BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0345.0007.p (520 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KHK1.1 B3KHK1_9BACI Fmu (Sun) domain protein 590 e-166 B1DFI7.1 B1DFI7_9BACL Fmu (Sun) domain protein 434 e-119 C0ZI58.1 C0ZI58_BREBN Probable ribosomal RNA small subunit methy... 397 e-108 A1HRN9.1 A1HRN9_9FIRM Putative RNA methylase, NOL1/NOP2/sun family 366 4e-99 A4J9G7.1 A4J9G7_DESRM Fmu (Sun) domain protein 327 2e-87 >B3KHK1.1 B3KHK1_9BACI Fmu (Sun) domain protein Length = 534 Score = 590 bits (1520), Expect = e-166, Method: Compositional matrix adjust. Identities = 299/542 (55%), Positives = 358/542 (66%), Gaps = 37/542 (6%) Query: 6 MAVELPGTFMQRMEDMLGSDYEQFAESYQETPYGGIRANTLKIPVEELLALSPFTLAPIP 65 MA+ LP F+ +M+ +LG +Y+ F +SY++ P+ GIR NTLKI + L +SPF LAPIP Sbjct: 1 MALRLPSPFLAKMKSLLGENYDNFMKSYEQPPFAGIRVNTLKIGKADFLEISPFALAPIP 60 Query: 66 WCPSGFYTEAGARPGKHPYYHAGLYYIQEPSAMAPVELLGVRPGDRVLDLCAAPGGKSTQ 125 WCP+G+Y + G RPG+HPYYHAGLYYIQEPSAMAPVELL V PGDRVLDLCAAPGGKSTQ Sbjct: 61 WCPTGYYIQEGERPGRHPYYHAGLYYIQEPSAMAPVELLDVTPGDRVLDLCAAPGGKSTQ 120 Query: 126 IAAKLQGEGLLVSNDLHPDRTKALAKNLELYGVRNGIVLNESPGRIAAAFPGFFDRILID 185 IAAKL+G+G+L+SNDLHPDRTKALAKNLE+YGVRN +VLNE P RIA AFP FF +ILID Sbjct: 121 IAAKLKGQGMLISNDLHPDRTKALAKNLEMYGVRNAVVLNEEPERIAGAFPEFFTKILID 180 Query: 186 APCSGEGMFRKDEDMVKQWEPETPLKYAAMQREILASAAAALKPGGTLVYSTCTFAIEEN 245 APCSGEGMFRKDE M+K WE +PLK+A MQR+IL SAAA L PGG LVYSTCTF+ EEN Sbjct: 181 APCSGEGMFRKDESMLKYWEESSPLKFAEMQRDILKSAAAMLCPGGRLVYSTCTFSPEEN 240 Query: 246 EGIIGEFLASHPEFSLIPVGGTGSFXXXXXXXXXXXXXXX---------------HKVKG 290 EG+I EFL+ HP+F ++PV + F HKV+G Sbjct: 241 EGMIAEFLSEHPDFRVVPVDHSEFFSPGETDWMAGQGNVKDDVLGQLAGTARLWPHKVRG 300 Query: 291 EGHFMAVLRHDGXXXXXXXXXXGLKLKERDKA------------LPVSPRNKEXXXXXXX 338 EGHFMAVL+ +G + E+D+A L VSPR Sbjct: 301 EGHFMAVLQREGELDR--------REAEKDQAPWIQTGSLDLGSLAVSPRTDSSRERKGK 352 Query: 339 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXQSQDLGEEAALAAYSVFAEEQLGWQPAGYPV 398 Q +L + F +EQL + GYPV Sbjct: 353 SALREERPGKPGRGSNGASRQQAPRRSGGDRQS--NPISLDPFQAFIQEQLHVKLEGYPV 410 Query: 399 LFGDHLYLSPLPKAALDGLKTIRPGWYAGHVRSGRFIPGHPLATALHPAESIRSLSLPGN 458 ++G H+Y+SPLP+ L LK IRPGWY G +++ RF+PGHPLATAL E+ R +SL Sbjct: 411 VYGSHVYVSPLPEERLKQLKAIRPGWYMGQLKNERFVPGHPLATALQADEAARHVSLSSK 470 Query: 459 SGEAVSYLKGETLSIPAERLSVRSGSSPKGYVLVCIDGYSAGWGKWQDGMLKNEYPAGWR 518 GEAV YLKGETLSIP ER++ R G+ KGYVLVCIDGYSAGWGKWQ GMLKNEYPAGWR Sbjct: 471 DGEAVRYLKGETLSIPEERVTCRPGAEAKGYVLVCIDGYSAGWGKWQSGMLKNEYPAGWR 530 Query: 519 WT 520 WT Sbjct: 531 WT 532 >B1DFI7.1 B1DFI7_9BACL Fmu (Sun) domain protein Length = 534 Score = 434 bits (1116), Expect = e-119, Method: Compositional matrix adjust. Identities = 246/540 (45%), Positives = 306/540 (56%), Gaps = 33/540 (6%) Query: 6 MAVELPGTFMQRMEDMLGSDYEQFAESYQETPYGGIRANTLKIPVEELLALSPF--TLAP 63 M+ LP F +M+ +LG ++++F SY++ GIR N LK+ E ++SP + P Sbjct: 1 MSGYLPIAFTTKMKSLLGEEFDRFMSSYEDPRVYGIRINRLKLDAGEWKSISPMGEAVRP 60 Query: 64 IPWCPSGFYTEAGARPGKHPYYHAGLYYIQEPSAMAPVELLGVRPGDRVLDLCAAPGGKS 123 IPW P GFY E GARPGKHP+YHAGLYYIQEPSAMAPVELL V PG RVLDLCAAPGGKS Sbjct: 61 IPWAPEGFYYEEGARPGKHPHYHAGLYYIQEPSAMAPVELLDVHPGHRVLDLCAAPGGKS 120 Query: 124 TQIAAKLQGEGLLVSNDLHPDRTKALAKNLELYGVRNGIVLNESPGRIAAAFPGFFDRIL 183 TQIAAKLQG G+LVSND +RTKALAKN+EL GVRN +VLNE P +A F G+FDRIL Sbjct: 121 TQIAAKLQGSGVLVSNDNARERTKALAKNIELAGVRNAVVLNEEPSALAPVFRGWFDRIL 180 Query: 184 IDAPCSGEGMFRKDEDMVKQWEPETPLKYAAMQREILASAAAALKPGGTLVYSTCTFAIE 243 +DAPCSGEGMFRKDE M+ +WE + + + MQR IL AAA L PGG LVYSTCTF+ E Sbjct: 181 VDAPCSGEGMFRKDESMIAEWEKHSIERCSVMQRHILRDAAAMLAPGGVLVYSTCTFSPE 240 Query: 244 ENEGIIGEFLASHPEFSLIPV----------------GGTGSFXXXXXXXXXXXXXXXHK 287 ENE I E LA +PEF + PV G G H Sbjct: 241 ENEAQIAELLAEYPEFEVRPVRHRYGWKPGHPEWAGEAGEGLAPGQSESLAGTVRLWPHH 300 Query: 288 VKGEGHFMAVLRHDGXXXXXXXXXXGLKLKERDKALPVSPRNKEXXXXXXXXXXXXXXXX 347 ++GEGH+ AVL G K + + A+ V N + Sbjct: 301 IEGEGHYAAVLTRKGPAPAE-------KRQFDEPAVLVEEDNGKRKKVKGNAGAALRAAE 353 Query: 348 XXXXXXXXXXXXXXXXXXXXQSQDLGEEA---ALAAYSVFAEEQL--GWQPAGYPVLFGD 402 + G+ A A++ FAE++L G+ G FG Sbjct: 354 KKGVREAKDNVKLERAGGSARHSGKGQSGVVDAAASWLSFAEKELSGGFAGTGSVHSFGS 413 Query: 403 HLYLSPLPKAALDGLKTIRPGWYAGHVRSGRFIPGHPLATALHPAESIRSLSLPGNSGEA 462 +YL P +LDGL +R GWY G RF P LA L ++++RSL + + Sbjct: 414 RVYLQPEGMPSLDGLHVVRAGWYVGEAGRNRFEPSQALAMGLRQSDAVRSLDFASDDPDI 473 Query: 463 VSYLKGETLSIPAERLSVRSGSSP--KGYVLVCIDGYSAGWGKWQ-DGMLKNEYPAGWRW 519 + YLKGETL + A+ + + + KGYVLVC DGY GWGK+ DGMLKNE PAGWRW Sbjct: 474 IRYLKGETLFVEADHIKIEGAPAQPLKGYVLVCTDGYPVGWGKYTGDGMLKNELPAGWRW 533 >C0ZI58.1 C0ZI58_BREBN Probable ribosomal RNA small subunit methyltransferase EC=2.1.1.- Length = 470 Score = 397 bits (1019), Expect = e-108, Method: Compositional matrix adjust. Identities = 222/527 (42%), Positives = 293/527 (55%), Gaps = 71/527 (13%) Query: 6 MAVELPGTFMQRMEDMLGSDYEQFAESYQETPYGGIRANTLKIPVEELLALSPFTLAPIP 65 M LP F++RM+ +LG DYE F SY + G+R N LK+ ++ L +SPF + +P Sbjct: 1 MTKNLPTPFIERMKTLLGHDYEAFVSSYDQPVTHGLRVNPLKVERDDFLKISPFAVEAVP 60 Query: 66 WCPSGFYTEAGARPGKHPYYHAGLYYIQEPSAMAPVELLGVRPGDRVLDLCAAPGGKSTQ 125 WC +GF + ARPGKHP++ AG+YY+QEPSAM+ E LG +PG+R+LDLCAAPGGKSTQ Sbjct: 61 WCENGFRYKEPARPGKHPFHSAGMYYLQEPSAMSAAEALGAQPGERILDLCAAPGGKSTQ 120 Query: 126 IAAKLQGEGLLVSNDLHPDRTKALAKNLELYGVRNGIVLNESPGRIAAAFPGFFDRILID 185 +AA L G+G+LV+N++HP R KAL++NLE GV N +V NE+P R+ FP FFDRIL+D Sbjct: 121 LAAFLHGQGMLVANEIHPVRAKALSENLERCGVTNAVVTNETPERLQERFPQFFDRILVD 180 Query: 186 APCSGEGMFRKDEDMVKQWEPETPLKYAAMQREILASAAAALKPGGTLVYSTCTFAIEEN 245 APCSGEGMFRK + ++ W P + MQ +IL +AAA LKPGGTLVYSTCTFA EN Sbjct: 181 APCSGEGMFRKLPEAIEDWSPAKVTECHVMQGDILEAAAAMLKPGGTLVYSTCTFAPLEN 240 Query: 246 EGIIGEFLASHPEFSLIPVGGTGSFXX--------XXXXXXXXXXXXXHKVKGEGHFMAV 297 E + FL HPEF ++P+ F H+++GEGH++A Sbjct: 241 EQSLVNFLGRHPEFEIVPLPHADCFSAGQPEWASPQNEQLTQTARLWPHRLQGEGHYLA- 299 Query: 298 LRHDGXXXXXXXXXXGLKLKERDKALPVSPRNKEXXXXXXXXXXXXXXXXXXXXXXXXXX 357 KL++ + A P K Sbjct: 300 -----------------KLQKSETAEVHEPSGKR-------------------------- 316 Query: 358 XXXXXXXXXXQSQDLGEEAALAAYSVFAEEQL-----GWQPAGYPVLFGDHLYLSPLPKA 412 +S G + ALAA+ FA E L ++ +LFG+ LY SP P+ Sbjct: 317 ----KEKRAAKSAPAGRKEALAAWRTFAAEALPALSKPFEDESAFLLFGEQLYYSPAPEL 372 Query: 413 ALDGLKTIRPGWYAGHVRSGRFIPGHPLATALHPAESIRSLSLPGNSGEAVSYLKGETLS 472 A D LK R G + G V+ RF P H LA AL PAE+ R S + E + YLKGE L+ Sbjct: 373 AWDKLKVARVGLHLGTVKKNRFEPAHALALALSPAEAARVASYGADDPELLRYLKGEALT 432 Query: 473 IPAERLSVRSGSSPKGYVLVCIDGYSAGWGKWQDGMLKNEYPAGWRW 519 R G S G+ LV ++G+S GWGK +G LKN YP G RW Sbjct: 433 --------REGES--GWTLVTVEGFSIGWGKQSEGQLKNHYPKGLRW 469 >A1HRN9.1 A1HRN9_9FIRM Putative RNA methylase, NOL1/NOP2/sun family Length = 458 Score = 366 bits (939), Expect = 4e-99, Method: Compositional matrix adjust. Identities = 219/522 (41%), Positives = 283/522 (54%), Gaps = 73/522 (13%) Query: 6 MAVELPGTFMQRMEDMLGSDYEQFAESYQETPYGGIRANTLKIPVEELLALSPFTLAPIP 65 M +LP F QRM+ +L ++ F +SY+E G+R NTLKI EL++L+PF L P+P Sbjct: 1 MHHDLPAAFCQRMQALLADEFAAFYQSYEEPRTYGLRVNTLKITPAELVSLAPFALTPVP 60 Query: 66 WCPSGFYTEAGARPGKHPYYHAGLYYIQEPSAMAPVELLGVRPGDRVLDLCAAPGGKSTQ 125 W GFY RPGKHPY+ AGLYYIQEPSAMA LL +PG+ VLDL AAPGGK+T Sbjct: 61 WATDGFYCLEEQRPGKHPYHAAGLYYIQEPSAMAAAPLLAPQPGEFVLDLAAAPGGKATH 120 Query: 126 IAAKLQGEGLLVSNDLHPDRTKALAKNLELYGVRNGIVLNESPGRIAAAFPGFFDRILID 185 +AA +QG+GLL++N++HP R K L++N+E GV+N IV NE+P R+A FP FFDRIL+D Sbjct: 121 LAAFMQGQGLLIANEVHPARAKILSENIERLGVKNCIVTNETPPRLAERFPAFFDRILVD 180 Query: 186 APCSGEGMFRKDEDMVKQWEPETPLKYAAMQREILASAAAALKPGGTLVYSTCTFAIEEN 245 APCSGEGMFRKD ++W + P + AA QREILA A LK GG L+YSTCTFA EEN Sbjct: 181 APCSGEGMFRKDPAACQEWTQDAPRQCAARQREILADAVRMLKSGGWLLYSTCTFAPEEN 240 Query: 246 EGIIGEFLASHPEFSLIPVGGTGSFXXXX-------XXXXXXXXXXXHKVKGEGHFMAVL 298 E I+ LA++P+ +++P+ F HK++GEGHF+A+ Sbjct: 241 EEIVDWLLAAYPDLAVVPLPHNHGFASAVPAWGGGRSDLAGAVRLWPHKLRGEGHFLAL- 299 Query: 299 RHDGXXXXXXXXXXGLKLKERDKALPVSPRNKEXXXXXXXXXXXXXXXXXXXXXXXXXXX 358 L+ + K + P KE Sbjct: 300 ---------------LRKQGEGKGTGIRPGRKEGKRRA---------------------- 322 Query: 359 XXXXXXXXXQSQDLGEEAALAAYSVFAEEQLGWQPAGYPVLFGDHLYLSPLPKAALDGLK 418 ++ L Y F E P G LFGDHLYL P LDGLK Sbjct: 323 ----------------DSRLKDYLAFCREFFTTVPEGDLQLFGDHLYLLPPGAPPLDGLK 366 Query: 419 TIRPGWYAGHVRSGRFIPGHPLATALHPAESIRSLSLPGNSGEAV-SYLKGETLSIPAER 477 +RPGW+ G + RF P H LA A+ A+ +R + + +AV YL+GE L E Sbjct: 367 ILRPGWHLGWLAKNRFEPSHALAMAI-TAKDVRYTADYSAADDAVWRYLRGEALPRQGE- 424 Query: 478 LSVRSGSSPKGYVLVCIDGYSAGWGKWQDGMLKNEYPAGWRW 519 KG+ LV +DGY GWGK LKN YP G RW Sbjct: 425 ---------KGWTLVTVDGYPLGWGKQSADSLKNHYPKGLRW 457 >A4J9G7.1 A4J9G7_DESRM Fmu (Sun) domain protein Length = 470 Score = 327 bits (838), Expect = 2e-87, Method: Compositional matrix adjust. Identities = 163/299 (54%), Positives = 204/299 (68%), Gaps = 17/299 (5%) Query: 3 EGIMAVELPGTFMQRMEDMLGSDYEQFAESYQETPYGGIRANTLKIPVEELLALSPFTLA 62 +GI ++LP F+ +ME ++G+DY QF SY+ + G+RAN LKI +L L F Sbjct: 2 KGIEEMKLPAAFLSKMEQLVGADYPQFLRSYEGSRLAGLRANGLKISPSDLNELLSFLEE 61 Query: 63 PIPWCPSGFY-TEAGARPGKHPYYHAGLYYIQEPSAMAPVELLGVRPGDRVLDLCAAPGG 121 PI W GFY EA RP KHPYY+AGLYYIQEPSAM P ELLG +PG+RVLDLCAAPGG Sbjct: 62 PIAWSKDGFYYNEATTRPAKHPYYYAGLYYIQEPSAMLPAELLGAQPGERVLDLCAAPGG 121 Query: 122 KSTQIAAKLQGEGLLVSNDLHPDRTKALAKNLELYGVRNGIVLNESPGRIAAAFPGFFDR 181 KS Q+AA L +GLLV+NDLHP R + L KN+E YGV N IVLNE+P +A F GFFDR Sbjct: 122 KSLQLAAHLGRKGLLVTNDLHPQRARVLLKNIERYGVANAIVLNETPSHLAKVFAGFFDR 181 Query: 182 ILIDAPCSGEGMFRKDEDMVKQWEPETPLKYAAMQREILASAAAALKPGGTLVYSTCTFA 241 IL+DAPCSGEGMFRK+ DM K+W PE KYA Q+EIL + + L+PGG +VYSTCTF+ Sbjct: 182 ILVDAPCSGEGMFRKEPDMAKEWSPEEVNKYARWQQEILEAVPSLLRPGGEVVYSTCTFS 241 Query: 242 IEENEGIIGEFLASHPEFSLIPVGGTGSFXXXXXXXXXXXXXXXHKVKGEGHFMAVLRH 300 EENE + F+A +P+F L+ + H+VKGEGHF+A L++ Sbjct: 242 PEENEQQMQSFVAKYPDFELVEL----------------RRLWPHQVKGEGHFVAKLKN 284 Score = 70.5 bits (171), Expect = 4e-10, Method: Compositional matrix adjust. Identities = 53/157 (33%), Positives = 72/157 (45%), Gaps = 19/157 (12%) Query: 375 EAALAAYSV-FAEEQLGWQ---PAGYPVLFGD-HLYLSPLPKAALDGLKTIRPGWYAGHV 429 + ALA +S+ + GWQ P G V+ D H+ L L GLK +R GW G V Sbjct: 317 KQALADFSMQVWRNKKGWQEWLPLGGQVIERDGHILWESLDLPVLRGLKVLRSGWLLGTV 376 Query: 430 RSGRFIPGHPLATAL---HPAESIRSLSLPGNSGE----AVSYLKGETLSIPAERLSVRS 482 GRF P A L +++ L L S + YL+GET+ + Sbjct: 377 ERGRFRPSPAFALGLPMGAVKSAVQKLVLSAKSQRENSLVLRYLRGETIQQEGK------ 430 Query: 483 GSSPKGYVLVCIDGYSAGWGKWQDGMLKNEYPAGWRW 519 + KG+ LVC+D + GW K LKN +P GWRW Sbjct: 431 -TWEKGWHLVCLDEFPLGWAKGDGNWLKNGFPPGWRW 466 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.137 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,040,770,210 Number of extensions: 129726766 Number of successful extensions: 298083 Number of sequences better than 1.0e-05: 2668 Number of HSP's gapped: 293673 Number of HSP's successfully gapped: 2809 Length of query: 520 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 380 Effective length of database: 1,741,754,103 Effective search space: 661866559140 Effective search space used: 661866559140 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 134 (56.2 bits)