BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0345.0021.p (464 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KLD9.1 B3KLD9_9BACI Ammonium transporter 579 e-163 C0Z5S5.1 C0Z5S5_BREBN Ammonium transporter 510 e-143 C3J1B8.1 C3J1B8_9BACI Ammonium transporter 486 e-135 B7Y9T7.1 B7Y9T7_9BACI Ammonium transporter 486 e-135 B4BKH6.1 B4BKH6_9BACI Ammonium transporter 486 e-135 >B3KLD9.1 B3KLD9_9BACI Ammonium transporter Length = 479 Score = 579 bits (1492), Expect = e-163, Method: Compositional matrix adjust. Identities = 307/463 (66%), Positives = 357/463 (77%), Gaps = 3/463 (0%) Query: 1 MRKKMIMMFLAMITLMVYPVSAFAADG-PTAPDLQIGLDTAFTFLAFILVFFMQAGFAML 59 M+K+ + + +I L+ +P SAFAADG T +L GL++ F FLA +LVFFMQAGFA+L Sbjct: 1 MKKRWLGLMAVVIALLAFPASAFAADGGATNVELTAGLNSVFVFLAALLVFFMQAGFALL 60 Query: 60 EAGSVRMKNAGHVAGKTVLTLAIASLCFWAIXXXXXXXXXXXXXXXXXXLYGGDTQAASF 119 EAGSVRMKNAGHVAGKT LT I+ + FWA+ GD A SF Sbjct: 61 EAGSVRMKNAGHVAGKTFLTFGISIVAFWALGFGLAFGNGNSFFGFEGFFLSGDNAAGSF 120 Query: 120 DSLAFSDVTLNVKFLFQMAFAAVSLAIVSGGMAERAKLSVYIVFGILFSVVIYPVVAHWV 179 DSL+ DV + FLF ++FAAVSLAI GGMAERAKLSVYIVFG+LF++VIYP+VAHWV Sbjct: 121 DSLSAYDVPTTIMFLFHLSFAAVSLAIACGGMAERAKLSVYIVFGLLFTIVIYPIVAHWV 180 Query: 180 WGGGWLAELGMQDYAGSTVVHLTGATAAVVATILLKPRLGKFNKEGKPVIIPGHNQVFTV 239 WGGGWL LGMQD+AGSTVVHLTGATAA+VATILLKPRLGK+NK+GKP IPGHNQV +V Sbjct: 181 WGGGWLGALGMQDFAGSTVVHLTGATAALVATILLKPRLGKYNKDGKPNSIPGHNQVLSV 240 Query: 240 LGVIILWFGWFGFNPGSALSPM-GGFFGHVALTTNXXXXXXXXXXXXXSWLYFGKSDIPA 298 LGV I+W GWFGFNPGS +S + GFFG VAL TN SW GK+DIP+ Sbjct: 241 LGVFIIWIGWFGFNPGSTISALTDGFFGFVALNTNIAAAAGALAALLISWAVLGKADIPS 300 Query: 299 MLNGVLAALVAITGACAFVEPWAAILIGFVAGAFTFMTSQWLERAGLDDPIYAFSVHGIA 358 MLNG+LAA VAITGACAFVEPWAA++IG VAG TF T+QW +R G+DDP+YAFSVHGIA Sbjct: 301 MLNGILAAFVAITGACAFVEPWAAVVIGAVAGTVTFFTAQWFDRKGIDDPVYAFSVHGIA 360 Query: 359 GMWGAVSTGLFATPDLITKGALVGKAGLFYGGGFHQLGVQILGVAGTFVFVAVMSFIILY 418 GMWGAVSTGLFA P L+ VGK GLFYGGGF QLGVQ+LG+ GTF FV V+SFIILY Sbjct: 361 GMWGAVSTGLFAAPRLVEITG-VGKEGLFYGGGFGQLGVQLLGLIGTFAFVLVISFIILY 419 Query: 419 VMKLVIGLRVTEEEELMGLDISEHGTYGYPEQMKLIAESESKT 461 +MK+ +G+RVTEEEELMGLDISEHGTYGYPEQMKL+ ESE K+ Sbjct: 420 IMKVTMGIRVTEEEELMGLDISEHGTYGYPEQMKLLVESEGKS 462 >C0Z5S5.1 C0Z5S5_BREBN Ammonium transporter Length = 444 Score = 510 bits (1314), Expect = e-143, Method: Compositional matrix adjust. Identities = 254/425 (59%), Positives = 318/425 (74%), Gaps = 2/425 (0%) Query: 28 PTAPDLQIGLDTAFTFLAFILVFFMQAGFAMLEAGSVRMKNAGHVAGKTVLTLAIASLCF 87 PT L +D + ++ ILV MQ GFA+LEAGS RMKNAGHVAGKTVLT I S+ F Sbjct: 3 PTVASLSSAIDATWVMVSAILVILMQVGFALLEAGSTRMKNAGHVAGKTVLTFGICSIAF 62 Query: 88 WAIXXXXXXXXXXXXXXXXXXLYGGDTQAASFDSLAFSDVTLNVKFLFQMAFAAVSLAIV 147 WA + G Q +F + + + V +++ FLFQ+AFAAVSLAI Sbjct: 63 WAFGFGLTFGDGNSFIGLSGFFFDG-MQKDAFSAFSAATVPISILFLFQLAFAAVSLAIA 121 Query: 148 SGGMAERAKLSVYIVFGILFSVVIYPVVAHWVWGGGWLAELGMQDYAGSTVVHLTGATAA 207 GG AERAKLS+Y +F +LF+V+IYPVV HWVWGGGWLA+LGMQD+AGSTVVHL GA A Sbjct: 122 WGGFAERAKLSIYFLFSVLFTVLIYPVVGHWVWGGGWLAQLGMQDFAGSTVVHLQGAIVA 181 Query: 208 VVATILLKPRLGKFNKEGKPVIIPGHNQVFTVLGVIILWFGWFGFNPGSALSPMGGFFGH 267 +VATILLKPR+GK+N++G +IPGHNQV++VLGV+ LW GWFGFN GS L GFFG+ Sbjct: 182 LVATILLKPRIGKYNRDGSSNLIPGHNQVYSVLGVLFLWIGWFGFNAGSTLGSTSGFFGY 241 Query: 268 VALTTNXXXXXXXXXXXXXSWLYFGKSDIPAMLNGVLAALVAITGACAFVEPWAAILIGF 327 +A+TTN SW+ GK+DIP+MLNGVLAALVAIT +CAFV+PWAA++IG Sbjct: 242 IAVTTNLATAAGAVAALAISWIVMGKADIPSMLNGVLAALVAITASCAFVDPWAAVVIGS 301 Query: 328 VAGAFTFMTSQWLERAGLDDPIYAFSVHGIAGMWGAVSTGLFATPDLITKGALVGKAGLF 387 V+G TF TS W +R G+DDP++AFSVHG+AG+WG +STGLFATP+L+ K VG GLF Sbjct: 302 VSGILTFYTSIWFDRMGIDDPVFAFSVHGVAGIWGTLSTGLFATPELVEKVG-VGSPGLF 360 Query: 388 YGGGFHQLGVQILGVAGTFVFVAVMSFIILYVMKLVIGLRVTEEEELMGLDISEHGTYGY 447 YGGG HQLGVQ LG+AG+ ++V V++FIILYV+K+ IGLRVTEEEE++GLD+SEHG YGY Sbjct: 361 YGGGLHQLGVQALGLAGSALYVFVVAFIILYVLKVTIGLRVTEEEEVVGLDLSEHGGYGY 420 Query: 448 PEQMK 452 PE ++ Sbjct: 421 PELLQ 425 >C3J1B8.1 C3J1B8_9BACI Ammonium transporter Length = 433 Score = 486 bits (1252), Expect = e-135, Method: Compositional matrix adjust. Identities = 257/432 (59%), Positives = 316/432 (73%), Gaps = 5/432 (1%) Query: 33 LQIGLDTAFTFLAFILVFFMQAGFAMLEAGSVRMKNAGHVAGKTVLTLAIASLCFWAIXX 92 L +GLD + L+ +LV MQAGFA+LEAGS RMKN+GHVAGK +L+ AIASL FWA Sbjct: 6 LTLGLDALWVMLSSVLVIGMQAGFALLEAGSTRMKNSGHVAGKQILSFAIASLAFWAFGF 65 Query: 93 XXXXXXXXXXXXXXXXLYGGDTQAASFDSLAFSDVTLNVKFLFQMAFAAVSLAIVSGGMA 152 + +FDSL++++V L +KFLFQ+ FA VSLAI GG A Sbjct: 66 AITFGAGNSFIGTEGWFLKEGKE--TFDSLSWANVPLELKFLFQLGFAGVSLAIAWGGFA 123 Query: 153 ERAKLSVYIVFGILFSVVIYPVVAHWVWGGGWLAELGMQDYAGSTVVHLTGATAAVVATI 212 ERAKLS+Y +FG +F++ IYPV+ HWVWGGGWL ++GMQD+AGSTVVHL GA AA+VATI Sbjct: 124 ERAKLSIYFLFGTIFTIAIYPVIGHWVWGGGWLGKMGMQDFAGSTVVHLQGAIAALVATI 183 Query: 213 LLKPRLGKFNKEGKPVIIPGHNQVFTVLGVIILWFGWFGFNPGSALSPMGGFFGHVALTT 272 LL PR+GKFNK+ P +IPGHNQV+TV+G +ILW GWFGFN GS ++ GFFG+VALTT Sbjct: 184 LLGPRIGKFNKDKTPNVIPGHNQVYTVIGGLILWIGWFGFNAGSTMAVGDGFFGYVALTT 243 Query: 273 NXXXXXXXXXXXXXSWLYFGKSDIPAMLNGVLAALVAITGACAFVEPWAAILIGFVAGAF 332 N + + GK+DIPAM+NGVLAALVAIT ACAFVEPWAA++IG VAG+F Sbjct: 244 NLAAAAGAIAAIVTAKILVGKADIPAMVNGVLAALVAITAACAFVEPWAAVVIGAVAGSF 303 Query: 333 TFMTSQWLERAGLDDPIYAFSVHGIAGMWGAVSTGLFATPDLITKGALVGKAGLFYGGGF 392 TF TS + ER G+DDPIYAFSVHG+AG+ G +STG FA+P L+ +GKAGLFYGGGF Sbjct: 304 TFWTSTYFERKGIDDPIYAFSVHGVAGIIGTISTGFFASPRLVEITG-IGKAGLFYGGGF 362 Query: 393 HQLGVQILGVAGTFVFVAVMSFIILYVMKLVIGLRVTEEEELMGLDISEHGTYGYPEQMK 452 QL VQ +GV G V+VA +SF+ILY MK IGLRVT E+E+ GLDISEHG+YGYPEQ+ Sbjct: 363 DQLIVQTVGVLGAAVYVAAVSFVILYAMKKTIGLRVTAEQEISGLDISEHGSYGYPEQLD 422 Query: 453 LIAESESKTLKH 464 + KTL Sbjct: 423 --PAYQPKTLAQ 432 >B7Y9T7.1 B7Y9T7_9BACI Ammonium transporter Length = 433 Score = 486 bits (1252), Expect = e-135, Method: Compositional matrix adjust. Identities = 257/432 (59%), Positives = 316/432 (73%), Gaps = 5/432 (1%) Query: 33 LQIGLDTAFTFLAFILVFFMQAGFAMLEAGSVRMKNAGHVAGKTVLTLAIASLCFWAIXX 92 L +GLD + L+ +LV MQAGFA+LEAGS RMKN+GHVAGK +L+ AIASL FWA Sbjct: 6 LTLGLDALWVMLSSVLVIGMQAGFALLEAGSTRMKNSGHVAGKQILSFAIASLAFWAFGF 65 Query: 93 XXXXXXXXXXXXXXXXLYGGDTQAASFDSLAFSDVTLNVKFLFQMAFAAVSLAIVSGGMA 152 + +FDSL++++V L +KFLFQ+ FA VSLAI GG A Sbjct: 66 AITFGAGNSFIGTEGWFLKEGKE--TFDSLSWANVPLELKFLFQLGFAGVSLAIAWGGFA 123 Query: 153 ERAKLSVYIVFGILFSVVIYPVVAHWVWGGGWLAELGMQDYAGSTVVHLTGATAAVVATI 212 ERAKLS+Y +FG +F++ IYPV+ HWVWGGGWL ++GMQD+AGSTVVHL GA AA+VATI Sbjct: 124 ERAKLSIYFLFGTIFTIAIYPVIGHWVWGGGWLGKMGMQDFAGSTVVHLQGAIAALVATI 183 Query: 213 LLKPRLGKFNKEGKPVIIPGHNQVFTVLGVIILWFGWFGFNPGSALSPMGGFFGHVALTT 272 LL PR+GKFNK+ P +IPGHNQV+TV+G +ILW GWFGFN GS ++ GFFG+VALTT Sbjct: 184 LLGPRIGKFNKDKTPNVIPGHNQVYTVIGGLILWIGWFGFNAGSTMAVGDGFFGYVALTT 243 Query: 273 NXXXXXXXXXXXXXSWLYFGKSDIPAMLNGVLAALVAITGACAFVEPWAAILIGFVAGAF 332 N + + GK+DIPAM+NGVLAALVAIT ACAFVEPWAA++IG VAG+F Sbjct: 244 NLAAAAGAIAAIVTAKILVGKADIPAMVNGVLAALVAITAACAFVEPWAAVVIGAVAGSF 303 Query: 333 TFMTSQWLERAGLDDPIYAFSVHGIAGMWGAVSTGLFATPDLITKGALVGKAGLFYGGGF 392 TF TS + ER G+DDPIYAFSVHG+AG+ G +STG FA+P L+ +GKAGLFYGGGF Sbjct: 304 TFWTSTYFERKGIDDPIYAFSVHGVAGIIGTISTGFFASPRLVEITG-IGKAGLFYGGGF 362 Query: 393 HQLGVQILGVAGTFVFVAVMSFIILYVMKLVIGLRVTEEEELMGLDISEHGTYGYPEQMK 452 QL VQ +GV G V+VA +SF+ILY MK IGLRVT E+E+ GLDISEHG+YGYPEQ+ Sbjct: 363 DQLIVQTVGVLGAAVYVAAVSFVILYAMKKTIGLRVTAEQEISGLDISEHGSYGYPEQLD 422 Query: 453 LIAESESKTLKH 464 + KTL Sbjct: 423 --PAYQPKTLAQ 432 >B4BKH6.1 B4BKH6_9BACI Ammonium transporter Length = 433 Score = 486 bits (1252), Expect = e-135, Method: Compositional matrix adjust. Identities = 259/431 (60%), Positives = 315/431 (73%), Gaps = 3/431 (0%) Query: 33 LQIGLDTAFTFLAFILVFFMQAGFAMLEAGSVRMKNAGHVAGKTVLTLAIASLCFWAIXX 92 L GLD + LA +LV MQAGFA+LEAGS RMKN+GHVAGK +L+ A+ASL FWA Sbjct: 6 LMFGLDALWVMLAAVLVIGMQAGFALLEAGSTRMKNSGHVAGKQILSFALASLAFWAFGF 65 Query: 93 XXXXXXXXXXXXXXXXLYGGDTQAASFDSLAFSDVTLNVKFLFQMAFAAVSLAIVSGGMA 152 +FDSL++++V L +KFLFQ+ FA VSLAI GG A Sbjct: 66 AITFGAGNGFIGTEGWFL--QEGEGTFDSLSWANVPLALKFLFQLGFAGVSLAIAWGGFA 123 Query: 153 ERAKLSVYIVFGILFSVVIYPVVAHWVWGGGWLAELGMQDYAGSTVVHLTGATAAVVATI 212 ERAKLSVY VFG +F++VIYPV+ HWVWGGGWL ++GMQD+AGSTVVHL GA AA+VAT+ Sbjct: 124 ERAKLSVYFVFGTIFTIVIYPVIGHWVWGGGWLGQMGMQDFAGSTVVHLQGAIAALVATV 183 Query: 213 LLKPRLGKFNKEGKPVIIPGHNQVFTVLGVIILWFGWFGFNPGSALSPMGGFFGHVALTT 272 LL PR+GKFNK+ P +IPGHNQV+TV+G +ILW GWFGFN GS ++ GFFG+VALTT Sbjct: 184 LLGPRIGKFNKDKTPNVIPGHNQVYTVIGGLILWIGWFGFNAGSTMAVGDGFFGYVALTT 243 Query: 273 NXXXXXXXXXXXXXSWLYFGKSDIPAMLNGVLAALVAITGACAFVEPWAAILIGFVAGAF 332 N + + GK+DIPAM+NGVLAALVAIT ACAFVEPWAA++IG VAG+F Sbjct: 244 NLAAAAGAIAAILTAKVLVGKADIPAMVNGVLAALVAITAACAFVEPWAAVIIGAVAGSF 303 Query: 333 TFMTSQWLERAGLDDPIYAFSVHGIAGMWGAVSTGLFATPDLITKGALVGKAGLFYGGGF 392 TF TS + ER G+DDPIYAFSVHGIAG+ G +STG FA+P L+ VGKAGL YGGGF Sbjct: 304 TFWTSVYFERKGIDDPIYAFSVHGIAGIIGTISTGFFASPRLVEITG-VGKAGLVYGGGF 362 Query: 393 HQLGVQILGVAGTFVFVAVMSFIILYVMKLVIGLRVTEEEELMGLDISEHGTYGYPEQMK 452 QL VQ +GV G ++VAV+SF ILYVMK IGLRVT E+E+ GLDISEHG YGYPEQ+ Sbjct: 363 DQLIVQTVGVVGAAIYVAVVSFAILYVMKKTIGLRVTAEQEISGLDISEHGAYGYPEQLD 422 Query: 453 LIAESESKTLK 463 + ++ T + Sbjct: 423 PAYQPKTATQR 433 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.327 0.141 0.435 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,535,107,105 Number of extensions: 103288481 Number of successful extensions: 388007 Number of sequences better than 1.0e-05: 2260 Number of HSP's gapped: 378193 Number of HSP's successfully gapped: 2297 Length of query: 464 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 325 Effective length of database: 1,751,175,118 Effective search space: 569131913350 Effective search space used: 569131913350 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 133 (55.8 bits)