BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0345.0025.p (208 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KLD5.1 B3KLD5_9BACI Rhomboid family protein 231 2e-59 B1DL35.1 B1DL35_9BACL Rhomboid family protein 176 9e-43 P96617.1 P96617_BACSU YdcA protein 119 1e-25 A7Z1L8.1 A7Z1L8_BACA2 YdcA 118 3e-25 A6CSA4.1 A6CSA4_9BACI YdcA 116 1e-24 >B3KLD5.1 B3KLD5_9BACI Rhomboid family protein Length = 204 Score = 231 bits (590), Expect = 2e-59, Method: Compositional matrix adjust. Identities = 115/205 (56%), Positives = 151/205 (73%), Gaps = 3/205 (1%) Query: 1 MIFIRYEKWKGYIRYYPVTCILILLNVLMFIVLTANGGSTNLETLLKYGATLNAGPEMDE 60 MIF+RYE WK Y+RYYPVT +L+++N++MF++ + +GGS N TLLKYGA + +D+ Sbjct: 1 MIFVRYENWKSYLRYYPVTSLLLVINLVMFVITSIDGGSRNPMTLLKYGALSDLPQFVDQ 60 Query: 61 MWRYVTATFLHNGFAHLFFNCFALLVFAPPLERLMGWWRYALLYMAGGFIANYLXXXXXX 120 WRY TA FLHNGF HL FN FALLVF PPLER+MG W++A+LY+ G + N + Sbjct: 61 AWRYFTAMFLHNGFDHLLFNSFALLVFVPPLERIMGSWKFAILYLLSGVLGNVIGLAYYE 120 Query: 121 XXXXXXATVSVGASGAIYAVYGAFLYIALLQRGMMDENSRKTLYGLLVMGIIMSFATPYV 180 T VGASGAIY YGA+LYIAL Q+ ++DE+SRKTL+ LL++GI+ SF TP V Sbjct: 121 RMDSY--TFLVGASGAIYGAYGAYLYIALFQQHVIDESSRKTLFTLLILGILFSF-TPGV 177 Query: 181 NWMAHLSGLAAGFFLYGLIIRIFKR 205 + +AH+ GL GFFLYGL+IR+FKR Sbjct: 178 SLVAHVGGLVGGFFLYGLMIRLFKR 202 >B1DL35.1 B1DL35_9BACL Rhomboid family protein Length = 215 Score = 176 bits (447), Expect = 9e-43, Method: Compositional matrix adjust. Identities = 91/213 (42%), Positives = 135/213 (63%), Gaps = 5/213 (2%) Query: 1 MIFIRYEKWKGYIRYYPVTCILILLNVLMFIVLTANGGSTNLETLLKYGATLNAGPE--- 57 MIF+RYE ++GY+R +PVT ++I LN++ FIV+ G + L++ G ++ P+ Sbjct: 1 MIFLRYESFRGYLRAFPVTSVIIALNLIYFIVIAVTGDINDNYHLVQSGGFVSYMPDNPY 60 Query: 58 -MDEMWRYVTATFLHNGFAHLFFNCFALLVFAPPLERLMGWWRYALLYMAGGFIANYLXX 116 + E WR +T+ F+H+ F H+FFN F++LVFAPPLERL+ +YA+ Y+ G IAN L Sbjct: 61 GLKEPWRIITSMFMHSSFQHIFFNMFSILVFAPPLERLLKPVKYAVFYLLCGIIANLLTG 120 Query: 117 XXXXXXXXXXAT-VSVGASGAIYAVYGAFLYIALLQRGMMDENSRKTLYGLLVMGIIMSF 175 +S GASGAIY V+GAFLYIA+ ++ +D+ SRKT+Y +L G++ S Sbjct: 121 VVHGLFGDDRTIYLSTGASGAIYGVFGAFLYIAIFRKAWLDQGSRKTVYTILGFGLVFSV 180 Query: 176 ATPYVNWMAHLSGLAAGFFLYGLIIRIFKRSRR 208 P V+ + H+ G AGF LYGL+ R + RR Sbjct: 181 IVPSVSLLGHVGGGVAGFLLYGLLDRAMTKRRR 213 >P96617.1 P96617_BACSU YdcA protein Length = 199 Score = 119 bits (299), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 72/209 (34%), Positives = 110/209 (52%), Gaps = 13/209 (6%) Query: 2 IFIRYEKWKGYIRYYPVTCILILLNVLMFIVLTANGGSTNL--ETLLKYGATLNAGPEMD 59 +FIR E ++ +IR YPV ++ L ++++ + S L +T+ Y N G Sbjct: 1 MFIRTENFQTFIRLYPVVTFILALQAVLWLFFSLPAHSVVLWRDTVTGY----NLGVANG 56 Query: 60 EMWRYVTATFLHNGFAHLFFNCFALLVFAPPLERLMGWWRYALLYMAGGFIANYLXXXXX 119 E WR +T LH GF HL FN ++ +FAP LER++G R+ L+Y G I N Sbjct: 57 EWWRLITPILLHAGFTHLLFNSMSIFLFAPALERMLGKARFLLVYAGSGIIGN-----IG 111 Query: 120 XXXXXXXATVSVGASGAIYAVYGAFLYIALLQRGMMDENSRKTLYGLLVMGIIMSFATPY 179 V VGASGAI+ ++G +L++ L + ++ + K + LL ++MSF Sbjct: 112 TYVTEPLDYVHVGASGAIFGLFGVYLFMVLFRNELIGQEHSKMIITLLAFAVLMSFINSN 171 Query: 180 VNWMAHLSGLAAGFFLYGLIIRIFKRSRR 208 +N MAHL GL GF L L ++ K+ RR Sbjct: 172 INMMAHLFGLCGGFLLSFLCVQ--KKERR 198 >A7Z1L8.1 A7Z1L8_BACA2 YdcA Length = 199 Score = 118 bits (296), Expect = 3e-25, Method: Compositional matrix adjust. Identities = 72/208 (34%), Positives = 113/208 (54%), Gaps = 15/208 (7%) Query: 2 IFIRYEKWKGYIRYYP-VTCILILLNVLMFIVLTANGGSTNLETLLKYGATL---NAGPE 57 +FIR E ++ +IR YP V+CIL L L S L +++ + + N G Sbjct: 1 MFIRTENFQTFIRRYPAVSCILALQAFLWLFF------SMPLHSVMMWSDAVTGYNYGVS 54 Query: 58 MDEMWRYVTATFLHNGFAHLFFNCFALLVFAPPLERLMGWWRYALLYMAGGFIANYLXXX 117 E WR VT FLH F HL FN +L +FAPPLE+++G R+ ++Y+A G I N Sbjct: 55 SGEWWRLVTPMFLHANFTHLLFNSMSLFLFAPPLEQMLGKVRFLIVYIASGIIGN----- 109 Query: 118 XXXXXXXXXATVSVGASGAIYAVYGAFLYIALLQRGMMDENSRKTLYGLLVMGIIMSFAT 177 V VGASGAI+ ++G ++++ + ++ ++ + K + LL++ ++ +F Sbjct: 110 IGTYLAEPLDYVHVGASGAIFGLFGVYVFLTVFRKELIGRDQSKMIMTLLIIAVLSTFIN 169 Query: 178 PYVNWMAHLSGLAAGFFLYGLIIRIFKR 205 +N MAHL GLA GF L L ++ KR Sbjct: 170 SNINIMAHLFGLAGGFLLSFLCVQQKKR 197 >A6CSA4.1 A6CSA4_9BACI YdcA Length = 212 Score = 116 bits (290), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 66/209 (31%), Positives = 116/209 (55%), Gaps = 11/209 (5%) Query: 2 IFIRYEKWKGYIRYYPVTCILILLNVLMFIVLTANGGSTNLETLLKYG--ATLNAGPEMD 59 +F+R E + +IR YP+ I++ +++L+F V L +L Y A +N Sbjct: 12 LFVRTESFSQFIRLYPIVSIIVFIHLLLFSVALV----PFLPEILVYEHLAGINLYIFNG 67 Query: 60 EMWRYVTATFLHNGFAHLFFNCFALLVFAPPLERLMGWWRYALLYMAGGFIANYLXXXXX 119 E WR +T F+H GFAHL FN F+L++FAPPLER++G +++ ++Y+ G AN Sbjct: 68 EWWRLITPIFVHLGFAHLLFNSFSLILFAPPLERMLGKFKFTIIYLTCGIAAN-----IA 122 Query: 120 XXXXXXXATVSVGASGAIYAVYGAFLYIALLQRGMMDENSRKTLYGLLVMGIIMSFATPY 179 VGASGAI+ ++G ++ + L + ++ +++ + ++V+G++M+F Sbjct: 123 TYFLKPLTYNHVGASGAIFGLFGIYVGMTLFHKHLISTQNKQVIIPIVVIGLVMTFFQAN 182 Query: 180 VNWMAHLSGLAAGFFLYGLIIRIFKRSRR 208 +N AHL GL +G + L++ R R Sbjct: 183 INITAHLVGLLSGLVISWLLLPYMVRKNR 211 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.332 0.145 0.465 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,169,726,937 Number of extensions: 42964296 Number of successful extensions: 136962 Number of sequences better than 1.0e-05: 922 Number of HSP's gapped: 136216 Number of HSP's successfully gapped: 922 Length of query: 208 Length of database: 3,060,696,203 Length adjustment: 130 Effective length of query: 78 Effective length of database: 1,835,964,253 Effective search space: 143205211734 Effective search space used: 143205211734 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 128 (53.9 bits)