BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0346.0001.p (375 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DMK5.1 B1DMK5_9BACL Putative DNA alkylation repair enzyme 523 e-146 A6LYP2.1 A6LYP2_CLOB8 Putative DNA alkylation repair enzyme 452 e-125 C2L332.1 C2L332_LACSK DNA alkylation repair enzyme 395 e-108 Q38XG8.1 Q38XG8_LACSS Putative DNA alkylation repair enzyme 395 e-108 C0C5U2.1 C0C5U2_9CLOT Putative uncharacterized protein 375 e-102 >B1DMK5.1 B1DMK5_9BACL Putative DNA alkylation repair enzyme Length = 371 Score = 523 bits (1346), Expect = e-146, Method: Compositional matrix adjust. Identities = 243/368 (66%), Positives = 291/368 (79%) Query: 1 MAEPLKAMYNEGFLRSFGTKLQSVYSGFDLEGFVTAAMKDPWEELELKGRMRRITETLGT 60 MAEPLK MY+E FLR F K+ + Y F E FV+ M + W EL LKGR+R+I+ LG Sbjct: 1 MAEPLKDMYDEAFLRQFADKVLAAYPAFAKEAFVSDVMNEQWNELALKGRIRQISTVLGR 60 Query: 61 FLPRRYEDALEILFAIDKECVGFPYLIFPDFVEVFGGSEEHWELSMSALERFTPKSSAEF 120 LP Y DAL +L+AI +EC GFPYL FPDFVEV+GG+EEHWELSM ALERFTPKSSAEF Sbjct: 61 HLPANYGDALAVLYAICEECSGFPYLFFPDFVEVYGGAEEHWELSMQALERFTPKSSAEF 120 Query: 121 AVRPFLLRDPQRMMAQMLRWSQHPDEHVRRLSSEGSRPRLPWGQALPMFKHDPSPVLPVL 180 AVR FLLRDP+RMM QML W+ P+EHVRRL+SEG RPRLPWGQALP+FK +P+P+LPVL Sbjct: 121 AVRVFLLRDPERMMKQMLTWASDPNEHVRRLASEGCRPRLPWGQALPVFKREPAPLLPVL 180 Query: 181 EQLKADSSLYVRKSVANNLNDIAKDHPGVVIDIVRRWKGVNAHTDWIVRHGCRTLIRKAN 240 E LK D SLYVRKSVANNLNDI KDHP VV+D+ RRW+G + +TDWI+RH CR L+R+A+ Sbjct: 181 ELLKEDPSLYVRKSVANNLNDITKDHPDVVLDLARRWQGQHPYTDWIIRHACRNLVRQAH 240 Query: 241 PEIMALFGYAGQEDESPLAVSAVLSVEPAELPIGGSCELAYALAVREGDEARIRIEYGID 300 PE +A+FGYA D +P+ SA + P+ L IG SC L Y + V+EG+ +RIEYGID Sbjct: 241 PEALAIFGYAMSVDSAPIVTSADIQARPSILEIGDSCRLEYEMQVQEGEPLHLRIEYGID 300 Query: 301 FVKAGGKTSRKLFLLSDKTVPGGTRLSGKRTHRWVDLTTRRHYAGEHKITLLVNGREAAS 360 FVK+GGKTSRKLFLLSDKTVPG +RLSG R HR+ DL+TRRHYAGEH+I L+VNG E AS Sbjct: 301 FVKSGGKTSRKLFLLSDKTVPGASRLSGSRVHRFADLSTRRHYAGEHRIVLMVNGVETAS 360 Query: 361 AVLVLKPQ 368 V+ L+PQ Sbjct: 361 TVIELRPQ 368 >A6LYP2.1 A6LYP2_CLOB8 Putative DNA alkylation repair enzyme Length = 373 Score = 452 bits (1163), Expect = e-125, Method: Compositional matrix adjust. Identities = 210/363 (57%), Positives = 263/363 (72%) Query: 1 MAEPLKAMYNEGFLRSFGTKLQSVYSGFDLEGFVTAAMKDPWEELELKGRMRRITETLGT 60 M+EPLK +Y FL F K+ +VY+ F+ FV + + PW+EL LK R+ RI E LG Sbjct: 1 MSEPLKNIYTLEFLNDFANKIHNVYAEFNKSDFVASVLAYPWDELPLKARIHRIAEILGN 60 Query: 61 FLPRRYEDALEILFAIDKECVGFPYLIFPDFVEVFGGSEEHWELSMSALERFTPKSSAEF 120 +LP +E+AL ILF+I+K CVGFPYL FPDFV +G E+++ELSM ALERFT +SS+EF Sbjct: 61 YLPNDFENALNILFSINKSCVGFPYLFFPDFVATYGNQEKNFELSMDALERFTQQSSSEF 120 Query: 121 AVRPFLLRDPQRMMAQMLRWSQHPDEHVRRLSSEGSRPRLPWGQALPMFKHDPSPVLPVL 180 A+RPFLL DP+ +M M++WS P+EHVRRLSSEG RPRLPWG ALPMFK DP PV VL Sbjct: 121 AIRPFLLSDPRGVMEYMMKWSLSPNEHVRRLSSEGCRPRLPWGIALPMFKADPLPVFKVL 180 Query: 181 EQLKADSSLYVRKSVANNLNDIAKDHPGVVIDIVRRWKGVNAHTDWIVRHGCRTLIRKAN 240 E LK D SLYVRKSVANNLNDI+KD+P V+ I W N +TDWI++ GCRTLI++AN Sbjct: 181 ENLKEDDSLYVRKSVANNLNDISKDNPDAVLAIAENWICHNQNTDWILKRGCRTLIKRAN 240 Query: 241 PEIMALFGYAGQEDESPLAVSAVLSVEPAELPIGGSCELAYALAVREGDEARIRIEYGID 300 P+ M+LFGY E+P+ +A +S +P +L IGGSCEL Y+L + G IR+EYGID Sbjct: 241 PKAMSLFGYTNSSAENPIFRNASISAQPNQLKIGGSCELNYSLDIGWGSPVHIRLEYGID 300 Query: 301 FVKAGGKTSRKLFLLSDKTVPGGTRLSGKRTHRWVDLTTRRHYAGEHKITLLVNGREAAS 360 F+K+ GK+SRKLF LSDKT+ L G R H + DLT R+HY G HKI L+VNG EAA Sbjct: 301 FIKSNGKSSRKLFFLSDKTLLPNAHLQGTRIHSFADLTVRKHYPGIHKIVLIVNGIEAAQ 360 Query: 361 AVL 363 L Sbjct: 361 TKL 363 >C2L332.1 C2L332_LACSK DNA alkylation repair enzyme Length = 378 Score = 395 bits (1016), Expect = e-108, Method: Compositional matrix adjust. Identities = 183/363 (50%), Positives = 243/363 (66%) Query: 1 MAEPLKAMYNEGFLRSFGTKLQSVYSGFDLEGFVTAAMKDPWEELELKGRMRRITETLGT 60 MAE LK +Y E FL +F ++Q VY F + FV A + D W +L LK R R+I LG Sbjct: 13 MAELLKDIYTEAFLTTFSQQVQEVYRPFKAQQFVRAVLADNWADLTLKQRTRQIAIVLGQ 72 Query: 61 FLPRRYEDALEILFAIDKECVGFPYLIFPDFVEVFGGSEEHWELSMSALERFTPKSSAEF 120 FLP+ Y +A+ +L + +C GF YL FPDFV V+G E W LSM ALE FT +SSAEF Sbjct: 73 FLPQDYSEAIALLEQLAPDCQGFAYLFFPDFVVVYGTRPEDWALSMHALEIFTQQSSAEF 132 Query: 121 AVRPFLLRDPQRMMAQMLRWSQHPDEHVRRLSSEGSRPRLPWGQALPMFKHDPSPVLPVL 180 A+R F+L DP+R M QM +W+Q + HVRRL+SEG RPRLPWG++LP+FK DP+PVL +L Sbjct: 133 AIRTFILTDPKRAMVQMTQWAQSANAHVRRLASEGCRPRLPWGESLPVFKQDPTPVLAIL 192 Query: 181 EQLKADSSLYVRKSVANNLNDIAKDHPGVVIDIVRRWKGVNAHTDWIVRHGCRTLIRKAN 240 E+LK D LYVR+SVANNLNDIAKDHP +VI+ ++W+G + DW++RHGCRTL+RK Sbjct: 193 ERLKNDPELYVRRSVANNLNDIAKDHPQLVIETAKKWQGESEAVDWVIRHGCRTLLRKNF 252 Query: 241 PEIMALFGYAGQEDESPLAVSAVLSVEPAELPIGGSCELAYALAVREGDEARIRIEYGID 300 + LFGYA +PL +SA + + IG Y + R + +R+EY +D Sbjct: 253 LTVQTLFGYAADSPANPLVLSATIKTTATTVAIGDKLAFHYQIRFRPEAKLLVRVEYAVD 312 Query: 301 FVKAGGKTSRKLFLLSDKTVPGGTRLSGKRTHRWVDLTTRRHYAGEHKITLLVNGREAAS 360 +VKA G+ SRKLF L++K + G LSG+R + DLTTR+HY G+H + L+VNGRE A Sbjct: 313 YVKANGQQSRKLFKLAEKEIDGRQGLSGERQINFKDLTTRKHYPGQHNVALIVNGREVAK 372 Query: 361 AVL 363 Sbjct: 373 TAF 375 >Q38XG8.1 Q38XG8_LACSS Putative DNA alkylation repair enzyme Length = 366 Score = 395 bits (1015), Expect = e-108, Method: Compositional matrix adjust. Identities = 183/363 (50%), Positives = 243/363 (66%) Query: 1 MAEPLKAMYNEGFLRSFGTKLQSVYSGFDLEGFVTAAMKDPWEELELKGRMRRITETLGT 60 MAE LK +Y E FL +F ++Q VY F + FV A + D W +L LK R R+I LG Sbjct: 1 MAELLKDIYTEAFLTTFSQQVQEVYRPFKAQQFVRAVLADNWADLTLKQRTRQIAIVLGQ 60 Query: 61 FLPRRYEDALEILFAIDKECVGFPYLIFPDFVEVFGGSEEHWELSMSALERFTPKSSAEF 120 FLP+ Y +A+ +L + +C GF YL FPDFV V+G E W LSM ALE FT +SSAEF Sbjct: 61 FLPQDYSEAIALLEQLAPDCQGFAYLFFPDFVVVYGTRPEDWALSMHALEIFTQQSSAEF 120 Query: 121 AVRPFLLRDPQRMMAQMLRWSQHPDEHVRRLSSEGSRPRLPWGQALPMFKHDPSPVLPVL 180 A+R F+L DP+R M QM +W+Q + HVRRL+SEG RPRLPWG++LP+FK DP+PVL +L Sbjct: 121 AIRTFILTDPKRAMVQMTQWAQSANAHVRRLASEGCRPRLPWGESLPVFKQDPTPVLAIL 180 Query: 181 EQLKADSSLYVRKSVANNLNDIAKDHPGVVIDIVRRWKGVNAHTDWIVRHGCRTLIRKAN 240 E+LK D LYVR+SVANNLNDIAKDHP +VI+ ++W+G + DW++RHGCRTL+RK Sbjct: 181 ERLKNDPELYVRRSVANNLNDIAKDHPQLVIETAKKWQGESEAVDWVIRHGCRTLLRKNF 240 Query: 241 PEIMALFGYAGQEDESPLAVSAVLSVEPAELPIGGSCELAYALAVREGDEARIRIEYGID 300 + LFGYA +PL +SA + + IG Y + R + +R+EY +D Sbjct: 241 LTVQTLFGYAADSPANPLVLSATIKTTATTVAIGDKLAFHYQIRFRPEAKLLVRVEYAVD 300 Query: 301 FVKAGGKTSRKLFLLSDKTVPGGTRLSGKRTHRWVDLTTRRHYAGEHKITLLVNGREAAS 360 +VKA G+ SRKLF L++K + G LSG+R + DLTTR+HY G+H + L+VNGRE A Sbjct: 301 YVKANGQQSRKLFKLAEKEIDGRQGLSGERQINFKDLTTRKHYPGQHNVALIVNGREVAK 360 Query: 361 AVL 363 Sbjct: 361 TAF 363 >C0C5U2.1 C0C5U2_9CLOT Putative uncharacterized protein Length = 368 Score = 375 bits (963), Expect = e-102, Method: Compositional matrix adjust. Identities = 187/375 (49%), Positives = 252/375 (67%), Gaps = 15/375 (4%) Query: 1 MAEPLKAMYNEGFLRSFGTKLQSVYSGFDLEGFVTAAMKDPWEELELKGRMRRITETLGT 60 M + LK +YN L+ +QS Y F + F+ M + W++LELK R+R+I+ +L Sbjct: 1 MPDLLKNVYNYNSLQKLAHTIQSAYPPFQADKFLQTTMDETWDKLELKARVRKISTSLAA 60 Query: 61 FLPRRYEDALEILFAIDKECV----GFPYLIFPDFVEVFGGSEEHWELSMSALERFTPKS 116 +LP Y AL IL DK GF + FPDF+EVFG EE+W+LSMSALER+T S Sbjct: 61 YLPEDYSRALAIL---DKAAAVFTNGFFGIFFPDFIEVFGQDEENWDLSMSALERYTQYS 117 Query: 117 SAEFAVRPFLLRDPQRMMAQMLRWSQHPDEHVRRLSSEGSRPRLPWGQALPMFKHDPSPV 176 ++EFAVRPF++ +RMMAQM WS H +EHVRRLSSEG RP LPWGQAL +K DPSPV Sbjct: 118 TSEFAVRPFIISHEERMMAQMYAWSGHENEHVRRLSSEGCRPLLPWGQALVKYKKDPSPV 177 Query: 177 LPVLEQLKADSSLYVRKSVANNLNDIAKDHPGVVIDIVRRWKGVNAHTDWIVRHGCRTLI 236 LP+LE+LKAD SLYVRKSVANNLNDI+K HP +VI + + W G N +TDWIV+HGCRTL+ Sbjct: 178 LPILERLKADPSLYVRKSVANNLNDISKTHPDLVISLAKEWYGKNEYTDWIVKHGCRTLL 237 Query: 237 RKANPEIMALFGYAGQEDESPLAVSAVLSVEPAELPIGGSCELAYALAVREGDEARIRIE 296 +K N EI+ALFGY D + + V ++EPA +P+G +++++ E A++R+E Sbjct: 238 KKGNREILALFGY---HDSASIKVDG-FTLEPAAVPMGEDITFSFSISTEEA--AKVRLE 291 Query: 297 YGIDFVKAGGKTSRKLFLLSDKTVPGGTRLSGKRTHRWVDLTTRRHYAGEHKITLLVNG- 355 Y +D++KA GK S K+F +S+ T+ + S + H + ++TTR+HY G H ITL+VNG Sbjct: 292 YAVDYMKANGKRSSKVFQISEITLGRNEKKSCTKKHSFAEMTTRKHYPGVHTITLIVNGA 351 Query: 356 -REAASAVLVLKPQR 369 RE L P R Sbjct: 352 KRERQDFELTAGPGR 366 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.137 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,298,918,442 Number of extensions: 96174434 Number of successful extensions: 198619 Number of sequences better than 1.0e-05: 206 Number of HSP's gapped: 198296 Number of HSP's successfully gapped: 206 Length of query: 375 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 238 Effective length of database: 1,770,017,148 Effective search space: 421264081224 Effective search space used: 421264081224 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)