BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0345.0035.p (305 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KLC5.1 B3KLC5_9BACI 6-phosphofructokinase EC=2.7.1.11 514 e-144 Q59214.1 K6PF_BACMC 6-phosphofructokinase EC=2.7.1.11 512 e-143 B1DL07.1 B1DL07_9BACL 6-phosphofructokinase EC=2.7.1.11 473 e-132 Q180P4.1 K6PF_CLOD6 6-phosphofructokinase EC=2.7.1.11 355 2e-96 Q0SW30.1 Q0SW30_CLOPS 6-phosphofructokinase EC=2.7.1.11 347 1e-93 >B3KLC5.1 B3KLC5_9BACI 6-phosphofructokinase EC=2.7.1.11 Length = 323 Score = 514 bits (1324), Expect = e-144, Method: Compositional matrix adjust. Identities = 252/305 (82%), Positives = 277/305 (90%) Query: 1 MNAAVRAVVRSAIYFGIEVFGIQRGYQGLLNRDIFPMDLRSVGDIIQRGGTILQSARCLE 60 MNAA+RAVVRS +Y+G+EV+GIQRGYQGLL DI MDLRSVGDIIQRGGTIL+SARC E Sbjct: 19 MNAALRAVVRSGLYYGLEVYGIQRGYQGLLENDIIKMDLRSVGDIIQRGGTILRSARCEE 78 Query: 61 FLTPEGQQKGADILNEMGIDGLVVCGGDGSYKGANKLSKLGINTMALPGTIDNDISFTDY 120 F T EGQQKGADILN+ GIDGLVV GGDGSY+GANKLSKLGI TM LPGTIDNDISFTDY Sbjct: 79 FKTAEGQQKGADILNQHGIDGLVVIGGDGSYQGANKLSKLGIKTMGLPGTIDNDISFTDY 138 Query: 121 TIGFDTAVGVVVDAINKLRDTMSSHERSSIVEVMGRHCGDIALHAGLASGAETILVPEMP 180 TIGFDTAV VVVDA+NKLRDTMSSH RSS+VEVMGRHCGDIALHAGLASGAETILVPE+ Sbjct: 139 TIGFDTAVSVVVDAVNKLRDTMSSHARSSVVEVMGRHCGDIALHAGLASGAETILVPEVE 198 Query: 181 YDLNEVADRMRDNFARGKRHSIVIVAEGVGKGEDVAQALKDRHSSLDARVTVLGHIQRGG 240 Y+L+EVA R+R+NFA+GKRHSI+IVAEGVG+GEDV LK+ H+S+DARVTVLGHIQRGG Sbjct: 199 YNLDEVATRLRENFAKGKRHSIIIVAEGVGRGEDVVHDLKECHASIDARVTVLGHIQRGG 258 Query: 241 TPTPGDRNLASRLGDFAVRSLIEGHSDKACGIIKGELTLTDIDLVVNTKKDFDTELYELA 300 PTP DRNLASRLGDFAVRSLI+G SDK CGIIKGEL LTDID VVNTKK+FD +LY+LA Sbjct: 259 APTPFDRNLASRLGDFAVRSLIDGQSDKGCGIIKGELVLTDIDKVVNTKKEFDRDLYDLA 318 Query: 301 SRLSQ 305 RLSQ Sbjct: 319 LRLSQ 323 >Q59214.1 K6PF_BACMC 6-phosphofructokinase EC=2.7.1.11 Length = 322 Score = 512 bits (1318), Expect = e-143, Method: Compositional matrix adjust. Identities = 246/305 (80%), Positives = 277/305 (90%) Query: 1 MNAAVRAVVRSAIYFGIEVFGIQRGYQGLLNRDIFPMDLRSVGDIIQRGGTILQSARCLE 60 MNAAVRAVVRS +++G+EV+GIQRGYQGLLN DIF MDLRSVGDIIQRGGT+LQSARC E Sbjct: 18 MNAAVRAVVRSGLFYGLEVYGIQRGYQGLLNDDIFSMDLRSVGDIIQRGGTVLQSARCKE 77 Query: 61 FLTPEGQQKGADILNEMGIDGLVVCGGDGSYKGANKLSKLGINTMALPGTIDNDISFTDY 120 F+TPEGQQKGADIL + GIDGLVV GGDGSY GANKLSKLGINTMALPGTIDNDIS+TD+ Sbjct: 78 FMTPEGQQKGADILRKRGIDGLVVIGGDGSYHGANKLSKLGINTMALPGTIDNDISYTDF 137 Query: 121 TIGFDTAVGVVVDAINKLRDTMSSHERSSIVEVMGRHCGDIALHAGLASGAETILVPEMP 180 TIGFDT+V +VVDAINKLRDTMSSHERSSIVEVMGRHCGDIAL+AGLASGAETI+VPE+P Sbjct: 138 TIGFDTSVSIVVDAINKLRDTMSSHERSSIVEVMGRHCGDIALYAGLASGAETIIVPEVP 197 Query: 181 YDLNEVADRMRDNFARGKRHSIVIVAEGVGKGEDVAQALKDRHSSLDARVTVLGHIQRGG 240 +D++E+A+RM+ NFA GKRHSIV+VAEG G GE+VA+ L +R +L+ RVTVLGHIQRGG Sbjct: 198 FDMDEIAERMKQNFAHGKRHSIVVVAEGAGNGENVAKQLVERCETLEPRVTVLGHIQRGG 257 Query: 241 TPTPGDRNLASRLGDFAVRSLIEGHSDKACGIIKGELTLTDIDLVVNTKKDFDTELYELA 300 TPTP DRNLASRLGDFAVR LI G S KACGII EL LTDID VVN+KK+F+ ELYELA Sbjct: 258 TPTPADRNLASRLGDFAVRMLIAGESAKACGIISNELVLTDIDKVVNSKKEFNMELYELA 317 Query: 301 SRLSQ 305 +RLSQ Sbjct: 318 ARLSQ 322 >B1DL07.1 B1DL07_9BACL 6-phosphofructokinase EC=2.7.1.11 Length = 322 Score = 473 bits (1218), Expect = e-132, Method: Compositional matrix adjust. Identities = 231/305 (75%), Positives = 267/305 (87%), Gaps = 1/305 (0%) Query: 1 MNAAVRAVVRSAIYFGIEVFGIQRGYQGLLNRDIFPMDLRSVGDIIQRGGTILQSARCLE 60 MNAAVRAVVRSA+Y G++V+G+QRGYQGL+N D+F MDLRSVGDIIQRGGTILQ+ARC E Sbjct: 19 MNAAVRAVVRSALYNGLDVYGVQRGYQGLINDDLFKMDLRSVGDIIQRGGTILQTARCKE 78 Query: 61 FLTPEGQQKGADILNEMGIDGLVVCGGDGSYKGANKLSKLGINTMALPGTIDNDISFTDY 120 F T EGQQ+GA++L E GIDGLVV GGDGSY+GANKLSKLGI TM LPGTIDNDI FTD+ Sbjct: 79 FTTLEGQQRGAEVLRERGIDGLVVIGGDGSYQGANKLSKLGIKTMGLPGTIDNDIPFTDF 138 Query: 121 TIGFDTAVGVVVDAINKLRDTMSSHERSSIVEVMGRHCGDIALHAGLASGAETILVPEMP 180 TIGFDTAV +VVDAINK+RDTMSSHERSS+VEVMGRHCGDIAL+AGLASGAETI+VPE+P Sbjct: 139 TIGFDTAVSIVVDAINKIRDTMSSHERSSVVEVMGRHCGDIALYAGLASGAETIIVPEVP 198 Query: 181 YDLNEVADRMRDNFARGKRHSIVIVAEGVGKGEDVAQALKDRHSSLDARVTVLGHIQRGG 240 D+ EV++RM+ NFA+GKRHSIV+VAEG G+GEDVA+ + + L+ RVTVLGHIQRGG Sbjct: 199 IDMAEVSERMKSNFAKGKRHSIVVVAEGAGRGEDVAREIT-KGCGLEPRVTVLGHIQRGG 257 Query: 241 TPTPGDRNLASRLGDFAVRSLIEGHSDKACGIIKGELTLTDIDLVVNTKKDFDTELYELA 300 TPT DR LASRLGDFAV L+EG S KACGI++GEL TDID VV+TKK F+ E+Y LA Sbjct: 258 TPTAMDRILASRLGDFAVHQLMEGESGKACGIVRGELVTTDIDKVVHTKKPFNMEMYNLA 317 Query: 301 SRLSQ 305 RLSQ Sbjct: 318 LRLSQ 322 >Q180P4.1 K6PF_CLOD6 6-phosphofructokinase EC=2.7.1.11 Length = 319 Score = 355 bits (912), Expect = 2e-96, Method: Compositional matrix adjust. Identities = 180/304 (59%), Positives = 227/304 (74%), Gaps = 1/304 (0%) Query: 1 MNAAVRAVVRSAIYFGIEVFGIQRGYQGLLNRDIFPMDLRSVGDIIQRGGTILQSARCLE 60 MNAA+RAVVRSAIY+G +V+GI RGY+GLL D+ M+L SVGDII RGGTIL+S+RC E Sbjct: 16 MNAAIRAVVRSAIYYGCKVYGINRGYKGLLEEDLTEMNLSSVGDIIHRGGTILKSSRCEE 75 Query: 61 FLTPEGQQKGADILNEMGIDGLVVCGGDGSYKGANKLSKLGINTMALPGTIDNDISFTDY 120 F T EG+ K IL + ID LVV GGDGS+ GA KLS LG + +PGTIDND+++TDY Sbjct: 76 FKTEEGRLKAVKILKKYKIDCLVVIGGDGSFAGAQKLSDLGFPAIGIPGTIDNDLAYTDY 135 Query: 121 TIGFDTAVGVVVDAINKLRDTMSSHERSSIVEVMGRHCGDIALHAGLASGAETILVPEMP 180 TIGFDTA+ ++DAI K+RDT SSHER +IVEVMGRHCGD+AL+AGLA GAETI+VPE+ Sbjct: 136 TIGFDTAMNTIIDAIGKIRDTSSSHERVNIVEVMGRHCGDLALYAGLAGGAETIIVPEVE 195 Query: 181 YDLNEVADRMRDNFARGKRHSIVIVAEGVGKGEDVAQALKDRHSSLDARVTVLGHIQRGG 240 ++EVA R++ RGKRHSI+++AEGVG D+ + LK + S D RVTVLGH+QRGG Sbjct: 196 ITVDEVALRLKTTQKRGKRHSIIVLAEGVGSASDLEKELK-KESGADLRVTVLGHVQRGG 254 Query: 241 TPTPGDRNLASRLGDFAVRSLIEGHSDKACGIIKGELTLTDIDLVVNTKKDFDTELYELA 300 +PT DR LASRLG AV L++G S + GI + ++ +I + KK FD E YE+A Sbjct: 255 SPTVSDRILASRLGVRAVELLLDGKSARVVGIKENKIIDLEISEALAQKKVFDKEAYEMA 314 Query: 301 SRLS 304 LS Sbjct: 315 KILS 318 >Q0SW30.1 Q0SW30_CLOPS 6-phosphofructokinase EC=2.7.1.11 Length = 319 Score = 347 bits (889), Expect = 1e-93, Method: Compositional matrix adjust. Identities = 170/305 (55%), Positives = 228/305 (74%), Gaps = 1/305 (0%) Query: 1 MNAAVRAVVRSAIYFGIEVFGIQRGYQGLLNRDIFPMDLRSVGDIIQRGGTILQSARCLE 60 MNAA+RAV R A++ G+EV G+QRGY GL+N ++F MD +SV +II RGGTIL++ARCLE Sbjct: 16 MNAALRAVTRMALHHGLEVMGVQRGYAGLINGELFKMDRKSVSEIINRGGTILRTARCLE 75 Query: 61 FLTPEGQQKGADILNEMGIDGLVVCGGDGSYKGANKLSKLGINTMALPGTIDNDISFTDY 120 F E ++K A IL G++ LVV GGDGS+ GA LSKLG+ T+ LPGTIDND+S+TDY Sbjct: 76 FKQEEVREKAAQILKAYGVEALVVIGGDGSFMGAKLLSKLGVKTVGLPGTIDNDLSYTDY 135 Query: 121 TIGFDTAVGVVVDAINKLRDTMSSHERSSIVEVMGRHCGDIALHAGLASGAETILVPEMP 180 TIGFDTA+ VVDAINKLRDT +SHER SIVEVMGR+CGD+AL+AG+A GAE I+VPEMP Sbjct: 136 TIGFDTALNTVVDAINKLRDTSTSHERVSIVEVMGRNCGDLALYAGIAGGAEAIIVPEMP 195 Query: 181 YDLNEVADRMRDNFARGKRHSIVIVAEGVGKGEDVAQALKDRHSSLDARVTVLGHIQRGG 240 +D +E+ + + GK HSI++VAEGVG ++A+ + + + ++ R T+LGHIQRGG Sbjct: 196 FDKDELIKTILEGRTNGKTHSIIVVAEGVGGSAELAKEI-ESVTGIETRATILGHIQRGG 254 Query: 241 TPTPGDRNLASRLGDFAVRSLIEGHSDKACGIIKGELTLTDIDLVVNTKKDFDTELYELA 300 +PT D LASR+G AV L+EG + K GI + + DID ++ ++ FD LYE+A Sbjct: 255 SPTADDIVLASRMGAKAVEVLLEGKTSKVIGIKENRIFDMDIDEALDIERSFDESLYEMA 314 Query: 301 SRLSQ 305 S +++ Sbjct: 315 SAINK 319 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.138 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,762,974,357 Number of extensions: 74980215 Number of successful extensions: 209000 Number of sequences better than 1.0e-05: 1943 Number of HSP's gapped: 205087 Number of HSP's successfully gapped: 2139 Length of query: 305 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 170 Effective length of database: 1,788,859,178 Effective search space: 304106060260 Effective search space used: 304106060260 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)