BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0342.0040.p (561 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B8GE05.1 B8GE05_METPE Peptidase S41 227 2e-57 B7DCF6.1 B7DCF6_DESSA Peptidase S41 202 1e-49 A5UY65.1 A5UY65_ROSS1 Peptidase S41 162 7e-38 A7NN65.1 A7NN65_ROSCS Peptidase S41 156 5e-36 B3QVM0.1 B3QVM0_CHLT3 Carboxyl-terminal protease EC=3.4.21.102 73 7e-11 >B8GE05.1 B8GE05_METPE Peptidase S41 Length = 558 Score = 227 bits (579), Expect = 2e-57, Method: Compositional matrix adjust. Identities = 151/453 (33%), Positives = 232/453 (51%), Gaps = 36/453 (7%) Query: 126 SDLSHKSYTQAFIAMNERLSTEYPFGDWKKVDWAAKKNKYGPLFRQAEISQDPNLYYKTL 185 ++L++ S+++AF A N + Y F K VDW A Y P AE QD Y++ L Sbjct: 74 ANLTNMSWSEAFSAANSLMEERYAFSGLKAVDWDALYQTYAPAVAAAEKDQDKAAYFRAL 133 Query: 186 RDYLFSFRDGHIQIVNEHLYDGNSVFK-NEVGGGVGISTVQLDDGKVLVSLLIPGSPADQ 244 R Y+++ DGH+ ++ +G+ K ++GG G++ +LD G V+VS + S A+Q Sbjct: 134 RGYIYAIPDGHVSLLPA---EGDFGAKYADIGGDYGVAVTRLDSGTVIVSFVANRSQAEQ 190 Query: 245 YGIKLGAEIISWDGKDVKKALDETS--WSENPPAT-EGHRILNQGRFLARAPIGQNIQVK 301 GI+ G + +W+G+++ A++ TS W+ P+T EG R L+Q R L R PIG + Sbjct: 191 AGIRFGDVVTAWNGREILDAINTTSYIWAVKKPSTAEGIR-LHQQRLLTRGPIGSTATIT 249 Query: 302 FRNQDDPAIITAALTAYDDQYEXXXXXX-----------XXXXXED--PPVEGQ-----I 343 N T LTAYDD Y ED P + + Sbjct: 250 ISNSTASLPRTVTLTAYDDGYANLKQTSWFLGIPVNDYGVDRSWEDILPQISNETVTVRT 309 Query: 344 LKENYGYVKIRYFLPGATQSDPAKALEDTIKEFQEKQVKGIIIDLRDNPGGDDDLVAQMA 403 L NY Y+ + + D + + ++ GI+ DLR N GGDD L A A Sbjct: 310 LPGNYTYIAVY-----NEEYDVYQPFKAAMQNAIANNTPGIVFDLRFNSGGDDSLAACFA 364 Query: 404 GHFVNRKRIFEYVSYYNRNTGRFEINRAETRTVQPSRIYFPGKIAVLINHNTASSGEGLP 463 FV + +EY + Y+ GRF +E ++ P + G +AVL++ +T SSGEG+P Sbjct: 365 SWFVKKPAFYEYATKYDPGNGRF-TTLSEAWSI-PRADGYSGPVAVLVSPDTISSGEGVP 422 Query: 464 LILKGMPNVKIIGFTSTNGSFGIVSAPIEVKMPEGYVVQFPDGRSLNLDYKIQGDSDERG 523 +IL ++I F TNG+FG+ + ++ +P G + FPDG SL+L+ IQ DS+ Sbjct: 423 MILNRTGRGEVISFYGTNGAFGMNN--VQASLPLGLSLYFPDGASLDLNGTIQDDSNAGL 480 Query: 524 QGGVTPDITVPMNGQTFKMKFIEGQDVELDYAL 556 GGV+P I VP+N T + + G+DV+L YAL Sbjct: 481 TGGVSPGIRVPINEDTVA-RSMAGEDVQLTYAL 512 >B7DCF6.1 B7DCF6_DESSA Peptidase S41 Length = 497 Score = 202 bits (513), Expect = 1e-49, Method: Compositional matrix adjust. Identities = 144/459 (31%), Positives = 213/459 (46%), Gaps = 36/459 (7%) Query: 126 SDLSHKSYTQAFIAMNERLSTEYPFGDWKKVDWAAKKNKYGPLFRQAEISQDPNLYYKTL 185 SD S ++ A+ A++ + +Y FGDWK +DW A N P AE + +PN Y L Sbjct: 51 SDFSGMTWLAAYDALHALMQEQYAFGDWKSIDWNALNNTIRPKIILAENAANPNDYATAL 110 Query: 186 RDYLFSFRDGHIQIVNEHLYDGNSVFKNEVGGGVGISTVQLDDGKVLVSLLIPGSPADQY 245 +Y S DGH+ +E + + G +LD+GKV+ + + PA Sbjct: 111 LEYTRSIPDGHVMWGDEM----EKIIYPNIEESYGFGIAELDNGKVIATTVTALGPAASE 166 Query: 246 GIKLGAEIISWDGKDVKKALDETSWSENPP----ATEGHRILNQGRFLARAPIGQNIQVK 301 G+ G EI+ W+ + A ++S P AT + Q R L P +VK Sbjct: 167 GMLEGDEILEWNNVAITTAASQSSILWRPETASIATTELKRYEQFRALTLDPDANVSRVK 226 Query: 302 FRNQDDPAIITAALTAYDDQYEXXXXXXXXXXXEDP-PVEGQILKENYGYVKIRYFLPGA 360 F D + T LTA DD P++ IL YGY+ + G Sbjct: 227 FSRPDGSGVTTKDLTATDDGNTILQKTQFWNKINPADPIQHSILPSGYGYIVV-----GT 281 Query: 361 TQSDPAKALEDTIKEFQE-------KQVKGIIIDLRDNPGGDDDLVAQMAGHFVNRKRIF 413 +D + + +F+E V G+IIDLR N GG+D+L A+++G F + + Sbjct: 282 LDTD-NFSFDKLFNDFKEAMEFMATNNVPGLIIDLRGNGGGEDELAAKISGFFYAEETFY 340 Query: 414 EYVSYYNRNTGRFEI---NRAETR--------TVQPSRIYFPGKIAVLINHNTASSGEGL 462 EY + YN G EI NR T + P F G + L+N +T SS EG+ Sbjct: 341 EYQNQYNAYNGLMEIILPNRDGTAIIGWGIPLNIPPQTPLFTGPVVALVNPDTTSSAEGV 400 Query: 463 PLILKGMPNVKIIGFTSTNGSFGIVSAPIEVKMPEGYVVQFPDGRSLNLDYKIQGDSDER 522 + ++ + N ++G TNGSFG+ E KMP GY+V+FP+G+SLN D +IQ DS Sbjct: 401 AMTIQNLENGYVVGIFGTNGSFGMTGG--EAKMPMGYIVKFPNGQSLNQDREIQLDS-RN 457 Query: 523 GQGGVTPDITVPMNGQTFKMKFIEGQDVELDYALASFNS 561 G GG+TPDI V D+EL+ A++ S Sbjct: 458 GIGGITPDIKVSRTSANMINYVGRTADIELNRAVSFLQS 496 >A5UY65.1 A5UY65_ROSS1 Peptidase S41 Length = 692 Score = 162 bits (411), Expect = 7e-38, Method: Compositional matrix adjust. Identities = 134/440 (30%), Positives = 204/440 (46%), Gaps = 34/440 (7%) Query: 125 VSDLSHKSYTQAFIAMNERLSTEYPFGD--WKKVDWAAKKNKYGPLFRQAEISQDPNLYY 182 + D S SYT+AF AM R+ EY F K DW A + P +AE D ++ Sbjct: 271 IKDFSDLSYTKAFDAMFNRVRVEYAFNGIPGKAPDWDALYAELAPRVAEAERRADRRAFF 330 Query: 183 KTLRDYLFSFRDGHIQIVNEHLYDGNSVFKNEVGGGVGISTVQLDDGKVLVSLLIPGSPA 242 L D+ ++FRDGH+ + + ++F+ GG G + +LDDG+ LV + PA Sbjct: 331 DVLFDFAYAFRDGHVGVSSPL---SGALFRERASGGYGFAIRELDDGRALVMFVTRNGPA 387 Query: 243 DQYGIKLGAEIISWDGKDVKKALDETSWSENPPATEGHRILNQGRFLARAPIGQNIQVKF 302 D+ G+++GAE+++++G VK+A+ P +T+ Q R+L RAP+G + QV F Sbjct: 388 DRAGMQVGAELLAFNGTPVKEAIAAVEPLGGPFSTDFALRYQQARYLLRAPVGTSAQVTF 447 Query: 303 RN-QDDPAIITAALTAYDDQYEXXXXXXXXXXXEDP---PVEGQILKENYGYVKIRYFLP 358 N + P +T D + +P PVE + GY++I Sbjct: 448 ANPRSAPQTVTLRAVEERDSF----LATSIYEERNPAALPVEFEQRPSGVGYIRIN---- 499 Query: 359 GATQSD---PAKALEDTIKEFQEKQVKGIIIDLRDNPGGDDDLVAQMAGHFVNRKRIFEY 415 A D + E +K F + V GIIID+R N GG +AG +++ I Sbjct: 500 -ANYDDLNLLIRLFERALKTFDDLDVPGIIIDMRQNNGG---APLGLAGFLTDQEIIIGQ 555 Query: 416 VSYYNRNTGRFEINRAETRTVQPSRIYFPGKIAVLINHNTASSGEGLPLILKGMPNVKII 475 YY+ TGRFE + + Y KIA+L+ S+ E +P V ++ Sbjct: 556 DEYYSERTGRFEPEGPVDKILPNQNQYRFDKIALLVGQACFSACEYESYGFSRVPGVIVV 615 Query: 476 GFTSTNGSFGIVSAPIEVKMPEGYVVQFPDGRSLNLDYKIQGDSDERGQGGVTPDITVPM 535 G T T G + VS V +P+G +Q P GR+L D G G GV P I VP+ Sbjct: 616 GETPTAGVYAEVSRGQYV-LPDGIFLQVPTGRTLLPD----GTPLLEGV-GVVPTIRVPV 669 Query: 536 NGQTFKMKFIEGQDVELDYA 555 +T + +DV L+ A Sbjct: 670 TAET----VLSDRDVVLERA 685 >A7NN65.1 A7NN65_ROSCS Peptidase S41 Length = 691 Score = 156 bits (395), Expect = 5e-36, Method: Compositional matrix adjust. Identities = 131/427 (30%), Positives = 194/427 (45%), Gaps = 36/427 (8%) Query: 125 VSDLSHKSYTQAFIAMNERLSTEYPFG--DWKKVDWAAKKNKYGPLFRQAEISQDPNLYY 182 + D S SYT+AF M +R+ EY F K DW A P +AE QD ++ Sbjct: 270 IKDFSDLSYTRAFDEMFKRVRVEYAFNGIPGKAPDWDALYANLAPRVAEAERQQDRRAFF 329 Query: 183 KTLRDYLFSFRDGHIQIVNEHLYDGNSVFKNEVGGGVGISTVQLDDGKVLVSLLIPGSPA 242 + + D+ +FRDGH+ VN L ++F+ GG G + +LDDG+ LV + PA Sbjct: 330 EVMFDFANAFRDGHVG-VNSPL--SGALFRERAAGGYGFAIRELDDGRALVVFVTRNGPA 386 Query: 243 DQYGIKLGAEIISWDGKDVKKALDETSWSENPPATEGHRILNQGRFLARAPIGQNIQVKF 302 D+ G+++GAE+++++G VK A+ P +T+ Q R+L RAP+G QV F Sbjct: 387 DRAGVQVGAELLAFNGAPVKDAIAAVEPLGGPFSTDFALRYQQARYLLRAPVGTQAQVTF 446 Query: 303 RN-QDDPAIITAALTAYDDQYEXXXXXXXXXXXEDP---PVEGQILKENYGYVKIRYFLP 358 N + P +T D + +P PVE + GY++I Sbjct: 447 ANPRGAPQTVTLRAVEERDSF----FATSIFQESNPAALPVEFEQRASGVGYIRIN---- 498 Query: 359 GATQSDPAKAL----EDTIKEFQEKQVKGIIIDLRDNPGGDDDLVAQMAGHFVNRKRIFE 414 + D L E +K F + V GIIID+R N GG +AG +R+ I Sbjct: 499 --SNYDDLNLLIRLFERALKTFDDLDVPGIIIDMRQNSGG---APLGLAGFLSDREIIIG 553 Query: 415 YVSYYNRNTGRFEINRAETRTVQPSRIYFPGKIAVLINHNTASSGEGLPLILKGMPNVKI 474 YY+ TGRFE + Y KI +L+ S+ E +P V + Sbjct: 554 QDEYYSERTGRFEPEGPLDTILPHQNQYRFDKIVLLVGQACFSACEFESYGFSKVPGVIV 613 Query: 475 IGFTSTNGSFGIVSAPIEVKMPEGYVVQFPDGRSLNLDYK--IQGDSDERGQGGVTPDIT 532 IG T T G + VS V +P+ +Q P GR+L D ++G GV P I Sbjct: 614 IGETPTAGVYAEVSRGQYV-LPDDIFLQVPTGRTLLPDGAPLLEG-------VGVVPTIR 665 Query: 533 VPMNGQT 539 VP+ +T Sbjct: 666 VPVTAET 672 >B3QVM0.1 B3QVM0_CHLT3 Carboxyl-terminal protease EC=3.4.21.102 Length = 588 Score = 73.2 bits (178), Expect = 7e-11, Method: Compositional matrix adjust. Identities = 74/265 (27%), Positives = 123/265 (46%), Gaps = 41/265 (15%) Query: 217 GGVGISTVQLDDGKVLVSLLIPGSPADQYGIKLGAEIISWDGKDVKKALDETSWSENPPA 276 GVGIS + + D +V+V + G A++ G+++G IIS DG+DV+ Sbjct: 121 AGVGIS-INVKDNQVIVMSVAEGYSAEKAGVRIGDVIISIDGQDVR-------------- 165 Query: 277 TEGHRILNQGRFLARAPIGQNIQVKFRNQDDPAIITAALTAYDDQYEXXXXXXXXXXXED 336 G +L+ R L + I +Q+ + P I+ L +D + Sbjct: 166 --GRSVLDI-RNLIKGDINTEVQISVEREGLPKPISFQLVRHDVVLKNVTHV-------- 214 Query: 337 PPVEGQILKENYGYVKIRYFLPGATQS--DPAKALEDTIKEFQEKQVKGIIIDLRDNPGG 394 + K+ GYV I+ F A + D L+D+ K ++ Q+KG+I+DLRDNPGG Sbjct: 215 -----DLGKDGIGYVDIQRFSVKAAEELEDAIFMLQDSAKA-RKTQMKGLILDLRDNPGG 268 Query: 395 DDDLVAQMAGHFVNRKRIFEYVSYYNRNTGRFEINRAETRTVQPSRIYFPGKIAVLINHN 454 D+ +AG FV K+ V+ R++ + R+ T P P + VLIN + Sbjct: 269 LLDVAVSVAGKFV--KKGSTIVTTRGRDSVKV---RSYVSTTPPLLKDLP--VVVLINKS 321 Query: 455 TASSGEGLPLILKGMPNVKIIGFTS 479 +AS+ E + ++ + I+G S Sbjct: 322 SASASEIVAGAVQDLDRGVIVGTRS 346 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.140 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,499,345,239 Number of extensions: 154766270 Number of successful extensions: 361273 Number of sequences better than 1.0e-05: 242 Number of HSP's gapped: 361747 Number of HSP's successfully gapped: 243 Length of query: 561 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 420 Effective length of database: 1,732,333,088 Effective search space: 727579896960 Effective search space used: 727579896960 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)