BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0345.0016.p (539 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C0ZI85.1 C0ZI85_BREBN 2-isopropylmalate synthase/homocitrate syn... 625 e-177 Q2RGA1.1 Q2RGA1_MOOTA 2-isopropylmalate synthase EC=2.3.3.13 616 e-174 B0TCR5.1 B0TCR5_HELMI 2-isopropylmalate synthase/homocitrate syn... 601 e-170 B3KHL2.1 B3KHL2_9BACI LeuA allosteric (Dimerisation) domain 592 e-167 A5D4W4.1 A5D4W4_PELTS Isopropylmalate/homocitrate/citramalate sy... 587 e-165 >C0ZI85.1 C0ZI85_BREBN 2-isopropylmalate synthase/homocitrate synthase related family protein Length = 540 Score = 625 bits (1611), Expect = e-177, Method: Compositional matrix adjust. Identities = 304/541 (56%), Positives = 391/541 (72%), Gaps = 3/541 (0%) Query: 1 MSKSISIFDTTLRDGTQGEGISXXXXXXXXXXXXXXXXGVHYIEGGIPGSNNKDIEFFKR 60 M+ + ++DTTLRDGTQGEGIS G+ +IEGG PGSN KD+ FF+R Sbjct: 1 MNSKVYLYDTTLRDGTQGEGISLSVEDKIKIALRLDQFGIDFIEGGWPGSNPKDMAFFER 60 Query: 61 VKELHL-HAKITAFGSTRRKNSITEHDENLQRMIDAGVPAATLVGKSWDFHVHTALQTTL 119 ++E+ L HAK+TAFGST R N D+NLQ +I +G AA + GKSWD HV AL+TTL Sbjct: 61 IREISLQHAKVTAFGSTCRPNVAAADDDNLQALILSGASAAAIFGKSWDLHVTDALKTTL 120 Query: 120 EENLAMIGESIAYLKRKGLEVIFDAEHFFDGYKNNPEYXXXXXXXXXXXXXDWLVMCDTN 179 EEN+ M+ +S+A+LK GL +F AEHFFDGYK NP Y DW+V+CDTN Sbjct: 121 EENVRMVADSVAFLKENGLTTLFLAEHFFDGYKANPRYALRVLEAAEEAGADWIVLCDTN 180 Query: 180 GGTLPNEIQEIVSXXXXXXXXXXXXIHTHNDCELAVANTLSAISAGARQVQGTINGYGER 239 GGTLP+E+ +IV IH HND +AVAN L+AI AGA+QVQGTING GER Sbjct: 181 GGTLPHEVYDIVKTTVGHLRTPVG-IHPHNDSGVAVANALAAIQAGAQQVQGTINGIGER 239 Query: 240 CGNANLCSIIPTLQLKMGYHCIPGDSLPQLTNTAHYISEVANVNLPVNQPYVGNAAFAHK 299 CGN NL S++P LQLK+G+HC+ D L +LT + Y++E+AN+ +P NQP+VG +AFAHK Sbjct: 240 CGNVNLISVVPNLQLKLGHHCVSSDQLQELTQLSRYVAEIANMTMPNNQPFVGYSAFAHK 299 Query: 300 GGIHVSAILRDSRTYEHIAPELVGNKQRVLVSELAGQSNVLSKAQDMGLSLDPSSEQARK 359 GGIHVSA++RD +TYEHI PE +GNK+RVLVSELAGQSN+L+K +++ + L E+AR+ Sbjct: 300 GGIHVSAVMRDPKTYEHIEPENIGNKRRVLVSELAGQSNLLAKMEELEIDLSLDREKARE 359 Query: 360 VIDKIKNLEHQGYQFEGADASLELLLREATGEMNELFTFESFKMLVEKTAGSPVVSEAFV 419 +I IK E QGYQ+EGA+ASL L+L EA+G++ LF +SFK+++EK A + SEA V Sbjct: 360 IITHIKEREFQGYQYEGAEASLTLMLLEASGKLKNLFKLDSFKIMLEKAAIQSITSEATV 419 Query: 420 KLKVGGDNLYTAAEGNGPVNALDNALRKALLTYFPQLKDMHLSDYKVRVLDEKDQTAAKV 479 KL+V G++++TAA+GNGPVNALDNA+RKAL +Y+P + M L DYKVRVLDE TAAKV Sbjct: 420 KLRVNGESVHTAADGNGPVNALDNAMRKALESYYPCIAKMQLEDYKVRVLDENGATAAKV 479 Query: 480 RVLIESKDYSDTWNTVGVSSNVIEASWEALVDSMRYALLGQISLENGTEISSGPR-GLVN 538 RVLIES + W+TVGVS+NVIEASWEAL DS+RY L + E G+ SSG R G+VN Sbjct: 480 RVLIESSSNGEKWSTVGVSTNVIEASWEALADSIRYLLWKEGCEEVGSLASSGTRVGIVN 539 Query: 539 H 539 H Sbjct: 540 H 540 >Q2RGA1.1 Q2RGA1_MOOTA 2-isopropylmalate synthase EC=2.3.3.13 Length = 533 Score = 616 bits (1589), Expect = e-174, Method: Compositional matrix adjust. Identities = 305/521 (58%), Positives = 376/521 (72%), Gaps = 4/521 (0%) Query: 1 MSKSISIFDTTLRDGTQGEGISXXXXXXXXXXXXXXXXGVHYIEGGIPGSNNKDIEFFKR 60 M++ I I+DTTLRDG+QGEGIS GV YIEGG P +N KD+EFF R Sbjct: 1 MAEKILIYDTTLRDGSQGEGISLSVEDKLKIASRLDRLGVDYIEGGWPWANPKDMEFFLR 60 Query: 61 VKE-LHLHAKITAFGSTRRKNSITEHDENLQRMIDAGVPAATLVGKSWDFHVHTALQTTL 119 +E + A++ AFG TR+ D NL + AGV AT+ GKSWD HV AL TTL Sbjct: 61 AREVIWRQARLVAFGRTRKPGQAAAEDANLLAIKRAGVKVATIFGKSWDLHVTAALGTTL 120 Query: 120 EENLAMIGESIAYLKRKGLEVIFDAEHFFDGYKNNPEYXXXXXXXXXXXXXDWLVMCDTN 179 ENLAMIG+S+A+L +GLEVI+DAEHFFDG+K NP+Y W+V+CDTN Sbjct: 121 AENLAMIGDSVAFLVDQGLEVIYDAEHFFDGFKANPDYALETLKAAAKAGASWIVLCDTN 180 Query: 180 GGTLPNEIQEIVSXXXXXXXXXXXXIHTHNDCELAVANTLSAISAGARQVQGTINGYGER 239 GG LP EI+E V+ IH HND +LAVANTL+A++AG RQVQGTING+GER Sbjct: 181 GGCLPWEIEEAVARVRQEIQVPVG-IHAHNDGDLAVANTLAAVTAGCRQVQGTINGFGER 239 Query: 240 CGNANLCSIIPTLQLKMGYHCIPGDSLPQLTNTAHYISEVANVNLPVNQPYVGNAAFAHK 299 CGNA+LCS++P L+LKMGY C+P L LT + Y+SE+ANV NQP+VG +AFAHK Sbjct: 240 CGNADLCSVMPNLELKMGYQCLPPGQLAFLTEVSRYVSEIANVVPAGNQPFVGYSAFAHK 299 Query: 300 GGIHVSAILRDSRTYEHIAPELVGNKQRVLVSELAGQSNVLSKAQDMGLSLDPSSEQARK 359 GGIHVSA+L+ TYEHI P+ VGN++RVL+S+ AG SN+ KA++MGL L+P E+ R Sbjct: 300 GGIHVSAVLKAPDTYEHIRPQQVGNERRVLMSDQAGASNLRCKAEEMGLELNP--ERERG 357 Query: 360 VIDKIKNLEHQGYQFEGADASLELLLREATGEMNELFTFESFKMLVEKTAGSPVVSEAFV 419 +I+ IK LE QGYQFEGADASLEL LR+ TGE + F E K LVEK AG +SEA V Sbjct: 358 IIEGIKELERQGYQFEGADASLELFLRKTTGEYRQQFEVEYVKALVEKRAGQEAISEAIV 417 Query: 420 KLKVGGDNLYTAAEGNGPVNALDNALRKALLTYFPQLKDMHLSDYKVRVLDEKDQTAAKV 479 KL+VG ++TAAEGNGPVNA+DNALRKAL FP ++ M L+DYKVRVLDEKD T+A+V Sbjct: 418 KLRVGDQVVHTAAEGNGPVNAMDNALRKALEEVFPAIRHMRLTDYKVRVLDEKDATSARV 477 Query: 480 RVLIESKDYSDTWNTVGVSSNVIEASWEALVDSMRYALLGQ 520 RVLIES+D S++WNTVGVS+N+IEASWEAL+DSM YALL Q Sbjct: 478 RVLIESRDGSNSWNTVGVSTNIIEASWEALLDSMEYALLKQ 518 >B0TCR5.1 B0TCR5_HELMI 2-isopropylmalate synthase/homocitrate synthase family protein, putative Length = 531 Score = 601 bits (1549), Expect = e-170, Method: Compositional matrix adjust. Identities = 287/515 (55%), Positives = 373/515 (72%), Gaps = 2/515 (0%) Query: 5 ISIFDTTLRDGTQGEGISXXXXXXXXXXXXXXXXGVHYIEGGIPGSNNKDIEFFKRVKEL 64 + I+DTTLRDGTQGEGIS GV YIEGG PGSN KD+EFF+R +++ Sbjct: 4 VFIYDTTLRDGTQGEGISLSVEDKVKIAARLDQLGVAYIEGGWPGSNPKDMEFFQRAQQM 63 Query: 65 HL-HAKITAFGSTRRKNSITEHDENLQRMIDAGVPAATLVGKSWDFHVHTALQTTLEENL 123 HAKI AFGST R S D NL+ +I++G P T+ GKSWDFHV AL+TTLEENL Sbjct: 64 TWKHAKIAAFGSTCRPGSEACDDPNLRALIESGAPVVTIFGKSWDFHVTAALRTTLEENL 123 Query: 124 AMIGESIAYLKRKGLEVIFDAEHFFDGYKNNPEYXXXXXXXXXXXXXDWLVMCDTNGGTL 183 ++ +SI +LK +G EVIFDAEHF+DGYK NP Y DW+V+CDTNGGTL Sbjct: 124 RLVRDSITFLKNQGREVIFDAEHFYDGYKGNPAYAKEVVMTAEKAGADWIVLCDTNGGTL 183 Query: 184 PNEIQEIVSXXXXXXXXXXXXIHTHNDCELAVANTLSAISAGARQVQGTINGYGERCGNA 243 P+++ I + +H HND +LAVAN++ + AGARQVQGT+NGYGERCGN Sbjct: 184 PHDVLSI-TQEMVFTLRAPVGVHVHNDGDLAVANSIMGVMAGARQVQGTMNGYGERCGNV 242 Query: 244 NLCSIIPTLQLKMGYHCIPGDSLPQLTNTAHYISEVANVNLPVNQPYVGNAAFAHKGGIH 303 NLCS+IP LQLKM C+P L LT+ AH++ E+AN+ L + P+VG++AFAHKGGIH Sbjct: 243 NLCSVIPNLQLKMKMECLPEGKLQTLTDAAHFVGEIANMPLRNDMPFVGHSAFAHKGGIH 302 Query: 304 VSAILRDSRTYEHIAPELVGNKQRVLVSELAGQSNVLSKAQDMGLSLDPSSEQARKVIDK 363 VSA+++D TYEHI PE VGN +RVLVSEL+G SNV+ KA+++GL ++ + +++I++ Sbjct: 303 VSALMKDPGTYEHIQPEAVGNHRRVLVSELSGMSNVIYKAKELGLDVNRQNADTKQIIEQ 362 Query: 364 IKNLEHQGYQFEGADASLELLLREATGEMNELFTFESFKMLVEKTAGSPVVSEAFVKLKV 423 IKNLEHQG+QFEGA+AS E+LLR A GE F +S ++++EK + + SEA +KL+V Sbjct: 363 IKNLEHQGFQFEGAEASFEVLLRRAFGEDPVPFVLDSIRLIIEKRSDADFTSEAMIKLRV 422 Query: 424 GGDNLYTAAEGNGPVNALDNALRKALLTYFPQLKDMHLSDYKVRVLDEKDQTAAKVRVLI 483 G ++TAAEGNGPVNA+DNALRKALL+++P L + HL+DYKVRVLD KD T AKVRVLI Sbjct: 423 GDQVVHTAAEGNGPVNAVDNALRKALLSHYPFLAECHLTDYKVRVLDGKDATEAKVRVLI 482 Query: 484 ESKDYSDTWNTVGVSSNVIEASWEALVDSMRYALL 518 E++D D W TVGVS+N+IEASW+AL+DS Y + Sbjct: 483 ETRDSHDAWGTVGVSTNIIEASWQALMDSFLYGYM 517 >B3KHL2.1 B3KHL2_9BACI LeuA allosteric (Dimerisation) domain Length = 364 Score = 592 bits (1527), Expect = e-167, Method: Compositional matrix adjust. Identities = 286/365 (78%), Positives = 319/365 (87%), Gaps = 1/365 (0%) Query: 175 MCDTNGGTLPNEIQEIVSXXXXXXXXXXXXIHTHNDCELAVANTLSAISAGARQVQGTIN 234 MCDTNGG+LP+EI +IV+ IHTHNDCELAVANTLSA+ AG R VQGT+N Sbjct: 1 MCDTNGGSLPHEISDIVTSLTGPLPGAPLGIHTHNDCELAVANTLSAVQAGVRHVQGTMN 60 Query: 235 GYGERCGNANLCSIIPTLQLKMGYHCIPGDSLPQLTNTAHYISEVANVNLPVNQPYVGNA 294 GYGERCGNANLCSIIP LQLK+GY CI D+L LTNTA YISE+ANVN+PVNQPYVGNA Sbjct: 61 GYGERCGNANLCSIIPNLQLKLGYECIGDDNLRTLTNTARYISEIANVNMPVNQPYVGNA 120 Query: 295 AFAHKGGIHVSAILRDSRTYEHIAPELVGNKQRVLVSELAGQSNVLSKAQDMGLSLDPSS 354 AFAHKGGIHVSAILRDSRTYEHI PE VGNKQRVLVSELAGQSN++SKAQDMGL DP+S Sbjct: 121 AFAHKGGIHVSAILRDSRTYEHIEPEKVGNKQRVLVSELAGQSNIVSKAQDMGLDFDPAS 180 Query: 355 EQARKVIDKIKNLEHQGYQFEGADASLELLLREATGEMNELFTFESFKMLVEKTAGSPVV 414 EQ++ +I KIK+LEH+GYQFEGADASLELLLREA GE+ ELFTFESFKMLVEK+AG PVV Sbjct: 181 EQSKHIIGKIKDLEHEGYQFEGADASLELLLREANGELKELFTFESFKMLVEKSAGQPVV 240 Query: 415 SEAFVKLKVGGDNLYTAAEGNGPVNALDNALRKALLTYFPQLKDMHLSDYKVRVLDEKDQ 474 SEAFVK+ V G+++YTAAEGNGPVNALDNALRKAL+ YFP LK+MHL+DYKVRVLD+KD Sbjct: 241 SEAFVKVNVAGESIYTAAEGNGPVNALDNALRKALVQYFPTLKEMHLADYKVRVLDDKDA 300 Query: 475 TAAKVRVLIESKDYSDTWNTVGVSSNVIEASWEALVDSMRYALLGQISLENGTEISSGPR 534 TAAKVRVLIESK++ + WNTVGVSSNVIEASWEALVDSMRYALLGQIS E E S+ + Sbjct: 301 TAAKVRVLIESKNFENAWNTVGVSSNVIEASWEALVDSMRYALLGQISPEQVHE-SAARQ 359 Query: 535 GLVNH 539 GLVNH Sbjct: 360 GLVNH 364 >A5D4W4.1 A5D4W4_PELTS Isopropylmalate/homocitrate/citramalate synthases Length = 546 Score = 587 bits (1512), Expect = e-165, Method: Compositional matrix adjust. Identities = 288/527 (54%), Positives = 372/527 (70%), Gaps = 3/527 (0%) Query: 4 SISIFDTTLRDGTQGEGISXXXXXXXXXXXXXXXXGVHYIEGGIPGSNNKDIEFFKRVKE 63 S+ I+DTTLRDG Q EGIS G HYIEGG PGSN KD+EFFK+++ Sbjct: 3 SVKIYDTTLRDGAQAEGISFSVEDKIKIALRLDKMGFHYIEGGWPGSNPKDLEFFKKIRH 62 Query: 64 LHL-HAKITAFGSTRRKNSITEHDENLQRMIDAGVPAATLVGKSWDFHVHTALQTTLEEN 122 L HA++ AFGSTR+ + E+D +++ +ID+GV AT+ GKSWDFHV AL TLEEN Sbjct: 63 CPLRHARLAAFGSTRKAGAAAENDASVKAIIDSGVQVATIFGKSWDFHVFKALGVTLEEN 122 Query: 123 LAMIGESIAYLKRKGLEVIFDAEHFFDGYKNNPEYXXXXXXXXXXXXXDWLVMCDTNGGT 182 LAMI E++AYLK +GLEVI+DAEHFFDG K NP Y +V+CDTNGGT Sbjct: 123 LAMIRETVAYLKSRGLEVIYDAEHFFDGCKANPAYALETIRAAAESGASAVVLCDTNGGT 182 Query: 183 LPNEIQEIVSXXXXXXXXXXXXIHTHNDCELAVANTLSAISAGARQVQGTINGYGERCGN 242 LP E++E+V IH HND ELAVANTL+A+ AGA QVQGT+NGYGERCGN Sbjct: 183 LPAEVKELVEKARSVLGVPVG-IHAHNDGELAVANTLAAVQAGAEQVQGTVNGYGERCGN 241 Query: 243 ANLCSIIPTLQLKMGYHCIPGDSLPQLTNTAHYISEVANVNLPVNQPYVGNAAFAHKGGI 302 ANLCS+IP L LK G IP + L LT+ +H++SEVAN++ +QP+VG +AFAHKGG+ Sbjct: 242 ANLCSVIPNLTLKCGIETIPREKLVHLTDLSHFVSEVANISPNPHQPFVGASAFAHKGGV 301 Query: 303 HVSAILRDSRTYEHIAPELVGNKQRVLVSELAGQSNVLSKAQDMGLSLDPSSEQARKVID 362 HVSA+L+DSRTYEHI+P VGN++RVLVSEL+G SN+L K +++ L ++ S + +++++ Sbjct: 302 HVSALLKDSRTYEHISPGEVGNRRRVLVSELSGMSNLLYKYKELNLDIERQSPEGKRILE 361 Query: 363 KIKNLEHQGYQFEGADASLELLLREATGEMNELFTFESFKMLVEKTAGSPVVSEAFVKLK 422 IK LE QG+QFE A+ S ELL+R+ + F E+ ++LVE +P SEA +K++ Sbjct: 362 DIKKLESQGFQFEDAEGSFELLVRKTNHGYRDPFILEALRLLVEIKENTPAYSEAIIKMR 421 Query: 423 VGGDNLYTAAEGNGPVNALDNALRKALLTYFPQLKDMHLSDYKVRVLDEKDQTAAKVRVL 482 VG ++TAAEGNGPVNALDNALRKAL ++P + MHL+DYKVRVL+EKD T A VRV Sbjct: 422 VGDRVVHTAAEGNGPVNALDNALRKALYDFYPCIGSMHLTDYKVRVLEEKDGTGAAVRVH 481 Query: 483 IESKDYSDTWNTVGVSSNVIEASWEALVDSMRYALL-GQISLENGTE 528 I++ D +W TVGVS N+IEASW+AL DS+ Y LL G NG E Sbjct: 482 IQTGDGKRSWGTVGVSRNIIEASWQALADSISYGLLKGDHLARNGEE 528 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.315 0.132 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,923,898,925 Number of extensions: 114142872 Number of successful extensions: 284635 Number of sequences better than 1.0e-05: 2042 Number of HSP's gapped: 278702 Number of HSP's successfully gapped: 2050 Length of query: 539 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 398 Effective length of database: 1,732,333,088 Effective search space: 689468569024 Effective search space used: 689468569024 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.5 bits) S2: 134 (56.2 bits)