BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0345.0005.p (266 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KHJ9.1 B3KHJ9_9BACI Cof-like hydrolase 342 2e-92 B1DFI5.1 B1DFI5_9BACL Cof-like hydrolase 277 6e-73 B6EQW3.1 B6EQW3_ALISL Phosphatase EC=3.1.3.- 149 2e-34 C1PD30.1 C1PD30_BACCO Cof-like hydrolase 149 3e-34 Q8YA87.1 Q8YA87_LISMO Lmo0272 protein 147 6e-34 >B3KHJ9.1 B3KHJ9_9BACI Cof-like hydrolase Length = 266 Score = 342 bits (877), Expect = 2e-92, Method: Compositional matrix adjust. Identities = 156/266 (58%), Positives = 202/266 (75%) Query: 1 MKYKLIALDVDGTLLNDDHKLSPENREAIAEVTRQGGLIVLCTGRSPQNSIPLMEEMGLA 60 M YKL+ALDVDGTLLND H+++ + R + E +QG IVLCTGR P NSIP M+ MGL Sbjct: 1 MGYKLVALDVDGTLLNDHHEITEKTRNTVMEAAKQGVEIVLCTGRGPLNSIPFMKSMGLG 60 Query: 61 GYVLGHNGAANVRVEDREVLHIYGLDARGLDPYIDYCRARDIHYDVNTAFDMYVDNVANL 120 GYV+ HNGAA V VE ++H +G+D L+P++ YCR +H+DVNTAF+MYVD V L Sbjct: 61 GYVISHNGAATVEVETHHIVHQFGMDPGQLEPFMKYCREHGVHFDVNTAFEMYVDQVDEL 120 Query: 121 TQEATYMYEHFRITPASLPAWEDFREPVVKFTVFTKADILDEAQREWSTWTQQYNIVRSG 180 MYE++ + P++LP+WE+ +PVVKFTVF ++ +D +WS WT +N++RSG Sbjct: 121 AGPVRSMYENYLMLPSNLPSWEELSDPVVKFTVFGESADIDRVYADWSKWTLPFNMLRSG 180 Query: 181 EFFVDFMHPEASKGNALMNLASQLGIAQEEVLAIGNYYNDISMLTYAGLGIAMDNSPLEV 240 EFF+D MH +ASKGNAL LA++ G +EVLAIGNY+NDI+MLTYAG+GIAMDNSP+EV Sbjct: 181 EFFIDLMHSQASKGNALKQLAAKRGYRPDEVLAIGNYFNDITMLTYAGMGIAMDNSPVEV 240 Query: 241 KAAADAVTGSNNDHGVRDALVKYCLS 266 KAAA+AVT SNN+ GV +ALVKYCLS Sbjct: 241 KAAANAVTASNNEDGVHEALVKYCLS 266 >B1DFI5.1 B1DFI5_9BACL Cof-like hydrolase Length = 265 Score = 277 bits (709), Expect = 6e-73, Method: Compositional matrix adjust. Identities = 133/266 (50%), Positives = 177/266 (66%), Gaps = 3/266 (1%) Query: 1 MKYKLIALDVDGTLLNDDHKLSPENREAIAEVTRQGGLIVLCTGRSPQNSIPLMEEMGLA 60 ++Y LIALDVDGTLL DDH+L+ + REA+ E +G IVLCTGR+P + P++EE+GL Sbjct: 3 LQYDLIALDVDGTLLTDDHQLTSKTREAVREAADRGASIVLCTGRAPFSVFPVLEELGLT 62 Query: 61 GYVLGHNGAANVRVEDREVLHIYGLDARGLDPYIDYCRARDIHYDVNTAFDMYVDNVANL 120 G ++ HNG A + + R ++H YG+ + L+P++DYCR +HYDVNTAF+M V++ L Sbjct: 63 GTIITHNGGATIDSDTRRIIHEYGIAPQQLEPFVDYCRKNQVHYDVNTAFEMMVES---L 119 Query: 121 TQEATYMYEHFRITPASLPAWEDFREPVVKFTVFTKADILDEAQREWSTWTQQYNIVRSG 180 T E + MY F +P+ L E +VKFTVF +++D Q EW W + +RSG Sbjct: 120 TPEVSDMYSKFHASPSVLGQGASLPEGLVKFTVFGSKEVMDRVQEEWEKWPPSLHTIRSG 179 Query: 181 EFFVDFMHPEASKGNALMNLASQLGIAQEEVLAIGNYYNDISMLTYAGLGIAMDNSPLEV 240 +FF+D HPEASKG AL AS GI + +LAIGNYYNDISML +AG GIAM NSP V Sbjct: 180 DFFIDVHHPEASKGRALRQFASSRGIDRSRILAIGNYYNDISMLEFAGCGIAMGNSPDGV 239 Query: 241 KAAADAVTGSNNDHGVRDALVKYCLS 266 K AAD V SN + GV AL ++ S Sbjct: 240 KDAADVVGRSNAEDGVAYALREFAWS 265 >B6EQW3.1 B6EQW3_ALISL Phosphatase EC=3.1.3.- Length = 269 Score = 149 bits (377), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 96/277 (34%), Positives = 155/277 (55%), Gaps = 23/277 (8%) Query: 3 YKLIALDVDGTLLNDDHKLSPENREAIAEVTRQGGLIVLCTGRSPQNSIPLMEEMGLAG- 61 YKLIALD+DGTLLN D ++SPEN+ AIA QG ++VL +GR ++E+G+ Sbjct: 2 YKLIALDMDGTLLNSDKQISPENKAAIAAAREQGVVVVLASGRPLNGMKSQLKELGMTSD 61 Query: 62 --YVLGHNGAANVRVEDREVLH---IYGLDARGLDPYIDYCRARDIHYDVNTAFDMYVDN 116 YVL +N + RV+ E++ I G DA+ L A+++ ++ + Sbjct: 62 EDYVLSYNASLVQRVKSEEIIRSQIITGKDAKVLAKL-----AKELGVHIHA----FSQE 112 Query: 117 VANLTQEATYMYEH------FRITPASLPAWEDFREPVVKFTVFTKADILDEAQREW-ST 169 +T E++Y EH IT + A D E ++K + A++L A ++ Sbjct: 113 KGLITPESSYYTEHESTITGMPITVINFEALAD-DEQILKAMMIDDAELLSVAIKQLPEA 171 Query: 170 WTQQYNIVRSGEFFVDFMHPEASKGNALMNLASQLGIAQEEVLAIGNYYNDISMLTYAGL 229 +Q+ IV+S FF++F++P ++KG + LA LGI +EV+ +G+ ND M+ YAGL Sbjct: 172 LYKQFTIVQSAPFFLEFLNPNSNKGVGVKALADHLGITADEVICMGDAGNDWHMIKYAGL 231 Query: 230 GIAMDNSPLEVKAAADAVTGSNNDHGVRDALVKYCLS 266 G+AM N+ +VK A+ +T SNN+HGV + ++ L+ Sbjct: 232 GVAMANATDDVKEIANHITVSNNEHGVAKVIEEFVLN 268 >C1PD30.1 C1PD30_BACCO Cof-like hydrolase Length = 269 Score = 149 bits (375), Expect = 3e-34, Method: Compositional matrix adjust. Identities = 111/286 (38%), Positives = 153/286 (53%), Gaps = 40/286 (13%) Query: 3 YKLIALDVDGTLLNDDHKLSPENREAIAEVTRQGGLIVLCTGRSPQNSIPLMEEMGLA-- 60 YKLIA+D+DGTLLND H+++PE REA+ + ++G IVLCTGR +E++ L Sbjct: 2 YKLIAIDMDGTLLNDQHEVTPEVREALHKAKKKGVKIVLCTGRPIGGVRRFLEDLLLNEE 61 Query: 61 -GYVLGHNGA------ANVRVEDREV-----LHIYGLD------ARGLDPYIDYCRARDI 102 YV+ +NGA N V +R + + +YGL DP Y RD+ Sbjct: 62 DDYVIAYNGALVQNTHTNGVVAERSIGYGDLVRLYGLSLELKTPMHFFDPEHLYTPNRDL 121 Query: 103 H-YDVNTAFDMYVDNVANLTQEATYMYEHFRITPASLPAWEDFREPVVKFTVFTKADILD 161 Y V ++ LTQ M H+R T LP D P V F + + L Sbjct: 122 SPYTVVESY---------LTQ----MPVHYR-TLEELP--RDMVIPKVMF--IDEPEKLS 163 Query: 162 EAQREWSTWTQQ-YNIVRSGEFFVDFMHPEASKGNALMNLASQLGIAQEEVLAIGNYYND 220 W Q+ Y +VRS F+ + +H EASKGNA+ LA LGI +EEV+ IG+ ND Sbjct: 164 RTIEAIPDWVQEKYTMVRSMPFYYEILHKEASKGNAVKLLAGHLGIHREEVVCIGDNEND 223 Query: 221 ISMLTYAGLGIAMDNSPLEVKAAADAVTGSNNDHGVRDALVKYCLS 266 ++M+ YAG G+AM N+ +VKA AD T +NN+HGV A+ K LS Sbjct: 224 LTMIEYAGCGVAMGNAVPKVKAIADFETRTNNEHGVAYAIEKLVLS 269 >Q8YA87.1 Q8YA87_LISMO Lmo0272 protein Length = 270 Score = 147 bits (372), Expect = 6e-34, Method: Compositional matrix adjust. Identities = 99/282 (35%), Positives = 148/282 (52%), Gaps = 34/282 (12%) Query: 3 YKLIALDVDGTLLNDDHKLSPENREAIAEVTRQGGLIVLCTGR---SPQNSIPLMEEMGL 59 YKLIA+D+DGTLL DDHK++ E +EAI + +G +VLCTGR +N + +E Sbjct: 2 YKLIAIDIDGTLLTDDHKVTDEVKEAIRQAKLKGVKVVLCTGRPLVGVENYLTELELREE 61 Query: 60 AGYVLGHNGAANVRVEDREVLHIYGLDARGLDPYIDYCRARDIH---------YDVNTAF 110 YV+ NGA +EV+ L L + ++H Y N Sbjct: 62 GDYVISFNGAFVQDTFTKEVISHLTLGIEDLKDIYEVSLNSNLHMHFFDDKALYTPNREI 121 Query: 111 DMYVDNVANLTQEATYMYEHFRITPASLPAWEDFREPVVKFTVFTKADILDEA------- 163 Y A LT + +Y+ P EDF + +KA ++EA Sbjct: 122 GKYTIVEAYLTG-SQLIYKEIENVP------EDF--------IMSKAMFIEEAPELEVGI 166 Query: 164 QREWSTWTQQYNIVRSGEFFVDFMHPEASKGNALMNLASQLGIAQEEVLAIGNYYNDISM 223 + ++ ++Y++VRS F+++ ++ +ASKGNA+ L+ +LGI Q EV+ IG+ ND++M Sbjct: 167 AKIPESFREKYHLVRSTPFYLEILNRDASKGNAVKELSEKLGIKQSEVICIGDQENDVTM 226 Query: 224 LTYAGLGIAMDNSPLEVKAAADAVTGSNNDHGVRDALVKYCL 265 L +AGLGIAM N+P +K AD T SNND GV A+ K+ L Sbjct: 227 LEFAGLGIAMGNAPERIKQLADYTTASNNDSGVAKAIQKFVL 268 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,624,534,962 Number of extensions: 65853955 Number of successful extensions: 177399 Number of sequences better than 1.0e-05: 3227 Number of HSP's gapped: 175210 Number of HSP's successfully gapped: 3388 Length of query: 266 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 133 Effective length of database: 1,807,701,208 Effective search space: 240424260664 Effective search space used: 240424260664 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)