BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0342.0052.p (477 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q01XT5.1 Q01XT5_SOLUE Flotillin domain protein 335 5e-90 Q1IRU6.1 Q1IRU6_ACIBL Flotillin 320 2e-85 C4CNX9.1 C4CNX9_9CHLR Uncharacterized conserved protein 318 8e-85 Q67KD6.1 Q67KD6_SYMTH Flottilin 311 1e-82 C0V0Y6.1 C0V0Y6_9BACT Uncharacterized conserved protein 310 2e-82 >Q01XT5.1 Q01XT5_SOLUE Flotillin domain protein Length = 477 Score = 335 bits (859), Expect = 5e-90, Method: Compositional matrix adjust. Identities = 183/462 (39%), Positives = 287/462 (62%), Gaps = 13/462 (2%) Query: 9 YLISGIVVVILLALFSI-VNAYKKVPPNQAMIVYXXXXXXXXXXXXTFVIPGFQNNKTIS 67 ++I+G+++++L+ L ++ ++K P++A+IVY T + P +N + +S Sbjct: 6 FVIAGLMILVLMFLMAMFAKLFRKAGPHEAIIVYGFRGTRVVKGRGTVIFPMVENARGLS 65 Query: 68 MMLMSFDVIPAQAMFSQQGIKLNLEAVAQIKIKSDPTAILTASEQFIDRPEEDRETIILH 127 + LMSFDV P Q ++++QG+ + +EAVAQIK+KSDP +ILTA+EQF+ + E+RE +I Sbjct: 66 LELMSFDVAPKQDLYTRQGVAVTVEAVAQIKVKSDPESILTAAEQFLTKSPEEREGLIRL 125 Query: 128 SVEGHLRGLIGQLTVESILKTPDEINSKMRETCSEDLDKMGLEVVSFTIKKITDDKGYID 187 +EGHLRG+IGQLTVE I+K P+ + +MR TC++D+ KMGLEV+SFTIK++ D YI Sbjct: 126 VMEGHLRGIIGQLTVEEIVKQPEMVGDRMRSTCADDMTKMGLEVISFTIKEVRDKNEYIT 185 Query: 188 NMGVPEIERIRRDASIAKAEAERDIQIXXXXXXXXXXXXXXXXHQATIEAGTAARAKESQ 247 NMG P+I RI+RDA +A AEAERD I Q + A T ++AK+++ Sbjct: 186 NMGRPDIARIKRDADVATAEAERDTAIKRAVASRESAVAKAQADQERVLAETLSQAKQAE 245 Query: 248 FEKELNIKQADFKLETEVKKAQADLAYELQQNKIKQSLVTEQVKITQMEAEANRTVREIE 307 +++L +K+A++ + ++AQAD AYE+Q N ++Q + E+VKI Q+E E V++ E Sbjct: 246 SQRDLEVKKAEYLELVKKQQAQADKAYEIQGNIMQQQVRAEEVKIHQVEKEHEVEVQKAE 305 Query: 308 VELRQKELEATVIKPAQAENQATIMRAEAAKQRQILXXXXXXXXXXKRGLATAEAELAKG 367 + R+ EL ATV+K A+ E + A A K R I+ +G A AE KG Sbjct: 306 IARRENELIATVLKAAEYEKRRIETLAGAEKARLIMQAEGQASAIRAQGEAEAEIIFKKG 365 Query: 368 RANAEIVQXXXXXXXXXXXXXXXXYKQFTQAALTVEFLKVLPELAEKIASPLAKVDKITV 427 A A+ + Y++F QAA+ + + +PE+ +A+PLA VDKIT+ Sbjct: 366 EAEAKAMN-----------VKAEAYQEFNQAAIVDKLITNMPEVVRALAAPLANVDKITI 414 Query: 428 ISQ-DGASSGVNKITSDIAKIMTQVPELTQTLTGMNVTEALS 468 +S +GA+SG++KIT DI ++ Q+P L +TL+GM + E S Sbjct: 415 VSTGNGATSGMHKITGDITEMAAQIPALFETLSGMKMGELFS 456 >Q1IRU6.1 Q1IRU6_ACIBL Flotillin Length = 489 Score = 320 bits (821), Expect = 2e-85, Method: Compositional matrix adjust. Identities = 183/473 (38%), Positives = 283/473 (59%), Gaps = 20/473 (4%) Query: 5 MVMLYLISGIVVVI--------LLALFSIVNAYKKVPPNQAMIVYXXXXXXXXXXXXTFV 56 M +L I G VVVI + + + Y+K P++A++VY T + Sbjct: 1 MNLLSKIPGDVVVITGLIVVVVMFLMMMMARLYRKAGPHEALVVYGFRGTRIIKGKGTVI 60 Query: 57 IPGFQNNKTISMMLMSFDVIPAQAMFSQQGIKLNLEAVAQIKIKSDPTAILTASEQFIDR 116 P +N +S+ LMSFDV P Q ++++QG+ + +EAVAQIK+KSDP +I TASEQF+ + Sbjct: 61 FPMVENCLQLSLELMSFDVAPQQDLYTKQGVAVTVEAVAQIKVKSDPISIQTASEQFLTK 120 Query: 117 PEEDRETIILHSVEGHLRGLIGQLTVESILKTPDEINSKMRETCSEDLDKMGLEVVSFTI 176 + RE +I +EGHLRG+IGQLTVE I+K P+ + +MR TC++D+ KMGLEV+SFTI Sbjct: 121 TPQQREGLIRLVMEGHLRGIIGQLTVEEIVKQPEMVGDRMRATCADDMSKMGLEVISFTI 180 Query: 177 KKITDDKGYIDNMGVPEIERIRRDASIAKAEAERDIQIXXXXXXXXXXXXXXXXHQATIE 236 K++ D YI NMG P++ RI+RDA IA AEAERD I Q + Sbjct: 181 KEVRDKNQYITNMGRPDVARIKRDADIATAEAERDTAIKQAAAQREAAVARAQADQERVA 240 Query: 237 AGTAARAKESQFEKELNIKQADFKLETEVKKAQADLAYELQQNKIKQSLVTEQVKITQME 296 A TA++AK+++ +++L +K+A ++ + ++AQAD AYE+Q N ++Q ++ E VK+ Q+E Sbjct: 241 AETASQAKQAEAQRDLEVKRAAYQEMVKKQQAQADKAYEIQTNVMQQQVIAESVKVQQIE 300 Query: 297 AEANRTVREIEVELRQKELEATVIKPAQAENQATIMRAEAAKQRQILXXXXXXXXXXKRG 356 + V+E E+ +KEL ATV+K A+ E A A +QR ++ +G Sbjct: 301 KQEQVKVQEAEILRHEKELIATVLKGAEIEKARIETLASAERQRLMMEAEGRSSSIRAQG 360 Query: 357 LATAEAELAKGRANAEIVQXXXXXXXXXXXXXXXXYKQFTQAALTVEFLKVLPELAEKIA 416 A AE KG A A+ + ++++ QAA+ + L +PE+ +A Sbjct: 361 EAEAEIIFKKGEAEAKAMN-----------VKAEAFQEYNQAAVIDKLLSNMPEIVRALA 409 Query: 417 SPLAKVDKITVISQ-DGASSGVNKITSDIAKIMTQVPELTQTLTGMNVTEALS 468 +PL++VDKIT++S +G+S+G +KIT DIA++ QVP L + L+GM + + LS Sbjct: 410 TPLSQVDKITIVSTGNGSSAGAHKITGDIAEMAAQVPALFEALSGMKMADLLS 462 >C4CNX9.1 C4CNX9_9CHLR Uncharacterized conserved protein Length = 495 Score = 318 bits (815), Expect = 8e-85, Method: Compositional matrix adjust. Identities = 181/456 (39%), Positives = 284/456 (62%), Gaps = 11/456 (2%) Query: 15 VVVILLALFSIVNAYKKVPPNQAMIVYXXXXXXXXXXXXTFVIPGFQNNKTISMMLMSFD 74 V+ +LL + I Y++V PN+A+IVY V P FQ+ + +S+ LMSFD Sbjct: 13 VITVLLIMVVIGTMYRRVSPNRALIVYGAGGTRIVTGGGKLVWPLFQSYQELSLELMSFD 72 Query: 75 VIPAQAMFSQQGIKLNLEAVAQIKIKSDPTAILTASEQFIDRPEEDRETIILHSVEGHLR 134 V P+Q +++ QG+ +N+EAVAQIK+KSDP +I TA+EQF+ + +++RE +I +EGHLR Sbjct: 73 VAPSQDLYTSQGVAVNVEAVAQIKVKSDPESIRTAAEQFLTKTQQEREALIRLVMEGHLR 132 Query: 135 GLIGQLTVESILKTPDEINSKMRETCSEDLDKMGLEVVSFTIKKITDDKGYIDNMGVPEI 194 G++G LTVE I+K P+ + ++R+T ++DL KMGLEVVSFTIKK+ DD+ YI NMG P++ Sbjct: 133 GIVGLLTVEQIVKEPEMVAGRVRQTVADDLSKMGLEVVSFTIKKVMDDQDYIANMGRPDV 192 Query: 195 ERIRRDASIAKAEAERDIQIXXXXXXXXXXXXXXXXHQATIEAGTAARAKESQFEKELNI 254 RI+R+A IA+AEAERD I Q + A TA+ A++++ +++L I Sbjct: 193 ARIKREADIAQAEAERDTAIKRAMAMREAAIAQAQADQERVVAQTASEARQAEAQRDLEI 252 Query: 255 KQADFKLETEVKKAQADLAYELQQNKIKQSLVTEQVKITQMEAEANRTVREIEVELRQKE 314 K+A+++ + ++A A+ AY++ N+ +Q +V EQV+I Q++ + V+E+EV+ R++E Sbjct: 253 KRAEYEADVRRQRALAEKAYDIAANQAQQQVVAEQVRIEQVQRQEMIKVQELEVQRRERE 312 Query: 315 LEATVIKPAQAENQATIMRAEAAKQRQILXXXXXXXXXXKRGLATAEAELAKGRANAEIV 374 LEAT+IK A AE ++R L + AEA +G A AEI+ Sbjct: 313 LEATLIKQADAE-----------RRRIELLAEAERERRIREATGAAEAIRLEGLAEAEII 361 Query: 375 QXXXXXXXXXXXXXXXXYKQFTQAALTVEFLKVLPELAEKIASPLAKVDKITVISQDGAS 434 + ++++ QAA+ + L +PELA+ A LA VDK+T++S Sbjct: 362 RAKGQAEADAMHLRAAAFQEYNQAAVLDKLLTSMPELAQAFAQSLAGVDKVTIVSTGDGH 421 Query: 435 SGVNKITSDIAKIMTQVPELTQTLTGMNVTEALSGL 470 +G++ IT ++AK++ QVPEL +TLTG +V E LS L Sbjct: 422 NGISSITGELAKMIAQVPELFETLTGQSVVELLSRL 457 >Q67KD6.1 Q67KD6_SYMTH Flottilin Length = 515 Score = 311 bits (796), Expect = 1e-82, Method: Compositional matrix adjust. Identities = 185/457 (40%), Positives = 278/457 (60%), Gaps = 25/457 (5%) Query: 29 YKKVPPNQAMIVYXXXXXXXXXXXXTFVIPGFQNNKTISMMLMSFDVIPAQAMFSQQGIK 88 Y+KVPPN+A+IVY V P Q+ + +S+ LMSFDV+P Q ++ QG+ Sbjct: 25 YRKVPPNRALIVYGFGGPRVTKGGGLVVWPLIQSAQELSLELMSFDVVPQQDFYTVQGVA 84 Query: 89 LNLEAVAQIKIKSDPTAILTASEQFIDRPEEDRETIILHSVEGHLRGLIGQLTVESILKT 148 + +EAVAQIK+KSD +ILTA+EQF+ + +++ I+ +EGHLRG+IGQLTVE I+K Sbjct: 85 VTVEAVAQIKVKSDTESILTAAEQFLSKTTKEQNEILKLVMEGHLRGIIGQLTVEQIVKE 144 Query: 149 PDEINSKMRETCSEDLDKMGLEVVSFTIKKITDDKGYIDNMGVPEIERIRRDASIAKAEA 208 P+ + +MR ++D+ KMGLEV+SFTIK+I D YI+NMG P+ ERI+R A IA AEA Sbjct: 145 PEMVADRMRANVADDMSKMGLEVISFTIKEIKDKNDYINNMGRPDTERIKRAAEIAAAEA 204 Query: 209 ERDIQIXXXXXXXXXXXXXXXXHQATIEAGTAARAKESQFEKELNIKQADFKLETEVKKA 268 RD +I Q T+ A + + AK+++ +++LN+K+A F+ E + +A Sbjct: 205 LRDTEIKRAEAMREAAIAKARAEQETVLAQSESLAKQAEAQRDLNLKKAAFEAEVKRAQA 264 Query: 269 QADLAYELQQNKIKQSLVTEQVKITQMEAEANRTVREIEVELRQKELEATVIKPAQAENQ 328 QAD AY+++ N I+Q +V EQV++ Q+E + V+E E+ R+KEL ATV+K A+ E Q Sbjct: 265 QADKAYDIEANIIQQKVVAEQVRVQQVERQEQVKVQEAEILRREKELIATVLKAAEIERQ 324 Query: 329 ATIMRAEAAKQRQI------------LXXXXXXXXXXKRGLATAEAELAKGRANAEIVQX 376 A AA+ R++ L ++GLA AE LAKGRA AE ++ Sbjct: 325 KQEALA-AAQARKLEIEAEGQARAIRLSGEAEADVVRQKGLAEAEVILAKGRAEAEAMR- 382 Query: 377 XXXXXXXXXXXXXXXYKQFTQAALTVEFLKVLPELAEKIASPLAKVDKITVISQ-DGASS 435 YK++ QAA+ + + VLPEL A PL+++DK+T++SQ GA Sbjct: 383 ----------IKAEAYKEYGQAAILDKLMPVLPELMRAAAEPLSRIDKVTILSQGQGAEV 432 Query: 436 GVNKITSDIAKIMTQVPELTQTLTGMNVTEALSGLLG 472 GVNK +D+AK++ P + + LTG+++ E + L G Sbjct: 433 GVNKFMADVAKLVATAPTVLEGLTGISIQEMMKQLPG 469 >C0V0Y6.1 C0V0Y6_9BACT Uncharacterized conserved protein Length = 509 Score = 310 bits (795), Expect = 2e-82, Method: Compositional matrix adjust. Identities = 182/457 (39%), Positives = 286/457 (62%), Gaps = 13/457 (2%) Query: 29 YKKVPPNQAMIVYXXXXXXXXXXXXTFVIPGFQNNKTISMMLMSFDVIPAQAMFSQQGIK 88 ++KV PNQA+I+Y VIP Q+ + +S+ LMSFDV P + +++ QG+ Sbjct: 27 FRKVGPNQALIIYGLGGTHIVTGGGRLVIPMLQSARELSLELMSFDVSPERDLYTTQGVA 86 Query: 89 LNLEAVAQIKIKSDPTAILTASEQFIDRPEEDRETIILHSVEGHLRGLIGQLTVESILKT 148 +N+EAVAQIK+K+DPT+I TA+EQF+ + ++RE++I +EGHLRG+IGQLTVE I+K Sbjct: 87 VNVEAVAQIKVKNDPTSIKTAAEQFLTKSPQERESLIRLVMEGHLRGIIGQLTVEQIVKE 146 Query: 149 PDEINSKMRETCSEDLDKMGLEVVSFTIKKITDDKGYIDNMGVPEIERIRRDASIAKAEA 208 P+ ++ ++R +EDL KMGLE+VSFTIK++ D+ YI NMG P+I RI+++A+IA AEA Sbjct: 147 PEMVSDRVRANVAEDLSKMGLEIVSFTIKEVRDENEYIANMGKPDIARIQKEANIAAAEA 206 Query: 209 ERDIQIXXXXXXXXXXXXXXXXHQATIEAGTAARAKESQFEKELNIKQADFKLETEVKKA 268 RD I Q T+ A TA+ A++++ ++L +K+A++ + ++A Sbjct: 207 ARDTAIRQAETAREAAVAQALAQQETVIAQTASEARQAEARRDLELKKAEYLAAVKKQQA 266 Query: 269 QADLAYELQQNKIKQSLVTEQVKITQMEAEANRTVREIEVELRQKELEATVIKPAQAENQ 328 AD Y++Q N ++Q +V EQV++ ++E E V+E E+ R++EL+ATV+K A+AE Q Sbjct: 267 IADKTYDIQANVMQQQVVAEQVRVQRIEREEQIKVQEAEIARRERELQATVLKAAEAERQ 326 Query: 329 ATIMRAEAAKQRQILXXXXXXXXXXKRGLATAE---------AEL--AKGRANAEIVQXX 377 + AEA +QRQIL +G A AE AE+ A G A AEI++ Sbjct: 327 RIQLLAEAERQRQILEALGRAEAARTQGQAEAEVARVKGQAQAEVIRATGEAEAEIIKAK 386 Query: 378 XXXXXXXXXXXXXXYKQFTQAALTVEFLKVLPELAEKIASPLAKVDKITVISQ-DGAS-S 435 ++ + +AA+ + + LPE+ K++ PL++VD+ITVIS DGA+ S Sbjct: 387 GTSEAEAMRLKAEAFQGYNEAAILDKIITNLPEMLAKMSQPLSQVDRITVISTGDGANGS 446 Query: 436 GVNKITSDIAKIMTQVPELTQTLTGMNVTEALSGLLG 472 GVN++ D+A+++ P L ++LTGM + E L + G Sbjct: 447 GVNQVMQDLARMVAIAPTLVESLTGMKIAELLERVPG 483 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.314 0.129 0.333 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,938,398,347 Number of extensions: 62789919 Number of successful extensions: 213472 Number of sequences better than 1.0e-05: 568 Number of HSP's gapped: 212299 Number of HSP's successfully gapped: 569 Length of query: 477 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 337 Effective length of database: 1,741,754,103 Effective search space: 586971132711 Effective search space used: 586971132711 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 133 (55.8 bits)