BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0342.0010.p (160 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q2J8X0.1 Q2J8X0_FRASC 3-demethylubiquinone-9 3-methyltransferase 221 1e-56 Q0RN75.1 Q0RN75_FRAAA Putative uncharacterized protein 214 2e-54 B5GN48.1 B5GN48_STRCL 3-demethylubiquinone-9 3-methyltransferase 214 3e-54 A4FEG9.1 A4FEG9_SACEN 3-demethylubiquinone-9 3-methyltransferase 213 6e-54 Q21QZ7.1 Q21QZ7_RHOFD 3-demethylubiquinone-9 3-methyltransferase 213 6e-54 >Q2J8X0.1 Q2J8X0_FRASC 3-demethylubiquinone-9 3-methyltransferase Length = 157 Score = 221 bits (564), Expect = 1e-56, Method: Compositional matrix adjust. Identities = 102/155 (65%), Positives = 118/155 (76%) Query: 1 MPKIVTNLWFDTEAEEAAKYYTSIFKNSRITNTAYYGEGGRRPAGTVLTVTFELDGQVYT 60 MP I LWFDT+ EEAA++YTSIF NSRI + YG G RP GTV+TV FELDGQ Y Sbjct: 1 MPAITPCLWFDTQGEEAARFYTSIFPNSRIVDITRYGPAGPRPEGTVMTVRFELDGQEYV 60 Query: 61 ALNGGPEFKFTEAISLLVNCEGQEEVDELWEKLSAGGTEGPCGWLKDRYGLSWQIFPTVL 120 ALNGGPEF F+E ISL V C Q E+DE W++L+ GG GPCGWLKDRYGLSWQI PT L Sbjct: 61 ALNGGPEFTFSEGISLQVGCGSQAEIDEYWDRLTDGGEAGPCGWLKDRYGLSWQIVPTAL 120 Query: 121 GELLNDPDPVKAQRVMQAMLQMGKLDIAALKRAHE 155 ELL+DPDP ++QR MQAML+M KLDI L+RA + Sbjct: 121 SELLDDPDPGRSQRAMQAMLKMSKLDIEELRRAAD 155 >Q0RN75.1 Q0RN75_FRAAA Putative uncharacterized protein Length = 157 Score = 214 bits (545), Expect = 2e-54, Method: Compositional matrix adjust. Identities = 102/157 (64%), Positives = 118/157 (75%) Query: 1 MPKIVTNLWFDTEAEEAAKYYTSIFKNSRITNTAYYGEGGRRPAGTVLTVTFELDGQVYT 60 M I LWFD + E+AA++YTSIF NS+ITN A YG GG RP GT +TV FELDG+ Y Sbjct: 1 MSSITPCLWFDDQGEQAAEFYTSIFPNSKITNIARYGPGGMRPEGTAMTVQFELDGREYV 60 Query: 61 ALNGGPEFKFTEAISLLVNCEGQEEVDELWEKLSAGGTEGPCGWLKDRYGLSWQIFPTVL 120 ALNGGP F FTEAISL V C Q EVDE W +L+ GG E CGWLKDRYGLSWQI PT L Sbjct: 61 ALNGGPAFTFTEAISLQVFCGSQAEVDEYWARLTDGGEESQCGWLKDRYGLSWQIVPTAL 120 Query: 121 GELLNDPDPVKAQRVMQAMLQMGKLDIAALKRAHEGS 157 GELL DPDP +A+R +AML+M K+DIA L+RA EG+ Sbjct: 121 GELLGDPDPDRARRATEAMLRMKKIDIAELRRAAEGA 157 >B5GN48.1 B5GN48_STRCL 3-demethylubiquinone-9 3-methyltransferase Length = 159 Score = 214 bits (544), Expect = 3e-54, Method: Compositional matrix adjust. Identities = 97/158 (61%), Positives = 121/158 (76%), Gaps = 1/158 (0%) Query: 1 MPKIVTNLWFDTEAEEAAKYYTSIFKNSRITNTAYYGEGGRRPAGTVLTVTFELDGQVYT 60 MP+I+ NLWFDT+ +EAA++Y S+F NSRIT +YGE G PAGTV+TV FELDG Sbjct: 1 MPRIIPNLWFDTQGKEAAEFYCSVFPNSRITRVTHYGEAGPLPAGTVMTVDFELDGLPCV 60 Query: 61 ALNGGPEFKFTEAISLLVNCEGQEEVDELWEKLSA-GGTEGPCGWLKDRYGLSWQIFPTV 119 ALN GP F F EA+SLL+ C Q EVD WE L+A GG+EGPCGWLKDRYGLSWQ+ P + Sbjct: 61 ALNAGPAFTFNEAVSLLIECADQAEVDRYWELLTADGGSEGPCGWLKDRYGLSWQVVPKM 120 Query: 120 LGELLNDPDPVKAQRVMQAMLQMGKLDIAALKRAHEGS 157 + +LL+DPDP + QR M+AM+ M KLDIAAL+ A +G+ Sbjct: 121 MEDLLSDPDPARTQRAMRAMMTMRKLDIAALQAAADGT 158 >A4FEG9.1 A4FEG9_SACEN 3-demethylubiquinone-9 3-methyltransferase Length = 156 Score = 213 bits (541), Expect = 6e-54, Method: Compositional matrix adjust. Identities = 101/155 (65%), Positives = 119/155 (76%) Query: 1 MPKIVTNLWFDTEAEEAAKYYTSIFKNSRITNTAYYGEGGRRPAGTVLTVTFELDGQVYT 60 M KI T LWFD +AEEAA++YTSIF +SRI + YGE G P G+V+TV FEL GQ + Sbjct: 1 MQKITTFLWFDGQAEEAAEFYTSIFPDSRIAHVQRYGEAGPGPEGSVMTVNFELAGQEFI 60 Query: 61 ALNGGPEFKFTEAISLLVNCEGQEEVDELWEKLSAGGTEGPCGWLKDRYGLSWQIFPTVL 120 ALNGGP F+FTEAISL V+CE Q+EVDELW KL+ GG E CGWL DRYGLSWQI PTVL Sbjct: 61 ALNGGPRFRFTEAISLFVDCESQKEVDELWAKLTDGGEESRCGWLNDRYGLSWQIVPTVL 120 Query: 121 GELLNDPDPVKAQRVMQAMLQMGKLDIAALKRAHE 155 ++L DPDP ++ RVM+AML MGKLDI L A+E Sbjct: 121 MDMLKDPDPARSARVMKAMLGMGKLDIQRLVDAYE 155 >Q21QZ7.1 Q21QZ7_RHOFD 3-demethylubiquinone-9 3-methyltransferase Length = 160 Score = 213 bits (541), Expect = 6e-54, Method: Compositional matrix adjust. Identities = 97/153 (63%), Positives = 119/153 (77%) Query: 1 MPKIVTNLWFDTEAEEAAKYYTSIFKNSRITNTAYYGEGGRRPAGTVLTVTFELDGQVYT 60 M KI LWFD++AEEA +Y SIF+NS++ YGE G P GTV+T +F LDGQ + Sbjct: 1 MQKITPFLWFDSQAEEAMNFYVSIFRNSKVLTVNRYGEAGPGPRGTVMTASFLLDGQEFV 60 Query: 61 ALNGGPEFKFTEAISLLVNCEGQEEVDELWEKLSAGGTEGPCGWLKDRYGLSWQIFPTVL 120 ALNGGP++KFT AIS +VNCE Q EVD+LW+KLSAGG E C WL+D++G+SWQI P VL Sbjct: 61 ALNGGPQYKFTPAISFVVNCETQAEVDDLWDKLSAGGREDQCAWLQDKFGVSWQIVPRVL 120 Query: 121 GELLNDPDPVKAQRVMQAMLQMGKLDIAALKRA 153 ELLNDPDP KAQRVM AM++M K+DIAAL+RA Sbjct: 121 IELLNDPDPAKAQRVMAAMMKMKKIDIAALQRA 153 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.135 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,032,067,148 Number of extensions: 42900646 Number of successful extensions: 96028 Number of sequences better than 1.0e-05: 482 Number of HSP's gapped: 95161 Number of HSP's successfully gapped: 510 Length of query: 160 Length of database: 3,060,696,203 Length adjustment: 121 Effective length of query: 39 Effective length of database: 1,920,753,388 Effective search space: 74909382132 Effective search space used: 74909382132 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 125 (52.8 bits)