BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0345.0004.p (464 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KHI8.1 B3KHI8_9BACI Fmu (Sun) domain protein 577 e-163 B1DFI3.1 B1DFI3_9BACL Fmu (Sun) domain protein 513 e-143 C0ZI58.1 C0ZI58_BREBN Probable ribosomal RNA small subunit methy... 408 e-112 Q5KZ34.1 Q5KZ34_GEOKA Hypothetical conserved protein 403 e-110 C5DAS2.1 C5DAS2_9BACI RNA methylase, NOL1/NOP2/sun family 402 e-110 >B3KHI8.1 B3KHI8_9BACI Fmu (Sun) domain protein Length = 462 Score = 577 bits (1488), Expect = e-163, Method: Compositional matrix adjust. Identities = 289/461 (62%), Positives = 337/461 (73%), Gaps = 8/461 (1%) Query: 1 MNEERLPAAYTAAIREMLGQEADAFLESYTARRTQGLRFNTLKSNSLSGRAAAEHTAVRF 60 M +E LP + +I E+LG + F +SY + RT GLR N LK+ + + + F Sbjct: 1 MGKELLPVGFVTSINEILGDASCDFWDSYDSPRTHGLRLNRLKA--VHAADSVKKLIRDF 58 Query: 61 DLTPVPWCPSGYYYEEPVRPGRHAYHTAGLYYIQEPSAMSAAELLAPRPGETVLDLAAAP 120 LT V WC +GYYY+E VRPG+H YH AGLYYIQEPSAMSA ELL P+PGET+LDLAAAP Sbjct: 59 RLTSVSWCDTGYYYDESVRPGKHPYHAAGLYYIQEPSAMSAVELLNPQPGETILDLAAAP 118 Query: 121 GGKTSHIAALMQGQGLLVSNEIHPERAKILAENVERLGISHAVVTSAAPGELSRRFPEVF 180 GGKTSHIA+ M G+GLLVSNEIHPERA+ILAENVER+GI++ VVTSAAP +LSRRF F Sbjct: 119 GGKTSHIASKMMGRGLLVSNEIHPERARILAENVERMGITNTVVTSAAPDQLSRRFVACF 178 Query: 181 DRIMLDAPCSGEGMFRKDPAAIGEWSPEHVEMCAARQWDILQDAYIMLKPGGSLAYSTCT 240 DRIMLDAPCSGEGMFRKD AAI EWSPEHV++C ARQWDI+Q+AY MLKPGG+LAYSTCT Sbjct: 179 DRIMLDAPCSGEGMFRKDAAAIAEWSPEHVDLCVARQWDIVQEAYRMLKPGGTLAYSTCT 238 Query: 241 FNRKENEETISRFLDHYPDMERIAEKRLWPHLEKGEGHFVALLRKAASEEEAPGRNKRGS 300 FN ENEE I L YPD+E KRLWPHLE+GEGHFVA+L K + E + + Sbjct: 239 FNTAENEEMIEHILSQYPDLELQQMKRLWPHLERGEGHFVAILVKTPAAEAYSDDQETFT 298 Query: 301 GRGKTSHK-----LPANASDAYQQFMSWAADELPGFA-GQGVPLLFGEALYLLPEAFGAS 354 GK K A A A F WA +LPGF G G P+LFGE LY LP + Sbjct: 299 NSGKAQAKSNRGGKQAPALSALADFQDWAKQDLPGFELGGGTPILFGEELYWLPVSESVP 358 Query: 355 LHTSLLEGLKVPRAGLHIAHLKKNRIEPAHALAMALQPEQAARSYNLAADSQDIQAWLRG 414 H L+GLK+PRAGLH+AHLKKNRIEPAHALAM+L P QAARS++L +DS + A+LRG Sbjct: 359 WHDKQLKGLKMPRAGLHLAHLKKNRIEPAHALAMSLNPGQAARSWDLPSDSAETAAYLRG 418 Query: 415 ESLPVPLELHGWTLVTVDGLPVSWGKASSGQLKNHLPKGLR 455 E L +P E GWTLVT DGLP+ WGKAS GQLKNHLPKGLR Sbjct: 419 EVLSIPSEYRGWTLVTTDGLPLGWGKASGGQLKNHLPKGLR 459 >B1DFI3.1 B1DFI3_9BACL Fmu (Sun) domain protein Length = 464 Score = 513 bits (1321), Expect = e-143, Method: Compositional matrix adjust. Identities = 271/468 (57%), Positives = 327/468 (69%), Gaps = 20/468 (4%) Query: 1 MNEERLPAAYTAAIREMLG-QEADAFLESYTARRTQGLRFNTLKSNSLSGRAAAEHTAVR 59 M +LP AY ++ +LG +EA+ FL+SY+ R GLRFN LK G AA Sbjct: 1 MKNVQLPEAYIRQMQALLGREEAERFLDSYSEARAYGLRFNPLKCRQPIGDNAAAALKQP 60 Query: 60 FDLTPVPWCPSGYYYEEPVRPGRHAYHTAGLYYIQEPSAMSAAELLAPRPGETVLDLAAA 119 F L PVPWC +GYYYEE RPG+H YHTAGLYYIQEPSAMSAAELL PGET+LD AAA Sbjct: 61 FHLEPVPWCNTGYYYEEAARPGKHPYHTAGLYYIQEPSAMSAAELLGAEPGETILDFAAA 120 Query: 120 PGGKTSHIAALMQGQGLLVSNEIHPERAKILAENVERLGISHAVVTSAAPGELSRRFPEV 179 PGGKT+ IA M G+GLL++NEIHP RAKIL+ENVERLGI +AVVT+AAP ELSRRFP Sbjct: 121 PGGKTTQIAGSMNGEGLLIANEIHPARAKILSENVERLGIRNAVVTNAAPHELSRRFPAF 180 Query: 180 FDRIMLDAPCSGEGMFRKDPAAIGEWSPEHVEMCAARQWDILQDAYIMLKPGGSLAYSTC 239 FDRIMLD+PCSGEGMFRKD A+ EWS HV CA R +IL+ A IMLKPGG L YSTC Sbjct: 181 FDRIMLDSPCSGEGMFRKDEDAVAEWSEAHVTYCAERSSEILEHAAIMLKPGGVLVYSTC 240 Query: 240 TFNRKENEETISRFLDHYPDMERIAEKRLWPHLEKGEGHFVALLRKA------ASEEEAP 293 TFNR ENE+TI RF+ +P + +RLWPH +GEGHFVA LRKA A+E++ P Sbjct: 241 TFNRAENEDTIERFVAGHPSFQVEQIERLWPHQVRGEGHFVARLRKASESEQLAAEDDLP 300 Query: 294 G-----RNKRGSGRGKTSHKLPANASDAYQQFMSWAADELPGFA-GQGVPLLFGEALYLL 347 R+KR +GR K P A F S+A LPGFA G G PL FG+ALY L Sbjct: 301 KSKNKRRDKRTAGRSK-----PLTAE--MDLFHSFAEQALPGFALGPGEPLRFGDALYWL 353 Query: 348 PEAFGASLHTSLLEGLKVPRAGLHIAHLKKNRIEPAHALAMALQPEQAARSYNLAADSQD 407 P S ++ L+GLKV R GLH+ ++KNR+EPAHALA+A+Q E A+ + + D+ + Sbjct: 354 PHQTSGSFGSADLDGLKVARPGLHLGDIRKNRLEPAHALALAVQAEDASWAQSYPPDAPE 413 Query: 408 IQAWLRGESLPVPLELHGWTLVTVDGLPVSWGKASSGQLKNHLPKGLR 455 + A+LRGE+LP ++ GW LV VDGLP+ W K+S GQLKNHLPKGLR Sbjct: 414 LAAYLRGETLPAEGDISGWGLVAVDGLPLGWAKSSGGQLKNHLPKGLR 461 >C0ZI58.1 C0ZI58_BREBN Probable ribosomal RNA small subunit methyltransferase EC=2.1.1.- Length = 470 Score = 408 bits (1048), Expect = e-112, Method: Compositional matrix adjust. Identities = 223/482 (46%), Positives = 293/482 (60%), Gaps = 42/482 (8%) Query: 4 ERLPAAYTAAIREMLGQEADAFLESYTARRTQGLRFNTLKSNSLSGRAAAEHTAVRFDLT 63 + LP + ++ +LG + +AF+ SY T GLR N LK + F + Sbjct: 3 KNLPTPFIERMKTLLGHDYEAFVSSYDQPVTHGLRVNPLKVERDDFLKISP-----FAVE 57 Query: 64 PVPWCPSGYYYEEPVRPGRHAYHTAGLYYIQEPSAMSAAELLAPRPGETVLDLAAAPGGK 123 VPWC +G+ Y+EP RPG+H +H+AG+YY+QEPSAMSAAE L +PGE +LDL AAPGGK Sbjct: 58 AVPWCENGFRYKEPARPGKHPFHSAGMYYLQEPSAMSAAEALGAQPGERILDLCAAPGGK 117 Query: 124 TSHIAALMQGQGLLVSNEIHPERAKILAENVERLGISHAVVTSAAPGELSRRFPEVFDRI 183 ++ +AA + GQG+LV+NEIHP RAK L+EN+ER G+++AVVT+ P L RFP+ FDRI Sbjct: 118 STQLAAFLHGQGMLVANEIHPVRAKALSENLERCGVTNAVVTNETPERLQERFPQFFDRI 177 Query: 184 MLDAPCSGEGMFRKDPAAIGEWSPEHVEMCAARQWDILQDAYIMLKPGGSLAYSTCTFNR 243 ++DAPCSGEGMFRK P AI +WSP V C Q DIL+ A MLKPGG+L YSTCTF Sbjct: 178 LVDAPCSGEGMFRKLPEAIEDWSPAKVTECHVMQGDILEAAAAMLKPGGTLVYSTCTFAP 237 Query: 244 KENEETISRFLDHYPDMERI------------------------AEKRLWPHLEKGEGHF 279 ENE+++ FL +P+ E + RLWPH +GEGH+ Sbjct: 238 LENEQSLVNFLGRHPEFEIVPLPHADCFSAGQPEWASPQNEQLTQTARLWPHRLQGEGHY 297 Query: 280 VALLRKAASEEEAPGRNKRGSGRGKTSHKLPANASDAYQQFMSWAADELPG----FAGQG 335 +A L+K+ + E KR R S PA +A + ++AA+ LP F + Sbjct: 298 LAKLQKSETAEVHEPSGKRKEKRAAKS--APAGRKEALAAWRTFAAEALPALSKPFEDES 355 Query: 336 VPLLFGEALYLLPEAFGASLHTSLLEGLKVPRAGLHIAHLKKNRIEPAHALAMALQPEQA 395 LLFGE LY P A + LKV R GLH+ +KKNR EPAHALA+AL P +A Sbjct: 356 AFLLFGEQLYYSPAPELA------WDKLKVARVGLHLGTVKKNRFEPAHALALALSPAEA 409 Query: 396 ARSYNLAADSQDIQAWLRGESLPVPLELHGWTLVTVDGLPVSWGKASSGQLKNHLPKGLR 455 AR + AD ++ +L+GE+L E GWTLVTV+G + WGK S GQLKNH PKGLR Sbjct: 410 ARVASYGADDPELLRYLKGEALTREGE-SGWTLVTVEGFSIGWGKQSEGQLKNHYPKGLR 468 Query: 456 II 457 + Sbjct: 469 WL 470 >Q5KZ34.1 Q5KZ34_GEOKA Hypothetical conserved protein Length = 454 Score = 403 bits (1035), Expect = e-110, Method: Compositional matrix adjust. Identities = 221/480 (46%), Positives = 287/480 (59%), Gaps = 54/480 (11%) Query: 5 RLPAAYTAAIREMLGQEADAFLESYTARRTQGLRFNTLKSNSLSGRAAAEHTAVRFDLTP 64 RLP A+ A ++++L +EAD F Y + GLR N LK++ A F L+P Sbjct: 2 RLPEAFVAKMKKLLDEEADEFFAVYENEKINGLRVNPLKTDP-----GAWAKTAPFSLSP 56 Query: 65 VPWCPSGYYYEEPVRPGRHAYHTAGLYYIQEPSAMSAAELLAPRPGETVLDLAAAPGGKT 124 VP+CP+G+YYE +PG+H YH AGLYYIQEPSAM+ AE L P PGE VLDL AAPGGKT Sbjct: 57 VPFCPTGFYYEPDEQPGKHPYHAAGLYYIQEPSAMAVAEALRPAPGERVLDLCAAPGGKT 116 Query: 125 SHIAALMQGQGLLVSNEIHPERAKILAENVERLGISHAVVTSAAPGELSRRFPEVFDRIM 184 + + A+M+ +GLLV+NEIHP+R K LAENVER G+++ VV + P L+ +FP FD+I+ Sbjct: 117 TQLGAMMENKGLLVANEIHPKRVKALAENVERFGLTNTVVVNETPEALAEQFPGFFDKIL 176 Query: 185 LDAPCSGEGMFRKDPAAIGEWSPEHVEMCAARQWDILQDAYIMLKPGGSLAYSTCTFNRK 244 +DAPCSGEGMFRKD A WSP +VE CAARQ IL+ AY MLK GG L YSTCTF+ + Sbjct: 177 VDAPCSGEGMFRKDEEAASFWSPAYVEECAARQRRILESAYAMLKEGGILVYSTCTFSPE 236 Query: 245 ENEETISRFLDHYPDM------------------------ERIAEKRLWPHLEKGEGHFV 280 ENE+TI L+ Y D+ + + RLWPH KGEGHFV Sbjct: 237 ENEQTIEWLLETYDDLRLLPIAKAGGLEPGRPEWTKTNRSDLVHAARLWPHRLKGEGHFV 296 Query: 281 ALLRKAASEEEAPGRNKRGSGRGKTSHKLPANASDAYQQFMS---WAADELPGFAGQGVP 337 A L+K S GR + + P A Y+QF W + G Sbjct: 297 AKLQKQRSTPPWQGRRAKANA--------PKAAIRLYRQFEQEALWTERD-------GTF 341 Query: 338 LLFGEALYLLPEAFGASLHTSLLEGLKVPRAGLHIAHLKKNRIEPAHALAMALQPEQAAR 397 + FG L +LPE L GLK+ RAGLH+ +KK R EP HALA+AL+ E+A Sbjct: 342 VSFGAHLTVLPE------RCPDLSGLKIVRAGLHLGDVKKERFEPNHALALALRTEEAKH 395 Query: 398 SYNLAADSQDIQAWLRGESLPVPLELHGWTLVTVDGLPVSWGKASSGQLKNHLPKGLRII 457 +L++ S++I + RGE+L + GW LV VDG P +WGK G +KN PKGLR++ Sbjct: 396 VLDLSSASREIVQYWRGETLSTGGD-RGWLLVAVDGFPFAWGKEVKGTVKNFYPKGLRLV 454 >C5DAS2.1 C5DAS2_9BACI RNA methylase, NOL1/NOP2/sun family Length = 454 Score = 402 bits (1034), Expect = e-110, Method: Compositional matrix adjust. Identities = 217/476 (45%), Positives = 288/476 (60%), Gaps = 48/476 (10%) Query: 5 RLPAAYTAAIREMLGQEADAFLESYTARRTQGLRFNTLKSNSLSGRAAAEHTAVRFDLTP 64 +LP+ + + +L EA F +Y + GLRFN LK + + T V F L+P Sbjct: 2 KLPSEFIEKMERLLEDEASRFFSTYHEEKANGLRFNPLKIDRETFL-----TLVPFALSP 56 Query: 65 VPWCPSGYYYEEPVRPGRHAYHTAGLYYIQEPSAMSAAELLAPRPGETVLDLAAAPGGKT 124 VP+CP+G+YY+ +PG+H YH AGLYYIQEPSAM AE+L P PGE VLDL AAPGGKT Sbjct: 57 VPFCPTGFYYDAHEQPGKHPYHAAGLYYIQEPSAMFVAEVLKPNPGEFVLDLCAAPGGKT 116 Query: 125 SHIAALMQGQGLLVSNEIHPERAKILAENVERLGISHAVVTSAAPGELSRRFPEVFDRIM 184 + +AA+M+ QGL+++NEIHP+R K L+EN+ER GI++A+VT+ P +L++ FP FD+I+ Sbjct: 117 TQLAAMMKNQGLIIANEIHPKRVKALSENIERFGITNALVTNETPEKLAKYFPGFFDKIL 176 Query: 185 LDAPCSGEGMFRKDPAAIGEWSPEHVEMCAARQWDILQDAYIMLKPGGSLAYSTCTFNRK 244 +DAPCSGEGMFRKD A+ WS HVE CA +Q IL AY MLK GG L YSTCTF+ + Sbjct: 177 VDAPCSGEGMFRKDEEAVQFWSQAHVEQCAIKQRHILDCAYEMLKEGGILVYSTCTFSPE 236 Query: 245 ENEETISRFLDHYPDMERIA-EK-----------------------RLWPHLEKGEGHFV 280 ENE+TI FL Y D+E ++ EK RLWPH KGEGHFV Sbjct: 237 ENEQTIEAFLQTYDDLELLSIEKVHGIQPGRREWTNTNFEEMERTARLWPHSLKGEGHFV 296 Query: 281 ALLRKAASEEEAPGRNKRGSGRGKTSHKLPANASDAYQQFMSWAADELPGFAGQGVPLLF 340 A ++K GR + + + K ++Q + E P +A F Sbjct: 297 AKIKKTGPSPSWNGRYAKPN-----ASKQMVREYRQFEQEVLQTEIEKPMYA-------F 344 Query: 341 GEALYLLPEAFGASLHTSLLEGLKVPRAGLHIAHLKKNRIEPAHALAMALQPEQAARSYN 400 L+ LP+ H +GLKV RAGLH+ KK R EP HALA++L+P+ S + Sbjct: 345 QHHLFALPD------HCPNFDGLKVVRAGLHLGEAKKQRFEPNHALALSLKPQDVRYSLD 398 Query: 401 LAADSQDIQAWLRGESLPVPLELHGWTLVTVDGLPVSWGKASSGQLKNHLPKGLRI 456 L++DS + +LRGE++ E GW LVTVDG P+ WGK G +KN PKGLRI Sbjct: 399 LSSDSVECLKYLRGETIQTG-EDRGWLLVTVDGYPLGWGKEVKGMVKNFYPKGLRI 453 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.133 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,945,132,799 Number of extensions: 130890253 Number of successful extensions: 350450 Number of sequences better than 1.0e-05: 2671 Number of HSP's gapped: 345090 Number of HSP's successfully gapped: 2699 Length of query: 464 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 325 Effective length of database: 1,751,175,118 Effective search space: 569131913350 Effective search space used: 569131913350 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)