BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0345.0033.p (454 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KLC6.1 B3KLC6_9BACI MATE efflux family protein 554 e-156 A4J1I2.1 A4J1I2_DESRM MATE efflux family protein 468 e-130 C3B184.1 C3B184_BACMY Multidrug resistance protein norM 459 e-127 C3AJC8.1 C3AJC8_BACMY Multidrug resistance protein norM 459 e-127 C2ZLV3.1 C2ZLV3_BACCE Multidrug resistance protein norM 458 e-127 >B3KLC6.1 B3KLC6_9BACI MATE efflux family protein Length = 458 Score = 554 bits (1428), Expect = e-156, Method: Compositional matrix adjust. Identities = 278/454 (61%), Positives = 330/454 (72%) Query: 1 MIPTTSIKQKARQFLYILFPILITQIALSAITFFDTNMSGKFGTDDLAGVAIGTSLWIPI 60 M PTT+ + K +QF IL PIL+TQ+ALSAITFFDTNMSGKF + DLAGVAI TS+WIP+ Sbjct: 1 MQPTTTTRGKLKQFAIILIPILVTQLALSAITFFDTNMSGKFSSVDLAGVAIATSIWIPV 60 Query: 61 QTGLSGILMGITPLVSHLMGSKKEGDVAYQVTQGIWXXXXXXXXXXXXGTFALSPVLHFM 120 QTGLSGILMGITP+VS L+G ++E VAYQV Q +W G + +L M Sbjct: 61 QTGLSGILMGITPIVSQLVGGRQEQKVAYQVNQALWLSVLLAIVVLAIGYAVVPSILGAM 120 Query: 121 NLEPQVHDIAFRFLCAISVGIIPLFGYTVIRSCIDALGQTRVSMFITLIALPVNVGLNYL 180 NLEP+V IA FL AIS+GIIPLF YTV+RS IDALGQT++SM ITLI LPVNVGLN L Sbjct: 121 NLEPEVRRIASHFLAAISIGIIPLFAYTVLRSFIDALGQTKISMVITLITLPVNVGLNAL 180 Query: 181 LIFGKFGFPRLGGVGAGVASAITYWVIFGIALMFVYRTEPXXXXXXXXXXXXXXXXXXKE 240 LI+G +GFPRLGGVGAG+ASAITYW + IA++ ++R P K Sbjct: 181 LIYGYWGFPRLGGVGAGLASAITYWTVLAIAIVIIHRNHPFSPFGIFRQLRGVSLSAWKS 240 Query: 241 LLKIGVPIGFSIFFETAVFSAVTLLMSRFDTITIAAHQAAINFASTLYMIPLSICMSLTI 300 LLKIGVPIGFSIF ETAVF+AVTLLMS FDT TIAAHQAA+NFA+TLYM+PLSICMSLTI Sbjct: 241 LLKIGVPIGFSIFLETAVFAAVTLLMSSFDTATIAAHQAALNFATTLYMLPLSICMSLTI 300 Query: 301 LVGFENGAGRLKDARQYGIMGIGXXXXXXXXXXXXXXXXGSHVAGLYSDEPEVISLIRHF 360 LVGFE G+GR+KDA +Y +GIG G VAGLYS+EPEV++LI+HF Sbjct: 301 LVGFEAGSGRMKDAWKYAKLGIGTAVILSLITAVVLFMAGRQVAGLYSNEPEVVALIQHF 360 Query: 361 LIYAIFFQISDAIATPTQGVLRGYKDVNPAFIICFIAYWVIGLPTGYVLATYTSLAAYGY 420 L++AIFFQISDAIATPTQG LRGYKDVN AF++ F+++WVIGLP GYVLA YT A+GY Sbjct: 361 LLFAIFFQISDAIATPTQGALRGYKDVNVAFMLAFLSFWVIGLPVGYVLANYTDWQAFGY 420 Query: 421 WVXXXXXXXXXXXXXXSRLVKVQRQYAHNKPGTA 454 W+ RLVKVQR++A T+ Sbjct: 421 WIGLITGLGVGAALMLMRLVKVQRKFAGQAKATS 454 >A4J1I2.1 A4J1I2_DESRM MATE efflux family protein Length = 457 Score = 468 bits (1204), Expect = e-130, Method: Compositional matrix adjust. Identities = 240/447 (53%), Positives = 304/447 (68%) Query: 1 MIPTTSIKQKARQFLYILFPILITQIALSAITFFDTNMSGKFGTDDLAGVAIGTSLWIPI 60 M T S K+K QF+ IL+PILITQI+LSA+ DT MSG G DLAGVAIG+SLW+PI Sbjct: 1 MKKTFSTKEKILQFMTILWPILITQISLSAMVMLDTVMSGNAGARDLAGVAIGSSLWVPI 60 Query: 61 QTGLSGILMGITPLVSHLMGSKKEGDVAYQVTQGIWXXXXXXXXXXXXGTFALSPVLHFM 120 TG++GIL+ +TP+++ +G+ + V + V QG + G AL PVL+ M Sbjct: 61 STGITGILLAVTPIIAQSVGAGRRDHVPFTVIQGSYLAIAISIIVLICGAIALKPVLNTM 120 Query: 121 NLEPQVHDIAFRFLCAISVGIIPLFGYTVIRSCIDALGQTRVSMFITLIALPVNVGLNYL 180 +LE V DIA R+L +S GI PLF YT+ R IDALGQTRVSMFITL++LP+N+ LNY+ Sbjct: 121 HLESPVRDIARRYLVGLSFGITPLFIYTIFRCFIDALGQTRVSMFITLLSLPINILLNYV 180 Query: 181 LIFGKFGFPRLGGVGAGVASAITYWVIFGIALMFVYRTEPXXXXXXXXXXXXXXXXXXKE 240 LIFGK GFPRLGGVGAG A+AITYW +F IAL ++R EP + Sbjct: 181 LIFGKLGFPRLGGVGAGYATAITYWCVFFIALYVLHRVEPFSCYNFFKKWLGISFSAWRT 240 Query: 241 LLKIGVPIGFSIFFETAVFSAVTLLMSRFDTITIAAHQAAINFASTLYMIPLSICMSLTI 300 LK+G+PIGFSIFFET+VFS VTLLMS+++T+TIAAHQAA+NFAS LYM+PLSI M+LTI Sbjct: 241 QLKLGIPIGFSIFFETSVFSGVTLLMSQYNTVTIAAHQAALNFASFLYMVPLSISMALTI 300 Query: 301 LVGFENGAGRLKDARQYGIMGIGXXXXXXXXXXXXXXXXGSHVAGLYSDEPEVISLIRHF 360 +GFE GA R DA+QY ++G+G G VAGLY+ + V L + F Sbjct: 301 TIGFEVGAQRYSDAKQYSLLGLGIAVGMAVCCGIGLLLFGDQVAGLYTKDQTVFKLTQQF 360 Query: 361 LIYAIFFQISDAIATPTQGVLRGYKDVNPAFIICFIAYWVIGLPTGYVLATYTSLAAYGY 420 L+YA+FFQ+SDAIATP QG+LRGYKDVN +FI+ +YWVIGLP G++L+ YT LAA+GY Sbjct: 361 LVYALFFQLSDAIATPVQGILRGYKDVNVSFIVALASYWVIGLPLGHILSLYTPLAAFGY 420 Query: 421 WVXXXXXXXXXXXXXXSRLVKVQRQYA 447 WV R+V VQR A Sbjct: 421 WVGLIVGLAIGATSLLIRMVIVQRNAA 447 >C3B184.1 C3B184_BACMY Multidrug resistance protein norM Length = 452 Score = 459 bits (1182), Expect = e-127, Method: Compositional matrix adjust. Identities = 236/450 (52%), Positives = 300/450 (66%) Query: 1 MIPTTSIKQKARQFLYILFPILITQIALSAITFFDTNMSGKFGTDDLAGVAIGTSLWIPI 60 M T S QK +QF+ + FPI +TQ++L A++FFDT MSG DLAGVAIGTS+WIPI Sbjct: 1 MKETNSFSQKIKQFVLLFFPIFVTQMSLFAMSFFDTTMSGHASATDLAGVAIGTSIWIPI 60 Query: 61 QTGLSGILMGITPLVSHLMGSKKEGDVAYQVTQGIWXXXXXXXXXXXXGTFALSPVLHFM 120 TGL+GILM TP+++ L+GSK++ DV + V Q ++ G FA+SP+L M Sbjct: 61 STGLTGILMATTPIIAQLVGSKQKEDVPHIVIQAVYLAIGVSLLVILIGFFAVSPILKGM 120 Query: 121 NLEPQVHDIAFRFLCAISVGIIPLFGYTVIRSCIDALGQTRVSMFITLIALPVNVGLNYL 180 +LE QV IA +FL I++GIIPLF YTV+R IDALG+TR +M ITL++LP+NV LNY+ Sbjct: 121 HLEEQVEHIASQFLSIIAIGIIPLFVYTVLRGFIDALGKTRTTMIITLLSLPINVILNYV 180 Query: 181 LIFGKFGFPRLGGVGAGVASAITYWVIFGIALMFVYRTEPXXXXXXXXXXXXXXXXXXKE 240 IFG FGFP+LGGVGA +AS TYW I I ++ + EP KE Sbjct: 181 FIFGHFGFPKLGGVGAAIASTATYWCILFITIIIIRTKEPFTSFGIFRRLYRLSISSWKE 240 Query: 241 LLKIGVPIGFSIFFETAVFSAVTLLMSRFDTITIAAHQAAINFASTLYMIPLSICMSLTI 300 LLK+GVPIGF+IFFET++F+AVTL+MS F T TIAAHQAA+NFAS LYM PLS+ M++TI Sbjct: 241 LLKLGVPIGFAIFFETSIFAAVTLMMSNFSTTTIAAHQAAMNFASLLYMTPLSLAMAMTI 300 Query: 301 LVGFENGAGRLKDARQYGIMGIGXXXXXXXXXXXXXXXXGSHVAGLYSDEPEVISLIRHF 360 VGFE GA R ++A+QY +GIG +A +Y+ + V L + F Sbjct: 301 AVGFEVGANRCENAKQYSFIGIGLALAFALLYSILLFFFDDQIASIYTTDIAVHDLAKEF 360 Query: 361 LIYAIFFQISDAIATPTQGVLRGYKDVNPAFIICFIAYWVIGLPTGYVLATYTSLAAYGY 420 LI+AI FQISDAIATP QG LRGYKDVN I+ IAYW+IGLP GY+LATYTSLAA GY Sbjct: 361 LIFAILFQISDAIATPVQGALRGYKDVNVTLIMTLIAYWIIGLPLGYILATYTSLAAKGY 420 Query: 421 WVXXXXXXXXXXXXXXSRLVKVQRQYAHNK 450 W+ RL++VQR+Y K Sbjct: 421 WIGLIIGLAFGATFLLVRLLQVQRKYTSQK 450 >C3AJC8.1 C3AJC8_BACMY Multidrug resistance protein norM Length = 452 Score = 459 bits (1182), Expect = e-127, Method: Compositional matrix adjust. Identities = 236/450 (52%), Positives = 300/450 (66%) Query: 1 MIPTTSIKQKARQFLYILFPILITQIALSAITFFDTNMSGKFGTDDLAGVAIGTSLWIPI 60 M T S QK +QF+ + FPI +TQ++L A++FFDT MSG DLAGVAIGTS+WIPI Sbjct: 1 MKETNSFSQKIKQFVLLFFPIFVTQMSLFAMSFFDTTMSGHASATDLAGVAIGTSIWIPI 60 Query: 61 QTGLSGILMGITPLVSHLMGSKKEGDVAYQVTQGIWXXXXXXXXXXXXGTFALSPVLHFM 120 TGL+GILM TP+++ L+GSK++ DV + V Q ++ G FA+SP+L M Sbjct: 61 STGLTGILMATTPIIAQLVGSKQKEDVPHIVIQAVYLAIGVSLLVILIGFFAVSPILKGM 120 Query: 121 NLEPQVHDIAFRFLCAISVGIIPLFGYTVIRSCIDALGQTRVSMFITLIALPVNVGLNYL 180 +LE QV IA +FL I++GIIPLF YTV+R IDALG+TR +M ITL++LP+NV LNY+ Sbjct: 121 HLEEQVEHIASQFLSIIAIGIIPLFVYTVLRGFIDALGKTRTTMIITLLSLPINVILNYV 180 Query: 181 LIFGKFGFPRLGGVGAGVASAITYWVIFGIALMFVYRTEPXXXXXXXXXXXXXXXXXXKE 240 IFG FGFP+LGGVGA +AS TYW I I ++ + EP KE Sbjct: 181 FIFGHFGFPKLGGVGAAIASTATYWCILFITIIIIRTKEPFTSFGIFRRLYRLSISSWKE 240 Query: 241 LLKIGVPIGFSIFFETAVFSAVTLLMSRFDTITIAAHQAAINFASTLYMIPLSICMSLTI 300 LLK+GVPIGF+IFFET++F+AVTL+MS F T TIAAHQAA+NFAS LYM PLS+ M++TI Sbjct: 241 LLKLGVPIGFAIFFETSIFAAVTLMMSNFSTTTIAAHQAAMNFASLLYMTPLSLAMAMTI 300 Query: 301 LVGFENGAGRLKDARQYGIMGIGXXXXXXXXXXXXXXXXGSHVAGLYSDEPEVISLIRHF 360 VGFE GA R ++A+QY +GIG +A +Y+ + V L + F Sbjct: 301 AVGFEVGANRCENAKQYSFIGIGLALAFALLYSILLFFFDDQIASIYTTDIAVHDLAKEF 360 Query: 361 LIYAIFFQISDAIATPTQGVLRGYKDVNPAFIICFIAYWVIGLPTGYVLATYTSLAAYGY 420 LI+AI FQISDAIATP QG LRGYKDVN I+ IAYW+IGLP GY+LATYTSLAA GY Sbjct: 361 LIFAILFQISDAIATPVQGALRGYKDVNVTLIMTLIAYWIIGLPLGYILATYTSLAAKGY 420 Query: 421 WVXXXXXXXXXXXXXXSRLVKVQRQYAHNK 450 W+ RL++VQR+Y K Sbjct: 421 WIGLIIGLAFGATFLLVRLLQVQRKYTSQK 450 >C2ZLV3.1 C2ZLV3_BACCE Multidrug resistance protein norM Length = 457 Score = 458 bits (1178), Expect = e-127, Method: Compositional matrix adjust. Identities = 239/446 (53%), Positives = 297/446 (66%) Query: 1 MIPTTSIKQKARQFLYILFPILITQIALSAITFFDTNMSGKFGTDDLAGVAIGTSLWIPI 60 M TTS QK +QFL + FPI +TQ++L A++FFDT MSG DLAGVAIGTS+W+P+ Sbjct: 6 MKETTSFSQKLKQFLLLFFPIFVTQMSLFAMSFFDTTMSGHASPIDLAGVAIGTSIWLPV 65 Query: 61 QTGLSGILMGITPLVSHLMGSKKEGDVAYQVTQGIWXXXXXXXXXXXXGTFALSPVLHFM 120 TGL+GILM TP+V+ L+GSKK+ DV + V Q ++ G FA+SP+L+ M Sbjct: 66 STGLTGILMATTPIVAQLVGSKKKEDVPHIVIQAVYLAICASFVVILIGFFAVSPILNGM 125 Query: 121 NLEPQVHDIAFRFLCAISVGIIPLFGYTVIRSCIDALGQTRVSMFITLIALPVNVGLNYL 180 LE V IA +FL I++GIIPLF YTV+R IDALG+TR +M ITL++LP+NV LNYL Sbjct: 126 QLEEPVERIAAQFLSIIAIGIIPLFTYTVLRGFIDALGKTRTTMIITLLSLPINVVLNYL 185 Query: 181 LIFGKFGFPRLGGVGAGVASAITYWVIFGIALMFVYRTEPXXXXXXXXXXXXXXXXXXKE 240 LIFG FGFP+LGGVGA +ASA TYW I I ++ + EP KE Sbjct: 186 LIFGHFGFPKLGGVGAAIASAATYWCILIITVIIIRTKEPFASFNIFKQLYRPSFTSWKE 245 Query: 241 LLKIGVPIGFSIFFETAVFSAVTLLMSRFDTITIAAHQAAINFASTLYMIPLSICMSLTI 300 LK+GVPIGF+IFFET++F+AVTL+MS F T TIAAHQAA+NFAS LYM PLS+ M++TI Sbjct: 246 FLKLGVPIGFAIFFETSIFAAVTLMMSNFSTTTIAAHQAAMNFASLLYMTPLSLAMAMTI 305 Query: 301 LVGFENGAGRLKDARQYGIMGIGXXXXXXXXXXXXXXXXGSHVAGLYSDEPEVISLIRHF 360 VGFE GA R +A+QYG +GIG +A +Y+ V L + F Sbjct: 306 AVGFEVGAKRYNNAKQYGFIGIGLALAFALLYSILLYFFDDEIASIYTTNANVHHLAKEF 365 Query: 361 LIYAIFFQISDAIATPTQGVLRGYKDVNPAFIICFIAYWVIGLPTGYVLATYTSLAAYGY 420 LI+AI FQISDAIATP QG LRGYKDVN A I+ IAYWVIGLP GY+LATYT AA GY Sbjct: 366 LIFAILFQISDAIATPVQGALRGYKDVNVALIMTLIAYWVIGLPLGYILATYTDWAAKGY 425 Query: 421 WVXXXXXXXXXXXXXXSRLVKVQRQY 446 W+ RL +VQR+Y Sbjct: 426 WIGLIIGLAFGAIFLLIRLFQVQRKY 451 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.328 0.143 0.436 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,321,928,423 Number of extensions: 88836941 Number of successful extensions: 253912 Number of sequences better than 1.0e-05: 2469 Number of HSP's gapped: 249526 Number of HSP's successfully gapped: 2484 Length of query: 454 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 315 Effective length of database: 1,751,175,118 Effective search space: 551620162170 Effective search space used: 551620162170 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 133 (55.8 bits)