BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0344.0001.p (373 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C4G3Y6.1 C4G3Y6_ABIDE Putative uncharacterized protein 202 4e-50 B1DFV7.1 B1DFV7_9BACL Extracellular solute-binding protein family 1 147 1e-33 B1DFV8.1 B1DFV8_9BACL Extracellular solute-binding protein family 1 131 1e-28 B8I998.1 B8I998_CLOCE Extracellular solute-binding protein family 1 110 2e-22 B1DCZ4.1 B1DCZ4_9BACL Extracellular solute-binding protein family 1 101 1e-19 >C4G3Y6.1 C4G3Y6_ABIDE Putative uncharacterized protein Length = 452 Score = 202 bits (514), Expect = 4e-50, Method: Compositional matrix adjust. Identities = 124/373 (33%), Positives = 195/373 (52%), Gaps = 6/373 (1%) Query: 1 VEELKKEMPDVEIELDSAVQDGNQKLKTYAATGNMPDVYFTDWPTLQTFAKSKNVELLDD 60 +E++K+ MP+V +E+++A QD QKL TYAA+GN+PDV+ +D ++ F KS + LDD Sbjct: 83 LEKMKEIMPNVTVEIEAAPQDDGQKLMTYAASGNLPDVFKSDGVGIELFNKSGALLQLDD 142 Query: 61 YESTAQYKQNLNPGVESRLIAPDGHVYAYPDTGIEFQLIYYNKSIFDKVGIAVPIKTIDG 120 Y A + ++ + L PDGH+YA P Y NK +F G+ +P + D Sbjct: 143 YVKKAGIEDSMVDSYKYALYMPDGHIYAVPWQSPWINSFYANKEVFKNNGVKIP-ENYDE 201 Query: 121 XXXXXXXXXXXGYIPMSIFGKEKWISAAFYNGLVTREQAAGFGALEAQGVTELPQAFVTA 180 +P ++FGKEK Y+ +VTRE+AAG AL+ + + +V A Sbjct: 202 FLAAVKAFRKKDILPYALFGKEKAWCLHLYDIMVTREKAAGIAALDRGEASATEEPYVKA 261 Query: 181 AEQMKQLQEAGLFDANATNTNYDQASSQFYQGKSAMFVNGQWEIYSSEEKLGDQVDWMYW 240 A ++ L + G+ + NT YDQA + F GK+AMF+ G W + EEK+GD++ +Y+ Sbjct: 262 ANKLLGLVKEGMLSQDVFNTTYDQAEAMFTSGKAAMFMTGTWALNGLEEKMGDKLALLYY 321 Query: 241 PAKDEATYEQSKYNIDGAGSPQGYAVSPGSKNKETAVKVAAFLAAKSAEYKYSKLGSPII 300 P D E ++N+ G S G++V SK+KE A + A L+ K E G I Sbjct: 322 PLADAGKEEDCRWNMSGGYSQGGFSVPSYSKHKEIAAEFAIRLSQKLNEAFVVLEGGASI 381 Query: 301 SPKTDKPIAADVPVMMQRIATELLPNAKEYAKL---LSNTQIQNAINDNTQNLLVDGFAT 357 A PVM Q +A N K + L+N +++ + D +Q LL + Sbjct: 382 YKSVPDTKAKLGPVMQQYVADN--KNFKSMSAFDWNLTNQELKTVMEDKSQELLTGSLSA 439 Query: 358 ENFITNLNRALGK 370 E+F+ +L+ A+ K Sbjct: 440 EDFVKDLDDAIKK 452 >B1DFV7.1 B1DFV7_9BACL Extracellular solute-binding protein family 1 Length = 436 Score = 147 bits (372), Expect = 1e-33, Method: Compositional matrix adjust. Identities = 86/283 (30%), Positives = 137/283 (48%), Gaps = 3/283 (1%) Query: 1 VEELKKEMPDVEIELDSAVQDGNQKLKTYAATGNMPDVYFTDWPTLQTFAKSKNV-ELLD 59 + E+ K+ P+V + + D ++ KT TG++P + + T++ S +V L Sbjct: 62 IAEVHKQFPNVTLIPEPLANDNGEQFKTRLVTGDLPAIMDINGQTIENVITSGSVIRLYS 121 Query: 60 DYESTAQYKQNLNPGVESRLIAPDGHVYAYPDTGIEFQLIYYNKSIFDKVGIAVPIKTID 119 + + Y L P V L DGH+YA+P G L+YYNK +F+ I VP Sbjct: 122 YFNAHPDYANQLIPSVADNLRYSDGHIYAFPRNGPAADLLYYNKRLFETYNIEVPTHYAQ 181 Query: 120 GXXXXXXXXXXXGYIPMSIFGKEKWISAAFYNGLVTREQAAGFGALEAQGVTELPQAFVT 179 IP+++F K+ W AF++ R G AL + Sbjct: 182 -LLTAVKVLRSHNIIPIAMFAKQAWPVGAFFDMFAMRANRDGMMALSQGTAKASDPGYKE 240 Query: 180 AAEQMKQLQEAGLFDANATNTNYDQASSQFYQGKSAMFVNGQWEIYSSEEKLGDQVDWM- 238 A +M +L + G+F NA T+Y A + FY GK+AMF++G+W+I +E LG VD+M Sbjct: 241 AIRKMGELIQNGVFQQNAIATDYVSAKALFYSGKAAMFISGEWDISDTEAHLGKDVDFMD 300 Query: 239 YWPAKDEATYEQSKYNIDGAGSPQGYAVSPGSKNKETAVKVAA 281 +P D+ + + + G G P G AV+ + N + AV+VAA Sbjct: 301 VFPTTDQGAVQANPLAMPGGGEPGGIAVAANAPNTDLAVQVAA 343 >B1DFV8.1 B1DFV8_9BACL Extracellular solute-binding protein family 1 Length = 464 Score = 131 bits (330), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 91/302 (30%), Positives = 148/302 (49%), Gaps = 10/302 (3%) Query: 2 EELKKEMPDVEIELDSAVQDGNQKLKTYAATGNMPDVYFTDWPTLQTFAKSKNVELLD-- 59 EELKK P+V I+ + + D LK ATG++PD +F P +Q A ++ +LD Sbjct: 85 EELKKIYPNVTIKYEPQMNDNGDSLKARIATGDLPD-FFNLMP-IQFDAAIQSGSILDLT 142 Query: 60 -DYESTAQYKQNLNPGVESRLIAPDGHVYAYPDTGIEFQLIYYNKSIFDKVGIAVPIKTI 118 ++++ + ++++L DG ++A P G +YYNK +F G+ VP Sbjct: 143 AAWDASTAKADMIQSSLDTQLKYTDGKIWAIPQNGPGIDPLYYNKKVFSDNGVKVPTNYP 202 Query: 119 DGXXXXXXXXXXXGYIPMSIFGKEKWISAAFYNGLVTREQAAGFGALEAQGVTELPQAFV 178 + G +P+ +F KE W AF++ R G AL + Sbjct: 203 E-LLEAVKAFHAAGIVPIPMFAKEAWPIGAFFDMFAMRVNPKGMMALNDGDIKASDPEMA 261 Query: 179 TAAEQMKQLQEAGLFDANATNTNYDQASSQFYQGKSAMFVNGQWEIYSSEEKLG---DQV 235 A +M +L +AG+F AT+ +YD A F+QGK+AM +NG+W+I + G + Sbjct: 262 DAIGKMAELIKAGIFQKGATSYDYDSARGLFHQGKAAMLINGEWDIGDMVKDFGADIGNI 321 Query: 236 DWM-YWPAKDEATYEQSKYNIDGAGSPQGYAVSPGSKNKETAVKVAAFLAAKSAEYKYSK 294 D++ +P D+ + +KY + GA G AVS K+ + A++VA LA A+ Y K Sbjct: 322 DFLDVYPTTDQGKEDANKYAMPGAAELGGLAVSSKVKDPDQAMQVAITLAKLIAQGGYVK 381 Query: 295 LG 296 G Sbjct: 382 EG 383 >B8I998.1 B8I998_CLOCE Extracellular solute-binding protein family 1 Length = 455 Score = 110 bits (275), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 89/297 (29%), Positives = 140/297 (47%), Gaps = 19/297 (6%) Query: 4 LKKEMPDVEIELD--SAVQDGNQKLKTYAATGNMPDVYFTDWPTLQTFAKSKNVELLDDY 61 L+KEMPD+E+E D QD KLK Y ++GNMPDV+F++ S N L Y Sbjct: 67 LEKEMPDLEVEFDLGGGGQDYANKLKVYNSSGNMPDVWFSEQNLSTVVIDSGNALDLAPY 126 Query: 62 ESTAQYKQNLNPGVESRLIAPD--GHVYAYPDTGIEFQL--IYYNKSIFDKVGIAVPIKT 117 ++ + E I PD G +Y+ F ++Y+K+IF + GI VP T Sbjct: 127 IYKWEFDKKF---TEKEFIMPDKNGRIYSVQSGNDMFTTPRLWYHKNIFKRYGIQVP-NT 182 Query: 118 IDGXXXXXXXXXXXGYIPMSIFGKEKW-ISAAFYNGLVTREQAAGFGALEA-QGVTELPQ 175 D G +P+SI G++ W ++ ++ E+ AL+ G T+ Sbjct: 183 YDELLKVCELLKVKGLVPISISGRDGWTLNLHLLQTMIMAEEPQ--VALDMLNGNTDFTN 240 Query: 176 AFV-TAAEQMKQLQEAGLFDANATNTNYDQASSQFYQGKSAMFVNGQWEIYSSEEKLGDQ 234 + A E++++L + G F N N +Y+ A + + G++AM WE+ EKL Sbjct: 241 PVIQRALERIRKLVQVGAFPYNTANLDYEPAINMYTSGRAAMLSAFSWEL-PRLEKLAPD 299 Query: 235 VDWMYWPAKDEATYEQSKYNIDGAGSP-QGYAVSPGSKNKETAVKVAAFLAAKSAEY 290 D+M WP+ T + + G+P GY V +KN E A + A + A + A Y Sbjct: 300 TDFMPWPSVRGGT--DNTKAVQYWGAPLSGYMVWAKTKNPEAAARFAMYCATQDALY 354 >B1DCZ4.1 B1DCZ4_9BACL Extracellular solute-binding protein family 1 Length = 457 Score = 101 bits (251), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 85/277 (30%), Positives = 127/277 (45%), Gaps = 22/277 (7%) Query: 1 VEELKKEMPDVEIELDSAVQDG-NQKLKTYAATGNMPDVYFTD-WPTLQTFAKSKNVELL 58 V++ + + P+V+IELDS D QKLKT AA+ +PD+ + +Q + +K L Sbjct: 83 VKQYETDHPNVKIELDSLNTDQQKQKLKTQAASHEVPDITIVNPAAQMQPYVDAKLFAPL 142 Query: 59 DDYESTAQYKQNLNPGVESRLIAPDGHVYAYPDTGIEFQLIYYNKSIFDKVGIAVPIKTI 118 +D K GV D ++YA PD G ++YYNK +F + GIA P T Sbjct: 143 NDMVEQNGLKDTFQEGVLD-FYTFDNNLYALPD-GNNIGVVYYNKDLFQQAGIANPPTTF 200 Query: 119 DGXXXXXXXXXXXGYIPMSIFGKEKWI-SAAFYNGLVTREQAAGF--GALEAQGVTELPQ 175 + G PM+I K+ W S F N L+ GF + + E P Sbjct: 201 EEMIADVKTLKAKGIQPMAIGEKDTWTGSFLFMNILLRTNGGPGFLSDVVAGKKTFEDP- 259 Query: 176 AFVTAAEQMKQLQEAGLFDANATNTNYDQASSQFYQGKSAMFVNGQWEIYSSE--EKLGD 233 AFV A + M+ L +AG F T+ +Y+ + F GK+AM+ G W E E L D Sbjct: 260 AFVEAVDAMQNLIQAGAFQEGVTSFDYNAGENIFKTGKAAMYYMGSWATGGIETSEALKD 319 Query: 234 QVDWMYWPAKDEATYEQSKYNIDGAGSPQGYAVSPGS 270 +V +P ++G G P + ++PGS Sbjct: 320 KVGVFKFPT------------VNGKGDPNQFMLAPGS 344 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.312 0.129 0.368 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,148,527,820 Number of extensions: 86432116 Number of successful extensions: 173173 Number of sequences better than 1.0e-05: 282 Number of HSP's gapped: 173244 Number of HSP's successfully gapped: 283 Length of query: 373 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 236 Effective length of database: 1,770,017,148 Effective search space: 417724046928 Effective search space used: 417724046928 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 132 (55.5 bits)