BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0342.0056.p (235 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q9RC26.1 Q9RC26_BACSY MrsG protein 69 3e-10 A7ZAH0.1 A7ZAH0_BACA2 MrsG 69 3e-10 C3AU28.1 C3AU28_BACMY Permease 62 4e-08 C3BJ19.1 C3BJ19_9BACI Permease 61 1e-07 C3B278.1 C3B278_BACMY Permease 61 1e-07 >Q9RC26.1 Q9RC26_BACSY MrsG protein Length = 247 Score = 68.9 bits (167), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 56/183 (30%), Positives = 87/183 (47%), Gaps = 16/183 (8%) Query: 63 LNTALLAGMIAGVEHESNSWKQLLTLPVSRAVFYWAKXXXXXXXXXXXXXXXXXXXXXXX 122 L L A +IA +EH++N WKQ L+ PVSR Y +K Sbjct: 68 LAATLSASIIANIEHQANGWKQTLSFPVSRMRIYVSKFICLVVSLFISSTIFLLGMVLLG 127 Query: 123 XXYTSE-PLPFQFLIRQIVYSFLASA-AVLGLQMWLSIQFSNQAIPMSIGLLGAVSGLFI 180 E +P+ L YS L + ++ Q+WLS+ FSNQA + +G + ++ GLF+ Sbjct: 128 KLVGFEGSVPWGLLFGD-SYSMLVTVLPIMAFQIWLSMVFSNQAFSILVGSVSSIMGLFL 186 Query: 181 ARDSSAALWTWVWPWAYPTLSSP-FMAHPG--------RWIGISAGLGVMLLAAGTLHFK 231 A AA T +P AYP+ SS + + G ++ IS LG+++L G++HF Sbjct: 187 A----AAQSTRWFPLAYPSQSSTVILQYEGIGYNPDLSSYLCISLFLGIIILFLGSIHFA 242 Query: 232 NKQ 234 + Sbjct: 243 KRD 245 >A7ZAH0.1 A7ZAH0_BACA2 MrsG Length = 252 Score = 68.9 bits (167), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 56/183 (30%), Positives = 87/183 (47%), Gaps = 16/183 (8%) Query: 63 LNTALLAGMIAGVEHESNSWKQLLTLPVSRAVFYWAKXXXXXXXXXXXXXXXXXXXXXXX 122 L L A +IA +EH++N WKQ L+ PVSR Y +K Sbjct: 73 LAATLSASIIANIEHQANGWKQTLSFPVSRMRIYVSKFICLVVSLFISSTIFLLGMVLLG 132 Query: 123 XXYTSE-PLPFQFLIRQIVYSFLASA-AVLGLQMWLSIQFSNQAIPMSIGLLGAVSGLFI 180 E +P+ L YS L + ++ Q+WLS+ FSNQA + +G + ++ GLF+ Sbjct: 133 KLVGFEGSVPWGLLFGD-SYSMLVTVLPIMAFQIWLSMVFSNQAFSILVGSISSIMGLFL 191 Query: 181 ARDSSAALWTWVWPWAYPTLSSP-FMAHPG--------RWIGISAGLGVMLLAAGTLHFK 231 A AA T +P AYP+ SS + + G ++ IS LG+++L G++HF Sbjct: 192 A----AAQSTRWFPLAYPSQSSTVILQYEGIGYNPDLSSYLCISLFLGIIILFLGSIHFA 247 Query: 232 NKQ 234 + Sbjct: 248 KRD 250 >C3AU28.1 C3AU28_BACMY Permease Length = 195 Score = 62.0 bits (149), Expect = 4e-08, Method: Compositional matrix adjust. Identities = 43/178 (24%), Positives = 79/178 (44%), Gaps = 14/178 (7%) Query: 61 MLLNTALLAGMIAGVEHESNSWKQLLTLPVSRAVFYWAKXXXXXXXXXXXXXXXXXXXXX 120 ++L ++ +IA +EH S+SWK LL+LP+++ + K Sbjct: 25 LILGITIVTSLIAHIEHHSSSWKHLLSLPINKRHIFLVKFILVFFLLAVSCSLLLIGIVG 84 Query: 121 XXXXYTSE-PLPFQFLIRQIVYSFLASAAVLGLQMWLSIQFSNQAIPMSIGLLGAVSGLF 179 + +P+ + + Y + ++ ++ LQ+W++I F NQ+I +IGLLG + +F Sbjct: 85 LGLALQFDASIPWLSIFKMSFYPYWSALPIVALQLWIAIIFQNQSIAFTIGLLGTIFTMF 144 Query: 180 IARDSSAAL---WTWVWPWAYPTLSSPFMAHPGRWIGISAGLGVMLLAAGTLHFKNKQ 234 S AL + W WP SP + + I +G+ +L T F + Sbjct: 145 -----SVALPNWFIWRWPSLRIDCGSPLLC-----VAIGLIVGICMLCIETADFTRRD 192 >C3BJ19.1 C3BJ19_9BACI Permease Length = 195 Score = 60.8 bits (146), Expect = 1e-07, Method: Compositional matrix adjust. Identities = 43/175 (24%), Positives = 81/175 (46%), Gaps = 8/175 (4%) Query: 61 MLLNTALLAGMIAGVEHESNSWKQLLTLPVSRAVFYWAKXXXXXXXXXXXXXXXXXXXXX 120 ++L ++ +IA +EH S+SWK LL+LP+++ + K Sbjct: 25 LILGITIVTSLIAHIEHHSSSWKHLLSLPINKRHIFLVKFILVFFLLAVSCSLLLIGIVG 84 Query: 121 XXXXYTSE-PLPFQFLIRQIVYSFLASAAVLGLQMWLSIQFSNQAIPMSIGLLGAVSGLF 179 + +P+ + + Y + ++ ++ LQ+W++I F NQ+I +IGLLG + +F Sbjct: 85 LGLALQFDASIPWLSIFKMSFYPYWSALPIVALQLWIAIIFQNQSIAFTIGLLGTIFTMF 144 Query: 180 IARDSSAALWTWVWPWAYPTLSSPFMAHPGRWIGISAGLGVMLLAAGTLHFKNKQ 234 S AL W + W +P+L + P + I +G+ +L T F + Sbjct: 145 -----SVALPNW-FIWRWPSLRIDW-GSPLLCVAIGLIVGICMLCIETADFTRRD 192 >C3B278.1 C3B278_BACMY Permease Length = 195 Score = 60.8 bits (146), Expect = 1e-07, Method: Compositional matrix adjust. Identities = 43/175 (24%), Positives = 81/175 (46%), Gaps = 8/175 (4%) Query: 61 MLLNTALLAGMIAGVEHESNSWKQLLTLPVSRAVFYWAKXXXXXXXXXXXXXXXXXXXXX 120 ++L ++ +IA +EH S+SWK LL+LP+++ + K Sbjct: 25 LILGITIVTSLIAHIEHHSSSWKHLLSLPINKRHIFLVKFILVFFLLAVSCSLLLIGIVG 84 Query: 121 XXXXYTSE-PLPFQFLIRQIVYSFLASAAVLGLQMWLSIQFSNQAIPMSIGLLGAVSGLF 179 + +P+ + + Y + ++ ++ LQ+W++I F NQ+I +IGLLG + +F Sbjct: 85 LGLALQFDASIPWLSIFKMSFYPYWSALPIVALQLWIAIIFQNQSIAFTIGLLGTIFTMF 144 Query: 180 IARDSSAALWTWVWPWAYPTLSSPFMAHPGRWIGISAGLGVMLLAAGTLHFKNKQ 234 S AL W + W +P+L + P + I +G+ +L T F + Sbjct: 145 -----SVALPNW-FIWRWPSLRIDW-GSPLLCVAIGLIVGICMLCIETADFTRRD 192 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.328 0.139 0.464 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,270,862,835 Number of extensions: 45597490 Number of successful extensions: 224251 Number of sequences better than 1.0e-05: 25 Number of HSP's gapped: 224237 Number of HSP's successfully gapped: 25 Length of query: 235 Length of database: 3,060,696,203 Length adjustment: 131 Effective length of query: 104 Effective length of database: 1,826,543,238 Effective search space: 189960496752 Effective search space used: 189960496752 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 129 (54.3 bits)