BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0345.0002.p (508 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KHI5.1 B3KHI5_9BACI Metallophosphoesterase 459 e-127 B1DBL9.1 B1DBL9_9BACL Metallophosphoesterase 321 1e-85 Q8TNC7.1 Q8TNC7_METAC Phosphoesterase 287 2e-75 Q46GD4.1 Q46GD4_METBF Phosphoesterase 285 9e-75 Q12WJ5.1 Q12WJ5_METBU Metallophosphoesterase 279 5e-73 >B3KHI5.1 B3KHI5_9BACI Metallophosphoesterase Length = 435 Score = 459 bits (1180), Expect = e-127, Method: Compositional matrix adjust. Identities = 222/425 (52%), Positives = 299/425 (70%), Gaps = 2/425 (0%) Query: 1 MIPFRFLHAADLHLDSRFAGLAQLPQAIRSYLRESTFAALGRLVSVAVQEKTDFVVISGD 60 M FRF+HAADLHLDS F G++ L +RS+++ESTF AL RLV +A+ E+ DF+VISGD Sbjct: 1 MASFRFIHAADLHLDSPFIGISGLDDDLRSFVQESTFRALERLVELAIGEQVDFIVISGD 60 Query: 61 VYDISDASLQGQLRFQEALQELGKHGIAVFLIHGNHDPLDGPRLSTEPPGHVTVFGGSEP 120 VYD S+ SL+ QLRF ++L LG+ GIAV++IHGNHDPLD +LS P HV VFG +EP Sbjct: 61 VYDSSNISLRAQLRFLDSLNRLGREGIAVYVIHGNHDPLDSAKLSMTLPSHVHVFG-AEP 119 Query: 121 GQAVARRRSDGREVAVVSGISYPTAKVTENTALQFKRRPGSDLYHIALLHGNVDGDPQHE 180 A RRSD +EVAV++G+SYPT+KVT+N A+++ P LYHI LLH NVDGDPQHE Sbjct: 120 AAVTAVRRSDQQEVAVIAGMSYPTSKVTDNIAIRYPA-PSPGLYHIGLLHANVDGDPQHE 178 Query: 181 TYSPCSRKDLIGRGYDYWALGHIHKRRVLHEQPPIVYPGNIQGRSVKETGPKGCYVVDVN 240 TY+PC++K+L+ G+ YWALGHIH RR L E P IVYPGNIQGR V+ETGPKGCYVVDV+ Sbjct: 179 TYAPCTKKELVQAGFHYWALGHIHSRRTLQESPYIVYPGNIQGRHVRETGPKGCYVVDVD 238 Query: 241 EAGQATLKFHELDAVRWHVRELSIEGLADEAQWAGAVEQAVEEVRDAFPELMSVVRFRLT 300 + L FHELD++RW+V E I+ D +W ++E + + A + +S++R R+T Sbjct: 239 GSFNTKLSFHELDSMRWNVVEAPIDACRDVDEWRRSLESILTDTARANADRLSMIRVRVT 298 Query: 301 GRGAVHKALAEKGAAEDLLAELQRREAMRAERKAYAGLVWTEGFALETGLAVDRGRLVQE 360 GR VH+ L ++LL +L+RREA + + Y G VW E F+L++G A+D RL+ E Sbjct: 299 GRSPVHRQLEGGYVLDELLTDLRRREAAKGKTCGYRGCVWIESFSLQSGDAIDTERLLAE 358 Query: 361 DSFLGEMLRLAGRSEQSSAGLDELMDSALKPLMESRELRRMLASVTPEEKLGWLRNAAEL 420 DSF G++LRL + ++ D ++ L+PLME E+R +L +PEE L W+R A EL Sbjct: 359 DSFTGDLLRLVRKEQERIHDPDGVVAKGLEPLMEHAEIRSLLDETSPEEMLDWVRRAEEL 418 Query: 421 GISLL 425 + LL Sbjct: 419 TLGLL 423 >B1DBL9.1 B1DBL9_9BACL Metallophosphoesterase Length = 422 Score = 321 bits (822), Expect = 1e-85, Method: Compositional matrix adjust. Identities = 185/428 (43%), Positives = 250/428 (58%), Gaps = 16/428 (3%) Query: 2 IPFRFLHAADLHLDSRFAGLAQLPQAIRSYLRESTFAALGRLVSVAVQEKTDFVVISGDV 61 +PFRF+HAADLHLDS F G++++P A++ L STF+AL RL A+QE+ DF+VISGD+ Sbjct: 3 VPFRFIHAADLHLDSPFRGMSKVPDALKEKLMASTFSALRRLSDTAIQEQVDFIVISGDL 62 Query: 62 YDISDASLQGQLRFQEALQELGKHGIAVFLIHGNHDPLDGPRLSTEPPGHVTVFG--GSE 119 YD +D SL+ Q + + L ++GI+VF+IHGNHDPL+G + + P +VT FG G E Sbjct: 63 YDEADRSLKAQFLLLKEWERLQRYGISVFVIHGNHDPLNGAKAELKLPSNVTQFGANGME 122 Query: 120 PGQAVARRRSDGREVAVVSGISYPTAKVTENTALQFKRRPGSDLYHIALLHGNVDGDPQH 179 A R +G A V G+SY VTEN A ++ G+ +HIALLHGNV+GDP H Sbjct: 123 YRPAYCR---NGELAAFVYGMSYGKRHVTENIAARYSPVEGAP-FHIALLHGNVNGDPSH 178 Query: 180 ETYSPCSRKDLIGRGYDYWALGHIHKRRVLHEQPPIVYPGNIQGRSVKETGPKGCYVVDV 239 + Y+PC ++L G+G+DYWALGHIH R+VLHE P +VY GNIQGR+ +ETGPKGCYV +V Sbjct: 179 DPYAPCLLEELTGKGFDYWALGHIHTRKVLHEYPHVVYSGNIQGRNPRETGPKGCYVAEV 238 Query: 240 NEAGQATLKFHELDAVRWHVRELSIEGLADEAQWAGAVEQAVEEVRDAFPELMSVVRFRL 299 + + L F LD VRW ELSI GL E + +E+ VE++ +VR L Sbjct: 239 SASRAVQLTFIPLDDVRWLDAELSINGLHTEQELLQQMEELVEQLAAEGDGRPVMVRLTL 298 Query: 300 TGRGAVHKALAEKGAAEDLLAELQRREAMRAERKAYAGLVWTEGFALETGLAVDRGRLVQ 359 G+G +H L E LL +LQ+ W T + L + Sbjct: 299 EGQGPLHHKLTEMITVRTLLDQLQQGSLH----------AWVYALEGNTRAELRLSELEE 348 Query: 360 EDSFLGEMLRLAGRSEQSSAGLDELMDSALKPLMESRELRRMLASVTPEEKLGWLRNAAE 419 EDSF GE+ RLA R E+ S + +A+ PL +L R+L E WL A E Sbjct: 349 EDSFAGELFRLAQRLERDSEAWRQEAAAAVAPLSSHAKLGRLLRGHWDELPEHWLEQARE 408 Query: 420 LGISLLAG 427 + LL Sbjct: 409 HVLGLLTA 416 >Q8TNC7.1 Q8TNC7_METAC Phosphoesterase Length = 443 Score = 287 bits (735), Expect = 2e-75, Method: Compositional matrix adjust. Identities = 155/421 (36%), Positives = 243/421 (57%), Gaps = 10/421 (2%) Query: 6 FLHAADLHLDSRFAGLAQLPQAIRSYLRESTFAALGRLVSVAVQEKTDFVVISGDVYDIS 65 F+HAADLHLDS F G++ + Q + L ++TF A ++ + ++E+ DF++I+GDVYD + Sbjct: 24 FVHAADLHLDSPFVGISGIDQELGERLAKATFQAYEAIIELCMEEEVDFLLIAGDVYDSA 83 Query: 66 DASLQGQLRFQEALQELGKHGIAVFLIHGNHDPLDGPRLSTEPPGHVTVFGGSEPGQAVA 125 D +L Q+RF E L++L GI +F+ HGNHDPLDG S + P +V G + V Sbjct: 84 DKNLYAQVRFIEGLRKLETAGIQIFICHGNHDPLDGWSASLKWPANVHTMRGDK--AEVV 141 Query: 126 RRRSDGREVAVVSGISYPTAKVTENTALQFKRRPGSDLYHIALLHGNVDGDPQHETYSPC 185 + +G A V G+SYPT + +N F ++ + I LLH +V P+H+ Y+PC Sbjct: 142 EFKKEGETAAFVVGMSYPTRHIMKNLVQDFPKKEDHWPFTIGLLHCSVGSYPEHDPYAPC 201 Query: 186 SRKDLIGRGYDYWALGHIHKRRVLHEQPPIV-YPGNIQGRSVKETGPKGCYVVDVNEAGQ 244 + +DL GYDYWALGHIH V+ ++ P+V YPGN QGR ETG +GC +V+V+ G Sbjct: 202 TLQDLREPGYDYWALGHIHTPSVVCKEAPVVIYPGNPQGRHPGETGARGCCLVNVSSGGA 261 Query: 245 ATLKFHELDAVRWHVRELSIEGLADEAQWAGAVEQAVEEVRDAFPELMSVVRFRLTGRGA 304 + KF E D+VRWH+RE+SIEGL E + ++ ++E+R+ ++ R L+GRG Sbjct: 262 ISTKFVETDSVRWHIREISIEGLEKEGELVENLQSQLDEIRENSGGRSAICRLVLSGRGP 321 Query: 305 VHKALAEKGAAEDLLAELQRREAMRAERKAYAGLVWTEGFALETGLAVDRGRLVQEDSFL 364 +H L E+G EDLL L R + +R+ + W E ET ++R L++ + F+ Sbjct: 322 LHLTLREEGFLEDLLHTL-REDEIRSRQ-----FTWVERIENETLFPIERELLLKREDFV 375 Query: 365 GEMLRLAGRSEQSSAGLDELMDSALKPLMESRELRRMLASVTPEEKLGWLRNAAELGISL 424 G+++++ + LDE + L PL + + + + EE L+ A + + Sbjct: 376 GDLVKIVEGLKTDEEALDEF-HAVLAPLFKPGSGGKQICKIDDEEMKSLLQCAENILLDA 434 Query: 425 L 425 L Sbjct: 435 L 435 >Q46GD4.1 Q46GD4_METBF Phosphoesterase Length = 422 Score = 285 bits (729), Expect = 9e-75, Method: Compositional matrix adjust. Identities = 157/421 (37%), Positives = 243/421 (57%), Gaps = 10/421 (2%) Query: 6 FLHAADLHLDSRFAGLAQLPQAIRSYLRESTFAALGRLVSVAVQEKTDFVVISGDVYDIS 65 F+HAADLHLDS F G++++ + L ++TF A ++ + ++E+ DF++I+GDVYD + Sbjct: 3 FVHAADLHLDSPFTGISEIDPELGERLAKATFQAYEAIIKLCMEEEVDFLLIAGDVYDSA 62 Query: 66 DASLQGQLRFQEALQELGKHGIAVFLIHGNHDPLDGPRLSTEPPGHVTVFGGSEPGQAVA 125 D SL Q+RF E L++L GI VF+ HGNHDPLDG S P +V + G V Sbjct: 63 DKSLYAQVRFIEGLRKLETAGIQVFICHGNHDPLDGWSASLTWPENVHIMSGDR--AEVV 120 Query: 126 RRRSDGREVAVVSGISYPTAKVTENTALQFKRRPGSDLYHIALLHGNVDGDPQHETYSPC 185 + +G AV+ G+SYPT + +N F R+ + + I LLH +V +P+H+ Y+PC Sbjct: 121 EFKKEGETAAVIVGMSYPTRHIMKNLVKNFPRKEDNWPFTIGLLHCSVGSNPEHDPYAPC 180 Query: 186 SRKDLIGRGYDYWALGHIHKRR-VLHEQPPIVYPGNIQGRSVKETGPKGCYVVDVNEAGQ 244 + +DL YDYWALGHIH V E P ++YPGN QGR + E G +GC+VVDV+ G Sbjct: 181 TLQDLRKINYDYWALGHIHTPSIVCKEAPVVIYPGNPQGRHLGENGARGCFVVDVSSGGD 240 Query: 245 ATLKFHELDAVRWHVRELSIEGLADEAQWAGAVEQAVEEVRDAFPELMSVVRFRLTGRGA 304 + +F E D+VRW++RE+SIEGL E + +++ ++E+R+ + F L GRG Sbjct: 241 ISTRFMETDSVRWYIREISIEGLEKEGELIESLQSQLDEMRENSGGRSVICSFVLKGRGP 300 Query: 305 VHKALAEKGAAEDLLAELQRREAMRAERKAYAGLVWTEGFALETGLAVDRGRLVQEDSFL 364 +H + G EDLL L+ E + +W E +T V+R L++ + F+ Sbjct: 301 LHHIFRQDGFLEDLLRLLREGETWGRQ------FLWVERIEDDTLFPVERELLLKREDFV 354 Query: 365 GEMLRLAGRSEQSSAGLDELMDSALKPLMESRELRRMLASVTPEEKLGWLRNAAELGISL 424 G+++++ S+ LDE + AL PL SR R+ ++ + EE L+ A + + Sbjct: 355 GDLVKIVEGSKTDEKALDEFRE-ALSPLFGSRNGRKHISIIDDEEMNSLLQRAENILLDA 413 Query: 425 L 425 L Sbjct: 414 L 414 >Q12WJ5.1 Q12WJ5_METBU Metallophosphoesterase Length = 436 Score = 279 bits (713), Expect = 5e-73, Method: Compositional matrix adjust. Identities = 153/426 (35%), Positives = 239/426 (56%), Gaps = 9/426 (2%) Query: 6 FLHAADLHLDSRFAGLAQLPQAIRSYLRESTFAALGRLVSVAVQEKTDFVVISGDVYDIS 65 F+HAADLHLDS F GL+++ + + ++TF A ++ + +++ DF++ISGD+YD + Sbjct: 17 FVHAADLHLDSPFLGLSEINTKLSQVMAKATFDAYNNIIDLCIEKNADFLLISGDIYDSA 76 Query: 66 DASLQGQLRFQEALQELGKHGIAVFLIHGNHDPLDGPRLSTEPPGHVTVFGGSEPGQAVA 125 D SL QL+F E L+ L GIA ++ HGNHDPL+G S E P +V +F G + Sbjct: 77 DKSLYAQLKFLEGLKRLSSAGIAAYITHGNHDPLNGWSASLEWPANVKIFSGDDVETVFV 136 Query: 126 RRRSDGREVAVVSGISYPTAKVTENTALQFKRRPGSDLYHIALLHGNVDGDPQHETYSPC 185 + DG +A ++GISY T + EN A +F+++ + I +LH VD H Y+PC Sbjct: 137 EK--DGNVIASINGISYRTKHIQENLAKRFQKKDALSPFTIGMLHCTVDSGGGHYPYAPC 194 Query: 186 SRKDLIGRGYDYWALGHIHKRRVLHEQPPIVYPGNIQGRSVKETGPKGCYVVDVNEAGQA 245 S +DL YDYWALGHIH +VL P IVY GN QGR+ E G +GC +V+V++ G Sbjct: 195 SIQDLKETNYDYWALGHIHYPQVLETDPYIVYSGNPQGRNPGELGERGCTLVEVDQKGNI 254 Query: 246 TLKFHELDAVRWHVRELSIEGLADEAQWAGAVEQAVEEVRDAFPELMSVVRFRLTGRGAV 305 L F +D+VRW+V EL ++GL EA+ +E+ ++++ + + RF L GR + Sbjct: 255 KLDFVPVDSVRWYVEELYVDGLETEAELIELLEETMDKLSEIAEGRYIISRFILRGRSPL 314 Query: 306 HKALAEKGAAEDLLAELQRREAMRAERKAYAGLVWTEGFALETGLAVDRGRLVQEDSFLG 365 + L + + DL+ L R + +G VW E +T ++R L+ F+ Sbjct: 315 KRFLMKDDSLNDLVQHL------RDDYDVGSGSVWVERLQDKTSFPIERESLLNRQDFIS 368 Query: 366 EMLRLAGRSEQSSAGLDELMDSALKPLMESRELRRMLASVTPEEKLGWLRNAAELGISLL 425 ++L + + GL +L D +L PL ++R +L + +E++ RNA EL + L Sbjct: 369 DLLSITDEICSDNEGLMKL-DDSLHPLFGKGKIRHILMPLDEDERIRIARNAEELLLEKL 427 Query: 426 AGMDEY 431 EY Sbjct: 428 ISEGEY 433 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.134 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,000,766,761 Number of extensions: 127245841 Number of successful extensions: 350890 Number of sequences better than 1.0e-05: 413 Number of HSP's gapped: 350449 Number of HSP's successfully gapped: 414 Length of query: 508 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 368 Effective length of database: 1,741,754,103 Effective search space: 640965509904 Effective search space used: 640965509904 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)