol dehydrogenase, C-terminal domain 484 e-135 A6TKR4.1 A6TKR4_ALKMQ Mannitol dehydrogenase, C-terminal domain 482 e-134 >Q3Y3F7.1 Q3Y3F7_ENTFC Mannitol dehydrogenase Length = 473 Score = 542 bits (1397), Expect = e-152, Method: Compositional matrix adjust. Identities = 258/452 (57%), Positives = 335/452 (74%) Query: 17 PERMIQFGEGNFMRAFVDWQLQQMNQQGLFNGSAAVVVPIGQGIGGLMAEQDNLYTVLLN 76 P +++QFGEGNFMR F+DWQ+QQ+N Q LF G+ A++ P+ QG+G +M +QDNLYTV+L Sbjct: 15 PVKVLQFGEGNFMRGFIDWQIQQLNNQQLFKGNVAIIQPLEQGLGNMMNQQDNLYTVILQ 74 Query: 77 GIMQEQPVNSREIITSVSRVINPYSDYEAYLALAENDELEFITSNTTEAGIAYQPEDRLE 136 G+ + V++ EIIT V ++INPY +++AYL+LAEND+L +I SNTTEAGI + D L Sbjct: 75 GLSNGEIVDTSEIITVVEQMINPYDEWDAYLSLAENDDLAYIVSNTTEAGIQFVETDNLT 134 Query: 137 DAPPSSFPAKLTALLYRRFELGKKGFVIIPCELIDRNGEKLKEIVRQYAAAWNLGEAFLQ 196 D PP SFP KLTA LYRRF+L K GF IIPCELIDRNGE+LKEI+ +YA W+L AF++ Sbjct: 135 DTPPQSFPGKLTAFLYRRFKLNKAGFTIIPCELIDRNGERLKEIILRYAEKWDLEPAFVE 194 Query: 197 WLDDENTFCCSLVDRIVPGYPRGKAAELEAELGYLDKLMVTAEPFLFWVIEGPAWLSERL 256 WL+ EN FCCSLVDRIVPGYPR A Y D+LMV AEPF+ WVIEGP L +L Sbjct: 195 WLEKENVFCCSLVDRIVPGYPREDAGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQL 254 Query: 257 PLAKAGLNVVVTDDMTLYRERKVHLLNGPHTAMVPLAMMAGLETVEDVMNDETFYRFVRR 316 P +AGLNV++TDDMT YRERKVHLLNGPHTAMVPLA++A LETVE+VM D F +V + Sbjct: 255 PFKQAGLNVIITDDMTPYRERKVHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQ 314 Query: 317 LLDEELIPVLDLPQEELLSYADAVLERFKNPFIRHELASISLNSISKFKTRLLPVLLRYQ 376 L + ELIP+L LP++EL YAD + ERF NPF H+L +ISLNS+SKF TRLLPV +Y Sbjct: 315 LFNLELIPMLSLPKDELAIYADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYI 374 Query: 377 QERGQLPPLITLAFAGLLLSYRGDRVKRQDSAEVLAVFDQAWSQPSTFTASILREVSLWG 436 +E+ Q+PPLIT++ A LLL YRGD+VK D + ++ F AWS T +L+ +LWG Sbjct: 375 EEQNQVPPLITVSLAALLLMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWG 434 Query: 437 QDLTQVPGLTEAVDARLHQLQSSDSRAALQQL 468 +DL+Q+P +T+ V ++ R+ LQ++ Sbjct: 435 EDLSQIPNVTDTVQEIAAKIDQKGVRSVLQEM 466 >C2H7R3.1 C2H7R3_ENTFC Tagaturonate reductase EC=1.1.1.58 Length = 473 Score = 536 bits (1380), Expect = e-150, Method: Compositional matrix adjust. Identities = 258/452 (57%), Positives = 333/452 (73%) Query: 17 PERMIQFGEGNFMRAFVDWQLQQMNQQGLFNGSAAVVVPIGQGIGGLMAEQDNLYTVLLN 76 P +++QFGEGNFMR FVDWQ+QQ+N Q LF G+ A++ P+ QG+G +M +QDNLYTV+L Sbjct: 15 PVKVLQFGEGNFMRGFVDWQIQQLNNQQLFKGNVAIIQPLEQGLGNMMNQQDNLYTVILQ 74 Query: 77 GIMQEQPVNSREIITSVSRVINPYSDYEAYLALAENDELEFITSNTTEAGIAYQPEDRLE 136 G+ + V++ EIIT V ++INPY +++AYL+LAEND+L +I SNTTEAGI + D Sbjct: 75 GLSNGEIVDTSEIITIVEQMINPYHEWDAYLSLAENDDLAYIVSNTTEAGIQFVETDERT 134 Query: 137 DAPPSSFPAKLTALLYRRFELGKKGFVIIPCELIDRNGEKLKEIVRQYAAAWNLGEAFLQ 196 D PP SFP+KLTA LYRRFEL K GF IIPCELIDRNGE+LKEI+ +YA W+L AF++ Sbjct: 135 DTPPQSFPSKLTAFLYRRFELNKAGFTIIPCELIDRNGERLKEIILRYAEKWDLEPAFIE 194 Query: 197 WLDDENTFCCSLVDRIVPGYPRGKAAELEAELGYLDKLMVTAEPFLFWVIEGPAWLSERL 256 WL+ EN FCCSLVDRIVPGYPR A Y D+LMV AEPF+ WVIEGP L +L Sbjct: 195 WLEKENVFCCSLVDRIVPGYPREDADVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQL 254 Query: 257 PLAKAGLNVVVTDDMTLYRERKVHLLNGPHTAMVPLAMMAGLETVEDVMNDETFYRFVRR 316 +AGLNV++TDDMT YRERKVHLLNGPHTAMVPLA++A LETVE+VM D F +V + Sbjct: 255 RFKQAGLNVIITDDMTPYRERKVHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQ 314 Query: 317 LLDEELIPVLDLPQEELLSYADAVLERFKNPFIRHELASISLNSISKFKTRLLPVLLRYQ 376 L D ELIP+L LP++EL YAD + ERF NPF H+L +ISLNS+SKF TRLLPV +Y Sbjct: 315 LFDLELIPMLSLPKDELAIYADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYI 374 Query: 377 QERGQLPPLITLAFAGLLLSYRGDRVKRQDSAEVLAVFDQAWSQPSTFTASILREVSLWG 436 +E+ Q+PPLI+++ A LLL YRGD+VK D + ++ F AWS T +L+ +LWG Sbjct: 375 EEKNQVPPLISVSLAALLLMYRGDQVKPYDDEKTISEFVDAWSDNETAILRLLQNAALWG 434 Query: 437 QDLTQVPGLTEAVDARLHQLQSSDSRAALQQL 468 +DL+Q+P + + V ++ RA LQ++ Sbjct: 435 EDLSQLPNVADTVQEMATKIDQKGVRAVLQEM 466 >C1P816.1 C1P816_BACCO Mannitol dehydrogenase domain protein Length = 478 Score = 491 bits (1264), Expect = e-137, Method: Compositional matrix adjust. Identities = 249/469 (53%), Positives = 319/469 (68%), Gaps = 6/469 (1%) Query: 3 QRLSRTTQPGLPQYPERMIQFGEGNFMRAFVDWQLQQMNQQGLFNGSAAVVVPIGQGIGG 62 ++LS + L +YP + IQFGEGNF+RAFVDWQ++QMN++GLF G +V + +G G Sbjct: 2 KKLSYSYVKDLQRYPTKAIQFGEGNFLRAFVDWQIEQMNKRGLFQGGVTIVQSLPRGRVG 61 Query: 63 LMAEQDNLYTVLLNGIMQEQPVNSREIITSVSRVINPYSDYEAYLALAENDELEFITSNT 122 +A QD L+TV+L G+ + V S EII+SV+ INPY DY+AYL LA +D + FI SNT Sbjct: 62 QLAGQDFLFTVMLEGMQNGKAVQSHEIISSVNSGINPYEDYDAYLQLAHDDNIRFIFSNT 121 Query: 123 TEAGIAYQPEDRLEDAPPSSFPAKLTALLYRRFELGKKGFVIIPCELIDRNGEKLKEIVR 182 TEAGI Y+ D L D PP SFPAKLTALLY R++ GKKGF IIPCELI+ NG+ LKEIV Sbjct: 122 TEAGIVYREGDALTDRPPVSFPAKLTALLYERYKAGKKGFYIIPCELIENNGKALKEIVH 181 Query: 183 QYAAAWNLGEAFLQWLDDENTFCCSLVDRIVPGYPRGKAAELEAELGYLDKLMVTAEPFL 242 +YA W LGE F WL+ ENTFC SLVDRIVPGYP+ +A +L E GY D L+ AEPFL Sbjct: 182 RYADEWKLGEDFSSWLESENTFCSSLVDRIVPGYPKDRAEKLWEEWGYEDTLIAKAEPFL 241 Query: 243 FWVIEGPAWLSERLPLAKAGLNVVVTDDMTLYRERKVHLLNGPHTAMVPLAMMAGLETVE 302 WVIEGP L E LPL K GLNVVVTD + YR+RKV+LLN PHT M LA + G++TV Sbjct: 242 IWVIEGPKELEEELPLKKVGLNVVVTDHLKPYRDRKVYLLNAPHTTMACLARLVGIKTVG 301 Query: 303 DVMNDETFYRFVRRLLDEELIPVLDLPQEELLSYADAVLERFKNPFIRHELASISLNSIS 362 VM D FYR + R + E+IP LDLP+EEL SYA + ERF+NP++ H+L +I+LNS+S Sbjct: 302 GVMEDPDFYRLIDREMFAEIIPSLDLPKEELTSYAAGIQERFRNPYMEHQLDAIALNSVS 361 Query: 363 KFKTRLLPVLLRYQQERGQLPPLITLAFAGLLLSYRGDR----VKRQDSAEVLAVFDQAW 418 KFK RLLP+L + + ++P I LA +GLL Y G++ K D +VL F A+ Sbjct: 362 KFKARLLPILAEHVKRFHKIPSRIALALSGLLKIY-GEQNPASAKAIDLPDVLKEFSMAY 420 Query: 419 SQPSTFTASILREVSLWGQDLTQVPGLTEAVDARLHQLQSSDSRAALQQ 467 + IL +LWG DLT++ GL V+ L ++ R A+QQ Sbjct: 421 -HSDRYVWDILSNETLWGMDLTKIDGLPAQVEKGLQLIEKEGVRRAVQQ 468 >A6LUH9.1 A6LUH9_CLOB8 Mannitol dehydrogenase, C-terminal domain Length = 482 Score = 484 bits (1245), Expect = e-135, Method: Compositional matrix adjust. Identities = 230/481 (47%), Positives = 321/481 (66%), Gaps = 15/481 (3%) Query: 4 RLSRTTQPGLPQYPERMIQFGEGNFMRAFVDWQLQQMNQQGLFNGSAAVVVPIGQGIGGL 63 +L++ YPE+++QFGEGNF+RAFVDWQ+ +MN++ FNGS VV P G+ + Sbjct: 2 KLNKKIYKEFKTYPEKVLQFGEGNFLRAFVDWQIDKMNEEAGFNGSVVVVQPQEGGLVNM 61 Query: 64 MAEQDNLYTVLLNGIMQEQPVNSREIITSVSRVINPYSDYEAYLALAENDELEFITSNTT 123 + EQ+ L+T+ L G+ ++ + + +II S+SR INPY+DY YL +AEN EL FI SNTT Sbjct: 62 LNEQNGLFTLYLQGVQGKRAIKTHKIINSISRGINPYTDYNEYLRVAENPELRFIVSNTT 121 Query: 124 EAGIAYQPEDRLEDAPPSSFPAKLTALLYRRFEL----GKKGFVIIPCELIDRNGEKLKE 179 EAGIA+ D+L + SFP KLTA L+RRF + KGF+IIPCELIDRNGEKLKE Sbjct: 122 EAGIAFDENDKLNEGCQKSFPGKLTAFLFRRFMVFNGDNSKGFIIIPCELIDRNGEKLKE 181 Query: 180 IVRQYAAAWNLGEAFLQWLDDENTFCCSLVDRIVPGYPRGKAAELEAELGYLDKLMVTAE 239 IV +YA WNLG+ F+ W++D NTFCCSLVDRIVPGYPR E+ ELGY D L+ E Sbjct: 182 IVLRYAEMWNLGQDFVNWINDANTFCCSLVDRIVPGYPRDTIDEVREELGYDDNLVDVGE 241 Query: 240 PFLFWVIEGPAWLSERLPLAKAGLNVVVTDDMTLYRERKVHLLNGPHTAMVPLAMMAGLE 299 F WVIEGP + + LP+ KAGLNV V DDMT YR RKV +LNGPHTAMVP+A + GLE Sbjct: 242 IFHLWVIEGPQSIKDELPIEKAGLNVKVVDDMTPYRTRKVRILNGPHTAMVPVAYLYGLE 301 Query: 300 TVEDVMNDETFYRFVRRLLDEELIPVLDLPQEELLSYADAVLERFKNPFIRHELASISLN 359 TV + ++ E R+V ++ +E+I LDLP EEL+ +ADA++ERF+NP+++H L SI+LN Sbjct: 302 TVGEAVDHEVIGRYVHDVIYDEIIETLDLPHEELVEFADAIIERFQNPYVKHYLMSIALN 361 Query: 360 SISKFKTRLLPVLLRYQQERGQLPPLITLAFAGLLLSYRGDR----VKRQDSAEVLAVFD 415 S+SK+KTR LP L Y + +G LP + + A L+ Y+G R ++ D ++L +F Sbjct: 362 SLSKYKTRNLPSLTEYLKRKGTLPKKLVFSLASLIEFYKGKRGDEDIQLVDDEDILELFK 421 Query: 416 QAWSQP-------STFTASILREVSLWGQDLTQVPGLTEAVDARLHQLQSSDSRAALQQL 468 + W + + S+L WG +L ++P L + V L ++ + A++++ Sbjct: 422 ELWGKYDGTKEGLNKIVTSVLAYEKNWGSNLNEIPNLADEVSRYLEIIEKVGMKEAIKEV 481 Query: 469 V 469 + Sbjct: 482 I 482 >A6TKR4.1 A6TKR4_ALKMQ Mannitol dehydrogenase, C-terminal domain Length = 483 Score = 482 bits (1241), Expect = e-134, Method: Compositional matrix adjust. Identities = 232/482 (48%), Positives = 318/482 (65%), Gaps = 15/482 (3%) Query: 4 RLSRTTQPGLPQYPERMIQFGEGNFMRAFVDWQLQQMNQQGLFNGSAAVVVPIGQGIGGL 63 +L++T YPE+++QFGEGNF+RAF DW + +MN++ FN V+ P+ G+ Sbjct: 2 KLNKTIYKEARNYPEKIVQFGEGNFLRAFADWTIDKMNKEANFNSGVVVIQPLQGGLIDK 61 Query: 64 MAEQDNLYTVLLNGIMQEQPVNSREIITSVSRVINPYSDYEAYLALAENDELEFITSNTT 123 + +QD LYT+ LNGI + V+ +I V+R IN Y++Y+ YLA+AEN EL F+ SNTT Sbjct: 62 LNDQDGLYTLYLNGIKDGEVVSEHAVIDCVTRGINTYTNYDEYLAVAENPELRFMISNTT 121 Query: 124 EAGIAYQPEDRLEDAPPSSFPAKLTALLYRRFEL----GKKGFVIIPCELIDRNGEKLKE 179 EAGI Y +DRLED P +SFP KLTALLY RF+ KG V IPCELI++NGEKLKE Sbjct: 122 EAGITYNEQDRLEDRPQASFPGKLTALLYHRFKTFNGSKDKGLVFIPCELIEKNGEKLKE 181 Query: 180 IVRQYAAAWNLGEAFLQWLDDENTFCCSLVDRIVPGYPRGKAAELEAELGYLDKLMVTAE 239 V + AA WNL F W+D+ NTFC SLVDRIVPGYP+ + E+ ELGY D L+V E Sbjct: 182 TVLKLAALWNLENDFKVWIDEANTFCNSLVDRIVPGYPKERMEEIRQELGYDDDLVVEGE 241 Query: 240 PFLFWVIEGPAWLSERLPLAKAGLNVVVTDDMTLYRERKVHLLNGPHTAMVPLAMMAGLE 299 PF WVIEGPAW+ E P K GLNV+V DDMT YR RKV +LNG HT+MVP+A + G++ Sbjct: 242 PFHLWVIEGPAWVKEAFPAHKVGLNVLVVDDMTPYRTRKVRILNGAHTSMVPVAYLYGID 301 Query: 300 TVEDVMNDETFYRFVRRLLDEELIPVLDLPQEELLSYADAVLERFKNPFIRHELASISLN 359 TV + + D +F++ + EE+IP LDLP+EEL +A+ VL+RF+NPFI+H+L SISLN Sbjct: 302 TVRESVEDSVVGQFIKETIFEEIIPTLDLPREELDGFANDVLDRFRNPFIKHQLMSISLN 361 Query: 360 SISKFKTRLLPVLLRYQQERGQLPPLITLAFAGLLLSYRGDR----VKRQDSAEVLAVFD 415 S+SKF+TR+LP +L Y+ +G+LP +T + A L+ Y+G+R + D A +L + Sbjct: 362 SMSKFETRVLPSILEYENRKGELPKKLTFSLAALIAFYKGERNGEKIALTDDAAILEQYK 421 Query: 416 QAWS-------QPSTFTASILREVSLWGQDLTQVPGLTEAVDARLHQLQSSDSRAALQQL 468 W Q +IL +W DL Q+ GLT+ V L ++Q + LQ++ Sbjct: 422 TLWKAYEGKEDQLKHIVTTILSNEKIWKMDLNQISGLTDMVTNYLSKIQEHGVKNVLQRM 481 Query: 469 VR 470 ++ Sbjct: 482 MK 483 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.136 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,793,129,373 Number of extensions: 119093159 Number of successful extensions: 290692 Number of sequences better than 1.0e-05: 1212 Number of HSP's gapped: 287863 Number of HSP's successfully gapped: 1212 Length of query: 473 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 333 Effective length of database: 1,741,754,103 Effective search space: 580004116299 Effective search space used: 580004116299 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)